(Linear) (Six-base) MAPSORT of: KIEE3968.seq Check: 6175 from: 1 to: 1215 KIEE3968.orf With 182 enzymes: SgfI * July 3, 2012 18:22 .. AccI GT'mk_AC Cuts at: 0 782 1215 Size: 782 433 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 528 850 856 1145 1215 Size: 528 322 6 289 70 Fragments arranged by size: 528 322 289 70 6 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 325 1215 Size: 325 890 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 754 786 1215 Size: 754 32 429 Fragments arranged by size: 754 429 32 AlwNI CAG_nnn'CTG Cuts at: 0 551 1155 1215 Size: 551 604 60 Fragments arranged by size: 604 551 60 AvaI C'yCGr_G Cuts at: 0 362 697 1215 Size: 362 335 518 Fragments arranged by size: 518 362 335 BamHI G'GATC_C Cuts at: 0 383 1215 Size: 383 832 BanI G'GyrC_C Cuts at: 0 1063 1215 Size: 1063 152 BanII G_rGCy'C Cuts at: 0 149 1007 1141 1184 1215 Size: 149 858 134 43 31 Fragments arranged by size: 858 149 134 43 31 BbeI G_GCGC'C Cuts at: 0 1067 1215 Size: 1067 148 BbsI GAAGACnn'nnnn_ Cuts at: 0 586 1215 Size: 586 629 BbvCI CC'TCA_GC Cuts at: 0 205 1215 Size: 205 1010 BclI T'GATC_A Cuts at: 0 92 1215 Size: 92 1123 BglI GCCn_nnn'nGGC Cuts at: 0 985 1215 Size: 985 230 Bme1580I G_kGCm'C Cuts at: 0 231 314 1168 1215 Size: 231 83 854 47 Fragments arranged by size: 854 231 83 47 BmrI ACTGGGnnnn_n' Cuts at: 0 962 1048 1215 Size: 962 86 167 Fragments arranged by size: 962 167 86 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 708 1215 Size: 708 507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 494 823 865 898 955 1215 Size: 148 346 329 42 33 57 260 Fragments arranged by size: 346 329 260 148 57 42 33 Bpu10I CC'TnA_GC Cuts at: 0 205 1215 Size: 205 1010 BsaHI Gr'CG_yC Cuts at: 0 1064 1215 Size: 1064 151 BsaWI w'CCGG_w Cuts at: 0 511 1085 1215 Size: 511 574 130 Fragments arranged by size: 574 511 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 754 784 1102 1132 1215 Size: 754 30 318 30 83 Fragments arranged by size: 754 318 83 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 181 664 667 820 895 1192 1215 Size: 181 483 3 153 75 297 23 Fragments arranged by size: 483 297 181 153 75 23 3 BseYI C'CCAG_C Cuts at: 0 231 1148 1215 Size: 231 917 67 Fragments arranged by size: 917 231 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 123 1215 Size: 123 1092 BsiHKAI G_wGCw'C Cuts at: 0 704 1007 1108 1141 1215 Size: 704 303 101 33 74 Fragments arranged by size: 704 303 101 74 33 BsmI GAATG_Cn' Cuts at: 0 521 1215 Size: 521 694 Bsp1286I G_dGCh'C Cuts at: 0 149 231 314 704 1007 1108 1141 1168 Size: 149 82 83 390 303 101 33 27 Cuts at: 1168 1184 1215 Size: 16 31 Fragments arranged by size: 390 303 149 101 83 82 33 31 27 16 BspHI T'CATG_A Cuts at: 0 1100 1215 Size: 1100 115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 95 226 1215 Size: 95 131 989 Fragments arranged by size: 989 131 95 BsrBI CCG'CTC Cuts at: 0 657 717 1215 Size: 657 60 498 Fragments arranged by size: 657 498 60 BsrFI r'CCGG_y Cuts at: 0 857 1215 Size: 857 358 BssSI C'ACGA_G Cuts at: 0 678 1215 Size: 678 537 BstXI CCAn_nnnn'nTGG Cuts at: 0 33 248 1215 Size: 33 215 967 Fragments arranged by size: 967 215 33 BstYI r'GATC_y Cuts at: 0 200 383 1215 Size: 200 183 832 Fragments arranged by size: 832 200 183 BtgI C'CryG_G Cuts at: 0 26 428 587 1215 Size: 26 402 159 628 Fragments arranged by size: 628 402 159 26 ClaI AT'CG_AT Cuts at: 0 152 596 1215 Size: 152 444 619 Fragments arranged by size: 619 444 152 DraIII CAC_nnn'GTG Cuts at: 0 101 1215 Size: 101 1114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 865 1215 Size: 865 350 EcoICRI GAG'CTC Cuts at: 0 1005 1139 1215 Size: 1005 134 76 Fragments arranged by size: 1005 134 76 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 494 528 823 850 856 865 898 Size: 148 346 34 295 27 6 9 33 Cuts at: 898 955 1145 1215 Size: 57 190 70 Fragments arranged by size: 346 295 190 148 70 57 34 33 27 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1159 1215 Size: 1159 56 EcoO109I rG'GnC_Cy Cuts at: 0 840 1189 1215 Size: 840 349 26 Fragments arranged by size: 840 349 26 HaeII r_GCGC'y Cuts at: 0 536 668 1067 1155 1215 Size: 536 132 399 88 60 Fragments arranged by size: 536 399 132 88 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 145 177 1102 1134 1215 Size: 145 32 925 32 81 Fragments arranged by size: 925 145 81 32 32 KasI G'GCGC_C Cuts at: 0 1063 1215 Size: 1063 152 MslI CAynn'nnrTG Cuts at: 0 514 1215 Size: 514 701 MspA1I CmG'CkG Cuts at: 0 142 270 737 834 870 876 951 1116 Size: 142 128 467 97 36 6 75 165 Cuts at: 1116 1123 1215 Size: 7 92 Fragments arranged by size: 467 165 142 128 97 92 75 36 7 6 NarI GG'CG_CC Cuts at: 0 1064 1215 Size: 1064 151 NcoI C'CATG_G Cuts at: 0 26 1215 Size: 26 1189 NspI r_CATG'y Cuts at: 0 414 1215 Size: 414 801 PasI CC'CwG_GG Cuts at: 0 990 1184 1215 Size: 990 194 31 Fragments arranged by size: 990 194 31 PflMI CCAn_nnn'nTGG Cuts at: 0 1155 1215 Size: 1155 60 PfoI T'CCnGG_A Cuts at: 0 569 932 1215 Size: 569 363 283 Fragments arranged by size: 569 363 283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 754 786 1215 Size: 754 32 429 Fragments arranged by size: 754 429 32 PpuMI rG'GwC_Cy Cuts at: 0 840 1189 1215 Size: 840 349 26 Fragments arranged by size: 840 349 26 PstI C_TGCA'G Cuts at: 0 304 875 944 1215 Size: 304 571 69 271 Fragments arranged by size: 571 304 271 69 PvuII CAG'CTG Cuts at: 0 737 834 870 876 951 1123 1215 Size: 737 97 36 6 75 172 92 Fragments arranged by size: 737 172 97 92 75 36 6 SacI G_AGCT'C Cuts at: 0 1007 1141 1215 Size: 1007 134 74 Fragments arranged by size: 1007 134 74 SanDI GG'GwC_CC Cuts at: 0 1189 1215 Size: 1189 26 SfcI C'TryA_G Cuts at: 0 300 871 940 1215 Size: 300 571 69 275 Fragments arranged by size: 571 300 275 69 SfoI GGC'GCC Cuts at: 0 1065 1215 Size: 1065 150 SmaI CCC'GGG Cuts at: 0 364 1215 Size: 364 851 SrfI GCCC'GGGC Cuts at: 0 364 1215 Size: 364 851 StyI C'CwwG_G Cuts at: 0 26 368 998 1215 Size: 26 342 630 217 Fragments arranged by size: 630 342 217 26 TatI w'GTAC_w Cuts at: 0 134 1215 Size: 134 1081 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 30 1215 Size: 30 1185 XmaI C'CCGG_G Cuts at: 0 362 1215 Size: 362 853 Enzymes that do cut: AccI AcuI AlfI AloI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI ClaI DraIII EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PpiI PpuMI PstI PvuII SacI SanDI SfcI SfoI SmaI SrfI StyI TatI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI