(Circular) (Six-base) MAPSORT of: pFN21AB9280.seq Check: 1316 from: 1 to: 6442 pFN21AB9280 6442 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3006 3097 3763 3006 Size: 91 666 5685 Fragments arranged by size: 5685 666 91 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4505 5889 6157 1632 Size: 2873 1384 268 1917 Fragments arranged by size: 2873 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2794 820 Size: 197 1777 4468 Fragments arranged by size: 4468 1777 197 AflIII A'CryG_T Cuts at: 1203 2897 3217 5341 6318 1203 Size: 1694 320 2124 977 1327 Fragments arranged by size: 2124 1694 1327 977 320 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 2491 5171 2491 Size: 2680 3762 AleI CACnn'nnGTG Cuts at: 1166 3440 1166 Size: 2274 4168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6442 AlwNI CAG_nnn'CTG Cuts at: 2104 2162 2939 4185 5757 2104 Size: 58 777 1246 1572 2789 Fragments arranged by size: 2789 1572 1246 777 58 ApaLI G'TGCA_C Cuts at: 3221 4499 5655 3221 Size: 1278 1156 4008 Fragments arranged by size: 4008 1278 1156 ApoI r'AATT_y Cuts at: 1493 3365 3751 3977 4129 6238 1493 Size: 1872 386 226 152 2109 1697 Fragments arranged by size: 2109 1872 1697 386 226 152 AscI GG'CGCG_CC Cuts at: 2377 2377 Size: 6442 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 2195 2216 3767 1870 Size: 73 252 21 1551 4545 Fragments arranged by size: 4545 1551 252 73 21 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2321 2477 3006 3097 3763 Size: 325 217 627 534 156 529 91 666 Cuts at: 3763 5218 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 666 627 534 529 325 217 156 91 BanII G_rGCy'C Cuts at: 1391 3585 3761 1391 Size: 2194 176 4072 Fragments arranged by size: 4072 2194 176 BbeI G_GCGC'C Cuts at: 2481 2481 Size: 6442 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2918 3513 928 Size: 971 1019 595 3857 Fragments arranged by size: 3857 1019 971 595 BbvCI CC'TCA_GC Cuts at: 2188 2188 Size: 6442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2527 2561 4633 4667 1903 Size: 34 590 34 2072 34 3678 Fragments arranged by size: 3678 2072 590 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3232 4334 5544 3232 Size: 1102 1210 4130 Fragments arranged by size: 4130 1210 1102 BclI T'GATC_A Cuts at: 1548 2556 3013 1548 Size: 1008 457 4977 Fragments arranged by size: 4977 1008 457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5053 136 Size: 107 122 71 631 605 3381 1525 Fragments arranged by size: 3381 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 2045 2405 3669 3859 2045 Size: 360 1264 190 4628 Fragments arranged by size: 4628 1264 360 190 Bme1580I G_kGCm'C Cuts at: 1790 2307 3225 4503 5659 1790 Size: 517 918 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 918 517 BmgBI CAC'GTC Cuts at: 1318 3655 1318 Size: 2337 4105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4173 5131 442 Size: 416 53 83 399 24 2756 958 1753 Fragments arranged by size: 2756 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2638 5102 1239 Size: 1056 343 2464 2579 Fragments arranged by size: 2579 2464 1056 343 Bpu10I CC'TnA_GC Cuts at: 2188 2506 2188 Size: 318 6124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2074 4567 5432 5730 2074 Size: 2493 865 298 2786 Fragments arranged by size: 2786 2493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 5105 882 Size: 617 954 2652 2219 Fragments arranged by size: 2652 2219 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3220 4233 493 Size: 711 2016 1013 2702 Fragments arranged by size: 2702 2016 1013 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2478 2492 4249 Size: 53 83 186 936 498 447 14 1757 Cuts at: 4249 4631 5266 275 Size: 382 635 1451 Fragments arranged by size: 1757 1451 936 635 498 447 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 4875 5273 5547 5694 6105 4875 Size: 398 274 147 411 5212 Fragments arranged by size: 5212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 1178 Size: 30 165 30 810 30 5377 Fragments arranged by size: 5377 810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 2292 2845 1186 Size: 956 150 553 4783 Fragments arranged by size: 4783 956 553 150 BseYI C'CCAG_C Cuts at: 2365 5645 6141 2365 Size: 3280 496 2666 Fragments arranged by size: 3280 2666 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5869 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 3892 4653 5681 1540 Size: 275 186 41 1850 761 1028 2301 Fragments arranged by size: 2301 1850 1028 761 275 186 41 BsiHKAI G_wGCw'C Cuts at: 2223 2875 3225 4503 4588 5659 2223 Size: 652 350 1278 85 1071 3006 Fragments arranged by size: 3006 1278 1071 652 350 85 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2307 2875 3225 3585 3761 4503 Size: 399 433 84 568 350 360 176 742 Cuts at: 4503 4588 5659 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 433 399 360 350 176 85 84 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 3231 4331 6061 3231 Size: 1100 1730 3612 Fragments arranged by size: 3612 1730 1100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2007 3797 844 Size: 1163 1790 3489 Fragments arranged by size: 3489 1790 1163 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2301 3673 4329 1394 Size: 429 221 257 1372 656 3507 Fragments arranged by size: 3507 1372 656 429 257 221 BsrDI GCAATG_nn' Cuts at: 65 1248 4937 5111 65 Size: 1183 3689 174 1396 Fragments arranged by size: 3689 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2373 5086 5273 1700 Size: 110 445 118 2713 187 2869 Fragments arranged by size: 2869 2713 445 187 118 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 2377 1925 Size: 452 5990 BssSI C'ACGA_G Cuts at: 2078 4502 5514 2078 Size: 2424 1012 3006 Fragments arranged by size: 3006 2424 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 2162 794 Size: 1310 58 5074 Fragments arranged by size: 5074 1310 58 BstBI TT'CG_AA Cuts at: 2388 5246 2388 Size: 2858 3584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2239 Size: 6442 BstYI r'GATC_y Cuts at: 1972 3772 4139 4524 4541 5993 6079 6091 6437 Size: 1800 367 385 17 1452 86 12 346 Cuts at: 6437 1972 Size: 1977 Fragments arranged by size: 1977 1800 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5890 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3062 487 Size: 683 56 1836 3867 Fragments arranged by size: 3867 1836 683 56 BtsI GCAGTG_nn' Cuts at: 2323 3958 4752 4772 2323 Size: 1635 794 20 3993 Fragments arranged by size: 3993 1635 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2467 3653 5449 809 Size: 507 1151 1186 1796 1802 Fragments arranged by size: 1802 1796 1186 1151 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 3889 4778 Size: 54 1425 50 275 18 209 1850 889 Cuts at: 4778 6407 8 Size: 1629 43 Fragments arranged by size: 1850 1629 1425 889 275 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2039 3889 1537 Size: 275 227 1850 4090 Fragments arranged by size: 4090 1850 275 227 EarI CTCTTCn'nnn_ Cuts at: 1353 3512 3712 4372 1353 Size: 2159 200 660 3423 Fragments arranged by size: 3423 2159 660 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2277 5029 5403 5549 387 Size: 1064 138 688 2752 374 146 1280 Fragments arranged by size: 2752 1280 1064 688 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2638 4505 5102 5889 6157 1239 Size: 393 663 343 1867 597 787 268 1524 Fragments arranged by size: 1867 1524 787 663 597 393 343 268 EcoNI CCTnn'n_nnAGG Cuts at: 3688 3688 Size: 6442 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 EcoRV GAT'ATC Cuts at: 3060 3060 Size: 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 6442 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2246 2481 5589 1678 Size: 568 235 3108 2531 Fragments arranged by size: 3108 2531 568 235 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2840 2872 3226 Size: 32 135 32 905 32 331 32 354 Cuts at: 3226 3258 5083 5115 5157 5189 1373 Size: 32 1825 32 42 32 2626 Fragments arranged by size: 2626 1825 905 354 331 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3786 4032 669 Size: 988 281 1848 246 3079 Fragments arranged by size: 3079 1848 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KasI G'GCGC_C Cuts at: 2477 2477 Size: 6442 KpnI G_GTAC'C Cuts at: 3010 3101 3767 3010 Size: 91 666 5685 Fragments arranged by size: 5685 666 91 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 2256 5556 5740 1305 Size: 799 152 3300 184 2007 Fragments arranged by size: 3300 2007 799 184 152 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2710 3440 4400 4759 4918 518 Size: 648 1544 730 960 359 159 2042 Fragments arranged by size: 2042 1544 960 730 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2162 2177 2759 3247 3272 3858 4535 Size: 407 149 15 582 488 25 586 677 Cuts at: 4535 5683 5928 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 586 582 488 407 245 149 25 15 NarI GG'CG_CC Cuts at: 2478 2478 Size: 6442 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 2039 3889 2039 Size: 1850 4592 NspI r_CATG'y Cuts at: 1132 1229 2434 3800 5345 1132 Size: 97 1205 1366 1545 2229 Fragments arranged by size: 2229 1545 1366 1205 97 PasI CC'CwG_GG Cuts at: 1348 3578 1348 Size: 2230 4212 PciI A'CATG_T Cuts at: 5341 5341 Size: 6442 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 1108 Size: 975 5467 PfoI T'CCnGG_A Cuts at: 3689 3689 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PmlI CAC'GTG Cuts at: 3220 3220 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2342 2374 2342 Size: 32 6410 PstI C_TGCA'G Cuts at: 830 2018 2109 2158 3794 830 Size: 1188 91 49 1636 3478 Fragments arranged by size: 3478 1636 1188 91 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 PvuII CAG'CTG Cuts at: 2013 3247 3272 2013 Size: 1234 25 5183 Fragments arranged by size: 5183 1234 25 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2014 2105 2154 3177 3790 4925 5606 Size: 219 969 91 49 1023 613 1135 681 Cuts at: 5606 5797 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1023 969 681 613 219 191 91 49 SfoI GGC'GCC Cuts at: 2479 2479 Size: 6442 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 2373 1700 Size: 673 5769 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2794 4546 5447 5709 820 Size: 197 926 110 741 1752 901 262 1553 Fragments arranged by size: 1752 1553 926 901 741 262 197 110 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3800 1132 Size: 2668 3774 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 3040 3881 513 Size: 552 1975 841 3074 Fragments arranged by size: 3074 1975 841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 2540 3137 4469 4486 4639 4824 Size: 1255 897 63 597 1332 17 153 185 Cuts at: 4824 5269 325 Size: 445 1498 Fragments arranged by size: 1498 1332 1255 897 597 445 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2941 3280 4688 Size: 274 80 33 51 1443 964 339 1408 Cuts at: 4688 6400 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1408 964 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 6402 6434 2213 Size: 32 4157 32 2221 Fragments arranged by size: 4157 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 2858 3778 2858 Size: 920 5522 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 3575 2761 Size: 814 5628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 4571 4571 Size: 6442 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI