################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BclI BfrI BglI BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 161 GACGTC GACGT^C AlwNI 463 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 570 AGCGCT AGC^GCT ApaI 179 GGGCCC GGGCC^C Asp700I 710 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 179 GGGCCC G[GA]GC[CT]^C BbvCI 289 GCTGAGG CCTCAGC(-5/-2) BglII 407 AGATCT A^GATCT BlpI 456 GCTGAGC GC^T[ACGT]AGC Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC BseRI 855 GAGGAG GAGGAG(10/8) BstAPI 266 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 314 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 456 GCTGAGC GC^T[ACGT]AGC EarI 510 CTCTTC CTCTTC(1/4) Eco47III 570 AGCGCT AGC^GCT FauI 262 GCGGG CCCGC(4/6) HphI 675 GGTGA GGTGA(8/7) Ksp632I 510 CTCTTC CTCTTC(1/4) MaeIII 121 GTCAC ^GT[ACGT]AC NcoI 227 CCATGG C^CATGG SalI 340 GTCGAC G^TCGAC SfaNI 795 GATGC GCATC(5/9) TaqI 341 TCGA T^CGA TfiI 769 GAATC G^A[AT]TC Tsp45I 121 GTCAC ^GT[GC]AC Tsp509I 648 AATT ^AATT TthHB8I 341 TCGA T^CGA XmnI 710 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 340 GTCGAC GT^[AC][GT]AC 550 GTCTAC AlwI 420 GATCC GGATC(4/5) 140 GGATC AvaI 17 CCCGGG C^[CT]CG[GA]G 170 CCCGGG BalI 230 TGGCCA TGG^CCA 311 TGGCCA BbrPI 184 CACGTG CAC^GTG 277 CACGTG BfaI 50 CTAG C^TAG 587 CTAG BpmI 790 CTCCAG CTGGAG(16/14) 322 CTGGAG BsaAI 184 CACGTG [CT]AC^GT[GA] 277 CACGTG BsaI 507 GGTCTC GGTCTC(1/5) 580 GGTCTC BsgI 354 GTGCAG GTGCAG(16/14) 763 GTGCAG BsoBI 17 CCCGGG C^[CT]CG[GA]G 170 CCCGGG BsrDI 516 CATTGC GCAATG(2/0) 821 CATTGC BstYI 407 AGATCT [GA]^GATC[CT] 419 AGATCC DraII 178 GGGGCCC [GA]G^G[ACGT]CC[CT] 317 AGGCCCT DsaI 116 CCGTGG C^C[GA][CT]GG 227 CCATGG EaeI 230 TGGCCA [CT]^GGCC[GA] 311 TGGCCA EcoNI 44 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 373 CCTCTGGTAGG EcoO109I 178 GGGGCCC [GA]G^G[ACGT]CC[CT] 317 AGGCCCT HaeII 540 GGCGCT [GA]GCGC^[CT] 570 AGCGCT HgaI 95 GCGTC GACGC(5/10) 338 GCGTC MaeI 50 CTAG C^TAG 587 CTAG MboII 710 GAAGA GAAGA(8/7) 511 TCTTC MflI 407 AGATCT [GA]^GATC[CT] 419 AGATCC MluNI 230 TGGCCA TGG^CCA 311 TGGCCA MscI 230 TGGCCA TGG^CCA 311 TGGCCA MspA1I 304 CAGCTG C[AC]G^C[GT]G 732 CAGCTG MwoI 23 GCTACCAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 266 GCATTGTGTGC PleI 699 GAGTC GAGTC(4/5) 750 GAGTC PmaCI 184 CACGTG CAC^GTG 277 CACGTG PmlI 184 CACGTG CAC^GTG 277 CACGTG PstI 238 CTGCAG CTGCA^G 735 CTGCAG PvuII 304 CAGCTG CAG^CTG 732 CAGCTG SfcI 238 CTGCAG C^T[GA][CT]AG 735 CTGCAG SmaI 17 CCCGGG CCC^GGG 170 CCCGGG XhoII 407 AGATCT [GA]^GATC[CT] 419 AGATCC XmaCI 17 CCCGGG C^CCGGG 170 CCCGGG XmaI 17 CCCGGG C^CCGGG 170 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcyI AluI AspHI AvaII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII ItaI MaeII MboI MnlI MslI MspI MvaI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI TspRI