################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BanII BbrPI BcgI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsiEI BsmI BspDI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 718 GACGTC GACGT^C AccI 1222 GTCTAC GT^[AC][GT]AC AcsI 2350 GAATTC [GA]^AATT[CT] AflIII 1316 ACATGT A^C[GA][CT]GT AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 265 CCTCAGG CC^T[ACGT]AGG ApoI 2350 GAATTC [GA]^AATT[CT] AvrII 1452 CCTAGG C^CTAGG BamHI 563 GGATCC G^GATCC BbvCI 2130 GCTGAGG CCTCAGC(-5/-2) BciVI 1947 GTATCC GTATCC(6/5) BfaI 1453 CTAG C^TAG BglI 733 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1452 CCTAGG C^CTAGG BsiWI 1142 CGTACG C^GTACG Bsp1407I 1721 TGTACA T^GTACA BspHI 1730 TCATGA T^CATGA BspLU11I 1316 ACATGT A^CATGT BsrBI 670 GAGCGG CCGCTC(-3/-3) BsrGI 1721 TGTACA T^GTACA Bsu36I 265 CCTCAGG CC^T[ACGT]AGG Eco81I 265 CCTCAGG CC^T[ACGT]AGG EcoNI 513 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2350 GAATTC G^AATTC EcoT22I 2330 ATGCAT ATGCA^T HincII 721 GTCAAC GT[CT]^[GA]AC HindII 721 GTCAAC GT[CT]^[GA]AC MaeI 1453 CTAG C^TAG NdeI 1288 CATATG CA^TATG NsiI 2330 ATGCAT ATGCA^T PflMI 1707 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1730 TCATGA T^CATGA SapI 328 GAAGAGC GCTCTTC(1/4) SgrAI 708 CGCCGGCG C[GA]^CCGG[CT]G SphI 808 GCATGC GCATG^C SspBI 1721 TGTACA T^GTACA Tsp509I 2351 AATT ^AATT Van91I 1707 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 1109 TCCGGA T^CCGGA 2144 TCCGGA Alw44I 119 GTGCAC G^TGCAC 311 GTGCAC ApaLI 119 GTGCAC G^TGCAC 311 GTGCAC AvaII 1147 GGACC G^G[AT]CC 2093 GGACC BalI 320 TGGCCA TGG^CCA 1658 TGGCCA BsaWI 1109 TCCGGA [AT]^CCGG[AT] 2144 TCCGGA BseAI 1109 TCCGGA T^CCGGA 2144 TCCGGA BsgI 1273 CTGCAC GTGCAG(16/14) 832 GTGCAG BspEI 1109 TCCGGA T^CCGGA 2144 TCCGGA BspMI 508 ACCTGC ACCTGC(4/8) 602 GCAGGT BsrDI 1506 CATTGC GCAATG(2/0) 2019 CATTGC BstAPI 1248 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1275 GCACACGCTGC BstEII 909 GGTGACC G^GT[ACGT]ACC 1918 GGTGACC BstPI 909 GGTGACC G^GT[ACGT]ACC 1918 GGTGACC DraII 739 GGGGCCT [GA]G^G[ACGT]CC[CT] 2259 GGGGCCT EcoO109I 739 GGGGCCT [GA]G^G[ACGT]CC[CT] 2259 GGGGCCT EcoO65I 909 GGTGACC G^GT[ACGT]ACC 1918 GGTGACC MluNI 320 TGGCCA TGG^CCA 1658 TGGCCA MroI 1109 TCCGGA T^CCGGA 2144 TCCGGA MscI 320 TGGCCA TGG^CCA 1658 TGGCCA MseI 529 TTAA T^TAA 2246 TTAA NaeI 90 GCCGGC GCC^GGC 709 GCCGGC NgoMIV 90 GCCGGC G^CCGGC 709 GCCGGC PleI 250 GACTC GAGTC(4/5) 877 GACTC SmaI 1388 CCCGGG CCC^GGG 2256 CCCGGG Tru9I 529 TTAA T^TAA 2246 TTAA XcmI 308 CCAGTGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2090 CCAGGACCATCTTGG XmaCI 1388 CCCGGG C^CCGGG 2256 CCCGGG XmaI 1388 CCCGGG C^CCGGG 2256 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI BanI BbeI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII