Restriction map for
pFN21AE3388
(Linear) (Six-base) MAPSORT of: KIEE3388.seq Check: 6752 from: 1 to: 3642
KIEE3388.orf
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2733 3642
Size: 2733 909
Acc65I G'GTAC_C
Cuts at: 0 2563 3642
Size: 2563 1079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1742 1867 2488 2866 3117 3642
Size: 1386 356 125 621 378 251 525
Fragments arranged by size:
1386 621 525 378 356 251 125
AfeI AGC'GCT
Cuts at: 0 610 3642
Size: 610 3032
AhdI GACnn_n'nnGTC
Cuts at: 0 1665 3642
Size: 1665 1977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 1339 1800 3642
Size: 793 546 461 1842
Fragments arranged by size:
1842 793 546 461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3407 3439 3642
Size: 3407 32 203
Fragments arranged by size:
3407 203 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1748 2814 3024 3476 3642
Size: 1748 1066 210 452 166
Fragments arranged by size:
1748 1066 452 210 166
ApaLI G'TGCA_C
Cuts at: 0 744 3642
Size: 744 2898
ApoI r'AATT_y
Cuts at: 0 592 3346 3642
Size: 592 2754 296
Fragments arranged by size:
2754 592 296
AseI AT'TA_AT
Cuts at: 0 2228 3642
Size: 2228 1414
AvaI C'yCGr_G
Cuts at: 0 61 85 1238 1814 2657 3642
Size: 61 24 1153 576 843 985
Fragments arranged by size:
1153 985 843 576 61 24
AvrII C'CTAG_G
Cuts at: 0 2085 3642
Size: 2085 1557
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 489 522 3642
Size: 489 33 3120
Fragments arranged by size:
3120 489 33
BamHI G'GATC_C
Cuts at: 0 3192 3590 3642
Size: 3192 398 52
Fragments arranged by size:
3192 398 52
BanI G'GyrC_C
Cuts at: 0 16 2563 3642
Size: 16 2547 1079
Fragments arranged by size:
2547 1079 16
BanII G_rGCy'C
Cuts at: 0 86 98 455 1258 1585 3098 3141 3293
Size: 86 12 357 803 327 1513 43 152
Cuts at: 3293 3420 3520 3527 3642
Size: 127 100 7 115
Fragments arranged by size:
1513 803 357 327 152 127 115 100
86 43 12 7
BbeI G_GCGC'C
Cuts at: 0 20 3642
Size: 20 3622
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 963 1044 1403 1448 1576 2494 2864
Size: 160 803 81 359 45 128 918 370
Cuts at: 2864 3642
Size: 778
Fragments arranged by size:
918 803 778 370 359 160 128 81
45
BciVI GTATCCnnnnn_n'
Cuts at: 0 2992 3642
Size: 2992 650
BclI T'GATC_A
Cuts at: 0 2063 3642
Size: 2063 1579
BglI GCCn_nnn'nGGC
Cuts at: 0 259 863 1717 2129 3637 3642
Size: 259 604 854 412 1508 5
Fragments arranged by size:
1508 854 604 412 259 5
BglII A'GATC_T
Cuts at: 0 1289 2708 3642
Size: 1289 1419 934
Fragments arranged by size:
1419 1289 934
BlpI GC'TnA_GC
Cuts at: 0 1257 3613 3642
Size: 1257 2356 29
Fragments arranged by size:
2356 1257 29
Bme1580I G_kGCm'C
Cuts at: 0 748 859 1981 2808 3642
Size: 748 111 1122 827 834
Fragments arranged by size:
1122 834 827 748 111
BmgBI CAC'GTC
Cuts at: 0 134 563 3642
Size: 134 429 3079
Fragments arranged by size:
3079 429 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 1255 2492 2685 3642
Size: 453 802 1237 193 957
Fragments arranged by size:
1237 957 802 453 193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1840 3642
Size: 117 1723 1802
Fragments arranged by size:
1802 1723 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2470 2488 2635 3481 3556 3642
Size: 2470 18 147 846 75 86
Fragments arranged by size:
2470 846 147 86 75 18
Bpu10I CC'TnA_GC
Cuts at: 0 3247 3642
Size: 3247 395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 3535 3642
Size: 1633 1902 107
Fragments arranged by size:
1902 1633 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1757 3642
Size: 1757 1885
BsaBI GATnn'nnATC
Cuts at: 0 2713 3642
Size: 2713 929
BsaHI Gr'CG_yC
Cuts at: 0 17 3642
Size: 17 3625
BsaWI w'CCGG_w
Cuts at: 0 1115 1759 3642
Size: 1115 644 1883
Fragments arranged by size:
1883 1115 644
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2902 2932 3407 3437 3642
Size: 2902 30 475 30 205
Fragments arranged by size:
2902 475 205 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 1368 1371 1494 1603 1729 1969 2037
Size: 79 1289 3 123 109 126 240 68
Cuts at: 2037 2938 2941 3457 3642
Size: 901 3 516 185
Fragments arranged by size:
1289 901 516 240 185 126 123 109
79 68 3 3
BseYI C'CCAG_C
Cuts at: 0 1151 1200 1250 1277 2111 2530 3213 3642
Size: 1151 49 50 27 834 419 683 429
Fragments arranged by size:
1151 834 683 429 419 50 49 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 2391 2413 2761 2887 3514 3642
Size: 451 1940 22 348 126 627 128
Fragments arranged by size:
1940 627 451 348 128 126 22
BsiEI CG_ry'CG
Cuts at: 0 662 1577 1798 3642
Size: 662 915 221 1844
Fragments arranged by size:
1844 915 662 221
BsiHKAI G_wGCw'C
Cuts at: 0 446 748 1520 1585 1618 2360 2842 3141
Size: 446 302 772 65 33 742 482 299
Cuts at: 3141 3225 3467 3527 3642
Size: 84 242 60 115
Fragments arranged by size:
772 742 482 446 302 299 242 115
84 65 60 33
BsmI GAATG_Cn'
Cuts at: 0 1908 3642
Size: 1908 1734
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1519 2658 3145 3642
Size: 1519 1139 487 497
Fragments arranged by size:
1519 1139 497 487
Bsp1286I G_dGCh'C
Cuts at: 0 86 98 446 455 748 859 1258 1520
Size: 86 12 348 9 293 111 399 262
Cuts at: 1520 1585 1618 1981 2360 2808 2842 3098 3141
Size: 65 33 363 379 448 34 256 43
Cuts at: 3141 3225 3293 3420 3467 3520 3527 3642
Size: 84 68 127 47 53 7 115
Fragments arranged by size:
448 399 379 363 348 293 262 256
127 115 111 86 84 68 65 53
47 43 34 33 12 9 7
BspEI T'CCGG_A
Cuts at: 0 1115 3642
Size: 1115 2527
BspHI T'CATG_A
Cuts at: 0 1061 2288 3642
Size: 1061 1227 1354
Fragments arranged by size:
1354 1227 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2733 3642
Size: 2733 909
BsrBI CCG'CTC
Cuts at: 0 1215 3540 3642
Size: 1215 2325 102
Fragments arranged by size:
2325 1215 102
BsrDI GCAATG_nn'
Cuts at: 0 537 704 1706 3642
Size: 537 167 1002 1936
Fragments arranged by size:
1936 1002 537 167
BssSI C'ACGA_G
Cuts at: 0 3355 3642
Size: 3355 287
BstAPI GCAn_nnn'nTGC
Cuts at: 0 536 752 1748 3642
Size: 536 216 996 1894
Fragments arranged by size:
1894 996 536 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1326 2940 3057 3642
Size: 1326 1614 117 585
Fragments arranged by size:
1614 1326 585 117
BstYI r'GATC_y
Cuts at: 0 143 288 1289 2153 2708 3192 3318 3590
Size: 143 145 1001 864 555 484 126 272
Cuts at: 3590 3642
Size: 52
Fragments arranged by size:
1001 864 555 484 272 145 143 126
52
BtgI C'CryG_G
Cuts at: 0 815 893 3056 3268 3642
Size: 815 78 2163 212 374
Fragments arranged by size:
2163 815 374 212 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1280 2366 3642
Size: 1280 1086 1276
Fragments arranged by size:
1280 1276 1086
BtsI GCAGTG_nn'
Cuts at: 0 777 3153 3345 3642
Size: 777 2376 192 297
Fragments arranged by size:
2376 777 297 192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 747 782 3642
Size: 747 35 2860
Fragments arranged by size:
2860 747 35
DraI TTT'AAA
Cuts at: 0 3369 3642
Size: 3369 273
DraIII CAC_nnn'GTG
Cuts at: 0 752 830 1494 1974 3269 3642
Size: 752 78 664 480 1295 373
Fragments arranged by size:
1295 752 664 480 373 78
EaeI y'GGCC_r
Cuts at: 0 1508 1604 1795 2648 3642
Size: 1508 96 191 853 994
Fragments arranged by size:
1508 994 853 191 96
EagI C'GGCC_G
Cuts at: 0 1795 3642
Size: 1795 1847
EarI CTCTTCn'nnn_
Cuts at: 0 1438 3296 3580 3642
Size: 1438 1858 284 62
Fragments arranged by size:
1858 1438 284 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 14 38 106 2096 3185 3340 3642
Size: 14 24 68 1990 1089 155 302
Fragments arranged by size:
1990 1089 302 155 68 24 14
EcoICRI GAG'CTC
Cuts at: 0 1583 3139 3525 3642
Size: 1583 1556 386 117
Fragments arranged by size:
1583 1556 386 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1742 1867 2470 2488 2635 2866 3117
Size: 1386 356 125 603 18 147 231 251
Cuts at: 3117 3481 3556 3642
Size: 364 75 86
Fragments arranged by size:
1386 603 364 356 251 231 147 125
86 75 18
EcoO109I rG'GnC_Cy
Cuts at: 0 31 1155 2010 2967 3546 3555 3642
Size: 31 1124 855 957 579 9 87
Fragments arranged by size:
1124 957 855 579 87 31 9
EcoRV GAT'ATC
Cuts at: 0 2327 3642
Size: 2327 1315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 3510 3642
Size: 390 3120 132
Fragments arranged by size:
3120 390 132
FspI TGC'GCA
Cuts at: 0 3145 3642
Size: 3145 497
HaeII r_GCGC'y
Cuts at: 0 20 612 740 798 3626 3642
Size: 20 592 128 58 2828 16
Fragments arranged by size:
2828 592 128 58 20 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 463 495 535 567 1474 1506
Size: 55 32 376 32 40 32 907 32
Cuts at: 1506 1651 1683 2902 2934 3303 3335 3642
Size: 145 32 1219 32 369 32 307
Fragments arranged by size:
1219 907 376 369 307 145 55 40
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 333 3642
Size: 333 3309
HindIII A'AGCT_T
Cuts at: 0 226 3642
Size: 226 3416
KasI G'GCGC_C
Cuts at: 0 16 3642
Size: 16 3626
KpnI G_GTAC'C
Cuts at: 0 2567 3642
Size: 2567 1075
MfeI C'AATT_G
Cuts at: 0 492 3642
Size: 492 3150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 171 695 1283 3642
Size: 98 73 524 588 2359
Fragments arranged by size:
2359 588 524 98 73
MscI TGG'CCA
Cuts at: 0 1606 3642
Size: 1606 2036
MslI CAynn'nnrTG
Cuts at: 0 1321 2644 2938 3642
Size: 1321 1323 294 704
Fragments arranged by size:
1323 1321 704 294
MspA1I CmG'CkG
Cuts at: 0 938 1200 3189 3213 3642
Size: 938 262 1989 24 429
Fragments arranged by size:
1989 938 429 262 24
NarI GG'CG_CC
Cuts at: 0 17 3642
Size: 17 3625
NcoI C'CATG_G
Cuts at: 0 815 3056 3642
Size: 815 2241 586
Fragments arranged by size:
2241 815 586
NspI r_CATG'y
Cuts at: 0 2643 3642
Size: 2643 999
PacI TTA_AT'TAA
Cuts at: 0 2228 3642
Size: 2228 1414
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 1293 2555 3642
Size: 714 579 1262 1087
Fragments arranged by size:
1262 1087 714 579
PfoI T'CCnGG_A
Cuts at: 0 3113 3642
Size: 3113 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3407 3439 3642
Size: 3407 32 203
Fragments arranged by size:
3407 203 32
PpuMI rG'GwC_Cy
Cuts at: 0 31 1155 2010 2967 3546 3555 3642
Size: 31 1124 855 957 579 9 87
Fragments arranged by size:
1124 957 855 579 87 31 9
PstI C_TGCA'G
Cuts at: 0 788 3106 3188 3642
Size: 788 2318 82 454
Fragments arranged by size:
2318 788 454 82
PvuI CG_AT'CG
Cuts at: 0 662 3642
Size: 662 2980
PvuII CAG'CTG
Cuts at: 0 938 1200 3189 3213 3642
Size: 938 262 1989 24 429
Fragments arranged by size:
1989 938 429 262 24
SacI G_AGCT'C
Cuts at: 0 1585 3141 3527 3642
Size: 1585 1556 386 115
Fragments arranged by size:
1585 1556 386 115
SanDI GG'GwC_CC
Cuts at: 0 31 2010 3642
Size: 31 1979 1632
Fragments arranged by size:
1979 1632 31
SbfI CC_TGCA'GG
Cuts at: 0 788 3642
Size: 788 2854
ScaI AGT'ACT
Cuts at: 0 1244 3642
Size: 1244 2398
SfcI C'TryA_G
Cuts at: 0 784 2671 3102 3184 3642
Size: 784 1887 431 82 458
Fragments arranged by size:
1887 784 458 431 82
SfoI GGC'GCC
Cuts at: 0 18 3642
Size: 18 3624
SmlI C'TyrA_G
Cuts at: 0 1648 1814 2657 3550 3642
Size: 1648 166 843 893 92
Fragments arranged by size:
1648 893 843 166 92
SphI G_CATG'C
Cuts at: 0 2643 3642
Size: 2643 999
StuI AGG'CCT
Cuts at: 0 1375 1561 1675 2758 3642
Size: 1375 186 114 1083 884
Fragments arranged by size:
1375 1083 884 186 114
StyI C'CwwG_G
Cuts at: 0 815 1655 2015 2085 2567 2718 3056 3569
Size: 815 840 360 70 482 151 338 513
Cuts at: 3569 3642
Size: 73
Fragments arranged by size:
840 815 513 482 360 338 151 73
70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 906 1291 1591 2375 2950 3254 3642
Size: 472 434 385 300 784 575 304 388
Fragments arranged by size:
784 575 472 434 388 385 304 300
TatI w'GTAC_w
Cuts at: 0 461 1242 3642
Size: 461 781 2400
Fragments arranged by size:
2400 781 461
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 3582 3614 3642
Size: 559 32 2991 32 28
Fragments arranged by size:
2991 559 32 32 28
XhoI C'TCGA_G
Cuts at: 0 1814 2657 3642
Size: 1814 843 985
Fragments arranged by size:
1814 985 843
XmnI GAAnn'nnTTC
Cuts at: 0 1537 3130 3642
Size: 1537 1593 512
Fragments arranged by size:
1593 1537 512
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I
NarI NcoI NspI PacI PflMI PfoI PpiI PpuMI
PstI PvuI PvuII SacI SanDI SbfI ScaI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AleI ApaI
AscI AsiSI BbvCI BcgI BfrBI BmtI BsaAI BsiWI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
DrdI EcoNI EcoRI FseI FspAI HpaI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI RsrII
SacII SalI SapI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI
XmaI ZraI