(Circular) (Six-base) MAPSORT of: pFN21AE2722.seq Check: 1442 from: 1 to: 5605 pFN21AE2722 5605 bp With 182 enzymes: SgfI * June 6, 2018 18:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2170 3415 278 Size: 53 83 186 936 634 1245 2468 Fragments arranged by size: 2468 1245 936 634 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 2556 2948 1937 Size: 409 210 392 4594 Fragments arranged by size: 4594 409 392 210 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2653 3668 5052 5320 1632 Size: 1021 1015 1384 268 1917 Fragments arranged by size: 1917 1384 1021 1015 268 AfeI AGC'GCT Cuts at: 2577 2577 Size: 5605 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2429 2461 2429 Size: 32 5573 AlwNI CAG_nnn'CTG Cuts at: 2473 3348 4920 2473 Size: 875 1572 3158 Fragments arranged by size: 3158 1572 875 ApaI G_GGCC'C Cuts at: 2188 2188 Size: 5605 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2175 2930 1870 Size: 73 79 153 755 4545 Fragments arranged by size: 4545 755 153 79 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2188 2924 1391 Size: 797 736 4072 Fragments arranged by size: 4072 797 736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4634 BbvCI CC'TCA_GC Cuts at: 2295 2295 Size: 5605 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3796 3830 1903 Size: 34 50 34 1775 34 3678 Fragments arranged by size: 3678 1775 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2412 5600 2412 Size: 3188 2417 BlpI GC'TnA_GC Cuts at: 2462 3022 2462 Size: 560 5045 Bme1580I G_kGCm'C Cuts at: 1790 2188 3666 4822 1790 Size: 398 1478 1156 2573 Fragments arranged by size: 2573 1478 1156 398 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2780 4265 1239 Size: 1109 432 1485 2579 Fragments arranged by size: 2579 1485 1109 432 Bpu10I CC'TnA_GC Cuts at: 2295 2295 Size: 5605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2518 2591 4268 882 Size: 617 1019 73 1677 2219 Fragments arranged by size: 2219 1677 1019 617 73 BsaAI yAC'GTr Cuts at: 493 1204 2191 2284 3396 493 Size: 711 987 93 1112 2702 Fragments arranged by size: 2702 1112 987 711 93 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 2167 2343 3412 Size: 53 83 186 936 567 67 176 1069 Cuts at: 3412 3794 4429 275 Size: 382 635 1451 Fragments arranged by size: 1451 1069 936 635 567 382 186 176 83 67 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2875 1186 Size: 1689 3916 BseYI C'CCAG_C Cuts at: 2449 2548 2581 4808 5304 2449 Size: 99 33 2227 496 2750 Fragments arranged by size: 2750 2227 496 99 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2380 2789 1163 Size: 573 644 409 3979 Fragments arranged by size: 3979 644 573 409 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2334 2393 2794 3666 3751 4822 2334 Size: 59 401 872 85 1071 3117 Fragments arranged by size: 3117 1071 872 401 85 59 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2334 2393 2794 2924 3666 3751 Size: 399 398 146 59 401 130 742 85 Cuts at: 3751 4822 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 401 399 398 146 130 85 59 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2520 2825 4100 4274 65 Size: 1183 1272 305 1275 174 1396 Fragments arranged by size: 1396 1275 1272 1183 305 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 2277 794 Size: 1483 4122 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2412 2424 2935 3302 3687 3704 5156 5242 Size: 440 12 511 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 511 440 385 367 346 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2121 2232 513 Size: 552 1056 111 3886 Fragments arranged by size: 3886 1056 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 487 Size: 683 56 917 3949 Fragments arranged by size: 3949 917 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2316 3052 Size: 54 1425 50 275 18 405 81 736 Cuts at: 3052 3941 5570 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 736 405 275 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2521 3535 1353 Size: 1168 1014 3423 Fragments arranged by size: 3423 1168 1014 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2653 2780 3668 4265 5052 5320 Size: 393 716 305 127 888 597 787 268 Cuts at: 5320 1239 Size: 1524 Fragments arranged by size: 1524 888 787 716 597 393 305 268 127 EcoNI CCTnn'n_nnAGG Cuts at: 2053 2382 2053 Size: 329 5276 EcoO109I rG'GnC_Cy Cuts at: 2184 2323 2184 Size: 139 5466 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2549 2579 4752 1678 Size: 871 30 2173 2531 Fragments arranged by size: 2531 2173 871 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373 Size: 32 135 32 2674 32 42 32 2626 Fragments arranged by size: 2674 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 2374 2814 2949 3195 669 Size: 988 281 409 27 440 135 246 3079 Fragments arranged by size: 3079 988 440 409 281 246 135 27 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 4903 1305 Size: 3414 184 2007 Fragments arranged by size: 3414 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 2318 10 Size: 54 1425 343 405 81 3297 Fragments arranged by size: 3297 1425 405 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2049 2699 2830 3563 3922 4081 518 Size: 648 883 650 131 733 359 159 2042 Fragments arranged by size: 2042 883 733 650 648 359 159 131 MspA1I CmG'CkG Cuts at: 1606 2311 2739 3021 3698 4846 5091 1606 Size: 705 428 282 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 428 282 245 NcoI C'CATG_G Cuts at: 513 1065 2232 513 Size: 552 1167 3886 Fragments arranged by size: 3886 1167 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2647 2647 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PmlI CAC'GTG Cuts at: 2191 2284 2191 Size: 93 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 2532 3175 2532 Size: 643 4962 PspOMI G'GGCC_C Cuts at: 2184 2184 Size: 5605 PstI C_TGCA'G Cuts at: 830 2247 2744 2957 830 Size: 1417 497 213 3478 Fragments arranged by size: 3478 1417 497 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2311 2739 2311 Size: 428 5177 SalI G'TCGA_C Cuts at: 1936 2345 2947 1936 Size: 409 602 4594 Fragments arranged by size: 4594 602 409 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2243 2740 2953 4088 4769 4960 826 Size: 219 1198 497 213 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 681 497 219 213 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2024 2177 2932 1872 Size: 152 153 755 4545 Fragments arranged by size: 4545 755 153 152 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2232 2319 2619 3044 513 Size: 552 1167 87 300 425 3074 Fragments arranged by size: 3074 1167 552 425 300 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 3632 3649 3802 3987 4432 325 Size: 1255 960 1092 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1092 960 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2022 2175 2930 1870 Size: 152 153 755 4545 Fragments arranged by size: 4545 755 153 152 XmnI GAAnn'nnTTC Cuts at: 2719 3734 2719 Size: 1015 4590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2168 3413 276 Size: 53 83 186 936 634 1245 2468 Fragments arranged by size: 2468 1245 936 634 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI