(Linear) (Six-base) MAPSORT of: KIEE2371.seq Check: 9058 from: 1 to: 2370 KIEE2371.orf With 182 enzymes: SgfI * October 11, 2011 13:28 .. AatII G_ACGT'C Cuts at: 0 722 2370 Size: 722 1648 AccI GT'mk_AC Cuts at: 0 1223 2370 Size: 1223 1147 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 602 1090 1783 1857 2370 Size: 376 226 488 693 74 513 Fragments arranged by size: 693 513 488 376 226 74 AflIII A'CryG_T Cuts at: 0 1316 2370 Size: 1316 1054 AleI CACnn'nnGTG Cuts at: 0 1180 2370 Size: 1180 1190 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1546 1578 2370 Size: 1546 32 792 Fragments arranged by size: 1546 792 32 AlwNI CAG_nnn'CTG Cuts at: 0 840 2370 Size: 840 1530 ApaLI G'TGCA_C Cuts at: 0 119 311 2370 Size: 119 192 2059 Fragments arranged by size: 2059 192 119 ApoI r'AATT_y Cuts at: 0 2350 2370 Size: 2350 20 AvaI C'yCGr_G Cuts at: 0 823 1388 2256 2370 Size: 823 565 868 114 Fragments arranged by size: 868 823 565 114 AvrII C'CTAG_G Cuts at: 0 1452 2370 Size: 1452 918 BamHI G'GATC_C Cuts at: 0 563 2370 Size: 563 1807 BanI G'GyrC_C Cuts at: 0 24 443 712 2075 2370 Size: 24 419 269 1363 295 Fragments arranged by size: 1363 419 295 269 24 BbeI G_GCGC'C Cuts at: 0 447 716 2079 2370 Size: 447 269 1363 291 Fragments arranged by size: 1363 447 291 269 BbsI GAAGACnn'nnnn_ Cuts at: 0 180 511 1602 2233 2258 2370 Size: 180 331 1091 631 25 112 Fragments arranged by size: 1091 631 331 180 112 25 BbvCI CC'TCA_GC Cuts at: 0 2131 2370 Size: 2131 239 BciVI GTATCCnnnnn_n' Cuts at: 0 1958 2370 Size: 1958 412 BfrBI ATG'CAT Cuts at: 0 2332 2370 Size: 2332 38 BglI GCCn_nnn'nGGC Cuts at: 0 739 2370 Size: 739 1631 Bme1580I G_kGCm'C Cuts at: 0 123 315 2370 Size: 123 192 2055 Fragments arranged by size: 2055 192 123 BmgBI CAC'GTC Cuts at: 0 2235 2370 Size: 2235 135 BmrI ACTGGGnnnn_n' Cuts at: 0 50 252 302 999 1146 2370 Size: 50 202 50 697 147 1224 Fragments arranged by size: 1224 697 202 147 50 50 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2067 2370 Size: 2067 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1018 1285 2370 Size: 154 864 267 1085 Fragments arranged by size: 1085 864 267 154 Bpu10I CC'TnA_GC Cuts at: 0 2131 2370 Size: 2131 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 277 994 1518 2370 Size: 277 717 524 852 Fragments arranged by size: 852 717 524 277 BsaHI Gr'CG_yC Cuts at: 0 444 713 719 790 2076 2370 Size: 444 269 6 71 1286 294 Fragments arranged by size: 1286 444 294 269 71 6 BsaWI w'CCGG_w Cuts at: 0 1109 2144 2370 Size: 1109 1035 226 Fragments arranged by size: 1109 1035 226 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 165 195 235 265 1843 1873 2370 Size: 165 30 40 30 1578 30 497 Fragments arranged by size: 1578 497 165 40 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 170 254 351 412 643 1408 1411 1993 Size: 170 84 97 61 231 765 3 582 Cuts at: 1993 1996 2148 2370 Size: 3 152 222 Fragments arranged by size: 765 582 231 222 170 152 97 84 61 3 3 BseYI C'CCAG_C Cuts at: 0 1673 2370 Size: 1673 697 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 1258 2370 Size: 853 405 1112 Fragments arranged by size: 1112 853 405 BsiHKAI G_wGCw'C Cuts at: 0 123 315 851 1472 2370 Size: 123 192 536 621 898 Fragments arranged by size: 898 621 536 192 123 BsiWI C'GTAC_G Cuts at: 0 1142 2370 Size: 1142 1228 BsmBI CGTCTCn'nnnn_ Cuts at: 0 781 1377 2055 2241 2370 Size: 781 596 678 186 129 Fragments arranged by size: 781 678 596 186 129 Bsp1286I G_dGCh'C Cuts at: 0 123 315 851 1472 2370 Size: 123 192 536 621 898 Fragments arranged by size: 898 621 536 192 123 BspEI T'CCGG_A Cuts at: 0 1109 2144 2370 Size: 1109 1035 226 Fragments arranged by size: 1109 1035 226 BspHI T'CATG_A Cuts at: 0 1730 2370 Size: 1730 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 517 593 2370 Size: 517 76 1777 Fragments arranged by size: 1777 517 76 BsrBI CCG'CTC Cuts at: 0 672 2370 Size: 672 1698 BsrDI GCAATG_nn' Cuts at: 0 1505 2018 2370 Size: 1505 513 352 Fragments arranged by size: 1505 513 352 BsrFI r'CCGG_y Cuts at: 0 90 709 1244 2370 Size: 90 619 535 1126 Fragments arranged by size: 1126 619 535 90 BsrGI T'GTAC_A Cuts at: 0 1721 2370 Size: 1721 649 BssSI C'ACGA_G Cuts at: 0 850 881 1651 2370 Size: 850 31 770 719 Fragments arranged by size: 850 770 719 31 BstAPI GCAn_nnn'nTGC Cuts at: 0 1254 1281 2370 Size: 1254 27 1089 Fragments arranged by size: 1254 1089 27 BstEII G'GTnAC_C Cuts at: 0 909 1918 2370 Size: 909 1009 452 Fragments arranged by size: 1009 909 452 BstXI CCAn_nnnn'nTGG Cuts at: 0 21 201 1668 1914 2370 Size: 21 180 1467 246 456 Fragments arranged by size: 1467 456 246 180 21 BstYI r'GATC_y Cuts at: 0 563 1064 1533 2370 Size: 563 501 469 837 Fragments arranged by size: 837 563 501 469 Bsu36I CC'TnA_GG Cuts at: 0 266 2370 Size: 266 2104 BtgI C'CryG_G Cuts at: 0 200 914 1193 1661 1712 2370 Size: 200 714 279 468 51 658 Fragments arranged by size: 714 658 468 279 200 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 645 672 687 2370 Size: 645 27 15 1683 Fragments arranged by size: 1683 645 27 15 BtsI GCAGTG_nn' Cuts at: 0 226 1889 2370 Size: 226 1663 481 Fragments arranged by size: 1663 481 226 EaeI y'GGCC_r Cuts at: 0 320 917 1040 1658 2168 2370 Size: 320 597 123 618 510 202 Fragments arranged by size: 618 597 510 320 202 123 EarI CTCTTCn'nnn_ Cuts at: 0 79 109 323 578 1900 2370 Size: 79 30 214 255 1322 470 Fragments arranged by size: 1322 470 255 214 79 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 109 1075 2370 Size: 109 966 1295 Fragments arranged by size: 1295 966 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 376 602 1018 1090 1285 1783 1857 Size: 154 222 226 416 72 195 498 74 Cuts at: 1857 2370 Size: 513 Fragments arranged by size: 513 498 416 226 222 195 154 74 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 517 2370 Size: 517 1853 EcoO109I rG'GnC_Cy Cuts at: 0 740 2260 2370 Size: 740 1520 110 Fragments arranged by size: 1520 740 110 EcoRI G'AATT_C Cuts at: 0 2350 2370 Size: 2350 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 384 759 1609 2370 Size: 384 375 850 761 Fragments arranged by size: 850 761 384 375 HaeII r_GCGC'y Cuts at: 0 447 655 716 2079 2370 Size: 447 208 61 1363 291 Fragments arranged by size: 1363 447 291 208 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 12 44 165 197 235 267 274 306 Size: 12 32 121 32 38 32 7 32 Cuts at: 306 1525 1557 1843 1875 2370 Size: 1219 32 286 32 495 Fragments arranged by size: 1219 495 286 121 38 32 32 32 32 32 32 12 7 HincII GTy'rAC Cuts at: 0 723 2370 Size: 723 1647 KasI G'GCGC_C Cuts at: 0 443 712 2075 2370 Size: 443 269 1363 295 Fragments arranged by size: 1363 443 295 269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 233 518 2208 2370 Size: 233 285 1690 162 Fragments arranged by size: 1690 285 233 162 MscI TGG'CCA Cuts at: 0 322 1660 2370 Size: 322 1338 710 Fragments arranged by size: 1338 710 322 MslI CAynn'nnrTG Cuts at: 0 1180 1492 1717 2370 Size: 1180 312 225 653 Fragments arranged by size: 1180 653 312 225 MspA1I CmG'CkG Cuts at: 0 318 837 1032 1038 1272 2370 Size: 318 519 195 6 234 1098 Fragments arranged by size: 1098 519 318 234 195 6 NaeI GCC'GGC Cuts at: 0 92 711 2370 Size: 92 619 1659 Fragments arranged by size: 1659 619 92 NarI GG'CG_CC Cuts at: 0 444 713 2076 2370 Size: 444 269 1363 294 Fragments arranged by size: 1363 444 294 269 NcoI C'CATG_G Cuts at: 0 200 1661 1712 2370 Size: 200 1461 51 658 Fragments arranged by size: 1461 658 200 51 NdeI CA'TA_TG Cuts at: 0 1289 2370 Size: 1289 1081 NgoMIV G'CCGG_C Cuts at: 0 90 709 2370 Size: 90 619 1661 Fragments arranged by size: 1661 619 90 NsiI A_TGCA'T Cuts at: 0 2334 2370 Size: 2334 36 NspI r_CATG'y Cuts at: 0 812 960 1320 1686 1722 2016 2370 Size: 812 148 360 366 36 294 354 Fragments arranged by size: 812 366 360 354 294 148 36 PasI CC'CwG_GG Cuts at: 0 1668 2370 Size: 1668 702 PciI A'CATG_T Cuts at: 0 1316 2370 Size: 1316 1054 PflMI CCAn_nnn'nTGG Cuts at: 0 1713 2370 Size: 1713 657 PfoI T'CCnGG_A Cuts at: 0 131 1120 2302 2354 2370 Size: 131 989 1182 52 16 Fragments arranged by size: 1182 989 131 52 16 PshAI GACnn'nnGTC Cuts at: 0 1152 1369 2233 2370 Size: 1152 217 864 137 Fragments arranged by size: 1152 864 217 137 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1672 1704 2370 Size: 1672 32 666 Fragments arranged by size: 1672 666 32 PstI C_TGCA'G Cuts at: 0 428 1037 1448 1541 1858 2084 2370 Size: 428 609 411 93 317 226 286 Fragments arranged by size: 609 428 411 317 286 226 93 PvuII CAG'CTG Cuts at: 0 318 837 1038 2370 Size: 318 519 201 1332 Fragments arranged by size: 1332 519 318 201 SapI GCTCTTCn'nnn_ Cuts at: 0 323 2370 Size: 323 2047 SfcI C'TryA_G Cuts at: 0 424 1033 1444 1537 1854 2080 2370 Size: 424 609 411 93 317 226 290 Fragments arranged by size: 609 424 411 317 290 226 93 SfoI GGC'GCC Cuts at: 0 445 714 2077 2370 Size: 445 269 1363 293 Fragments arranged by size: 1363 445 293 269 SgrAI Cr'CCGG_yG Cuts at: 0 709 2370 Size: 709 1661 SmaI CCC'GGG Cuts at: 0 1390 2258 2370 Size: 1390 868 112 Fragments arranged by size: 1390 868 112 SphI G_CATG'C Cuts at: 0 812 2370 Size: 812 1558 StuI AGG'CCT Cuts at: 0 523 634 1913 2370 Size: 523 111 1279 457 Fragments arranged by size: 1279 523 457 111 StyI C'CwwG_G Cuts at: 0 14 200 323 548 734 1415 1452 1661 Size: 14 186 123 225 186 681 37 209 Cuts at: 1661 1712 1811 2054 2370 Size: 51 99 243 316 Fragments arranged by size: 681 316 243 225 209 186 186 123 99 51 37 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1906 2370 Size: 1906 464 TatI w'GTAC_w Cuts at: 0 620 1073 1616 1721 2370 Size: 620 453 543 105 649 Fragments arranged by size: 649 620 543 453 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 113 145 2370 Size: 113 32 2225 Fragments arranged by size: 2225 113 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 315 2097 2370 Size: 315 1782 273 Fragments arranged by size: 1782 315 273 XmaI C'CCGG_G Cuts at: 0 1388 2256 2370 Size: 1388 868 114 Fragments arranged by size: 1388 868 114 ZraI GAC'GTC Cuts at: 0 720 2370 Size: 720 1650 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI AlwNI ApaLI ApoI AvaI AvrII BamHI BanI BbeI BbsI BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PshAI PsrI PstI PvuII SapI SfcI SfoI SgrAI SmaI SphI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI AscI AseI AsiSI BaeI BanII BcgI BclI BglII BlpI BmtI BpuEI BsaAI BsaBI BsiEI BsmI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NheI NotI NruI PacI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI