Restriction map for
pFN21AE3388
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII AccI AclI AflII AflIII
AgeI AocI ApaI AscI AspI
BbrPI BbvCI BcgI BfrI BsaAI
BsiWI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI Eco81I EcoNI EcoO65I
EcoRI EcoT22I FseI HpaI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 2563 GGTACC G^GTACC
AccIII 1115 TCCGGA T^CCGGA
AcyI 16 GGCGCC G[GA]^CG[CT]C
AhdI 1660 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 744 GTGCAC G^TGCAC
Aor51HI 608 AGCGCT AGC^GCT
ApaLI 744 GTGCAC G^TGCAC
AseI 2227 ATTAAT AT^TAAT
AsnI 2227 ATTAAT AT^TAAT
Asp718I 2563 GGTACC G^GTACC
AspEI 1660 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3143 TGCGCA TGC^GCA
AvrII 2085 CCTAGG C^CTAGG
BalI 1604 TGGCCA TGG^CCA
BbeI 16 GGCGCC GGCGC^C
BciVI 2981 GTATCC GTATCC(6/5)
BclI 2063 TGATCA T^GATCA
BlnI 2085 CCTAGG C^CTAGG
BsaBI 2709 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BsaI 1763 GAGACC GGTCTC(1/5)
BseAI 1115 TCCGGA T^CCGGA
BsmI 1902 GAATGC GAATGC(1/-1)
BspEI 1115 TCCGGA T^CCGGA
BspMI 2742 GCAGGT ACCTGC(4/8)
BssSI 3355 CTCGTG CACGAG(-5/-1)
DraI 3367 TTTAAA TTT^AAA
EagI 1795 CGGCCG C^GGCCG
Eam1105I 1660 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1795 CGGCCG C^GGCCG
Eco47III 608 AGCGCT AGC^GCT
Eco52I 1795 CGGCCG C^GGCCG
EcoRV 2325 GATATC GAT^ATC
FbaI 2063 TGATCA T^GATCA
FspI 3143 TGCGCA TGC^GCA
HgaI 1340 GCGTC GACGC(5/10)
Hin1I 16 GGCGCC G[GA]^CG[CT]C
HincII 331 GTCAAC GT[CT]^[GA]AC
HindII 331 GTCAAC GT[CT]^[GA]AC
HindIII 226 AAGCTT A^AGCTT
KasI 16 GGCGCC G^GCGCC
KpnI 2563 GGTACC GGTAC^C
MamI 2709 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 492 CAATTG C^AATTG
MluNI 1604 TGGCCA TGG^CCA
MroI 1115 TCCGGA T^CCGGA
MscI 1604 TGGCCA TGG^CCA
MunI 492 CAATTG C^AATTG
NarI 16 GGCGCC GG^CGCC
NspI 2639 GCATGC [GA]CATG^[CT]
PacI 2224 TTAATTAA TTAAT^TAA
PshBI 2227 ATTAAT AT^TAAT
PvuI 659 CGATCG CGAT^CG
SbfI 783 CCTGCAGG CCTGCA^GG
ScaI 1242 AGTACT AGT^ACT
SfoI 16 GGCGCC GGC^GCC
SphI 2639 GCATGC GCATG^C
Sse8387I 783 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 513 CGCG CG^CG
2350 CGCG
AcsI 592 AAATTT [GA]^AATT[CT]
3346 AAATTC
ApoI 592 AAATTT [GA]^AATT[CT]
3346 AAATTC
Asp700I 1533 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3126 GAATAACTTC
BamHI 3192 GGATCC G^GATCC
3590 GGATCC
BanI 16 GGCGCC G^G[CT][GA]CC
2563 GGTACC
BfaI 2086 CTAG C^TAG
2263 CTAG
BglII 1289 AGATCT A^GATCT
2708 AGATCT
BlpI 1256 GCTCAGC GC^T[ACGT]AGC
3612 GCTGAGC
Bpu1102I 1256 GCTCAGC GC^T[ACGT]AGC
3612 GCTGAGC
BsaWI 1115 TCCGGA [AT]^CCGG[AT]
1759 ACCGGA
BspHI 1061 TCATGA T^CATGA
2288 TCATGA
BsrBI 1213 CCGCTC CCGCTC(-3/-3)
3538 GAGCGG
BstUI 513 CGCG CG^CG
2350 CGCG
CelII 1256 GCTCAGC GC^T[ACGT]AGC
3612 GCTGAGC
MaeI 2086 CTAG C^TAG
2263 CTAG
MvnI 513 CGCG CG^CG
2350 CGCG
NcoI 815 CCATGG C^CATGG
3056 CCATGG
PaeR7I 1814 CTCGAG C^TCGAG
2657 CTCGAG
RcaI 1061 TCATGA T^CATGA
2288 TCATGA
XhoI 1814 CTCGAG C^TCGAG
2657 CTCGAG
XmnI 1533 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3126 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII