################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 828 GTATAC GT^[AC][GT]AC AcsI 232 GAATTT [GA]^AATT[CT] AflII 1288 CTTAAG C^TTAAG AflIII 867 ACATGT A^C[GA][CT]GT Alw44I 935 GTGCAC G^TGCAC AlwNI 694 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 935 GTGCAC G^TGCAC ApoI 232 GAATTT [GA]^AATT[CT] AspHI 935 GTGCAC G[AT]GC[AT]^C AvaII 12 GGTCC G^G[AT]CC BalI 143 TGGCCA TGG^CCA BamHI 814 GGATCC G^GATCC BfaI 200 CTAG C^TAG BfrI 1288 CTTAAG C^TTAAG BglII 287 AGATCT A^GATCT BsaAI 1159 TACGTG [CT]AC^GT[GA] BseRI 166 GAGGAG GAGGAG(10/8) BsgI 792 CTGCAC GTGCAG(16/14) BsiHKAI 935 GTGCAC G[AT]GC[AT]^C BsmAI 1010 GAGAC GTCTC(1/5) BsmFI 864 GGGAC GGGAC(10/14) BspLU11I 867 ACATGT A^CATGT BsrBI 416 GAGCGG CCGCTC(-3/-3) BsrDI 489 CATTGC GCAATG(2/0) BssSI 329 CACGAG CACGAG(-5/-1) Bst1107I 828 GTATAC GTA^TAC BstAPI 1024 GCATATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 909 CCAGGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 828 GTATAC GTA^TAC CfoI 156 GCGC GCG^C DrdI 576 GACTCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 507 CCATGG C^C[GA][CT]GG EaeI 143 TGGCCA [CT]^GGCC[GA] HhaI 156 GCGC GCG^C HinP1I 156 GCGC G^CGC HindIII 1087 AAGCTT A^AGCTT MaeI 200 CTAG C^TAG MaeIII 1135 GTCAC ^GT[ACGT]AC MluNI 143 TGGCCA TGG^CCA MscI 143 TGGCCA TGG^CCA MspA1I 676 CAGCTG C[AC]G^C[GT]G NcoI 507 CCATGG C^CATGG NdeI 1025 CATATG CA^TATG NspI 867 ACATGT [GA]CATG^[CT] PaeR7I 524 CTCGAG C^TCGAG PstI 700 CTGCAG CTGCA^G PvuII 676 CAGCTG CAG^CTG SfcI 700 CTGCAG C^T[GA][CT]AG TaqI 525 TCGA T^CGA Tsp45I 1135 GTCAC ^GT[GC]AC TthHB8I 525 TCGA T^CGA XbaI 199 TCTAGA T^CTAGA XhoI 524 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaI 524 CTCGAG C^[CT]CG[GA]G 752 CTCGGG BanI 97 GGTGCC G^G[CT][GA]CC 307 GGTGCC BanII 994 GGGCTC G[GA]GC[CT]^C 1036 GGGCTC BsoBI 524 CTCGAG C^[CT]CG[GA]G 752 CTCGGG Cfr13I 12 GGTCC G^G[ACGT]CC 1223 GGCCC DraI 213 TTTAAA TTT^AAA 445 TTTAAA EcoT14I 507 CCATGG C^C[AT][AT]GG 1184 CCAAGG HphI 1054 GGTGA GGTGA(8/7) 239 TCACC MwoI 484 GCTGACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1024 GCATATGGTGC Sau96I 12 GGTCC G^G[ACGT]CC 1223 GGCCC ScaI 644 AGTACT AGT^ACT 1064 AGTACT SfaNI 427 GATGC GCATC(5/9) 1180 GATGC StyI 507 CCATGG C^C[AT][AT]GG 1184 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SmlI TfiI Tru9I TseI Tsp509I TspRI XcmI XhoII