Restriction map for
pFN21AE2550
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 498 GAATTT [GA]^AATT[CT]
AcyI 716 GGCGTC G[GA]^CG[CT]C
AfaI 145 GTAC GT^AC
ApaI 9 GGGCCC GGGCC^C
ApoI 498 GAATTT [GA]^AATT[CT]
BanI 1144 GGCACC G^G[CT][GA]CC
BglII 1116 AGATCT A^GATCT
BlpI 67 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
BsaHI 716 GGCGTC G[GA]^CG[CT]C
BsaI 253 GAGACC GGTCTC(1/5)
BsgI 121 GTGCAG GTGCAG(16/14)
BsmI 330 GCATTC GAATGC(1/-1)
BssSI 1027 CTCGTG CACGAG(-5/-1)
CelII 67 GCTGAGC GC^T[ACGT]AGC
CfoI 171 GCGC GCG^C
DraIII 877 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 620 CCATGG C^C[GA][CT]GG
EarI 415 CTCTTC CTCTTC(1/4)
EcoRV 578 GATATC GAT^ATC
EcoT22I 547 ATGCAT ATGCA^T
HgaI 717 GCGTC GACGC(5/10)
HhaI 171 GCGC GCG^C
Hin1I 716 GGCGTC G[GA]^CG[CT]C
HinP1I 171 GCGC G^CGC
Ksp632I 415 CTCTTC CTCTTC(1/4)
MaeII 491 ACGT A^CGT
NcoI 620 CCATGG C^CATGG
NsiI 547 ATGCAT ATGCA^T
PflMI 426 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 912 GAGTC GAGTC(4/5)
PvuII 1007 CAGCTG CAG^CTG
RsaI 145 GTAC GT^AC
SapI 414 GCTCTTC GCTCTTC(1/4)
SphI 549 GCATGC GCATG^C
StuI 644 AGGCCT AGG^CCT
TfiI 864 GAATC G^A[AT]TC
Tsp509I 499 AATT ^AATT
Van91I 426 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 636 CCGC CCGC(-3/-1)
860 GCGG
AlwNI 1039 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1102 CAGCCTCTG
AocI 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
BalI 482 TGGCCA TGG^CCA
803 TGGCCA
BcnI 12 CCCGG CC^[GC]GG
55 CCGGG
BseRI 603 GAGGAG GAGGAG(10/8)
826 GAGGAG
BspMI 841 ACCTGC ACCTGC(4/8)
1038 GCAGGT
Bsu36I 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
DraII 281 AGGACCT [GA]G^G[ACGT]CC[CT]
1040 AGGTCCT
EaeI 482 TGGCCA [CT]^GGCC[GA]
803 TGGCCA
Eco81I 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
EcoO109I 281 AGGACCT [GA]G^G[ACGT]CC[CT]
1040 AGGTCCT
FauI 635 CCCGC CCCGC(4/6)
860 GCGGG
HinfI 864 GAATC G^A[ACGT]TC
912 GAGTC
MluNI 482 TGGCCA TGG^CCA
803 TGGCCA
MscI 482 TGGCCA TGG^CCA
803 TGGCCA
MspA1I 858 CAGCGG C[AC]G^C[GT]G
1007 CAGCTG
NciI 12 CCCGG CC^[GC]GG
55 CCGGG
NspI 549 GCATGC [GA]CATG^[CT]
670 ACATGC
PpuMI 281 AGGACCT [GA]G^G[AT]CC[CT]
1040 AGGTCCT
SbfI 561 CCTGCAGG CCTGCA^GG
1035 CCTGCAGG
Sse8387I 561 CCTGCAGG CCTGCA^GG
1035 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AluI AlwI AspHI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PstI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI XhoII