(Linear) (Six-base) MAPSORT of: KIEE2681.seq Check: 8528 from: 1 to: 1098 KIEE2681.orf With 182 enzymes: SgfI * March 10, 2017 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 607 1098 Size: 607 491 AccI GT'mk_AC Cuts at: 0 780 1098 Size: 780 318 Acc65I G'GTAC_C Cuts at: 0 608 1098 Size: 608 490 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 192 814 1098 Size: 58 134 622 284 Fragments arranged by size: 622 284 134 58 AflIII A'CryG_T Cuts at: 0 304 1098 Size: 304 794 AleI CACnn'nnGTG Cuts at: 0 307 1098 Size: 307 791 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 250 282 1098 Size: 250 32 816 Fragments arranged by size: 816 250 32 AlwNI CAG_nnn'CTG Cuts at: 0 174 332 1098 Size: 174 158 766 Fragments arranged by size: 766 174 158 ApaI G_GGCC'C Cuts at: 0 391 1098 Size: 391 707 AvaI C'yCGr_G Cuts at: 0 249 1023 1098 Size: 249 774 75 Fragments arranged by size: 774 249 75 BamHI G'GATC_C Cuts at: 0 890 1098 Size: 890 208 BanI G'GyrC_C Cuts at: 0 40 151 292 608 954 1098 Size: 40 111 141 316 346 144 Fragments arranged by size: 346 316 144 141 111 40 BanII G_rGCy'C Cuts at: 0 391 487 929 1050 1098 Size: 391 96 442 121 48 Fragments arranged by size: 442 391 121 96 48 BbsI GAAGACnn'nnnn_ Cuts at: 0 971 1098 Size: 971 127 BciVI GTATCCnnnnn_n' Cuts at: 0 875 1098 Size: 875 223 Bme1580I G_kGCm'C Cuts at: 0 154 391 959 1098 Size: 154 237 568 139 Fragments arranged by size: 568 237 154 139 BmrI ACTGGGnnnn_n' Cuts at: 0 59 793 1098 Size: 59 734 305 Fragments arranged by size: 734 305 59 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 1098 Size: 931 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 897 923 1098 Size: 897 26 175 Fragments arranged by size: 897 175 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 658 1098 Size: 157 501 440 Fragments arranged by size: 501 440 157 BsaHI Gr'CG_yC Cuts at: 0 777 1098 Size: 777 321 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 250 280 557 587 690 720 1098 Size: 250 30 277 30 103 30 378 Fragments arranged by size: 378 277 250 103 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 139 1098 Size: 139 959 BseYI C'CCAG_C Cuts at: 0 417 644 1098 Size: 417 227 454 Fragments arranged by size: 454 417 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 585 1098 Size: 585 513 BsiEI CG_ry'CG Cuts at: 0 104 1098 Size: 104 994 BsiHKAI G_wGCw'C Cuts at: 0 1050 1098 Size: 1050 48 BsmI GAATG_Cn' Cuts at: 0 462 1098 Size: 462 636 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1053 1098 Size: 1053 45 Bsp1286I G_dGCh'C Cuts at: 0 154 391 487 929 959 1050 1098 Size: 154 237 96 442 30 91 48 Fragments arranged by size: 442 237 154 96 91 48 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 485 607 1098 Size: 485 122 491 Fragments arranged by size: 491 485 122 BsrFI r'CCGG_y Cuts at: 0 154 650 1098 Size: 154 496 448 Fragments arranged by size: 496 448 154 BstYI r'GATC_y Cuts at: 0 53 890 1098 Size: 53 837 208 Fragments arranged by size: 837 208 53 BtgI C'CryG_G Cuts at: 0 115 270 901 1098 Size: 115 155 631 197 Fragments arranged by size: 631 197 155 115 BtsI GCAGTG_nn' Cuts at: 0 137 243 816 832 1098 Size: 137 106 573 16 266 Fragments arranged by size: 573 266 137 106 16 EaeI y'GGCC_r Cuts at: 0 101 652 1098 Size: 101 551 446 Fragments arranged by size: 551 446 101 EagI C'GGCC_G Cuts at: 0 101 1098 Size: 101 997 EcoICRI GAG'CTC Cuts at: 0 1048 1098 Size: 1048 50 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 192 814 931 1098 Size: 58 134 622 117 167 Fragments arranged by size: 622 167 134 117 58 EcoNI CCTnn'n_nnAGG Cuts at: 0 280 1098 Size: 280 818 EcoO109I rG'GnC_Cy Cuts at: 0 388 474 1098 Size: 388 86 624 Fragments arranged by size: 624 388 86 HaeII r_GCGC'y Cuts at: 0 140 651 1098 Size: 140 511 447 Fragments arranged by size: 511 447 140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 343 375 622 654 690 722 882 914 Size: 343 32 247 32 36 32 160 32 Cuts at: 914 1098 Size: 184 Fragments arranged by size: 343 247 184 160 36 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 589 1098 Size: 589 509 KpnI G_GTAC'C Cuts at: 0 612 1098 Size: 612 486 MslI CAynn'nnrTG Cuts at: 0 307 428 920 1098 Size: 307 121 492 178 Fragments arranged by size: 492 307 178 121 MspA1I CmG'CkG Cuts at: 0 117 750 1006 1098 Size: 117 633 256 92 Fragments arranged by size: 633 256 117 92 NaeI GCC'GGC Cuts at: 0 652 1098 Size: 652 446 NgoMIV G'CCGG_C Cuts at: 0 650 1098 Size: 650 448 NspI r_CATG'y Cuts at: 0 267 308 1098 Size: 267 41 790 Fragments arranged by size: 790 267 41 PasI CC'CwG_GG Cuts at: 0 391 1098 Size: 391 707 PciI A'CATG_T Cuts at: 0 304 1098 Size: 304 794 PflMI CCAn_nnn'nTGG Cuts at: 0 174 1098 Size: 174 924 PfoI T'CCnGG_A Cuts at: 0 371 708 1098 Size: 371 337 390 Fragments arranged by size: 390 371 337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 250 282 1098 Size: 250 32 816 Fragments arranged by size: 816 250 32 PpuMI rG'GwC_Cy Cuts at: 0 474 1098 Size: 474 624 PspOMI G'GGCC_C Cuts at: 0 387 1098 Size: 387 711 PstI C_TGCA'G Cuts at: 0 482 1098 Size: 482 616 PvuII CAG'CTG Cuts at: 0 750 1006 1098 Size: 750 256 92 Fragments arranged by size: 750 256 92 SacI G_AGCT'C Cuts at: 0 1050 1098 Size: 1050 48 SacII CC_GC'GG Cuts at: 0 118 1098 Size: 118 980 SbfI CC_TGCA'GG Cuts at: 0 482 1098 Size: 482 616 SexAI A'CCwGG_T Cuts at: 0 614 1098 Size: 614 484 SfcI C'TryA_G Cuts at: 0 478 1098 Size: 478 620 SgrAI Cr'CCGG_yG Cuts at: 0 154 1098 Size: 154 944 SmaI CCC'GGG Cuts at: 0 251 1098 Size: 251 847 SmlI C'TyrA_G Cuts at: 0 876 938 1098 Size: 876 62 160 Fragments arranged by size: 876 160 62 SrfI GCCC'GGGC Cuts at: 0 251 1098 Size: 251 847 StyI C'CwwG_G Cuts at: 0 995 1041 1098 Size: 995 46 57 Fragments arranged by size: 995 57 46 Tth111I GACn'n_nGTC Cuts at: 0 871 1098 Size: 871 227 XmaI C'CCGG_G Cuts at: 0 249 1098 Size: 249 849 XmnI GAAnn'nnTTC Cuts at: 0 205 1098 Size: 205 893 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaI AvaI BamHI BanI BanII BbsI BciVI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KpnI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SbfI SexAI SfcI SgrAI SmaI SmlI SrfI StyI Tth111I XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI XbaI XcmI XhoI ZraI