(Linear) (Six-base) MAPSORT of: KIEE2722.seq Check: 2665 from: 1 to: 900 KIEE2722.orf With 182 enzymes: SgfI * June 6, 2018 17:50 .. AatII G_ACGT'C Cuts at: 0 165 900 Size: 165 735 AccI GT'mk_AC Cuts at: 0 341 551 900 Size: 341 210 349 Fragments arranged by size: 349 341 210 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 900 Size: 648 252 AfeI AGC'GCT Cuts at: 0 572 900 Size: 572 328 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 424 456 900 Size: 424 32 444 Fragments arranged by size: 444 424 32 AlwNI CAG_nnn'CTG Cuts at: 0 468 900 Size: 468 432 ApaI G_GGCC'C Cuts at: 0 183 900 Size: 183 717 AvaI C'yCGr_G Cuts at: 0 17 170 900 Size: 17 153 730 Fragments arranged by size: 730 153 17 BanII G_rGCy'C Cuts at: 0 183 900 Size: 183 717 BbvCI CC'TCA_GC Cuts at: 0 290 900 Size: 290 610 BglII A'GATC_T Cuts at: 0 407 900 Size: 407 493 BlpI GC'TnA_GC Cuts at: 0 457 900 Size: 457 443 Bme1580I G_kGCm'C Cuts at: 0 183 900 Size: 183 717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 775 900 Size: 343 432 125 Fragments arranged by size: 432 343 125 Bpu10I CC'TnA_GC Cuts at: 0 290 900 Size: 290 610 BsaI GGTCTCn'nnnn_ Cuts at: 0 513 586 900 Size: 513 73 314 Fragments arranged by size: 513 314 73 BsaAI yAC'GTr Cuts at: 0 186 279 900 Size: 186 93 621 Fragments arranged by size: 621 186 93 BsaHI Gr'CG_yC Cuts at: 0 95 162 338 900 Size: 95 67 176 562 Fragments arranged by size: 562 176 95 67 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 870 900 Size: 870 30 BseYI C'CCAG_C Cuts at: 0 444 543 576 900 Size: 444 99 33 324 Fragments arranged by size: 444 324 99 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 375 784 900 Size: 375 409 116 Fragments arranged by size: 409 375 116 BsiHKAI G_wGCw'C Cuts at: 0 329 388 789 900 Size: 329 59 401 111 Fragments arranged by size: 401 329 111 59 Bsp1286I G_dGCh'C Cuts at: 0 183 329 388 789 900 Size: 183 146 59 401 111 Fragments arranged by size: 401 183 146 111 59 BsrDI GCAATG_nn' Cuts at: 0 515 820 900 Size: 515 305 80 Fragments arranged by size: 515 305 80 BstAPI GCAn_nnn'nTGC Cuts at: 0 272 900 Size: 272 628 BstXI CCAn_nnnn'nTGG Cuts at: 0 321 900 Size: 321 579 BstYI r'GATC_y Cuts at: 0 407 419 900 Size: 407 12 481 Fragments arranged by size: 481 407 12 BtgI C'CryG_G Cuts at: 0 116 227 900 Size: 116 111 673 Fragments arranged by size: 673 116 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 138 900 Size: 138 762 EaeI y'GGCC_r Cuts at: 0 230 311 900 Size: 230 81 589 Fragments arranged by size: 589 230 81 EarI CTCTTCn'nnn_ Cuts at: 0 516 900 Size: 516 384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 648 775 900 Size: 343 305 127 125 Fragments arranged by size: 343 305 127 125 EcoNI CCTnn'n_nnAGG Cuts at: 0 48 377 900 Size: 48 329 523 Fragments arranged by size: 523 329 48 EcoO109I rG'GnC_Cy Cuts at: 0 179 318 900 Size: 179 139 582 Fragments arranged by size: 582 179 139 HaeII r_GCGC'y Cuts at: 0 544 574 900 Size: 544 30 326 Fragments arranged by size: 544 326 30 HincII GTy'rAC Cuts at: 0 342 369 809 900 Size: 342 27 440 91 Fragments arranged by size: 440 342 91 27 MscI TGG'CCA Cuts at: 0 232 313 900 Size: 232 81 587 Fragments arranged by size: 587 232 81 MslI CAynn'nnrTG Cuts at: 0 44 694 825 900 Size: 44 650 131 75 Fragments arranged by size: 650 131 75 44 MspA1I CmG'CkG Cuts at: 0 306 734 900 Size: 306 428 166 Fragments arranged by size: 428 306 166 NcoI C'CATG_G Cuts at: 0 227 900 Size: 227 673 PfoI T'CCnGG_A Cuts at: 0 642 900 Size: 642 258 PmlI CAC'GTG Cuts at: 0 186 279 900 Size: 186 93 621 Fragments arranged by size: 621 186 93 PsiI TTA'TAA Cuts at: 0 527 900 Size: 527 373 PspOMI G'GGCC_C Cuts at: 0 179 900 Size: 179 721 PstI C_TGCA'G Cuts at: 0 242 739 900 Size: 242 497 161 Fragments arranged by size: 497 242 161 PvuII CAG'CTG Cuts at: 0 306 734 900 Size: 306 428 166 Fragments arranged by size: 428 306 166 SalI G'TCGA_C Cuts at: 0 340 900 Size: 340 560 SfcI C'TryA_G Cuts at: 0 238 735 900 Size: 238 497 165 Fragments arranged by size: 497 238 165 SmaI CCC'GGG Cuts at: 0 19 172 900 Size: 19 153 728 Fragments arranged by size: 728 153 19 StyI C'CwwG_G Cuts at: 0 227 314 614 900 Size: 227 87 300 286 Fragments arranged by size: 300 286 227 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 535 900 Size: 535 365 XmaI C'CCGG_G Cuts at: 0 17 170 900 Size: 17 153 730 Fragments arranged by size: 730 153 17 XmnI GAAnn'nnTTC Cuts at: 0 714 900 Size: 714 186 ZraI GAC'GTC Cuts at: 0 163 900 Size: 163 737 Enzymes that do cut: AatII AccI AcuI AfeI AloI AlwNI ApaI AvaI BanII BbvCI BglII BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BstAPI BstXI BstYI BtgI BtgZI EaeI EarI Eco57MI EcoNI EcoO109I HaeII HincII MscI MslI MspA1I NcoI PfoI PmlI PsiI PspOMI PstI PvuII SalI SfcI SmaI StyI TaqII XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI BfrBI BglI BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI