################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 202 GTCGAC GT^[AC][GT]AC AfaI 50 GTAC GT^AC AgeI 198 ACCGGT A^CCGGT Alw44I 315 GTGCAC G^TGCAC ApaI 706 GGGCCC GGGCC^C ApaLI 315 GTGCAC G^TGCAC AspHI 315 GTGCAC G[AT]GC[AT]^C BamHI 160 GGATCC G^GATCC BanI 625 GGTGCC G^G[CT][GA]CC BanII 706 GGGCCC G[GA]GC[CT]^C BbvCI 150 CCTCAGC CCTCAGC(-5/-2) BcnI 915 CCGGG CC^[GC]GG BsaBI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 475 GGTCTC GGTCTC(1/5) BsaWI 198 ACCGGT [AT]^CCGG[AT] BsgI 253 GTGCAG GTGCAG(16/14) BsiEI 200 CGGTCG CG[GA][CT]^CG BsiHKAI 315 GTGCAC G[AT]GC[AT]^C BsmBI 862 GAGACG CGTCTC(1/5) BspMI 657 ACCTGC ACCTGC(4/8) BsrDI 240 CATTGC GCAATG(2/0) BsrFI 198 ACCGGT [GA]^CCGG[CT] BstF5I 867 GGATG GGATG(2/0) BstXI 416 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 160 GGATCC [GA]^GATC[CT] Cfr10I 198 ACCGGT [GA]^CCGG[CT] DraI 784 TTTAAA TTT^AAA EcoRI 769 GAATTC G^AATTC FauI 640 CCCGC CCCGC(4/6) FokI 867 GGATG GGATG(9/13) HindIII 95 AAGCTT A^AGCTT HphI 440 TCACC GGTGA(8/7) MamI 327 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 160 GGATCC [GA]^GATC[CT] MseI 785 TTAA T^TAA NciI 915 CCGGG CC^[GC]GG NspI 897 GCATGT [GA]CATG^[CT] PinAI 198 ACCGGT A^CCGGT PshAI 546 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 528 CAGCTG CAG^CTG RsaI 50 GTAC GT^AC SalI 202 GTCGAC G^TCGAC SfaNI 332 GCATC GCATC(5/9) SmlI 92 CTCAAG C^T[CT][GA]AG StuI 429 AGGCCT AGG^CCT TaqI 203 TCGA T^CGA Tru9I 785 TTAA T^TAA TthHB8I 203 TCGA T^CGA XcmI 267 CCATATCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 160 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 121 GAATTT [GA]^AATT[CT] 769 GAATTC AlwNI 296 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 364 CAGTGCCTG ApoI 121 GAATTT [GA]^AATT[CT] 769 GAATTC AvaII 40 GGACC G^G[AT]CC 655 GGACC BbvI 358 GCAGC GCAGC(8/12) 805 GCTGC BmyI 315 GTGCAC G[AGT]GC[ACT]^C 706 GGGCCC BpmI 887 CTGGAG CTGGAG(16/14) 947 CTGGAG BseRI 148 CTCCTC GAGGAG(10/8) 922 GAGGAG BsmAI 862 GAGAC GTCTC(1/5) 476 GTCTC Bsp1286I 315 GTGCAC G[AGT]GC[ACT]^C 706 GGGCCC Cac8I 526 GCCAGC GC[ACGT]^[ACGT]GC 628 GCCAGC DdeI 151 CTCAG C^T[ACGT]AG 235 CTCAG Fnu4HI 358 GCAGC GC^[ACGT]GC 805 GCTGC HapII 199 CCGG C^CGG 915 CCGG HincII 202 GTCGAC GT[CT]^[GA]AC 910 GTTGAC HindII 202 GTCGAC GT[CT]^[GA]AC 910 GTTGAC HpaII 199 CCGG C^CGG 915 CCGG ItaI 358 GCAGC GC^[ACGT]GC 805 GCTGC MaeII 777 ACGT A^CGT 908 ACGT MslI 63 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 831 CATGGAGGTG MspI 199 CCGG C^CGG 915 CCGG PflMI 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 363 CCAGTGCCTGG PstI 293 CTGCAG CTGCA^G 659 CTGCAG SfcI 293 CTGCAG C^T[GA][CT]AG 659 CTGCAG TseI 358 GCAGC G^C[AT]GC 805 GCTGC Van91I 167 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 363 CCAGTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AlwI BalI BbsI BpuAI BsaJI BsiYI BslI BsrI BssKI BstNI Cfr13I DpnII DsaI EaeI EcoRII EcoT14I HaeIII HinfI MaeIII MboI MboII MfeI MluNI MnlI MscI MspA1I MunI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI TfiI Tsp45I Tsp509I TspRI