################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tsp509I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### ApaI 453 GGGCCC GGGCC^C AspI 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1115 TGGCCA TGG^CCA BamHI 989 GGATCC G^GATCC BanI 1087 GGCACC G^G[CT][GA]CC BspHI 917 TCATGA T^CATGA BstEII 694 GGTTACC G^GT[ACGT]ACC BstPI 694 GGTTACC G^GT[ACGT]ACC BstXI 9 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 495 GCGC GCG^C EcoO65I 694 GGTTACC G^GT[ACGT]ACC FauI 871 GCGGG CCCGC(4/6) HhaI 495 GCGC GCG^C HinP1I 495 GCGC G^CGC HphI 155 TCACC GGTGA(8/7) MluNI 1115 TGGCCA TGG^CCA MscI 1115 TGGCCA TGG^CCA MseI 326 TTAA T^TAA PflFI 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 368 AGGTCCT [GA]G^G[AT]CC[CT] PstI 649 CTGCAG CTGCA^G RcaI 917 TCATGA T^CATGA SacI 288 GAGCTC GAGCT^C SapI 234 GCTCTTC GCTCTTC(1/4) SbfI 648 CCTGCAGG CCTGCA^GG SfaNI 830 GCATC GCATC(5/9) Sse8387I 648 CCTGCAGG CCTGCA^GG Tru9I 326 TTAA T^TAA Tth111I 933 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 428 CCAGCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AvaII 369 GGTCC G^G[AT]CC 597 GGACC BcnI 855 CCGGG CC^[GC]GG 954 CCGGG BglI 818 GCCTTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1216 GCCAGCACGGC BpmI 285 CTGGAG CTGGAG(16/14) 443 CTGGAG BseRI 1020 CTCCTC GAGGAG(10/8) 637 GAGGAG BsmAI 535 GTCTC GTCTC(1/5) 548 GTCTC BspMI 570 ACCTGC ACCTGC(4/8) 721 ACCTGC BsrDI 1035 CATTGC GCAATG(2/0) 1158 CATTGC BstAPI 172 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 651 GCAGGAGCTGC BstYI 122 AGATCC [GA]^GATC[CT] 989 GGATCC DraIII 405 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 521 CACTGTGTG DsaI 860 CCATGG C^C[GA][CT]GG 900 CCATGG EaeI 1115 TGGCCA [CT]^GGCC[GA] 1214 CGGCCA EcoNI 333 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 750 CCTTATCCAGG EcoT14I 860 CCATGG C^C[AT][AT]GG 900 CCATGG HapII 855 CCGG C^CGG 954 CCGG HincII 268 GTCAAC GT[CT]^[GA]AC 423 GTCAAC HindII 268 GTCAAC GT[CT]^[GA]AC 423 GTCAAC HpaII 855 CCGG C^CGG 954 CCGG MflI 122 AGATCC [GA]^GATC[CT] 989 GGATCC MslI 930 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1254 CACCAAGATG MspA1I 489 CAGCTG C[AC]G^C[GT]G 836 CAGCTG MspI 855 CCGG C^CGG 954 CCGG NciI 855 CCGGG CC^[GC]GG 954 CCGGG NcoI 860 CCATGG C^CATGG 900 CCATGG PflMI 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 980 CCAAGCTCTGG PvuII 489 CAGCTG CAG^CTG 836 CAGCTG StyI 860 CCATGG C^C[AT][AT]GG 900 CCATGG Tsp45I 964 GTGAC ^GT[GC]AC 971 GTGAC Van91I 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 980 CCAAGCTCTGG XhoII 122 AGATCC [GA]^GATC[CT] 989 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI TseI TspRI