(Circular) (Six-base) MAPSORT of: pFN21AE3968.seq Check: 4665 from: 1 to: 5920 pFN21AE3968 5920 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3730 278 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 3263 1937 Size: 850 476 4594 Fragments arranged by size: 4594 850 476 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 4047 4420 1563 Size: 2484 373 3063 Fragments arranged by size: 3063 2484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2855 2861 3150 3983 5367 5635 1632 Size: 901 322 6 289 833 1384 268 1917 Fragments arranged by size: 1917 1384 901 833 322 289 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 4819 5796 1203 Size: 3616 977 1327 Fragments arranged by size: 3616 1327 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5920 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2330 1184 Size: 1146 4774 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 5888 AlwNI CAG_nnn'CTG Cuts at: 2556 3160 3663 5235 2556 Size: 604 503 1572 3241 Fragments arranged by size: 3241 1572 604 503 ApaLI G'TGCA_C Cuts at: 3977 5133 3977 Size: 1156 4764 ApoI r'AATT_y Cuts at: 1493 3229 3455 3607 5716 1493 Size: 1736 226 152 2109 1697 Fragments arranged by size: 2109 1736 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4474 160 Size: 872 3442 1606 Fragments arranged by size: 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 2367 2702 3245 1870 Size: 73 424 335 543 4545 Fragments arranged by size: 4545 543 424 335 73 BamHI G'GATC_C Cuts at: 2388 3250 3617 2388 Size: 862 367 4691 Fragments arranged by size: 4691 862 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3068 3241 4696 618 Size: 325 217 627 1281 173 1455 1842 Fragments arranged by size: 1842 1455 1281 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 2154 3012 3146 3189 3239 1391 Size: 763 858 134 43 50 4072 Fragments arranged by size: 4072 858 763 134 50 43 BbeI G_GCGC'C Cuts at: 3072 3072 Size: 5920 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2591 928 Size: 971 692 4257 Fragments arranged by size: 4257 971 692 BbvCI CC'TCA_GC Cuts at: 2210 2210 Size: 5920 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4111 4145 1903 Size: 34 2174 34 3678 Fragments arranged by size: 3678 2174 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 2097 1548 Size: 549 5371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2990 4531 136 Size: 107 122 71 631 605 1318 1541 1525 Fragments arranged by size: 1541 1525 1318 631 605 122 107 71 BglII A'GATC_T Cuts at: 5915 5915 Size: 5920 BlpI GC'TnA_GC Cuts at: 3337 3337 Size: 5920 Bme1580I G_kGCm'C Cuts at: 1790 2236 2319 3173 3981 5137 1790 Size: 446 83 854 808 1156 2573 Fragments arranged by size: 2573 1156 854 808 446 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5920 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2967 3053 3651 Size: 416 53 83 399 24 1550 86 598 Cuts at: 3651 4609 442 Size: 958 1753 Fragments arranged by size: 1753 1550 958 598 416 399 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5920 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2499 2828 2870 2903 2960 4580 1239 Size: 914 346 329 42 33 57 1620 2579 Fragments arranged by size: 2579 1620 914 346 329 57 42 33 Bpu10I CC'TnA_GC Cuts at: 2210 2210 Size: 5920 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4045 4910 5208 4045 Size: 865 298 4757 Fragments arranged by size: 4757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4583 882 Size: 617 3084 2219 Fragments arranged by size: 3084 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3711 493 Size: 711 2507 2702 Fragments arranged by size: 2702 2507 711 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3069 3727 4109 4744 Size: 53 83 186 936 1536 658 382 635 Cuts at: 4744 275 Size: 1451 Fragments arranged by size: 1536 1451 936 658 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2516 3090 4353 4751 5025 5172 5583 2516 Size: 574 1263 398 274 147 411 2853 Fragments arranged by size: 2853 1263 574 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 3107 3137 1178 Size: 30 165 30 1356 30 318 30 3961 Fragments arranged by size: 3961 1356 318 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2669 2672 2825 2900 3197 1186 Size: 1000 483 3 153 75 297 3909 Fragments arranged by size: 3909 1000 483 297 153 75 3 BseYI C'CCAG_C Cuts at: 2236 3153 5123 5619 2236 Size: 917 1970 496 2537 Fragments arranged by size: 2537 1970 917 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2128 1163 Size: 573 392 4955 Fragments arranged by size: 4955 573 392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3370 4131 5159 1540 Size: 275 186 1369 761 1028 2301 Fragments arranged by size: 2301 1369 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2709 3012 3113 3146 3981 4066 5137 2709 Size: 303 101 33 835 85 1071 3492 Fragments arranged by size: 3492 1071 835 303 101 85 33 BsmI GAATG_Cn' Cuts at: 2526 3431 3524 2526 Size: 905 93 4922 Fragments arranged by size: 4922 905 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2236 2319 2709 3012 3113 3146 Size: 399 364 82 83 390 303 101 33 Cuts at: 3146 3173 3189 3239 3981 4066 5137 1391 Size: 27 16 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 364 303 101 85 83 82 50 33 27 16 BspEI T'CCGG_A Cuts at: 5583 5583 Size: 5920 BspHI T'CATG_A Cuts at: 3105 3809 5539 3105 Size: 704 1730 3486 Fragments arranged by size: 3486 1730 704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 2231 3275 844 Size: 1256 131 1044 3489 Fragments arranged by size: 3489 1256 1044 131 BsrBI CCG'CTC Cuts at: 1394 1823 2662 2722 3807 1394 Size: 429 839 60 1085 3507 Fragments arranged by size: 3507 1085 839 429 60 BsrDI GCAATG_nn' Cuts at: 65 1248 4415 4589 65 Size: 1183 3167 174 1396 Fragments arranged by size: 3167 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2862 4564 4751 1700 Size: 110 1052 1702 187 2869 Fragments arranged by size: 2869 1702 1052 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 2683 3980 4992 2683 Size: 1297 1012 3611 Fragments arranged by size: 3611 1297 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5920 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5920 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2253 2038 Size: 215 5705 BstYI r'GATC_y Cuts at: 1972 2205 2388 3250 3617 4002 4019 5471 5557 Size: 233 183 862 367 385 17 1452 86 Cuts at: 5557 5569 5915 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 862 385 367 346 233 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2433 2592 513 Size: 552 939 27 402 159 3841 Fragments arranged by size: 3841 939 552 402 159 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5181 Fragments arranged by size: 5181 683 56 BtsI GCAGTG_nn' Cuts at: 3436 4230 4250 3436 Size: 794 20 5106 Fragments arranged by size: 5106 794 20 ClaI AT'CG_AT Cuts at: 1554 2157 2601 1554 Size: 603 444 4873 Fragments arranged by size: 4873 603 444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3224 3224 Size: 5920 DraIII CAC_nnn'GTG Cuts at: 2106 2106 Size: 5920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4927 809 Size: 507 3611 1802 Fragments arranged by size: 3611 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3367 4256 5885 Size: 54 1425 50 275 18 1537 889 1629 Cuts at: 5885 8 Size: 43 Fragments arranged by size: 1629 1537 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3367 1537 Size: 275 1555 4090 Fragments arranged by size: 4090 1555 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3850 1353 Size: 2497 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2870 4507 4881 5027 387 Size: 1064 138 1281 1637 374 146 1280 Fragments arranged by size: 1637 1281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3010 3144 3010 Size: 134 5786 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2499 2533 2828 2855 2861 2870 Size: 393 521 346 34 295 27 6 9 Cuts at: 2870 2903 2960 3150 3983 4580 5367 5635 1239 Size: 33 57 190 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 597 521 393 346 295 268 190 57 34 33 27 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3164 3164 Size: 5920 EcoO109I rG'GnC_Cy Cuts at: 2845 3194 2845 Size: 349 5571 EcoRI G'AATT_C Cuts at: 3229 3607 3229 Size: 378 5542 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 2541 2673 3072 3160 5067 1678 Size: 863 132 399 88 1907 2531 Fragments arranged by size: 2531 1907 863 399 132 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 3107 3139 4561 Size: 32 135 32 578 32 925 32 1422 Cuts at: 4561 4593 4635 4667 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 925 578 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3264 3510 669 Size: 988 281 1326 246 3079 Fragments arranged by size: 3079 1326 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KasI G'GCGC_C Cuts at: 3068 3068 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5034 5218 1305 Size: 3729 184 2007 Fragments arranged by size: 3729 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4098 Fragments arranged by size: 4098 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 3878 4237 4396 518 Size: 648 1353 1359 359 159 2042 Fragments arranged by size: 2042 1359 1353 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2275 2742 2839 2875 2881 2956 3121 Size: 541 128 467 97 36 6 75 165 Cuts at: 3121 3128 3336 4013 5161 5406 1606 Size: 7 208 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 541 467 245 208 165 128 97 75 36 7 6 NarI GG'CG_CC Cuts at: 3069 3069 Size: 5920 NcoI C'CATG_G Cuts at: 513 1065 2004 2031 513 Size: 552 939 27 4402 Fragments arranged by size: 4402 939 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5920 NotI GC'GGCC_GC Cuts at: 3367 3367 Size: 5920 NspI r_CATG'y Cuts at: 1132 1229 2419 3278 4823 1132 Size: 97 1190 859 1545 2229 Fragments arranged by size: 2229 1545 1190 859 97 PasI CC'CwG_GG Cuts at: 1348 2995 3189 1348 Size: 1647 194 4079 Fragments arranged by size: 4079 1647 194 PciI A'CATG_T Cuts at: 4819 4819 Size: 5920 PflMI CCAn_nnn'nTGG Cuts at: 1108 3160 1108 Size: 2052 3868 PfoI T'CCnGG_A Cuts at: 2574 2937 2574 Size: 363 5557 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 2791 3989 4021 5502 5534 2759 Size: 32 1198 32 1481 32 3145 Fragments arranged by size: 3145 1481 1198 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2845 3194 2845 Size: 349 5571 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5920 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PstI C_TGCA'G Cuts at: 830 2309 2880 2949 3272 830 Size: 1479 571 69 323 3478 Fragments arranged by size: 3478 1479 571 323 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 PvuII CAG'CTG Cuts at: 2742 2839 2875 2881 2956 3128 2742 Size: 97 36 6 75 172 5534 Fragments arranged by size: 5534 172 97 75 36 6 SacI G_AGCT'C Cuts at: 3012 3146 3012 Size: 134 5786 SalI G'TCGA_C Cuts at: 1936 3262 1936 Size: 1326 4594 SanDI GG'GwC_CC Cuts at: 3194 3194 Size: 5920 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SfcI C'TryA_G Cuts at: 826 1045 2305 2876 2945 3268 4403 5084 5275 Size: 219 1260 571 69 323 1135 681 191 Cuts at: 5275 826 Size: 1471 Fragments arranged by size: 1471 1260 1135 681 571 323 219 191 69 SfoI GGC'GCC Cuts at: 3070 3070 Size: 5920 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 2369 3247 1872 Size: 497 878 4545 Fragments arranged by size: 4545 878 497 SmlI C'TyrA_G Cuts at: 820 1017 1943 4024 4925 5187 820 Size: 197 926 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 3278 1132 Size: 2146 3774 SrfI GCCC'GGGC Cuts at: 2369 2369 Size: 5920 SspI AAT'ATT Cuts at: 5 52 3844 5 Size: 47 3792 2081 Fragments arranged by size: 3792 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5920 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2373 3003 3359 513 Size: 552 939 27 342 630 356 3074 Fragments arranged by size: 3074 939 630 552 356 342 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3947 3964 4117 4302 4747 325 Size: 1255 2367 17 153 185 445 1498 Fragments arranged by size: 2367 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 4166 5878 Size: 274 80 33 51 1443 162 2027 1712 Cuts at: 5878 96 Size: 138 Fragments arranged by size: 2027 1712 1443 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5880 5912 5880 Size: 32 5888 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5920 XbaI T'CTAG_A Cuts at: 3256 3256 Size: 5920 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2035 Size: 5920 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 2367 3245 1870 Size: 497 878 4545 Fragments arranged by size: 4545 878 497 XmnI GAAnn'nnTTC Cuts at: 4049 4049 Size: 5920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3728 276 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SwaI