(Linear) (Six-base) MAPSORT of: KIEE5253.seq Check: 6474 from: 1 to: 597 KIEE5253.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AatII G_ACGT'C Cuts at: 0 380 597 Size: 380 217 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 597 Size: 52 545 AleI CACnn'nnGTG Cuts at: 0 535 597 Size: 535 62 AlwNI CAG_nnn'CTG Cuts at: 0 226 573 597 Size: 226 347 24 Fragments arranged by size: 347 226 24 ApoI r'AATT_y Cuts at: 0 189 349 597 Size: 189 160 248 Fragments arranged by size: 248 189 160 BbsI GAAGACnn'nnnn_ Cuts at: 0 316 404 597 Size: 316 88 193 Fragments arranged by size: 316 193 88 BciVI GTATCCnnnnn_n' Cuts at: 0 140 597 Size: 140 457 BglI GCCn_nnn'nGGC Cuts at: 0 97 518 597 Size: 97 421 79 Fragments arranged by size: 421 97 79 Bme1580I G_kGCm'C Cuts at: 0 532 597 Size: 532 65 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 442 597 Size: 343 99 155 Fragments arranged by size: 343 155 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 597 Size: 216 381 BsaHI Gr'CG_yC Cuts at: 0 377 597 Size: 377 220 BsiHKAI G_wGCw'C Cuts at: 0 359 597 Size: 359 238 Bsp1286I G_dGCh'C Cuts at: 0 359 532 597 Size: 359 173 65 Fragments arranged by size: 359 173 65 BsrFI r'CCGG_y Cuts at: 0 517 597 Size: 517 80 BssSI C'ACGA_G Cuts at: 0 531 597 Size: 531 66 BstAPI GCAn_nnn'nTGC Cuts at: 0 226 597 Size: 226 371 BstEII G'GTnAC_C Cuts at: 0 261 597 Size: 261 336 BstXI CCAn_nnnn'nTGG Cuts at: 0 273 321 597 Size: 273 48 276 Fragments arranged by size: 276 273 48 BtgI C'CryG_G Cuts at: 0 524 597 Size: 524 73 BtsI GCAGTG_nn' Cuts at: 0 227 597 Size: 227 370 EaeI y'GGCC_r Cuts at: 0 41 597 Size: 41 556 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 157 562 597 Size: 157 405 35 Fragments arranged by size: 405 157 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 343 442 597 Size: 52 291 99 155 Fragments arranged by size: 291 155 99 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 303 335 547 579 597 Size: 303 32 212 32 18 Fragments arranged by size: 303 212 32 32 18 MslI CAynn'nnrTG Cuts at: 0 535 597 Size: 535 62 PflMI CCAn_nnn'nTGG Cuts at: 0 465 597 Size: 465 132 PfoI T'CCnGG_A Cuts at: 0 458 597 Size: 458 139 SfiI GGCCn_nnn'nGGCC Cuts at: 0 97 597 Size: 97 500 SmlI C'TyrA_G Cuts at: 0 195 597 Size: 195 402 Tth111I GACn'n_nGTC Cuts at: 0 267 597 Size: 267 330 ZraI GAC'GTC Cuts at: 0 378 597 Size: 378 219 Enzymes that do cut: AatII AcuI AleI AlwNI ApoI BbsI BciVI BglI Bme1580I BpmI BpuEI BsaHI BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstEII BstXI BtgI BtsI EaeI EciI Eco57MI Hin4I MslI PflMI PfoI SfiI SmlI Tth111I ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI