(Circular) (Six-base) MAPSORT of: pFN21AE4450.seq Check: 7599 from: 1 to: 6109 pFN21AE4450 6109 bp With 182 enzymes: SgfI * March 28, 2012 13:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2809 2809 Size: 6109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3452 1937 Size: 487 1028 4594 Fragments arranged by size: 4594 1028 487 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 4172 5556 5824 1632 Size: 1089 1451 1384 268 1917 Fragments arranged by size: 1917 1451 1384 1089 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 2921 3852 5424 2921 Size: 931 1572 3606 Fragments arranged by size: 3606 1572 931 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 6109 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 3434 1870 Size: 73 1491 4545 Fragments arranged by size: 4545 1491 73 BamHI G'GATC_C Cuts at: 2888 3439 3806 2888 Size: 551 367 5191 Fragments arranged by size: 5191 551 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 3430 4885 618 Size: 325 217 627 223 1420 1455 1842 Fragments arranged by size: 1842 1455 1420 627 325 223 217 BanII G_rGCy'C Cuts at: 1391 2283 2856 3428 1391 Size: 892 573 572 4072 Fragments arranged by size: 4072 892 573 572 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3190 3377 928 Size: 971 1291 187 3660 Fragments arranged by size: 3660 1291 971 187 BbvCI CC'TCA_GC Cuts at: 2768 3146 2768 Size: 378 5731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2386 6104 2386 Size: 3718 2391 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 2013 2283 4170 5326 1790 Size: 223 270 1887 1156 2573 Fragments arranged by size: 2573 1887 1156 270 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 2681 3840 Size: 416 53 83 399 24 1240 24 1159 Cuts at: 3840 4798 442 Size: 958 1753 Fragments arranged by size: 1753 1240 1159 958 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2437 4769 1239 Size: 1198 2332 2579 Fragments arranged by size: 2579 2332 1198 Bpu10I CC'TnA_GC Cuts at: 2768 3146 2768 Size: 378 5731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4234 5099 5397 4234 Size: 865 298 4946 Fragments arranged by size: 4946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4772 882 Size: 617 3273 2219 Fragments arranged by size: 3273 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4542 4940 5214 5361 5772 4542 Size: 398 274 147 411 4879 Fragments arranged by size: 4879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5884 Fragments arranged by size: 5884 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6109 BseYI C'CCAG_C Cuts at: 2106 2181 3176 5312 5808 2106 Size: 75 995 2136 496 2407 Fragments arranged by size: 2407 2136 995 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 1163 Size: 573 1051 4485 Fragments arranged by size: 4485 1051 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2296 3559 4320 5348 1540 Size: 275 186 295 1263 761 1028 2301 Fragments arranged by size: 2301 1263 1028 761 295 275 186 BsiHKAI G_wGCw'C Cuts at: 2856 4170 4255 5326 2856 Size: 1314 85 1071 3639 Fragments arranged by size: 3639 1314 1071 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2283 2856 3428 4170 4255 5326 Size: 399 223 270 573 572 742 85 1071 Cuts at: 5326 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 573 572 399 270 223 85 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2809 2906 3053 3464 844 Size: 1965 97 147 411 3489 Fragments arranged by size: 3489 1965 411 147 97 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 4604 4778 65 Size: 1183 2043 1313 174 1396 Fragments arranged by size: 2043 1396 1313 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3374 4753 4940 1700 Size: 110 1564 1379 187 2869 Fragments arranged by size: 2869 1564 1379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2386 2888 3439 3806 4191 4208 5660 5746 Size: 414 502 551 367 385 17 1452 86 Cuts at: 5746 5758 6104 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 551 502 414 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2425 2425 Size: 6109 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2686 3625 4419 4439 2100 Size: 586 939 794 20 3770 Fragments arranged by size: 3770 939 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DraIII CAC_nnn'GTG Cuts at: 2035 2035 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3556 4445 6074 Size: 54 1425 50 275 18 1726 889 1629 Cuts at: 6074 8 Size: 43 Fragments arranged by size: 1726 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4039 1353 Size: 2686 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2721 4172 4769 5556 5824 1239 Size: 393 805 284 1451 597 787 268 1524 Fragments arranged by size: 1524 1451 805 787 597 393 284 268 EcoNI CCTnn'n_nnAGG Cuts at: 3112 3112 Size: 6109 EcoO109I rG'GnC_Cy Cuts at: 2279 2598 2636 2279 Size: 319 38 5752 Fragments arranged by size: 5752 319 38 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 5256 1678 Size: 3578 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 2590 2622 3014 Size: 32 135 32 961 32 25 32 392 Cuts at: 3014 3046 4750 4782 4824 4856 1373 Size: 32 1704 32 42 32 2626 Fragments arranged by size: 2626 1704 961 392 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 5223 5407 1305 Size: 1185 2733 184 2007 Fragments arranged by size: 2733 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 3389 4067 4426 4585 518 Size: 648 1707 516 678 359 159 2042 Fragments arranged by size: 2042 1707 678 648 516 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2292 2581 3525 4202 5350 5595 1606 Size: 596 90 289 944 677 1148 245 2120 Fragments arranged by size: 2120 1148 944 677 596 289 245 90 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3354 3467 5012 1132 Size: 97 2125 113 1545 2229 Fragments arranged by size: 2229 2125 1545 113 97 PasI CC'CwG_GG Cuts at: 1348 2276 3130 1348 Size: 928 854 4327 Fragments arranged by size: 4327 928 854 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 3078 1108 Size: 1970 4139 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2598 2636 2598 Size: 38 6071 PshAI GACnn'nnGTC Cuts at: 1613 2939 3011 1613 Size: 1326 72 4711 Fragments arranged by size: 4711 1326 72 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 6109 PstI C_TGCA'G Cuts at: 830 2580 3461 830 Size: 1750 881 3478 Fragments arranged by size: 3478 1750 881 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2202 2581 2202 Size: 379 5730 SacI G_AGCT'C Cuts at: 2856 2856 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 2810 4357 6069 2810 Size: 1547 1712 2850 Fragments arranged by size: 2850 1712 1547 SfcI C'TryA_G Cuts at: 826 1045 2197 2576 3457 4592 5273 5464 826 Size: 219 1152 379 881 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 881 681 379 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4213 5114 5376 820 Size: 197 926 2270 901 262 1553 Fragments arranged by size: 2270 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2004 3548 513 Size: 552 939 1544 3074 Fragments arranged by size: 3074 1544 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 4136 4153 4306 4491 4936 325 Size: 1255 777 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 4355 6067 Size: 274 80 33 51 1443 831 1547 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 1712 1547 1443 831 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6069 6101 6069 Size: 32 6077 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 4738 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 2189 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI