(Circular) (Six-base) MAPSORT of: pFN21AE2550.seq Check: 3758 from: 1 to: 5854 pFN21AE2550 5854 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2855 2855 Size: 5854 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3664 278 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3197 1937 Size: 1260 4594 Acc65I G'GTAC_C Cuts at: 3175 3175 Size: 5854 AclI AA'CG_TT Cuts at: 1563 3981 4354 1563 Size: 2418 373 3063 Fragments arranged by size: 3063 2418 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 3917 5301 5569 1632 Size: 1265 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1265 1020 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5657 AflIII A'CryG_T Cuts at: 1203 4753 5730 1203 Size: 3550 977 1327 Fragments arranged by size: 3550 1327 977 AgeI A'CCGG_T Cuts at: 4685 4685 Size: 5854 AhdI GACnn_n'nnGTC Cuts at: 4583 4583 Size: 5854 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5854 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2825 2857 2825 Size: 32 5822 AlwNI CAG_nnn'CTG Cuts at: 3049 3112 3597 5169 3049 Size: 63 485 1572 3734 Fragments arranged by size: 3734 1572 485 63 ApaI G_GGCC'C Cuts at: 2018 2018 Size: 5854 ApaLI G'TGCA_C Cuts at: 3911 5067 3911 Size: 1156 4698 ApoI r'AATT_y Cuts at: 1493 2503 3163 3389 3541 5650 1493 Size: 1010 660 226 152 2109 1697 Fragments arranged by size: 2109 1697 1010 660 226 152 AseI AT'TA_AT Cuts at: 160 1032 4408 160 Size: 872 3376 1606 Fragments arranged by size: 3376 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 AvaI C'yCGr_G Cuts at: 1870 1943 3179 1870 Size: 73 1236 4545 Fragments arranged by size: 4545 1236 73 BamHI G'GATC_C Cuts at: 3184 3551 3184 Size: 367 5487 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3149 3175 4630 618 Size: 325 217 627 1362 26 1455 1842 Fragments arranged by size: 1842 1455 1362 627 325 217 26 BanII G_rGCy'C Cuts at: 1391 2018 2079 2393 2421 2479 3033 3173 1391 Size: 627 61 314 28 58 554 140 4072 Fragments arranged by size: 4072 627 554 314 140 61 58 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2014 2078 2379 2534 928 Size: 971 115 64 301 155 4248 Fragments arranged by size: 4248 971 301 155 115 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4045 4079 1903 Size: 34 2108 34 3678 Fragments arranged by size: 3678 2108 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3746 4956 3746 Size: 1210 4644 BclI T'GATC_A Cuts at: 1548 1548 Size: 5854 BfrBI ATG'CAT Cuts at: 2554 2554 Size: 5854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4465 136 Size: 107 122 71 631 605 2793 1525 Fragments arranged by size: 2793 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3121 5849 3121 Size: 2728 3126 BlpI GC'TnA_GC Cuts at: 2073 3271 2073 Size: 1198 4656 Bme1580I G_kGCm'C Cuts at: 1790 2018 2622 2892 3152 3915 5071 1790 Size: 228 604 270 260 763 1156 2573 Fragments arranged by size: 2573 1156 763 604 270 260 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3056 3585 Size: 416 53 83 399 24 1041 598 529 Cuts at: 3585 4543 442 Size: 958 1753 Fragments arranged by size: 1753 1041 958 598 529 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2455 2459 4514 1239 Size: 1088 128 4 2055 2579 Fragments arranged by size: 2579 2055 1088 128 4 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3979 4844 5142 3979 Size: 865 298 4691 Fragments arranged by size: 4691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2252 4517 882 Size: 617 753 2265 2219 Fragments arranged by size: 2265 2219 753 617 BsaAI yAC'GTr Cuts at: 493 1204 3645 493 Size: 711 2441 2702 Fragments arranged by size: 2702 2441 711 BsaBI GATnn'nnATC Cuts at: 5848 5848 Size: 5854 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2722 3661 4043 4678 Size: 53 83 186 936 1189 939 382 635 Cuts at: 4678 275 Size: 1451 Fragments arranged by size: 1451 1189 939 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4685 4959 5106 5517 4287 Size: 398 274 147 411 4624 Fragments arranged by size: 4624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2825 2855 1178 Size: 30 165 30 1422 30 4177 Fragments arranged by size: 4177 1422 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2623 2846 1186 Size: 1437 223 4194 Fragments arranged by size: 4194 1437 223 BseYI C'CCAG_C Cuts at: 2011 2394 2754 5057 5553 2011 Size: 383 360 2303 496 2312 Fragments arranged by size: 2312 2303 496 383 360 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 1163 Size: 573 411 4870 Fragments arranged by size: 4870 573 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3304 4065 5093 1540 Size: 275 186 1303 761 1028 2301 Fragments arranged by size: 2301 1303 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2393 2421 2479 3033 3915 4000 5071 2393 Size: 28 58 554 882 85 1071 3176 Fragments arranged by size: 3176 1071 882 554 85 58 28 BsmI GAATG_Cn' Cuts at: 2335 3365 3458 2335 Size: 1030 93 4731 Fragments arranged by size: 4731 1030 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2079 2393 2421 2479 2622 2892 Size: 399 228 61 314 28 58 143 270 Cuts at: 2892 3033 3152 3173 3915 4000 5071 1391 Size: 141 119 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 314 270 228 143 141 119 85 61 58 28 21 BspEI T'CCGG_A Cuts at: 5517 5517 Size: 5854 BspHI T'CATG_A Cuts at: 3743 5473 3743 Size: 1730 4124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 3034 3209 844 Size: 2011 179 175 3489 Fragments arranged by size: 3489 2011 179 175 BsrBI CCG'CTC Cuts at: 1394 1823 3741 1394 Size: 429 1918 3507 Fragments arranged by size: 3507 1918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4349 4523 65 Size: 1183 3101 174 1396 Fragments arranged by size: 3101 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4498 4685 1700 Size: 110 2688 187 2869 Fragments arranged by size: 2869 2688 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5854 BssSI C'ACGA_G Cuts at: 3032 3914 4926 3032 Size: 882 1012 3960 Fragments arranged by size: 3960 1012 882 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5854 BstBI TT'CG_AA Cuts at: 4658 4658 Size: 5854 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5854 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5854 BstYI r'GATC_y Cuts at: 1972 2120 2364 3121 3184 3551 3936 3953 5405 Size: 148 244 757 63 367 385 17 1452 Cuts at: 5405 5491 5503 5849 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 757 385 367 346 244 148 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2291 2606 2291 Size: 315 5539 BtgI C'CryG_G Cuts at: 513 1065 2004 2625 513 Size: 552 939 621 3742 Fragments arranged by size: 3742 939 621 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5115 Fragments arranged by size: 5115 683 56 BtsI GCAGTG_nn' Cuts at: 2765 3370 4164 4184 2765 Size: 605 794 20 4435 Fragments arranged by size: 4435 794 605 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5819 DraI TTT'AAA Cuts at: 3158 3158 Size: 5854 DraIII CAC_nnn'GTG Cuts at: 2887 2887 Size: 5854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4861 809 Size: 507 3545 1802 Fragments arranged by size: 3545 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2487 2808 3301 Size: 54 1425 50 275 18 657 321 493 Cuts at: 3301 4190 5819 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 657 493 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3301 1537 Size: 275 1489 4090 Fragments arranged by size: 4090 1489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2426 3784 1353 Size: 1073 1358 3423 Fragments arranged by size: 3423 1358 1073 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4441 4815 4961 387 Size: 1064 138 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2391 2419 2477 3031 2391 Size: 28 58 554 5214 Fragments arranged by size: 5214 554 58 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2455 2459 2897 3917 4514 5301 Size: 393 695 128 4 438 1020 597 787 Cuts at: 5301 5569 1239 Size: 268 1524 Fragments arranged by size: 1524 1020 787 695 597 438 393 268 128 4 EcoO109I rG'GnC_Cy Cuts at: 2287 3046 2287 Size: 759 5095 EcoRI G'AATT_C Cuts at: 3163 3541 3163 Size: 378 5476 EcoRV GAT'ATC Cuts at: 2585 2585 Size: 5854 FspI TGC'GCA Cuts at: 4360 4360 Size: 5854 HaeII r_GCGC'y Cuts at: 1678 5001 1678 Size: 3323 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2290 2322 2527 2559 2707 Size: 32 135 32 718 32 205 32 148 Cuts at: 2707 2720 2739 2752 3106 3113 3138 3145 4495 Size: 13 19 13 354 7 25 7 1350 Cuts at: 4495 4527 4569 4601 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 718 354 205 148 135 42 32 32 32 32 32 32 25 19 13 13 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3198 3444 669 Size: 988 281 1260 246 3079 Fragments arranged by size: 3079 1260 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5854 HpaI GTT'AAC Cuts at: 3444 3444 Size: 5854 KpnI G_GTAC'C Cuts at: 3179 3179 Size: 5854 MfeI C'AATT_G Cuts at: 3453 3453 Size: 5854 MluI A'CGCG_T Cuts at: 5730 5730 Size: 5854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4968 5152 1305 Size: 3663 184 2007 Fragments arranged by size: 3663 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 2810 10 Size: 54 1425 343 657 321 3054 Fragments arranged by size: 3054 1425 657 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2669 3084 3812 4171 4330 518 Size: 648 991 512 415 728 359 159 2042 Fragments arranged by size: 2042 991 728 648 512 415 359 159 MspA1I CmG'CkG Cuts at: 1606 2865 3014 3270 3947 5095 5340 1606 Size: 1259 149 256 677 1148 245 2120 Fragments arranged by size: 2120 1259 1148 677 256 245 149 NcoI C'CATG_G Cuts at: 513 1065 2004 2625 513 Size: 552 939 621 3742 Fragments arranged by size: 3742 939 621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5854 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5854 NotI GC'GGCC_GC Cuts at: 3301 3301 Size: 5854 NsiI A_TGCA'T Cuts at: 2556 2556 Size: 5854 NspI r_CATG'y Cuts at: 1132 1229 2558 2679 3212 4757 1132 Size: 97 1329 121 533 1545 2229 Fragments arranged by size: 2229 1545 1329 533 121 97 PasI CC'CwG_GG Cuts at: 1348 2209 1348 Size: 861 4993 PciI A'CATG_T Cuts at: 4753 4753 Size: 5854 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 1108 Size: 1329 4525 PfoI T'CCnGG_A Cuts at: 2059 2343 3048 2059 Size: 284 705 4865 Fragments arranged by size: 4865 705 284 PmeI GTTT'AAAC Cuts at: 3158 3158 Size: 5854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 3923 3955 5436 5468 2825 Size: 32 1066 32 1481 32 3211 Fragments arranged by size: 3211 1481 1066 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 3046 2287 Size: 759 5095 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5854 PsiI TTA'TAA Cuts at: 2094 3424 2094 Size: 1330 4524 PspOMI G'GGCC_C Cuts at: 2014 2014 Size: 5854 PstI C_TGCA'G Cuts at: 830 2571 3019 3045 3206 830 Size: 1741 448 26 161 3478 Fragments arranged by size: 3478 1741 448 161 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5854 PvuII CAG'CTG Cuts at: 3014 3014 Size: 5854 SacI G_AGCT'C Cuts at: 2393 2421 2479 3033 2393 Size: 28 58 554 5214 Fragments arranged by size: 5214 554 58 28 SalI G'TCGA_C Cuts at: 1936 3196 1936 Size: 1260 4594 SapI GCTCTTCn'nnn_ Cuts at: 2426 2426 Size: 5854 SbfI CC_TGCA'GG Cuts at: 2571 3045 3206 2571 Size: 474 161 5219 Fragments arranged by size: 5219 474 161 ScaI AGT'ACT Cuts at: 4102 5814 4102 Size: 1712 4142 SfcI C'TryA_G Cuts at: 826 1045 2567 3015 3041 3202 4337 5018 5209 Size: 219 1522 448 26 161 1135 681 191 Cuts at: 5209 826 Size: 1471 Fragments arranged by size: 1522 1471 1135 681 448 219 191 161 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5854 SmaI CCC'GGG Cuts at: 1872 3181 1872 Size: 1309 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3958 4859 5121 820 Size: 197 926 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3645 493 Size: 3152 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5854 SphI G_CATG'C Cuts at: 1132 2558 3212 1132 Size: 1426 654 3774 Fragments arranged by size: 3774 1426 654 SspI AAT'ATT Cuts at: 5 52 3778 5 Size: 47 3726 2081 Fragments arranged by size: 3726 2081 47 StuI AGG'CCT Cuts at: 1517 2651 1517 Size: 1134 4720 StyI C'CwwG_G Cuts at: 513 1065 2004 2484 2625 2824 3293 513 Size: 552 939 480 141 199 469 3074 Fragments arranged by size: 3074 939 552 480 469 199 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 2794 3075 3881 3898 4051 4236 Size: 1255 1188 26 281 806 17 153 185 Cuts at: 4236 4681 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1188 806 445 281 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4100 5812 96 Size: 274 80 33 51 1443 2123 1712 138 Fragments arranged by size: 2123 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5814 5846 5814 Size: 32 5822 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5854 XbaI T'CTAG_A Cuts at: 3190 3190 Size: 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5854 XmaI C'CCGG_G Cuts at: 1870 3179 1870 Size: 1309 4545 XmnI GAAnn'nnTTC Cuts at: 3983 3983 Size: 5854 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3662 276 Size: 53 83 186 936 2128 2468 Fragments arranged by size: 2468 2128 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI