Restriction map for
pFN21AE2681
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SfiI SfoI SfuI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TaqI Tru9I TthHB8I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 608 GGTACC G^GTACC
AccI 779 GTCTAC GT^[AC][GT]AC
AcyI 776 GGCGTC G[GA]^CG[CT]C
AflIII 304 ACATGT A^C[GA][CT]GT
ApaI 387 GGGCCC GGGCC^C
Asp700I 201 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 608 GGTACC G^GTACC
AspHI 1046 GAGCTC G[AT]GC[AT]^C
AspI 868 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 890 GGATCC G^GATCC
BbsI 964 GAAGAC GAAGAC(2/6)
BciVI 881 GGATAC GTATCC(6/5)
BpmI 910 CTGGAG CTGGAG(16/14)
BpuAI 964 GAAGAC GAAGAC(2/6)
BsaHI 776 GGCGTC G[GA]^CG[CT]C
BseRI 124 GAGGAG GAGGAG(10/8)
BsiEI 101 CGGCCG CG[GA][CT]^CG
BsiHKAI 1046 GAGCTC G[AT]GC[AT]^C
BsmBI 1059 GAGACG CGTCTC(1/5)
BsmI 456 GAATGC GAATGC(1/-1)
BspLU11I 304 ACATGT A^CATGT
EagI 101 CGGCCG C^GGCCG
EclXI 101 CGGCCG C^GGCCG
Eco52I 101 CGGCCG C^GGCCG
EcoNI 276 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 777 GCGTC GACGC(5/10)
Hin1I 776 GGCGTC G[GA]^CG[CT]C
HindIII 589 AAGCTT A^AGCTT
KpnI 608 GGTACC GGTAC^C
KspI 115 CCGCGG CCGC^GG
MaeII 515 ACGT A^CGT
MaeIII 595 GTCAC ^GT[ACGT]AC
NaeI 650 GCCGGC GCC^GGC
NgoMIV 650 GCCGGC G^CCGGC
PflFI 868 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 168 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 473 GGGACCT [GA]G^G[AT]CC[CT]
PstI 478 CTGCAG CTGCA^G
SacI 1046 GAGCTC GAGCT^C
SacII 115 CCGCGG CCGC^GG
SbfI 477 CCTGCAGG CCTGCA^GG
SexAI 614 ACCAGGT A^CC[AT]GGT
SfcI 478 CTGCAG C^T[GA][CT]AG
SgrAI 153 CACCGGCG C[GA]^CCGG[CT]G
SmaI 249 CCCGGG CCC^GGG
Sse8387I 477 CCTGCAGG CCTGCA^GG
Tsp45I 595 GTCAC ^GT[GC]AC
Tsp509I 759 AATT ^AATT
Tth111I 868 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 168 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 249 CCCGGG C^CCGGG
XmaI 249 CCCGGG C^CCGGG
XmnI 201 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 609 GTAC GT^AC
681 GTAC
AlwNI 169 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
327 CAGAGTCTG
AvaI 249 CCCGGG C^[CT]CG[GA]G
1023 CTCGGG
BsaI 163 GAGACC GGTCTC(1/5)
664 GAGACC
BsgI 14 CTGCAC GTGCAG(16/14)
600 CTGCAC
BsmFI 473 GGGAC GGGAC(10/14)
554 GGGAC
BsoBI 249 CCCGGG C^[CT]CG[GA]G
1023 CTCGGG
BspMI 476 ACCTGC ACCTGC(4/8)
598 ACCTGC
BsrFI 154 ACCGGC [GA]^CCGG[CT]
650 GCCGGC
BstYI 53 GGATCT [GA]^GATC[CT]
890 GGATCC
Cfr10I 154 ACCGGC [GA]^CCGG[CT]
650 GCCGGC
DraII 387 GGGCCCT [GA]G^G[ACGT]CC[CT]
473 GGGACCT
EaeI 101 CGGCCG [CT]^GGCC[GA]
652 CGGCCA
EcoO109I 387 GGGCCCT [GA]G^G[ACGT]CC[CT]
473 GGGACCT
EcoT14I 995 CCTTGG C^C[AT][AT]GG
1041 CCAAGG
FauI 254 GCGGG CCCGC(4/6)
540 GCGGG
HaeII 136 AGCGCC [GA]GCGC^[CT]
647 AGCGCC
HphI 198 GGTGA GGTGA(8/7)
596 TCACC
MflI 53 GGATCT [GA]^GATC[CT]
890 GGATCC
NspI 263 GCATGT [GA]CATG^[CT]
304 ACATGT
PleI 329 GAGTC GAGTC(4/5)
872 GAGTC
PvuII 748 CAGCTG CAG^CTG
1004 CAGCTG
RsaI 609 GTAC GT^AC
681 GTAC
SmlI 876 CTTGAG C^T[CT][GA]AG
938 CTCAAG
StyI 995 CCTTGG C^C[AT][AT]GG
1041 CCAAGG
XhoII 53 GGATCT [GA]^GATC[CT]
890 GGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI TspRI