Restriction map for
pFN21AE4412
(Circular) (Six-base) MAPSORT of: pFN21AE4412.seq Check: 1331 from: 1 to: 5980
pFN21AE4412 5980 bp
With 182 enzymes: SgfI *
March 28, 2012 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 3323 1937
Size: 397 989 4594
Fragments arranged by size:
4594 989 397
Acc65I G'GTAC_C
Cuts at: 2591 3301 2591
Size: 710 5270
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4043 5427 5695 1632
Size: 2411 1384 268 1917
Fragments arranged by size:
2411 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4753
AlwNI CAG_nnn'CTG
Cuts at: 3226 3723 5295 3226
Size: 497 1572 3911
Fragments arranged by size:
3911 1572 497
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2720 3289 3515 3667 5776 1493
Size: 1227 569 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 569 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3055 3305 1870
Size: 73 1112 250 4545
Fragments arranged by size:
4545 1112 250 73
BamHI G'GATC_C
Cuts at: 3177 3310 3677 3177
Size: 133 367 5480
Fragments arranged by size:
5480 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 2449 2591 3301 4756
Size: 325 217 627 348 314 142 710 1455
Cuts at: 4756 618
Size: 1842
Fragments arranged by size:
1842 1455 710 627 348 325 314 217
142
BanII G_rGCy'C
Cuts at: 1391 2355 2670 2802 3085 3299 1391
Size: 964 315 132 283 214 4072
Fragments arranged by size:
4072 964 315 283 214 132
BbeI G_GCGC'C
Cuts at: 2139 2139
Size: 5980
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 2509 928
Size: 971 115 495 4399
Fragments arranged by size:
4399 971 495 115
BbvCI CC'TCA_GC
Cuts at: 2816 2816
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 2052 1548
Size: 504 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2682 4591 136
Size: 107 122 71 631 605 1010 1909 1525
Fragments arranged by size:
1909 1525 1010 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 2153 3397 2153
Size: 1244 4736
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2450 2729 4640 1239
Size: 1079 132 279 1911 2579
Fragments arranged by size:
2579 1911 1079 279 132
Bpu10I CC'TnA_GC
Cuts at: 2816 2816
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 4105 4970 5268 2588
Size: 1517 865 298 3300
Fragments arranged by size:
3300 1517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 3787 4169 4804
Size: 53 83 186 936 603 1651 382 635
Cuts at: 4804 275
Size: 1451
Fragments arranged by size:
1651 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2315 2318 1186
Size: 955 174 3 4848
Fragments arranged by size:
4848 955 174 3
BseYI C'CCAG_C
Cuts at: 2638 2960 5183 5679 2638
Size: 322 2223 496 2939
Fragments arranged by size:
2939 2223 496 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3430 4191 5219 1540
Size: 275 186 760 669 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 669 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2355 2661 2670 2802 4041 4126 5197 2100
Size: 255 306 9 132 1239 85 1071 2883
Fragments arranged by size:
2883 1239 1071 306 255 132 85 9
BsmI GAATG_Cn'
Cuts at: 2230 2628 3491 3584 2230
Size: 398 863 93 4626
Fragments arranged by size:
4626 863 398 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2426 3175 2426
Size: 749 5231
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2355 2661 2670 2802 3085 3299
Size: 399 310 255 306 9 132 283 214
Cuts at: 3299 4041 4126 5197 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 306 283 255
214 132 85 9
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 3335 844
Size: 1709 782 3489
Fragments arranged by size:
3489 1709 782
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3126 4624 4811 1700
Size: 110 1316 1498 187 2869
Fragments arranged by size:
2869 1498 1316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 794
Size: 1310 4670
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 3215 1184
Size: 2031 3949
BstXI CCAn_nnnn'nTGG
Cuts at: 2841 2938 2841
Size: 97 5883
BstYI r'GATC_y
Cuts at: 1972 2391 3153 3177 3310 3677 4062 4079 5531
Size: 419 762 24 133 367 385 17 1452
Cuts at: 5531 5617 5629 5975 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 762 419 385 367 346 133
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2158 513
Size: 552 1093 4335
Fragments arranged by size:
4335 1093 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2088 2979 487
Size: 683 56 862 891 3488
Fragments arranged by size:
3488 891 862 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4290 4310 3496
Size: 794 20 5166
Fragments arranged by size:
5166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 2440 4987 809
Size: 507 1008 116 2547 1802
Fragments arranged by size:
2547 1802 1008 507 116
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2161 2208
Size: 54 1425 50 275 18 249 82 47
Cuts at: 2208 2928 3427 4316 5945 8
Size: 720 499 889 1629 43
Fragments arranged by size:
1629 1425 889 720 499 275 249 82
54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3108 3910 1353
Size: 1313 442 802 3423
Fragments arranged by size:
3423 1313 802 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 1999 4567 4941 5087 387
Size: 1064 138 410 2568 374 146 1280
Fragments arranged by size:
2568 1280 1064 410 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2668 2800 2353
Size: 315 132 5533
Fragments arranged by size:
5533 315 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2450 2729 4043 4640 5427 5695
Size: 393 686 132 279 1314 597 787 268
Cuts at: 5695 1239
Size: 1524
Fragments arranged by size:
1524 1314 787 686 597 393 279 268
132
EcoO109I rG'GnC_Cy
Cuts at: 3223 3223
Size: 5980
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
EcoRV GAT'ATC
Cuts at: 2946 2946
Size: 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5980
FseI GG_CCGG'CC
Cuts at: 3130 3130
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2139 2485 3006 5127 1678
Size: 461 346 521 2121 2531
Fragments arranged by size:
2531 2121 521 461 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 2957 2989 4621
Size: 32 135 32 1217 32 136 32 1632
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1632 1217 136 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 3324 3570 669
Size: 988 281 604 782 246 3079
Fragments arranged by size:
3079 988 782 604 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KasI G'GCGC_C
Cuts at: 2135 2135
Size: 5980
KpnI G_GTAC'C
Cuts at: 2595 3305 2595
Size: 710 5270
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2210 2930 10
Size: 54 1425 343 249 129 720 3060
Fragments arranged by size:
3060 1425 720 343 249 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4297 4456 518
Size: 648 2772 359 159 2042
Fragments arranged by size:
2772 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2149 3396 4073 5221 5466 1606
Size: 516 27 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 516 245 27
NaeI GCC'GGC
Cuts at: 3128 3128
Size: 5980
NarI GG'CG_CC
Cuts at: 2136 2136
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5428
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 3126 3126
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3231 1348
Size: 1883 4097
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2313 2313
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2400 3332 830
Size: 1570 932 3478
Fragments arranged by size:
3478 1570 932
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SacI G_AGCT'C
Cuts at: 2355 2670 2802 2355
Size: 315 132 5533
Fragments arranged by size:
5533 315 132
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2666 2666
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 2400 3332 2400
Size: 932 5048
ScaI AGT'ACT
Cuts at: 3071 4228 5940 3071
Size: 1157 1712 3111
Fragments arranged by size:
3111 1712 1157
SfcI C'TryA_G
Cuts at: 826 1045 2396 3328 4463 5144 5335 826
Size: 219 1351 932 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 932 681 219 191
SfoI GGC'GCC
Cuts at: 2137 2137
Size: 5980
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3057 3307 1872
Size: 1185 250 4545
Fragments arranged by size:
4545 1185 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 4084 4985 5247 820
Size: 197 926 660 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 660 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2901 3419 513
Size: 552 1836 518 3074
Fragments arranged by size:
3074 1836 552 518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 2837 3263 4007 4024 4177 4362
Size: 1255 890 367 426 744 17 153 185
Cuts at: 4362 4807 325
Size: 445 1498
Fragments arranged by size:
1498 1255 890 744 445 426 367 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2403 3069
Size: 274 80 33 51 1443 267 159 666
Cuts at: 3069 4226 5938 96
Size: 1157 1712 138
Fragments arranged by size:
1712 1443 1157 666 274 267 159 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2236
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3055 3305 1870
Size: 1185 250 4545
Fragments arranged by size:
4545 1185 250
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI FspAI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SrfI SwaI