(Circular) (Six-base) MAPSORT of: pFN21AE4434.seq Check: 1979 from: 1 to: 6061 pFN21AE4434 6061 bp With 182 enzymes: SgfI * March 28, 2012 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2834 3404 1937 Size: 897 570 4594 Fragments arranged by size: 4594 897 570 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 4124 5508 5776 1632 Size: 974 1518 1384 268 1917 Fragments arranged by size: 1917 1518 1384 974 268 AflII C'TTAA_G Cuts at: 820 1017 3293 820 Size: 197 2276 3588 Fragments arranged by size: 3588 2276 197 AflIII A'CryG_T Cuts at: 1203 2872 4960 5937 1203 Size: 1669 2088 977 1327 Fragments arranged by size: 2088 1669 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2704 3804 5376 2704 Size: 1100 1572 3389 Fragments arranged by size: 3389 1572 1100 ApaLI G'TGCA_C Cuts at: 2940 4118 5274 2940 Size: 1178 1156 3727 Fragments arranged by size: 3727 1178 1156 ApoI r'AATT_y Cuts at: 1493 2237 3370 3596 3748 5857 1493 Size: 744 1133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2529 2757 3386 1870 Size: 73 586 228 629 4545 Fragments arranged by size: 4545 629 586 228 73 BamHI G'GATC_C Cuts at: 2819 3391 3758 2819 Size: 572 367 5122 Fragments arranged by size: 5122 572 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2312 3382 4837 618 Size: 325 217 627 315 210 1070 1455 1842 Fragments arranged by size: 1842 1455 1070 627 325 315 217 210 BanII G_rGCy'C Cuts at: 1391 3003 3045 3380 1391 Size: 1612 42 335 4072 Fragments arranged by size: 4072 1612 335 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2694 2782 3122 928 Size: 971 189 606 88 340 3867 Fragments arranged by size: 3867 971 606 340 189 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2292 6056 2292 Size: 3764 2297 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2361 2570 2880 2944 3346 4122 5278 1790 Size: 571 209 310 64 402 776 1156 2573 Fragments arranged by size: 2573 1156 776 571 402 310 209 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2899 3792 4750 Size: 416 53 83 399 24 1482 893 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1482 958 893 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3125 3131 3332 4721 1239 Size: 1886 6 201 1389 2579 Fragments arranged by size: 2579 1886 1389 201 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2317 4186 5051 5349 2317 Size: 1869 865 298 3029 Fragments arranged by size: 3029 1869 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3166 3852 493 Size: 711 1962 686 2702 Fragments arranged by size: 2702 1962 711 686 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 1186 Size: 1000 5061 BseYI C'CCAG_C Cuts at: 2679 3038 3285 5264 5760 2679 Size: 359 247 1979 496 2980 Fragments arranged by size: 2980 1979 496 359 247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2782 1163 Size: 573 1046 4442 Fragments arranged by size: 4442 1046 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2944 4122 4207 5278 2944 Size: 1178 85 1071 3727 Fragments arranged by size: 3727 1178 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2570 2880 2944 3003 3045 3346 Size: 399 571 209 310 64 59 42 301 Cuts at: 3346 3380 4122 4207 5278 1391 Size: 34 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 310 301 209 85 64 59 42 34 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2423 3948 1394 Size: 429 600 1525 3507 Fragments arranged by size: 3507 1525 600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 4556 4730 65 Size: 1183 1245 2063 174 1396 Fragments arranged by size: 2063 1396 1245 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 2334 4121 5133 2334 Size: 1787 1012 3262 Fragments arranged by size: 3262 1787 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3035 794 Size: 2241 3820 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2921 2921 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2292 2455 2819 3391 3758 4143 4160 5612 Size: 320 163 364 572 367 385 17 1452 Cuts at: 5612 5698 5710 6056 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 572 385 367 364 346 320 163 86 17 12 BstZ17I GTA'TAC Cuts at: 2835 2835 Size: 6061 BtgI C'CryG_G Cuts at: 513 1065 2512 513 Size: 552 1447 4062 Fragments arranged by size: 4062 1447 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2010 2794 3346 3577 4371 4391 2010 Size: 784 552 231 794 20 3680 Fragments arranged by size: 3680 794 784 552 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 2220 2452 3365 2220 Size: 232 913 4916 Fragments arranged by size: 4916 913 232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2587 5068 809 Size: 507 1271 2481 1802 Fragments arranged by size: 2481 1802 1271 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 3508 4397 Size: 54 1425 50 275 18 318 1360 889 Cuts at: 4397 6026 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1360 889 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3991 1353 Size: 2638 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2714 4648 5022 5168 387 Size: 1064 138 1125 1934 374 146 1280 Fragments arranged by size: 1934 1280 1125 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2606 3125 3131 3332 4124 4721 5508 Size: 393 974 519 6 201 792 597 787 Cuts at: 5508 5776 1239 Size: 268 1524 Fragments arranged by size: 1524 974 792 787 597 519 393 268 201 6 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373 Size: 32 135 32 3130 32 42 32 2626 Fragments arranged by size: 3130 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 3092 748 Size: 2344 3717 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2755 5175 5359 1305 Size: 1450 2420 184 2007 Fragments arranged by size: 2420 2007 1450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 10 Size: 54 1425 343 318 3921 Fragments arranged by size: 3921 1425 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 4019 4378 4537 518 Size: 648 2853 359 159 2042 Fragments arranged by size: 2853 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 3477 4154 5302 5547 1606 Size: 1077 794 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 794 677 245 NcoI C'CATG_G Cuts at: 513 1065 2512 513 Size: 552 1447 4062 Fragments arranged by size: 4062 1447 552 NdeI CA'TA_TG Cuts at: 387 3031 387 Size: 2644 3417 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2876 3419 4964 1132 Size: 97 1647 543 1545 2229 Fragments arranged by size: 2229 1647 1545 543 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 2872 4960 2872 Size: 2088 3973 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PfoI T'CCnGG_A Cuts at: 2019 2019 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspXI vC'TCGA_Gb Cuts at: 2529 2529 Size: 6061 PstI C_TGCA'G Cuts at: 830 2709 3413 830 Size: 1879 704 3478 Fragments arranged by size: 3478 1879 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2683 2683 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 2651 3071 4309 6021 2651 Size: 420 1238 1712 2691 Fragments arranged by size: 2691 1712 1238 420 SfcI C'TryA_G Cuts at: 826 1045 2705 3409 4544 5225 5416 826 Size: 219 1660 704 1135 681 191 1471 Fragments arranged by size: 1660 1471 1135 704 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2296 2529 3293 4165 5066 5328 Size: 197 926 353 233 764 872 901 262 Cuts at: 5328 820 Size: 1553 Fragments arranged by size: 1553 926 901 872 764 353 262 233 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2512 3189 3500 513 Size: 552 1447 677 311 3074 Fragments arranged by size: 3074 1447 677 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 2759 2927 3272 4088 4105 4258 Size: 1255 681 498 168 345 816 17 153 Cuts at: 4258 4443 4888 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 816 681 498 445 345 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 2649 2805 Size: 274 80 33 51 1443 642 30 156 Cuts at: 2805 3069 4307 6019 96 Size: 264 1238 1712 138 Fragments arranged by size: 1712 1443 1238 642 274 264 156 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 2204 3397 2204 Size: 1193 4868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2383 2530 2017 Size: 366 147 5548 Fragments arranged by size: 5548 366 147 XhoI C'TCGA_G Cuts at: 1943 2529 1943 Size: 586 5475 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI