(Circular) (Six-base) MAPSORT of: pFN21AE3388.seq Check: 478 from: 1 to: 8347 pFN21AE3388 8347 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4738 4738 Size: 8347 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6157 278 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5690 1937 Size: 3753 4594 Acc65I G'GTAC_C Cuts at: 4568 5668 4568 Size: 1100 7247 AclI AA'CG_TT Cuts at: 1563 6474 6847 1563 Size: 4911 373 3063 Fragments arranged by size: 4911 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3391 3747 3872 4493 4871 5122 6410 7794 Size: 1759 356 125 621 378 251 1288 1384 Cuts at: 7794 8062 1632 Size: 268 1917 Fragments arranged by size: 1917 1759 1384 1288 621 378 356 268 251 125 AfeI AGC'GCT Cuts at: 2615 2615 Size: 8347 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8150 AflIII A'CryG_T Cuts at: 1203 7246 8223 1203 Size: 6043 977 1327 Fragments arranged by size: 6043 1327 977 AgeI A'CCGG_T Cuts at: 7178 7178 Size: 8347 AhdI GACnn_n'nnGTC Cuts at: 3670 7076 3670 Size: 3406 4941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2798 3344 3805 1184 Size: 1614 546 461 5726 Fragments arranged by size: 5726 1614 546 461 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5412 5444 5412 Size: 32 8315 AlwNI CAG_nnn'CTG Cuts at: 3753 4819 5029 5481 6090 7662 3753 Size: 1066 210 452 609 1572 4438 Fragments arranged by size: 4438 1572 1066 609 452 210 ApaLI G'TGCA_C Cuts at: 2749 6404 7560 2749 Size: 3655 1156 3536 Fragments arranged by size: 3655 3536 1156 ApoI r'AATT_y Cuts at: 1493 2597 5351 5656 5882 6034 8143 1493 Size: 1104 2754 305 226 152 2109 1697 Fragments arranged by size: 2754 2109 1697 1104 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 4233 6901 160 Size: 872 3201 2668 1606 Fragments arranged by size: 3201 2668 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2090 3243 3819 4662 5672 1870 Size: 73 123 24 1153 576 843 1010 4545 Fragments arranged by size: 4545 1153 1010 843 576 123 73 24 AvrII C'CTAG_G Cuts at: 4090 4090 Size: 8347 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2494 2527 2494 Size: 33 8314 BamHI G'GATC_C Cuts at: 5197 5595 5677 6044 5197 Size: 398 82 367 7500 Fragments arranged by size: 7500 398 367 82 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 4568 5668 7123 618 Size: 325 217 627 234 2547 1100 1455 1842 Fragments arranged by size: 2547 1842 1455 1100 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2091 2103 2460 3263 3590 5103 5146 5298 Size: 700 12 357 803 327 1513 43 152 Cuts at: 5298 5425 5525 5532 5666 1391 Size: 127 100 7 134 4072 Fragments arranged by size: 4072 1513 803 700 357 327 152 134 127 100 43 12 7 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 8347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 2968 3049 3408 3453 3581 4499 Size: 971 266 803 81 359 45 128 918 Cuts at: 4499 4869 928 Size: 370 4406 Fragments arranged by size: 4406 971 918 803 370 359 266 128 81 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6538 6572 1903 Size: 34 4601 34 3678 Fragments arranged by size: 4601 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 6239 7449 4997 Size: 1242 1210 5895 Fragments arranged by size: 5895 1242 1210 BclI T'GATC_A Cuts at: 1548 4068 1548 Size: 2520 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2264 2868 Size: 107 122 71 631 605 337 255 604 Cuts at: 2868 3722 4134 5642 6958 136 Size: 854 412 1508 1316 1525 Fragments arranged by size: 1525 1508 1316 854 631 605 604 412 337 255 122 107 71 BglII A'GATC_T Cuts at: 3294 4713 8342 3294 Size: 1419 3629 3299 Fragments arranged by size: 3629 3299 1419 BlpI GC'TnA_GC Cuts at: 3262 5618 5764 3262 Size: 2356 146 5845 Fragments arranged by size: 5845 2356 146 Bme1580I G_kGCm'C Cuts at: 1790 2753 2864 3986 4813 6408 7564 1790 Size: 963 111 1122 827 1595 1156 2573 Fragments arranged by size: 2573 1595 1156 1122 963 827 111 BmgBI CAC'GTC Cuts at: 1318 2139 2568 1318 Size: 821 429 7097 Fragments arranged by size: 7097 821 429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3260 4497 Size: 416 53 83 399 24 1041 802 1237 Cuts at: 4497 4690 6078 7036 442 Size: 193 1388 958 1753 Fragments arranged by size: 1753 1388 1237 1041 958 802 416 399 193 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8347 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 3845 2122 Size: 1723 6624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4475 4493 4640 5486 5561 7007 1239 Size: 3236 18 147 846 75 1446 2579 Fragments arranged by size: 3236 2579 1446 846 147 75 18 Bpu10I CC'TnA_GC Cuts at: 5252 5252 Size: 8347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3638 5540 6472 7337 7635 3638 Size: 1902 932 865 298 4350 Fragments arranged by size: 4350 1902 932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3762 7010 882 Size: 617 2263 3248 2219 Fragments arranged by size: 3248 2263 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6138 493 Size: 711 4934 2702 Fragments arranged by size: 4934 2702 711 BsaBI GATnn'nnATC Cuts at: 4718 8341 4718 Size: 3623 4724 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 6154 6536 7171 Size: 53 83 186 936 489 4132 382 635 Cuts at: 7171 275 Size: 1451 Fragments arranged by size: 4132 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3120 3764 6780 7178 7452 7599 8010 3120 Size: 644 3016 398 274 147 411 3457 Fragments arranged by size: 3457 3016 644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4907 4937 5412 5442 1178 Size: 30 165 30 3504 30 475 30 4083 Fragments arranged by size: 4083 3504 475 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 3373 3376 3499 3608 3734 3974 4042 Size: 898 1289 3 123 109 126 240 68 Cuts at: 4042 4943 4946 5462 1186 Size: 901 3 516 4071 Fragments arranged by size: 4071 1289 901 898 516 240 126 123 109 68 3 3 BseYI C'CCAG_C Cuts at: 3156 3205 3255 3282 4116 4535 5218 7550 8046 Size: 49 50 27 834 419 683 2332 496 Cuts at: 8046 3156 Size: 3457 Fragments arranged by size: 3457 2332 834 683 496 419 50 49 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 4396 4418 4766 4892 5519 1163 Size: 573 720 1940 22 348 126 627 3991 Fragments arranged by size: 3991 1940 720 627 573 348 126 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2667 3582 3803 5797 6558 7586 Size: 275 186 666 915 221 1994 761 1028 Cuts at: 7586 1540 Size: 2301 Fragments arranged by size: 2301 1994 1028 915 761 666 275 221 186 BsiHKAI G_wGCw'C Cuts at: 2451 2753 3525 3590 3623 4365 4847 5146 5230 Size: 302 772 65 33 742 482 299 84 Cuts at: 5230 5472 5532 6408 6493 7564 2451 Size: 242 60 876 85 1071 3234 Fragments arranged by size: 3234 1071 876 772 742 482 302 299 242 85 84 65 60 33 BsmI GAATG_Cn' Cuts at: 3913 5858 5951 3913 Size: 1945 93 6309 Fragments arranged by size: 6309 1945 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3524 4663 5150 3524 Size: 1139 487 6721 Fragments arranged by size: 6721 1139 487 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2103 2451 2460 2753 2864 3263 Size: 399 301 12 348 9 293 111 399 Cuts at: 3263 3525 3590 3623 3986 4365 4813 4847 5103 Size: 262 65 33 363 379 448 34 256 Cuts at: 5103 5146 5230 5298 5425 5472 5525 5532 5666 Size: 43 84 68 127 47 53 7 134 Cuts at: 5666 6408 6493 7564 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 399 379 363 348 301 293 262 256 134 127 111 85 84 68 65 53 47 43 34 33 12 9 7 BspEI T'CCGG_A Cuts at: 3120 8010 3120 Size: 4890 3457 BspHI T'CATG_A Cuts at: 3066 4293 6236 7966 3066 Size: 1227 1943 1730 3447 Fragments arranged by size: 3447 1943 1730 1227 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4738 5702 844 Size: 3894 964 3489 Fragments arranged by size: 3894 3489 964 BsrBI CCG'CTC Cuts at: 1394 1823 3220 5545 6234 1394 Size: 429 1397 2325 689 3507 Fragments arranged by size: 3507 2325 1397 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2542 2709 3711 6842 7016 65 Size: 1183 1294 167 1002 3131 174 1396 Fragments arranged by size: 3131 1396 1294 1183 1002 174 167 BsrFI r'CCGG_y Cuts at: 1700 1810 6991 7178 1700 Size: 110 5181 187 2869 Fragments arranged by size: 5181 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8347 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8347 BssSI C'ACGA_G Cuts at: 5360 6407 7419 5360 Size: 1047 1012 6288 Fragments arranged by size: 6288 1047 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2541 2757 3753 794 Size: 1747 216 996 5388 Fragments arranged by size: 5388 1747 996 216 BstBI TT'CG_AA Cuts at: 7151 7151 Size: 8347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8347 BstXI CCAn_nnnn'nTGG Cuts at: 3331 4945 5062 3331 Size: 1614 117 6616 Fragments arranged by size: 6616 1614 117 BstYI r'GATC_y Cuts at: 1972 2148 2293 3294 4158 4713 5197 5323 5595 Size: 176 145 1001 864 555 484 126 272 Cuts at: 5595 5677 6044 6429 6446 7898 7984 7996 8342 Size: 82 367 385 17 1452 86 12 346 Cuts at: 8342 1972 Size: 1977 Fragments arranged by size: 1977 1452 1001 864 555 484 385 367 346 272 176 145 126 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2820 2898 5061 5273 513 Size: 552 939 816 78 2163 212 3587 Fragments arranged by size: 3587 2163 939 816 552 212 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3285 4371 487 Size: 683 56 2059 1086 4463 Fragments arranged by size: 4463 2059 1086 683 56 BtsI GCAGTG_nn' Cuts at: 2782 5158 5350 5863 6657 6677 2782 Size: 2376 192 513 794 20 4452 Fragments arranged by size: 4452 2376 794 513 192 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2752 2787 529 Size: 35 2188 35 6089 Fragments arranged by size: 6089 2188 35 35 DraI TTT'AAA Cuts at: 5374 5651 5374 Size: 277 8070 DraIII CAC_nnn'GTG Cuts at: 2757 2835 3499 3979 5274 2757 Size: 78 664 480 1295 5830 Fragments arranged by size: 5830 1295 664 480 78 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7354 809 Size: 507 6038 1802 Fragments arranged by size: 6038 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3513 3609 3800 Size: 54 1425 50 275 18 1683 96 191 Cuts at: 3800 4653 5794 6683 8312 8 Size: 853 1141 889 1629 43 Fragments arranged by size: 1683 1629 1425 1141 889 853 275 191 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3800 5794 1537 Size: 275 1988 1994 4090 Fragments arranged by size: 4090 1994 1988 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3443 5301 5585 6277 1353 Size: 2090 1858 284 692 3423 Fragments arranged by size: 3423 2090 1858 692 284 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2019 2043 2111 4101 5190 5345 Size: 1064 138 430 24 68 1990 1089 155 Cuts at: 5345 6934 7308 7454 387 Size: 1589 374 146 1280 Fragments arranged by size: 1990 1589 1280 1089 1064 430 374 155 146 138 68 24 EcoICRI GAG'CTC Cuts at: 3588 5144 5530 3588 Size: 1556 386 6405 Fragments arranged by size: 6405 1556 386 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3391 3747 3872 4475 4493 4640 4871 Size: 393 1759 356 125 603 18 147 231 Cuts at: 4871 5122 5486 5561 6410 7007 7794 8062 1239 Size: 251 364 75 849 597 787 268 1524 Fragments arranged by size: 1759 1524 849 787 603 597 393 364 356 268 251 231 147 125 75 18 EcoO109I rG'GnC_Cy Cuts at: 2036 3160 4015 4972 5551 5560 2036 Size: 1124 855 957 579 9 4823 Fragments arranged by size: 4823 1124 957 855 579 9 EcoRI G'AATT_C Cuts at: 5656 6034 5656 Size: 378 7969 EcoRV GAT'ATC Cuts at: 4332 4332 Size: 8347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 5515 2395 Size: 3120 5227 FspI TGC'GCA Cuts at: 5150 6853 5150 Size: 1703 6644 HaeII r_GCGC'y Cuts at: 1678 2025 2617 2745 2803 5631 7494 1678 Size: 347 592 128 58 2828 1863 2531 Fragments arranged by size: 2828 2531 1863 592 347 128 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2468 2500 2540 Size: 32 135 32 488 32 376 32 40 Cuts at: 2540 2572 3479 3511 3656 3688 4907 4939 5308 Size: 32 907 32 145 32 1219 32 369 Cuts at: 5308 5340 6988 7020 7062 7094 1373 Size: 32 1648 32 42 32 2626 Fragments arranged by size: 2626 1648 1219 907 488 376 369 145 135 42 40 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 5691 5937 669 Size: 988 281 400 3353 246 3079 Fragments arranged by size: 3353 3079 988 400 281 246 HindIII A'AGCT_T Cuts at: 748 2231 748 Size: 1483 6864 HpaI GTT'AAC Cuts at: 5937 5937 Size: 8347 KasI G'GCGC_C Cuts at: 2021 2021 Size: 8347 KpnI G_GTAC'C Cuts at: 4572 5672 4572 Size: 1100 7247 MfeI C'AATT_G Cuts at: 2497 5946 2497 Size: 3449 4898 MluI A'CGCG_T Cuts at: 8223 8223 Size: 8347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2176 2700 3288 7461 7645 1305 Size: 798 73 524 588 4173 184 2007 Fragments arranged by size: 4173 2007 798 588 524 184 73 MscI TGG'CCA Cuts at: 10 64 1489 1832 3611 10 Size: 54 1425 343 1779 4746 Fragments arranged by size: 4746 1779 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3326 4649 4943 6305 6664 6823 518 Size: 648 2160 1323 294 1362 359 159 2042 Fragments arranged by size: 2160 2042 1362 1323 648 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2943 3205 5194 5218 5763 6440 7588 7833 Size: 1337 262 1989 24 545 677 1148 245 Cuts at: 7833 1606 Size: 2120 Fragments arranged by size: 2120 1989 1337 1148 677 545 262 245 24 NarI GG'CG_CC Cuts at: 2022 2022 Size: 8347 NcoI C'CATG_G Cuts at: 513 1065 2004 2820 5061 513 Size: 552 939 816 2241 3799 Fragments arranged by size: 3799 2241 939 816 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8347 NotI GC'GGCC_GC Cuts at: 5794 5794 Size: 8347 NspI r_CATG'y Cuts at: 1132 1229 4648 5705 7250 1132 Size: 97 3419 1057 1545 2229 Fragments arranged by size: 3419 2229 1545 1057 97 PacI TTA_AT'TAA Cuts at: 4233 4233 Size: 8347 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8347 PciI A'CATG_T Cuts at: 7246 7246 Size: 8347 PflMI CCAn_nnn'nTGG Cuts at: 1108 2719 3298 4560 1108 Size: 1611 579 1262 4895 Fragments arranged by size: 4895 1611 1262 579 PfoI T'CCnGG_A Cuts at: 5118 5118 Size: 8347 PmeI GTTT'AAAC Cuts at: 5651 5651 Size: 8347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5412 5444 6416 6448 7929 7961 5412 Size: 32 972 32 1481 32 5798 Fragments arranged by size: 5798 1481 972 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 3160 4015 4972 5551 5560 2036 Size: 1124 855 957 579 9 4823 Fragments arranged by size: 4823 1124 957 855 579 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8347 PsiI TTA'TAA Cuts at: 5917 5917 Size: 8347 PstI C_TGCA'G Cuts at: 830 2793 5111 5193 5699 830 Size: 1963 2318 82 506 3478 Fragments arranged by size: 3478 2318 1963 506 82 PvuI CG_AT'CG Cuts at: 2001 2667 2001 Size: 666 7681 PvuII CAG'CTG Cuts at: 2943 3205 5194 5218 2943 Size: 262 1989 24 6072 Fragments arranged by size: 6072 1989 262 24 SacI G_AGCT'C Cuts at: 3590 5146 5532 3590 Size: 1556 386 6405 Fragments arranged by size: 6405 1556 386 SalI G'TCGA_C Cuts at: 1936 5689 1936 Size: 3753 4594 SanDI GG'GwC_CC Cuts at: 2036 4015 2036 Size: 1979 6368 SbfI CC_TGCA'GG Cuts at: 2793 5699 2793 Size: 2906 5441 ScaI AGT'ACT Cuts at: 3249 6595 8307 3249 Size: 3346 1712 3289 Fragments arranged by size: 3346 3289 1712 SfcI C'TryA_G Cuts at: 826 1045 2789 4676 5107 5189 5695 6830 7511 Size: 219 1744 1887 431 82 506 1135 681 Cuts at: 7511 7702 826 Size: 191 1471 Fragments arranged by size: 1887 1744 1471 1135 681 506 431 219 191 82 SfoI GGC'GCC Cuts at: 2023 2023 Size: 8347 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8347 SmaI CCC'GGG Cuts at: 1872 5674 1872 Size: 3802 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3653 3819 4662 5555 6451 7352 Size: 197 926 1710 166 843 893 896 901 Cuts at: 7352 7614 820 Size: 262 1553 Fragments arranged by size: 1710 1553 926 901 896 893 843 262 197 166 SnaBI TAC'GTA Cuts at: 493 6138 493 Size: 5645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8347 SphI G_CATG'C Cuts at: 1132 4648 5705 1132 Size: 3516 1057 3774 Fragments arranged by size: 3774 3516 1057 SspI AAT'ATT Cuts at: 5 52 6271 5 Size: 47 6219 2081 Fragments arranged by size: 6219 2081 47 StuI AGG'CCT Cuts at: 1517 3380 3566 3680 4763 1517 Size: 1863 186 114 1083 5101 Fragments arranged by size: 5101 1863 1083 186 114 StyI C'CwwG_G Cuts at: 513 1065 2004 2820 3660 4020 4090 4572 4723 Size: 552 939 816 840 360 70 482 151 Cuts at: 4723 5061 5574 5786 513 Size: 338 513 212 3074 Fragments arranged by size: 3074 939 840 816 552 513 482 360 338 212 151 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 2911 3296 3596 4380 4955 5259 Size: 1255 897 434 385 300 784 575 304 Cuts at: 5259 6374 6391 6544 6729 7174 325 Size: 1115 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1115 897 784 575 445 434 385 304 300 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 3247 6593 Size: 274 80 33 51 1443 489 781 3346 Cuts at: 6593 8305 96 Size: 1712 138 Fragments arranged by size: 3346 1712 1443 781 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2564 2596 5587 5619 8307 8339 2564 Size: 32 2991 32 2688 32 2572 Fragments arranged by size: 2991 2688 2572 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8347 XbaI T'CTAG_A Cuts at: 5683 5683 Size: 8347 XhoI C'TCGA_G Cuts at: 1943 3819 4662 1943 Size: 1876 843 5628 Fragments arranged by size: 5628 1876 843 XmaI C'CCGG_G Cuts at: 1870 5672 1870 Size: 3802 4545 XmnI GAAnn'nnTTC Cuts at: 3542 5135 6476 3542 Size: 1593 1341 5413 Fragments arranged by size: 5413 1593 1341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6155 276 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI