Restriction map for
pFN21AB9280
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AccIII AclI AgeI
AlwI AocI Aor51HI ApaI AseI
AsnI Asp700I AspI AviII AvrII
BalI BamHI BglI BglII BlnI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI Bst1107I BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DsaI
Eco47III Eco81I EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1360 AAATTC [GA]^AATT[CT]
AflII 789 CTTAAG C^TTAAG
AhdI 481 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1216 GTGCAC G^TGCAC
ApaLI 1216 GTGCAC G^TGCAC
ApoI 1360 AAATTC [GA]^AATT[CT]
AscI 371 GGCGCGCC GG^CGCGCC
AspEI 481 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1576 GGGCTC G[GA]GC[CT]^C
BbeI 472 GGCGCC GGCGC^C
BbrPI 1213 CACGTG CAC^GTG
BbvCI 182 CCTCAGC CCTCAGC(-5/-2)
BcgI 533 CGATCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1233 GGATAC GTATCC(6/5)
BfrI 789 CTTAAG C^TTAAG
BsaAI 1213 CACGTG [CT]AC^GT[GA]
BsaI 454 GAGACC GGTCTC(1/5)
BsiEI 34 CGGCCG CG[GA][CT]^CG
BspHI 1226 TCATGA T^CATGA
BspMI 11 GCAGGT ACCTGC(4/8)
BssHII 372 GCGCGC G^CGCGC
BssSI 73 CACGAG CACGAG(-5/-1)
BstBI 382 TTCGAA TT^CGAA
BstXI 227 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 34 CGGCCG [CT]^GGCC[GA]
EagI 34 CGGCCG C^GGCCG
Eam1105I 481 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 34 CGGCCG C^GGCCG
Eco52I 34 CGGCCG C^GGCCG
EcoNI 1679 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1053 GATATC GAT^ATC
EcoT14I 1035 CCTTGG C^C[AT][AT]GG
HphI 1084 GGTGA GGTGA(8/7)
KasI 472 GGCGCC G^GCGCC
NarI 472 GGCGCC GG^CGCC
NotI 33 GCGGCCGC GC^GGCCGC
NspI 425 GCATGT [GA]CATG^[CT]
NspV 382 TTCGAA TT^CGAA
PflMI 72 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1213 CACGTG CAC^GTG
PmlI 1213 CACGTG CAC^GTG
RcaI 1226 TCATGA T^CATGA
SfoI 472 GGCGCC GGC^GCC
SfuI 382 TTCGAA TT^CGAA
SgrAI 367 CACCGGCG C[GA]^CCGG[CT]G
StyI 1035 CCTTGG C^C[AT][AT]GG
Van91I 72 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 853 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 1001 GGTACC G^GTACC
1092 GGTACC
AflIII 892 ACACGT A^C[GA][CT]GT
1212 ACACGT
Asp718I 1001 GGTACC G^GTACC
1092 GGTACC
AvaI 190 CCCGAG C^[CT]CG[GA]G
211 CCCGAG
BbsI 906 GAAGAC GAAGAC(2/6)
1501 GAAGAC
BclI 551 TGATCA T^GATCA
1008 TGATCA
BcnI 716 CCCGG CC^[GC]GG
1673 CCCGG
BpmI 269 CTGGAG CTGGAG(16/14)
612 CTGGAG
BpuAI 906 GAAGAC GAAGAC(2/6)
1501 GAAGAC
BsmFI 57 GTCCC GGGAC(10/14)
1671 GTCCC
BsoBI 190 CCCGAG C^[CT]CG[GA]G
211 CCCGAG
BsrFI 250 ACCGGC [GA]^CCGG[CT]
368 ACCGGC
BstAPI 93 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
151 GCAGCCGCTGC
Cfr10I 250 ACCGGC [GA]^CCGG[CT]
368 ACCGGC
DrdI 456 GACCGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1642 GACAAGCACGTC
EarI 1701 CTCTTC CTCTTC(1/4)
1512 GAAGAG
FbaI 551 TGATCA T^GATCA
1008 TGATCA
HaeII 237 GGCGCT [GA]GCGC^[CT]
472 GGCGCC
KpnI 1001 GGTACC GGTAC^C
1092 GGTACC
Ksp632I 1701 CTCTTC CTCTTC(1/4)
1512 GAAGAG
MslI 701 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1431 CACTTTGGTG
NciI 716 CCCGG CC^[GC]GG
1673 CCCGG
SmlI 48 CTTGAG C^T[CT][GA]AG
789 CTTAAG
Tsp45I 1085 GTGAC ^GT[GC]AC
1651 GTCAC
XcmI 749 CCACCGCTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1563 CCACGCCCTCCCTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BbvI
BfaI BlpI BmyI Bpu1102I BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I