##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
Fnu4HI      FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          402  CCGC                 CCGC(-3/-1)
BanI           90  GGCACC               G^G[CT][GA]CC
BciVI         223  GTATCC               GTATCC(6/5)
BclI          147  TGATCA               T^GATCA
BseRI         459  GAGGAG               GAGGAG(10/8)
BsiYI         124  CCCTTTGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          124  CCCTTTGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         312  GTCTC                GTCTC(1/5)
BspLU11I      176  ACATGT               A^CATGT
BssKI         236  CCAGG                ^CC[ACGT]GG
BstAPI         91  GCACCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         236  CCAGG                CC^[AT]GG
Cac8I         240  GCATGC               GC[ACGT]^[ACGT]GC
Cfr13I         18  GGCCC                G^G[ACGT]CC
DpnII         148  GATC                 ^GATC
EcoRII        236  CCAGG                ^CC[AT]GG
EcoT22I       188  ATGCAT               ATGCA^T
FauI          401  CCCGC                CCCGC(4/6)
FbaI          147  TGATCA               T^GATCA
HincII        284  GTCAAC               GT[CT]^[GA]AC
HindII        284  GTCAAC               GT[CT]^[GA]AC
MboI          148  GATC                 ^GATC
MseI          160  TTAA                 T^TAA
MvaI          236  CCAGG                CC^[AT]GG
NdeII         148  GATC                 ^GATC
NlaIV          90  GGCACC               GG[ACGT]^[ACGT]CC
NsiI          188  ATGCAT               ATGCA^T
SapI           54  GCTCTTC              GCTCTTC(1/4)
Sau3AI        148  GATC                 ^GATC
Sau96I         18  GGCCC                G^G[ACGT]CC
ScrFI         236  CCAGG                CC^[ACGT]GG
SfaNI         376  GATGC                GCATC(5/9)
SmlI          307  CTTGAG               C^T[CT][GA]AG
SphI          240  GCATGC               GCATG^C
SspI          431  AATATT               AAT^ATT
TfiI          475  GAATC                G^A[AT]TC
Tru9I         160  TTAA                 T^TAA
Tsp509I       444  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          303  GTAC                 GT^AC
              355  GTAC                
AflIII        176  ACATGT               A^C[GA][CT]GT
              327  ACGTGT              
AluI           23  AGCT                 AG^CT
              195  AGCT                
AspHI          31  GTGCTC               G[AT]GC[AT]^C
               52  GTGCTC              
BsiHKAI        31  GTGCTC               G[AT]GC[AT]^C
               52  GTGCTC              
BsrI          260  CCAGT                ACTGG(1/-1)
              358  CCAGT               
HaeIII         18  GGCC                 GG^CC
               77  GGCC                
HphI          112  GGTGA                GGTGA(8/7)
              257  TCACC               
MaeII         327  ACGT                 A^CGT
              366  ACGT                
NspI          176  ACATGT               [GA]CATG^[CT]
              240  GCATGC              
PleI           39  GACTC                GAGTC(4/5)
              310  GAGTC               
RsaI          303  GTAC                 GT^AC
              355  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

BbsI        BmyI        BpuAI       Bsp1286I    DdeI        
EarI        HinfI       Ksp632I     MaeIII      MboII       
MnlI        MslI        MwoI        NlaIII      Tsp45I      
TspRI