Restriction map for
pFN21AB9280
(Linear) (Six-base) MAPSORT of: KIBB9280.seq Check: 5772 from: 1 to: 1737
KIBB9280.orf
With 182 enzymes: SgfI *
September 22, 2011 10:48 ..
Acc65I G'GTAC_C
Cuts at: 0 1001 1092 1737
Size: 1001 91 645
Fragments arranged by size:
1001 645 91
AflII C'TTAA_G
Cuts at: 0 789 1737
Size: 789 948
AflIII A'CryG_T
Cuts at: 0 892 1212 1737
Size: 892 320 525
Fragments arranged by size:
892 525 320
AhdI GACnn_n'nnGTC
Cuts at: 0 486 1737
Size: 486 1251
AleI CACnn'nnGTG
Cuts at: 0 1435 1737
Size: 1435 302
AlwNI CAG_nnn'CTG
Cuts at: 0 99 157 934 1737
Size: 99 58 777 803
Fragments arranged by size:
803 777 99 58
ApaLI G'TGCA_C
Cuts at: 0 1216 1737
Size: 1216 521
ApoI r'AATT_y
Cuts at: 0 1360 1737
Size: 1360 377
AscI GG'CGCG_CC
Cuts at: 0 372 1737
Size: 372 1365
AvaI C'yCGr_G
Cuts at: 0 190 211 1737
Size: 190 21 1526
Fragments arranged by size:
1526 190 21
BanI G'GyrC_C
Cuts at: 0 316 472 1001 1092 1737
Size: 316 156 529 91 645
Fragments arranged by size:
645 529 316 156 91
BanII G_rGCy'C
Cuts at: 0 1580 1737
Size: 1580 157
BbeI G_GCGC'C
Cuts at: 0 476 1737
Size: 476 1261
BbsI GAAGACnn'nnnn_
Cuts at: 0 913 1508 1737
Size: 913 595 229
Fragments arranged by size:
913 595 229
BbvCI CC'TCA_GC
Cuts at: 0 183 1737
Size: 183 1554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 522 556 1737
Size: 522 34 1181
Fragments arranged by size:
1181 522 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1227 1737
Size: 1227 510
BclI T'GATC_A
Cuts at: 0 551 1008 1737
Size: 551 457 729
Fragments arranged by size:
729 551 457
BlpI GC'TnA_GC
Cuts at: 0 40 400 1664 1737
Size: 40 360 1264 73
Fragments arranged by size:
1264 360 73 40
Bme1580I G_kGCm'C
Cuts at: 0 302 1220 1737
Size: 302 918 517
Fragments arranged by size:
918 517 302
BmgBI CAC'GTC
Cuts at: 0 1650 1737
Size: 1650 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1737
Size: 99 1638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 633 1737
Size: 290 343 1104
Fragments arranged by size:
1104 343 290
Bpu10I CC'TnA_GC
Cuts at: 0 183 501 1737
Size: 183 318 1236
Fragments arranged by size:
1236 318 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1737
Size: 69 1668
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 1737
Size: 448 1289
BsaAI yAC'GTr
Cuts at: 0 1215 1737
Size: 1215 522
BsaHI Gr'CG_yC
Cuts at: 0 26 473 487 1737
Size: 26 447 14 1250
Fragments arranged by size:
1250 447 26 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 1737
Size: 208 30 1499
Fragments arranged by size:
1499 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 287 840 1737
Size: 137 150 553 897
Fragments arranged by size:
897 553 150 137
BseYI C'CCAG_C
Cuts at: 0 360 1737
Size: 360 1377
BsiEI CG_ry'CG
Cuts at: 0 37 1737
Size: 37 1700
BsiHKAI G_wGCw'C
Cuts at: 0 218 870 1220 1737
Size: 218 652 350 517
Fragments arranged by size:
652 517 350 218
Bsp1286I G_dGCh'C
Cuts at: 0 218 302 870 1220 1580 1737
Size: 218 84 568 350 360 157
Fragments arranged by size:
568 360 350 218 157 84
BspHI T'CATG_A
Cuts at: 0 1226 1737
Size: 1226 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2 1737
Size: 2 1735
BsrBI CCG'CTC
Cuts at: 0 39 296 1668 1737
Size: 39 257 1372 69
Fragments arranged by size:
1372 257 69 39
BsrFI r'CCGG_y
Cuts at: 0 250 368 1737
Size: 250 118 1369
Fragments arranged by size:
1369 250 118
BssHII G'CGCG_C
Cuts at: 0 372 1737
Size: 372 1365
BssSI C'ACGA_G
Cuts at: 0 73 1737
Size: 73 1664
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 157 1737
Size: 99 58 1580
Fragments arranged by size:
1580 99 58
BstBI TT'CG_AA
Cuts at: 0 383 1737
Size: 383 1354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 1737
Size: 234 1503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1057 1737
Size: 1057 680
BtsI GCAGTG_nn'
Cuts at: 0 318 1737
Size: 318 1419
DrdI GACnn_nn'nnGTC
Cuts at: 0 462 1648 1737
Size: 462 1186 89
Fragments arranged by size:
1186 462 89
EaeI y'GGCC_r
Cuts at: 0 34 1737
Size: 34 1703
EagI C'GGCC_G
Cuts at: 0 34 1737
Size: 34 1703
EarI CTCTTCn'nnn_
Cuts at: 0 1507 1707 1737
Size: 1507 200 30
Fragments arranged by size:
1507 200 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 272 1737
Size: 272 1465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 633 1737
Size: 290 343 1104
Fragments arranged by size:
1104 343 290
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1683 1737
Size: 1683 54
EcoRV GAT'ATC
Cuts at: 0 1055 1737
Size: 1055 682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1737
Size: 900 837
HaeII r_GCGC'y
Cuts at: 0 241 476 1737
Size: 241 235 1261
Fragments arranged by size:
1261 241 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 835 867 1221 1253 1737
Size: 472 32 331 32 354 32 484
Fragments arranged by size:
484 472 354 331 32 32 32
KasI G'GCGC_C
Cuts at: 0 472 1737
Size: 472 1265
KpnI G_GTAC'C
Cuts at: 0 1005 1096 1737
Size: 1005 91 641
Fragments arranged by size:
1005 641 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 251 1737
Size: 99 152 1486
Fragments arranged by size:
1486 152 99
MslI CAynn'nnrTG
Cuts at: 0 705 1435 1737
Size: 705 730 302
Fragments arranged by size:
730 705 302
MspA1I CmG'CkG
Cuts at: 0 8 157 172 754 1242 1267 1737
Size: 8 149 15 582 488 25 470
Fragments arranged by size:
582 488 470 149 25 15 8
NarI GG'CG_CC
Cuts at: 0 473 1737
Size: 473 1264
NotI GC'GGCC_GC
Cuts at: 0 34 1737
Size: 34 1703
NspI r_CATG'y
Cuts at: 0 429 1737
Size: 429 1308
PasI CC'CwG_GG
Cuts at: 0 1573 1737
Size: 1573 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 1737
Size: 78 1659
PfoI T'CCnGG_A
Cuts at: 0 1684 1737
Size: 1684 53
PmlI CAC'GTG
Cuts at: 0 1215 1737
Size: 1215 522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 337 369 1737
Size: 337 32 1368
Fragments arranged by size:
1368 337 32
PstI C_TGCA'G
Cuts at: 0 13 104 153 1737
Size: 13 91 49 1584
Fragments arranged by size:
1584 91 49 13
PvuII CAG'CTG
Cuts at: 0 8 1242 1267 1737
Size: 8 1234 25 470
Fragments arranged by size:
1234 470 25 8
SfcI C'TryA_G
Cuts at: 0 9 100 149 1172 1737
Size: 9 91 49 1023 565
Fragments arranged by size:
1023 565 91 49 9
SfoI GGC'GCC
Cuts at: 0 474 1737
Size: 474 1263
SgrAI Cr'CCGG_yG
Cuts at: 0 368 1737
Size: 368 1369
SmlI C'TyrA_G
Cuts at: 0 48 789 1737
Size: 48 741 948
Fragments arranged by size:
948 741 48
StyI C'CwwG_G
Cuts at: 0 1035 1737
Size: 1035 702
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 535 1132 1737
Size: 472 63 597 605
Fragments arranged by size:
605 597 472 63
TatI w'GTAC_w
Cuts at: 0 936 1275 1737
Size: 936 339 462
Fragments arranged by size:
936 462 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 1737
Size: 208 32 1497
Fragments arranged by size:
1497 208 32
XbaI T'CTAG_A
Cuts at: 0 853 1737
Size: 853 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 1570 1737
Size: 756 814 167
Fragments arranged by size:
814 756 167
Enzymes that do cut:
Acc65I AflII AflIII AhdI AleI AlwNI ApaLI ApoI
AscI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstXI BtgZI BtsI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRV FalI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NarI NotI NspI
PasI PflMI PfoI PmlI PsrI PstI PvuII SfcI
SfoI SgrAI SmlI StyI TaqII TatI TstI XbaI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AgeI AlfI
AloI ApaI AseI AsiSI AvrII BaeI BamHI BfrBI
BglI BglII BmrI BmtI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BstEII BstYI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII EcoICRI EcoO109I
EcoRI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI XmnI ZraI