Restriction map for
pFN21AE2681
(Circular) (Six-base) MAPSORT of: pFN21AE2681.seq Check: 7636 from: 1 to: 5803
pFN21AE2681 5803 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2612 2612
Size: 5803
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2785 3146 1937
Size: 848 361 4594
Fragments arranged by size:
4594 848 361
Acc65I G'GTAC_C
Cuts at: 2613 3124 2613
Size: 511 5292
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2197 2819 3866 5250 5518 1632
Size: 431 134 622 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1047 622 431 268 134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 2309 4702 5679 1203
Size: 1106 2393 977 1327
Fragments arranged by size:
2393 1327 1106 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 2312 1166
Size: 1146 4657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 5771
AlwNI CAG_nnn'CTG
Cuts at: 2179 2337 3546 5118 2179
Size: 158 1209 1572 2864
Fragments arranged by size:
2864 1572 1209 158
ApaI G_GGCC'C
Cuts at: 2396 2396
Size: 5803
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 3028 3128 1870
Size: 73 311 774 100 4545
Fragments arranged by size:
4545 774 311 100 73
BamHI G'GATC_C
Cuts at: 2895 3133 3500 2895
Size: 238 367 5198
Fragments arranged by size:
5198 367 238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2156 2297 2613 2959
Size: 325 217 627 258 111 141 316 346
Cuts at: 2959 3124 4579 618
Size: 165 1455 1842
Fragments arranged by size:
1842 1455 627 346 325 316 258 217
165 141 111
BanII G_rGCy'C
Cuts at: 1391 2396 2492 2934 3055 3122 1391
Size: 1005 96 442 121 67 4072
Fragments arranged by size:
4072 1005 442 121 96 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2976 928
Size: 971 1077 3755
Fragments arranged by size:
3755 1077 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3695 4905 2880
Size: 815 1210 3778
Fragments arranged by size:
3778 1210 815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4414 136
Size: 107 122 71 631 605 337 2405 1525
Fragments arranged by size:
2405 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2396 2964 3864 5020 1790
Size: 369 237 568 900 1156 2573
Fragments arranged by size:
2573 1156 900 568 369 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 2798 3534
Size: 416 53 83 399 24 647 734 736
Cuts at: 3534 4492 442
Size: 958 1753
Fragments arranged by size:
1753 958 736 734 647 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 4463 1239
Size: 1697 1527 2579
Fragments arranged by size:
2579 1697 1527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 2928 3928 4793 5091 2902
Size: 26 1000 865 298 3614
Fragments arranged by size:
3614 1000 865 298 26
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 2663 4466 882
Size: 617 663 501 1803 2219
Fragments arranged by size:
2219 1803 663 617 501
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2782 3610 3992 4627
Size: 53 83 186 936 1249 828 382 635
Cuts at: 4627 275
Size: 1451
Fragments arranged by size:
1451 1249 936 828 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2562 2592 2695
Size: 30 165 30 852 30 277 30 103
Cuts at: 2695 2725 1178
Size: 30 4256
Fragments arranged by size:
4256 852 277 165 103 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 1186
Size: 958 4845
BseYI C'CCAG_C
Cuts at: 2422 2649 5006 5502 2422
Size: 227 2357 496 2723
Fragments arranged by size:
2723 2357 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2004 2590 1163
Size: 573 268 586 4376
Fragments arranged by size:
4376 586 573 268
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 3253 4014 5042 1540
Size: 275 186 108 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186 108
BsiHKAI G_wGCw'C
Cuts at: 3055 3864 3949 5020 3055
Size: 809 85 1071 3838
Fragments arranged by size:
3838 1071 809 85
BsmI GAATG_Cn'
Cuts at: 2467 3314 3407 2467
Size: 847 93 4863
Fragments arranged by size:
4863 847 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3058 3058
Size: 5803
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2396 2492 2934 2964 3055 3122
Size: 399 369 237 96 442 30 91 67
Cuts at: 3122 3864 3949 5020 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 369 237 96
91 85 67 30
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2490 2612 3158 844
Size: 1646 122 546 3489
Fragments arranged by size:
3489 1646 546 122
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2655 4447 4634 1700
Size: 110 349 496 1792 187 2869
Fragments arranged by size:
2869 1792 496 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 2058 2895 3133 3500 3885 3902 5354 5440
Size: 86 837 238 367 385 17 1452 86
Cuts at: 5440 5452 5798 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 837 385 367 346 238 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2120 2275 2906 513
Size: 552 939 116 155 631 3410
Fragments arranged by size:
3410 939 631 552 155 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2248 2821 2837 3319 4113 4133 2142
Size: 106 573 16 482 794 20 3812
Fragments arranged by size:
3812 794 573 482 106 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2657 3250
Size: 54 1425 50 275 18 276 551 593
Cuts at: 3250 4139 5768 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 593 551 276 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2106 3250 1537
Size: 275 294 1144 4090
Fragments arranged by size:
4090 1144 294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3733 1353
Size: 2380 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3053 3053
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2197 2819 2936 3866 4463 5250
Size: 393 431 134 622 117 930 597 787
Cuts at: 5250 5518 1239
Size: 268 1524
Fragments arranged by size:
1524 930 787 622 597 431 393 268
134 117
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2285
Size: 5803
EcoO109I rG'GnC_Cy
Cuts at: 2393 2479 2393
Size: 86 5717
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 2145 2656 4950 1678
Size: 467 511 2294 2531
Fragments arranged by size:
2531 2294 511 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2627 2659 2695
Size: 32 135 32 776 32 247 32 36
Cuts at: 2695 2727 2887 2919 4444 4476 4518 4550 1373
Size: 32 160 32 1525 32 42 32 2626
Fragments arranged by size:
2626 1525 776 247 160 135 42 36
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2594 748
Size: 1846 3957
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 2617 3128 2617
Size: 511 5292
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2433 2925 3761 4120 4279 518
Size: 648 1146 121 492 836 359 159 2042
Fragments arranged by size:
2042 1146 836 648 492 359 159 121
MspA1I CmG'CkG
Cuts at: 1606 2122 2755 3011 3219 3896 5044 5289 1606
Size: 516 633 256 208 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 516 256 245 208
NaeI GCC'GGC
Cuts at: 2657 2657
Size: 5803
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NgoMIV G'CCGG_C
Cuts at: 2655 2655
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2272 2313 3161 4706 1132
Size: 97 1043 41 848 1545 2229
Fragments arranged by size:
2229 1545 1043 848 97 41
PasI CC'CwG_GG
Cuts at: 1348 2396 1348
Size: 1048 4755
PciI A'CATG_T
Cuts at: 2309 4702 2309
Size: 2393 3410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2179 1108
Size: 1071 4732
PfoI T'CCnGG_A
Cuts at: 2376 2713 2376
Size: 337 5466
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2255 2287 3872 3904 5385 5417 2255
Size: 32 1585 32 1481 32 2641
Fragments arranged by size:
2641 1585 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2479
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2392 2392
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2487 3155 830
Size: 1657 668 3478
Fragments arranged by size:
3478 1657 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2755 3011 2755
Size: 256 5547
SacI G_AGCT'C
Cuts at: 3055 3055
Size: 5803
SacII CC_GC'GG
Cuts at: 2123 2123
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SbfI CC_TGCA'GG
Cuts at: 2487 3155 2487
Size: 668 5135
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SexAI A'CCwGG_T
Cuts at: 2619 2619
Size: 5803
SfcI C'TryA_G
Cuts at: 826 1045 2483 3151 4286 4967 5158 826
Size: 219 1438 668 1135 681 191 1471
Fragments arranged by size:
1471 1438 1135 681 668 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 2159 1700
Size: 459 5344
SmaI CCC'GGG
Cuts at: 1872 2256 3130 1872
Size: 384 874 4545
Fragments arranged by size:
4545 874 384
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2881 2943 3907 4808 5070 820
Size: 197 926 938 62 964 901 262 1553
Fragments arranged by size:
1553 964 938 926 901 262 197 62
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SrfI GCCC'GGGC
Cuts at: 2256 2256
Size: 5803
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2004 3000 3046 3242 513
Size: 552 939 996 46 196 3074
Fragments arranged by size:
3074 996 939 552 196 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3830 3847 4000 4185 4630 325
Size: 1255 2250 17 153 185 445 1498
Fragments arranged by size:
2250 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 4243
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 2254 3128 1870
Size: 384 874 4545
Fragments arranged by size:
4545 874 384
XmnI GAAnn'nnTTC
Cuts at: 2210 3932 2210
Size: 1722 4081
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SapI SfiI SfoI SwaI
XcmI