################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BcgI BciVI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FspI HindIII HpaI KspI MaeI MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 586 GGTACC G^GTACC AccI 328 GTCTAC GT^[AC][GT]AC AcsI 715 AAATTC [GA]^AATT[CT] AcyI 130 GGCGCC G[GA]^CG[CT]C AlwNI 1216 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 715 AAATTC [GA]^AATT[CT] Asp718I 586 GGTACC G^GTACC AvaI 1050 CCCGGG C^[CT]CG[GA]G BamHI 1172 GGATCC G^GATCC BbeI 130 GGCGCC GGCGC^C BbvCI 810 CCTCAGC CCTCAGC(-5/-2) BclI 47 TGATCA T^GATCA BglI 671 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 147 GCTGAGC GC^T[ACGT]AGC Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC BsaHI 130 GGCGCC G[GA]^CG[CT]C BsiEI 753 CGACCG CG[GA][CT]^CG BsmFI 311 GGGAC GGGAC(10/14) BsoBI 1050 CCCGGG C^[CT]CG[GA]G BspMI 539 ACCTGC ACCTGC(4/8) BsrFI 1121 GCCGGC [GA]^CCGG[CT] BstAPI 93 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1210 GGTTACC G^GT[ACGT]ACC BstPI 1210 GGTTACC G^GT[ACGT]ACC CelII 147 GCTGAGC GC^T[ACGT]AGC Cfr10I 1121 GCCGGC [GA]^CCGG[CT] DraII 1217 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 153 CCACGG C^C[GA][CT]GG EcoO109I 1217 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1210 GGTTACC G^GT[ACGT]ACC EcoRV 939 GATATC GAT^ATC EcoT14I 896 CCAAGG C^C[AT][AT]GG FbaI 47 TGATCA T^GATCA FseI 1120 GGCCGGCC GGCCGG^CC HgaI 563 GCGTC GACGC(5/10) Hin1I 130 GGCGCC G[GA]^CG[CT]C HincII 535 GTCAAC GT[CT]^[GA]AC HindII 535 GTCAAC GT[CT]^[GA]AC KasI 130 GGCGCC G^GCGCC KpnI 586 GGTACC GGTAC^C NaeI 1121 GCCGGC GCC^GGC NarI 130 GGCGCC GG^CGCC NgoMIV 1121 GCCGGC G^CCGGC PstI 391 CTGCAG CTGCA^G SapI 654 GCTCTTC GCTCTTC(1/4) SbfI 390 CCTGCAGG CCTGCA^GG ScaI 1064 AGTACT AGT^ACT SfcI 391 CTGCAG C^T[GA][CT]AG SfoI 130 GGCGCC GGC^GCC SmaI 1050 CCCGGG CCC^GGG SmlI 598 CTCAAG C^T[CT][GA]AG Sse8387I 390 CCTGCAGG CCTGCA^GG StyI 896 CCAAGG C^C[AT][AT]GG TfiI 977 GATTC G^A[AT]TC Tsp509I 716 AATT ^AATT XcmI 224 CCAATGCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1050 CCCGGG C^CCGGG XmaI 1050 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 649 CGCG CG^CG 874 CGCG AvaII 162 GGTCC G^G[AT]CC 1179 GGTCC BbsI 16 GTCTTC GAAGAC(2/6) 511 GTCTTC BpuAI 16 GTCTTC GAAGAC(2/6) 511 GTCTTC BsmAI 427 GAGAC GTCTC(1/5) 1165 GTCTC BsmBI 1164 CGTCTC CGTCTC(1/5) 427 GAGACG BsmI 219 GAATGC GAATGC(1/-1) 617 GAATGC BstUI 649 CGCG CG^CG 874 CGCG BstXI 829 CCACTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 926 CCAACGGTGTGG DrdI 313 GACAACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 429 GACGGGCAAGTC EarI 655 CTCTTC CTCTTC(1/4) 1108 GAAGAG Ksp632I 655 CTCTTC CTCTTC(1/4) 1108 GAAGAG MaeII 29 ACGT A^CGT 1022 ACGT MspA1I 115 CAGCGG C[AC]G^C[GT]G 142 CCGCTG MvnI 649 CGCG CG^CG 874 CGCG TaqI 214 TCGA T^CGA 659 TCGA TthHB8I 214 TCGA T^CGA 659 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI XhoII