################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 781 GTCTAC GT^[AC][GT]AC AcyI 1063 GGCGCC G[GA]^CG[CT]C BamHI 383 GGATCC G^GATCC BanI 1063 GGCGCC G^G[CT][GA]CC BbeI 1063 GGCGCC GGCGC^C BbsI 579 GAAGAC GAAGAC(2/6) BbvCI 204 CCTCAGC CCTCAGC(-5/-2) BclI 92 TGATCA T^GATCA BglI 979 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 579 GAAGAC GAAGAC(2/6) BsaHI 1063 GGCGCC G[GA]^CG[CT]C BsgI 102 GTGCAG GTGCAG(16/14) BsmAI 1089 GAGAC GTCTC(1/5) BsmI 515 GAATGC GAATGC(1/-1) BspHI 1100 TCATGA T^CATGA BsrFI 857 ACCGGC [GA]^CCGG[CT] BssSI 678 CACGAG CACGAG(-5/-1) Cfr10I 857 ACCGGC [GA]^CCGG[CT] DraIII 96 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1155 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 92 TGATCA T^GATCA Hin1I 1063 GGCGCC G[GA]^CG[CT]C KasI 1063 GGCGCC G^GCGCC MaeII 68 ACGT A^CGT MboII 579 GAAGA GAAGA(8/7) MseI 1049 TTAA T^TAA MslI 510 CACCGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1063 GGCGCC GG^CGCC NcoI 26 CCATGG C^CATGG NspI 410 GCATGT [GA]CATG^[CT] PflMI 1149 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1100 TCATGA T^CATGA SfoI 1063 GGCGCC GGC^GCC SmaI 362 CCCGGG CCC^GGG Tru9I 1049 TTAA T^TAA Tsp509I 178 AATT ^AATT Van91I 1149 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 23 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 362 CCCGGG C^CCGGG XmaI 362 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 546 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1150 CAGCGCCTG AvaI 362 CCCGGG C^[CT]CG[GA]G 697 CCCGAG BsaWI 511 ACCGGA [AT]^CCGG[AT] 1085 ACCGGA BsoBI 362 CCCGGG C^[CT]CG[GA]G 697 CCCGAG BspDI 151 ATCGAT AT^CGAT 595 ATCGAT BspMI 104 GCAGGT ACCTGC(4/8) 235 GCAGGT BsrBI 715 CCGCTC CCGCTC(-3/-3) 655 GAGCGG BstF5I 504 CATCC GGATG(2/0) 492 GGATG BstXI 26 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 241 CCAGTGAGCTGG BstYI 200 AGATCC [GA]^GATC[CT] 383 GGATCC ClaI 151 ATCGAT AT^CGAT 595 ATCGAT DraII 839 AGGACCT [GA]G^G[ACGT]CC[CT] 1188 GGGTCCC EcoO109I 839 AGGACCT [GA]G^G[ACGT]CC[CT] 1188 GGGTCCC FokI 504 CATCC GGATG(9/13) 492 GGATG HgaI 55 GCGTC GACGC(5/10) 816 GCGTC MflI 200 AGATCC [GA]^GATC[CT] 383 GGATCC PpuMI 839 AGGACCT [GA]G^G[AT]CC[CT] 1188 GGGTCCC SacI 1003 GAGCTC GAGCT^C 1137 GAGCTC SfaNI 332 GCATC GCATC(5/9) 776 GCATC XhoII 200 AGATCC [GA]^GATC[CT] 383 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I