(Circular) (Six-base) MAPSORT of: pFN21AE4415.seq Check: 3118 from: 1 to: 5992 pFN21AE4415 5992 bp With 182 enzymes: SgfI * March 28, 2012 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3139 3225 4055 5439 5707 1632 Size: 1507 86 830 1384 268 1917 Fragments arranged by size: 1917 1507 1384 830 268 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2306 2350 2662 3735 5307 2306 Size: 44 312 1073 1572 2991 Fragments arranged by size: 2991 1572 1073 312 44 ApaI G_GGCC'C Cuts at: 2462 2462 Size: 5992 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 2994 3322 3689 2994 Size: 328 367 5297 Fragments arranged by size: 5297 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3092 3313 4768 618 Size: 325 217 627 1305 221 1455 1842 Fragments arranged by size: 1842 1455 1305 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2297 2462 2633 3311 1391 Size: 906 165 171 678 4072 Fragments arranged by size: 4072 906 678 171 165 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5021 BbvCI CC'TCA_GC Cuts at: 3288 3288 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2829 3227 4603 Size: 107 122 71 631 605 1157 398 1376 Cuts at: 4603 136 Size: 1525 Fragments arranged by size: 1525 1376 1157 631 605 398 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2462 2527 2809 3076 4053 5209 1790 Size: 672 65 282 267 977 1156 2573 Fragments arranged by size: 2573 1156 977 672 282 267 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2749 3723 4681 Size: 416 53 83 399 24 1332 974 958 Cuts at: 4681 442 Size: 1753 Fragments arranged by size: 1753 1332 974 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3093 3093 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 2469 4652 1239 Size: 1072 158 2183 2579 Fragments arranged by size: 2579 2183 1072 158 Bpu10I CC'TnA_GC Cuts at: 3288 3288 Size: 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4655 882 Size: 617 3156 2219 Fragments arranged by size: 3156 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4181 4816 275 Size: 53 83 186 936 2266 382 635 1451 Fragments arranged by size: 2266 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5767 Fragments arranged by size: 5767 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2657 3016 1186 Size: 1471 359 4162 Fragments arranged by size: 4162 1471 359 BseYI C'CCAG_C Cuts at: 2350 2455 3143 5195 5691 2350 Size: 105 688 2052 496 2651 Fragments arranged by size: 2651 2052 688 496 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2233 2297 2651 2691 3188 4053 4138 5209 2233 Size: 64 354 40 497 865 85 1071 3016 Fragments arranged by size: 3016 1071 865 497 354 85 64 40 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2297 2462 2527 2633 2651 2691 Size: 399 443 64 165 65 106 18 40 Cuts at: 2691 2809 3076 3188 3311 4053 4138 5209 1391 Size: 118 267 112 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 267 165 123 118 112 106 85 65 64 40 18 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 2922 3881 5611 2922 Size: 959 1730 3303 Fragments arranged by size: 3303 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2584 2735 3347 844 Size: 1740 151 612 3489 Fragments arranged by size: 3489 1740 612 151 BsrBI CCG'CTC Cuts at: 1394 1823 3879 1394 Size: 429 2056 3507 Fragments arranged by size: 3507 2056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3039 3162 4487 4661 65 Size: 1183 1791 123 1325 174 1396 Fragments arranged by size: 1791 1396 1325 1183 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 2183 2662 794 Size: 1389 479 4124 Fragments arranged by size: 4124 1389 479 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 2699 1184 Size: 1515 4477 BstXI CCAn_nnnn'nTGG Cuts at: 2021 2021 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2127 2994 3322 3689 4074 4091 5543 5629 Size: 155 867 328 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 867 385 367 346 328 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2865 2905 513 Size: 552 939 861 40 3600 Fragments arranged by size: 3600 939 861 552 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2821 487 Size: 683 56 1595 3658 Fragments arranged by size: 3658 1595 683 56 BtsI GCAGTG_nn' Cuts at: 3508 4302 4322 3508 Size: 794 20 5178 Fragments arranged by size: 5178 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3070 3105 529 Size: 35 2506 35 3416 Fragments arranged by size: 3416 2506 35 35 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DraIII CAC_nnn'GTG Cuts at: 2415 2531 2415 Size: 116 5876 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3120 3219 3439 Size: 54 1425 50 275 18 1290 99 220 Cuts at: 3439 4328 5957 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1290 889 275 220 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2246 2251 2960 3922 1353 Size: 765 128 5 709 962 3423 Fragments arranged by size: 3423 962 765 709 128 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2295 2295 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2469 3139 3225 4055 4652 5439 Size: 393 679 158 670 86 830 597 787 Cuts at: 5439 5707 1239 Size: 268 1524 Fragments arranged by size: 1524 830 787 679 670 597 393 268 158 86 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2759 2342 Size: 417 5575 EcoO109I rG'GnC_Cy Cuts at: 2374 2459 2821 2374 Size: 85 362 5545 Fragments arranged by size: 5545 362 85 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2650 2650 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2215 2247 4633 4665 4707 Size: 32 135 32 643 32 2386 32 42 Cuts at: 4707 4739 1373 Size: 32 2626 Fragments arranged by size: 2626 2386 643 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2430 3336 3582 669 Size: 988 281 337 155 906 246 3079 Fragments arranged by size: 3079 988 906 337 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2566 2910 3161 5106 5290 1305 Size: 1261 344 251 1945 184 2007 Fragments arranged by size: 2007 1945 1261 344 251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3122 10 Size: 54 1425 343 1290 2880 Fragments arranged by size: 2880 1425 1290 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3263 3950 4309 4468 518 Size: 648 1773 324 687 359 159 2042 Fragments arranged by size: 2042 1773 687 648 359 324 159 MspA1I CmG'CkG Cuts at: 1606 2496 2843 3408 4085 5233 5478 1606 Size: 890 347 565 677 1148 245 2120 Fragments arranged by size: 2120 1148 890 677 565 347 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2865 2905 513 Size: 552 939 861 40 3600 Fragments arranged by size: 3600 939 861 552 40 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 3350 4895 1132 Size: 97 2121 1545 2229 Fragments arranged by size: 2229 2121 1545 97 PasI CC'CwG_GG Cuts at: 1348 2014 2344 1348 Size: 666 330 4996 Fragments arranged by size: 4996 666 330 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2991 1108 Size: 1242 641 4109 Fragments arranged by size: 4109 1242 641 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2374 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2458 2458 Size: 5992 PstI C_TGCA'G Cuts at: 830 2658 3344 830 Size: 1828 686 3478 Fragments arranged by size: 3478 1828 686 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2496 2843 2496 Size: 347 5645 SacI G_AGCT'C Cuts at: 2297 2297 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 2246 2246 Size: 5992 SbfI CC_TGCA'GG Cuts at: 2658 3344 2658 Size: 686 5306 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SfcI C'TryA_G Cuts at: 826 1045 2065 2491 2654 3175 3340 4475 5156 Size: 219 1020 426 163 521 165 1135 681 Cuts at: 5156 5347 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1020 681 521 426 219 191 165 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4096 4997 5259 820 Size: 197 926 2153 901 262 1553 Fragments arranged by size: 2153 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2004 2865 2905 3431 513 Size: 552 939 861 40 526 3074 Fragments arranged by size: 3074 939 861 552 526 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2674 3187 4019 4036 4189 4374 4819 Size: 1255 1094 513 832 17 153 185 445 Cuts at: 4819 325 Size: 1498 Fragments arranged by size: 1498 1255 1094 832 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2732 4238 5950 Size: 274 80 33 51 1443 755 1506 1712 Cuts at: 5950 96 Size: 138 Fragments arranged by size: 1712 1506 1443 755 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 2941 1316 Size: 1625 4367 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2440 2440 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI