(Linear) (Six-base) MAPSORT of: KIEE4412.seq Check: 9988 from: 1 to: 1275 KIEE4412.orf With 182 enzymes: SgfI * March 22, 2012 17:56 .. AccI GT'mk_AC Cuts at: 0 329 1275 Size: 329 946 Acc65I G'GTAC_C Cuts at: 0 586 1275 Size: 586 689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 406 1275 Size: 406 869 AlwNI CAG_nnn'CTG Cuts at: 0 1221 1275 Size: 1221 54 ApoI r'AATT_y Cuts at: 0 715 1275 Size: 715 560 AvaI C'yCGr_G Cuts at: 0 1050 1275 Size: 1050 225 BamHI G'GATC_C Cuts at: 0 1172 1275 Size: 1172 103 BanI G'GyrC_C Cuts at: 0 130 444 586 1275 Size: 130 314 142 689 Fragments arranged by size: 689 314 142 130 BanII G_rGCy'C Cuts at: 0 350 665 797 1080 1275 Size: 350 315 132 283 195 Fragments arranged by size: 350 315 283 195 132 BbeI G_GCGC'C Cuts at: 0 134 1275 Size: 134 1141 BbsI GAAGACnn'nnnn_ Cuts at: 0 9 504 1275 Size: 9 495 771 Fragments arranged by size: 771 495 9 BbvCI CC'TCA_GC Cuts at: 0 811 1275 Size: 811 464 BclI T'GATC_A Cuts at: 0 47 1275 Size: 47 1228 BglI GCCn_nnn'nGGC Cuts at: 0 677 1275 Size: 677 598 BlpI GC'TnA_GC Cuts at: 0 148 1275 Size: 148 1127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 879 1275 Size: 879 396 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 445 724 1275 Size: 313 132 279 551 Fragments arranged by size: 551 313 279 132 Bpu10I CC'TnA_GC Cuts at: 0 811 1275 Size: 811 464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 1275 Size: 583 692 BsaHI Gr'CG_yC Cuts at: 0 131 1275 Size: 131 1144 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 310 313 1275 Size: 136 174 3 962 Fragments arranged by size: 962 174 136 3 BseYI C'CCAG_C Cuts at: 0 633 955 1275 Size: 633 322 320 Fragments arranged by size: 633 322 320 BsiEI CG_ry'CG Cuts at: 0 756 1275 Size: 756 519 BsiHKAI G_wGCw'C Cuts at: 0 95 350 656 665 797 1275 Size: 95 255 306 9 132 478 Fragments arranged by size: 478 306 255 132 95 9 BsmI GAATG_Cn' Cuts at: 0 225 623 1275 Size: 225 398 652 Fragments arranged by size: 652 398 225 BsmBI CGTCTCn'nnnn_ Cuts at: 0 421 1170 1275 Size: 421 749 105 Fragments arranged by size: 749 421 105 Bsp1286I G_dGCh'C Cuts at: 0 95 350 656 665 797 1080 1275 Size: 95 255 306 9 132 283 195 Fragments arranged by size: 306 283 255 195 132 95 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 548 1275 Size: 548 727 BsrFI r'CCGG_y Cuts at: 0 1121 1275 Size: 1121 154 BstAPI GCAn_nnn'nTGC Cuts at: 0 99 1275 Size: 99 1176 BstEII G'GTnAC_C Cuts at: 0 1210 1275 Size: 1210 65 BstXI CCAn_nnnn'nTGG Cuts at: 0 836 933 1275 Size: 836 97 342 Fragments arranged by size: 836 342 97 BstYI r'GATC_y Cuts at: 0 386 1148 1172 1275 Size: 386 762 24 103 Fragments arranged by size: 762 386 103 24 BtgI C'CryG_G Cuts at: 0 153 1275 Size: 153 1122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 83 974 1275 Size: 83 891 301 Fragments arranged by size: 891 301 83 DrdI GACnn_nn'nnGTC Cuts at: 0 319 435 1275 Size: 319 116 840 Fragments arranged by size: 840 319 116 EaeI y'GGCC_r Cuts at: 0 74 156 203 923 1275 Size: 74 82 47 720 352 Fragments arranged by size: 720 352 82 74 47 EarI CTCTTCn'nnn_ Cuts at: 0 661 1103 1275 Size: 661 442 172 Fragments arranged by size: 661 442 172 EcoICRI GAG'CTC Cuts at: 0 348 663 795 1275 Size: 348 315 132 480 Fragments arranged by size: 480 348 315 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 445 724 1275 Size: 313 132 279 551 Fragments arranged by size: 551 313 279 132 EcoO109I rG'GnC_Cy Cuts at: 0 1218 1275 Size: 1218 57 EcoRV GAT'ATC Cuts at: 0 941 1275 Size: 941 334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1083 1275 Size: 1083 192 FseI GG_CCGG'CC Cuts at: 0 1125 1275 Size: 1125 150 HaeII r_GCGC'y Cuts at: 0 134 480 1001 1275 Size: 134 346 521 274 Fragments arranged by size: 521 346 274 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 784 816 952 984 1275 Size: 784 32 136 32 291 Fragments arranged by size: 784 291 136 32 32 HincII GTy'rAC Cuts at: 0 537 1275 Size: 537 738 KasI G'GCGC_C Cuts at: 0 130 1275 Size: 130 1145 KpnI G_GTAC'C Cuts at: 0 590 1275 Size: 590 685 MscI TGG'CCA Cuts at: 0 76 205 925 1275 Size: 76 129 720 350 Fragments arranged by size: 720 350 129 76 MspA1I CmG'CkG Cuts at: 0 117 144 1275 Size: 117 27 1131 Fragments arranged by size: 1131 117 27 NaeI GCC'GGC Cuts at: 0 1123 1275 Size: 1123 152 NarI GG'CG_CC Cuts at: 0 131 1275 Size: 131 1144 NgoMIV G'CCGG_C Cuts at: 0 1121 1275 Size: 1121 154 PasI CC'CwG_GG Cuts at: 0 1226 1275 Size: 1226 49 PfoI T'CCnGG_A Cuts at: 0 308 1275 Size: 308 967 PstI C_TGCA'G Cuts at: 0 395 1275 Size: 395 880 SacI G_AGCT'C Cuts at: 0 350 665 797 1275 Size: 350 315 132 478 Fragments arranged by size: 478 350 315 132 SapI GCTCTTCn'nnn_ Cuts at: 0 661 1275 Size: 661 614 SbfI CC_TGCA'GG Cuts at: 0 395 1275 Size: 395 880 ScaI AGT'ACT Cuts at: 0 1066 1275 Size: 1066 209 SfcI C'TryA_G Cuts at: 0 391 1275 Size: 391 884 SfoI GGC'GCC Cuts at: 0 132 1275 Size: 132 1143 SmaI CCC'GGG Cuts at: 0 1052 1275 Size: 1052 223 SmlI C'TyrA_G Cuts at: 0 598 1275 Size: 598 677 StyI C'CwwG_G Cuts at: 0 896 1275 Size: 896 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 465 832 1258 1275 Size: 465 367 426 17 Fragments arranged by size: 465 426 367 17 TatI w'GTAC_w Cuts at: 0 239 398 1064 1275 Size: 239 159 666 211 Fragments arranged by size: 666 239 211 159 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 231 1275 Size: 231 1044 XmaI C'CCGG_G Cuts at: 0 1050 1275 Size: 1050 225 Enzymes that do cut: AccI Acc65I AlfI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BlpI BplI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FseI HaeII Hin4I HincII KasI KpnI MscI MspA1I NaeI NarI NgoMIV PasI PfoI PstI SacI SapI SbfI ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI FspI FspAI HindIII HpaI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI