Restriction map for
pFN21AE3984
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsoBI BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI HphI KasI KpnI
KspI MaeIII MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 183 GTCGAC GT^[AC][GT]AC
AciI 282 CCGC CCGC(-3/-1)
AlwI 295 GGATC GGATC(4/5)
AlwNI 122 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 571 ATTAAT AT^TAAT
AsnI 571 ATTAAT AT^TAAT
AspHI 56 GAGCAC G[AT]GC[AT]^C
AvaII 73 GGACC G^G[AT]CC
BalI 419 TGGCCA TGG^CCA
BbrPI 494 CACGTG CAC^GTG
BfaI 356 CTAG C^TAG
BmyI 56 GAGCAC G[AGT]GC[ACT]^C
BpmI 276 CTGGAG CTGGAG(16/14)
BsaAI 494 CACGTG [CT]AC^GT[GA]
BsaJI 291 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 56 GAGCAC G[AT]GC[AT]^C
BsmFI 72 GGGAC GGGAC(10/14)
BsmI 222 GAATGC GAATGC(1/-1)
Bsp1286I 56 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 32 TGTACA T^GTACA
BspMI 81 ACCTGC ACCTGC(4/8)
BsrGI 32 TGTACA T^GTACA
BssKI 102 CCTGG ^CC[ACGT]GG
BstNI 102 CCTGG CC^[AT]GG
BstYI 295 GGATCT [GA]^GATC[CT]
Cfr13I 73 GGACC G^G[ACGT]CC
EaeI 419 TGGCCA [CT]^GGCC[GA]
EcoRII 102 CCTGG ^CC[AT]GG
EcoT14I 291 CCAAGG C^C[AT][AT]GG
HincII 183 GTCGAC GT[CT]^[GA]AC
HindII 183 GTCGAC GT[CT]^[GA]AC
HinfI 510 GATTC G^A[ACGT]TC
MaeI 356 CTAG C^TAG
MflI 295 GGATCT [GA]^GATC[CT]
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
MslI 389 CACAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 565 CAGCTG C[AC]G^C[GT]G
MvaI 102 CCTGG CC^[AT]GG
MwoI 158 GCAGTGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI 494 CACGTG CAC^GTG
PmlI 494 CACGTG CAC^GTG
PshBI 571 ATTAAT AT^TAAT
PstI 156 CTGCAG CTGCA^G
PvuII 565 CAGCTG CAG^CTG
SalI 183 GTCGAC G^TCGAC
SapI 376 GCTCTTC GCTCTTC(1/4)
Sau96I 73 GGACC G^G[ACGT]CC
ScrFI 102 CCTGG CC^[ACGT]GG
SexAI 101 ACCTGGT A^CC[AT]GGT
SmlI 299 CTCAAG C^T[CT][GA]AG
SspBI 32 TGTACA T^GTACA
StuI 114 AGGCCT AGG^CCT
StyI 291 CCAAGG C^C[AT][AT]GG
TaqI 184 TCGA T^CGA
TfiI 510 GATTC G^A[AT]TC
Tsp509I 574 AATT ^AATT
TthHB8I 184 TCGA T^CGA
XhoII 295 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 11 TCCGGA T^CCGGA
266 TCCGGA
AfaI 33 GTAC GT^AC
454 GTAC
BsaWI 11 TCCGGA [AT]^CCGG[AT]
266 TCCGGA
BseAI 11 TCCGGA T^CCGGA
266 TCCGGA
BsgI 83 CTGCAC GTGCAG(16/14)
367 CTGCAC
BspEI 11 TCCGGA T^CCGGA
266 TCCGGA
Cac8I 438 GCCAGC GC[ACGT]^[ACGT]GC
445 GCTCGC
EarI 330 CTCTTC CTCTTC(1/4)
377 CTCTTC
HaeIII 115 GGCC GG^CC
420 GGCC
HapII 12 CCGG C^CGG
267 CCGG
HpaII 12 CCGG C^CGG
267 CCGG
Ksp632I 330 CTCTTC CTCTTC(1/4)
377 CTCTTC
MaeII 176 ACGT A^CGT
495 ACGT
MroI 11 TCCGGA T^CCGGA
266 TCCGGA
MspI 12 CCGG C^CGG
267 CCGG
NlaIV 72 GGGACC GG[ACGT]^[ACGT]CC
278 GGAGCC
RsaI 33 GTAC GT^AC
454 GTAC
SfaNI 541 GATGC GCATC(5/9)
384 GCATC
SfcI 156 CTGCAG C^T[GA][CT]AG
514 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BbvI BsrI BstF5I DdeI
DpnII Fnu4HI FokI ItaI MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI SspI Tru9I TseI TspRI