(Circular) (Six-base) MAPSORT of: pFN21AE2373.seq Check: 9077 from: 1 to: 8134 pFN21AE2373 8134 bp With 182 enzymes: SgfI * September 6, 2011 15:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3955 4538 5279 3955 Size: 583 741 6810 Fragments arranged by size: 6810 741 583 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5944 278 Size: 53 83 186 936 4408 2468 Fragments arranged by size: 4408 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5477 1937 Size: 3540 4594 Acc65I G'GTAC_C Cuts at: 5455 5455 Size: 8134 AclI AA'CG_TT Cuts at: 1563 6261 6634 1563 Size: 4698 373 3063 Fragments arranged by size: 4698 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 2599 4424 6197 7581 7849 1632 Size: 917 50 1825 1773 1384 268 1917 Fragments arranged by size: 1917 1825 1773 1384 917 268 50 AfeI AGC'GCT Cuts at: 2280 3224 2280 Size: 944 7190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7937 AflIII A'CryG_T Cuts at: 1203 2108 2738 5031 7033 8010 1203 Size: 905 630 2293 2002 977 1327 Fragments arranged by size: 2293 2002 1327 977 905 630 AgeI A'CCGG_T Cuts at: 6965 6965 Size: 8134 AhdI GACnn_n'nnGTC Cuts at: 6863 6863 Size: 8134 AleI CACnn'nnGTG Cuts at: 1166 2585 3023 5405 1166 Size: 1419 438 2382 3895 Fragments arranged by size: 3895 2382 1419 438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2393 4235 4374 1184 Size: 1209 1842 139 4944 Fragments arranged by size: 4944 1842 1209 139 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4304 4336 4304 Size: 32 8102 AlwNI CAG_nnn'CTG Cuts at: 2365 2527 2599 2896 4378 4573 4587 4900 5135 Size: 162 72 297 1482 195 14 313 235 Cuts at: 5135 5187 5306 5877 7449 2365 Size: 52 119 571 1572 3050 Fragments arranged by size: 3050 1572 1482 571 313 297 235 195 162 119 72 52 14 ApaI G_GGCC'C Cuts at: 3494 3703 4105 4165 5298 3494 Size: 209 402 60 1133 6330 Fragments arranged by size: 6330 1133 402 209 60 ApaLI G'TGCA_C Cuts at: 2629 6191 7347 2629 Size: 3562 1156 3416 Fragments arranged by size: 3562 3416 1156 ApoI r'AATT_y Cuts at: 1493 3561 5443 5669 5821 7930 1493 Size: 2068 1882 226 152 2109 1697 Fragments arranged by size: 2109 2068 1882 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6688 160 Size: 872 5656 1606 Fragments arranged by size: 5656 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 AvaI C'yCGr_G Cuts at: 1870 1943 4550 5459 1870 Size: 73 2607 909 4545 Fragments arranged by size: 4545 2607 909 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4911 4944 4911 Size: 33 8101 BamHI G'GATC_C Cuts at: 3357 5464 5831 3357 Size: 2107 367 5660 Fragments arranged by size: 5660 2107 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2587 2995 3289 4002 4056 Size: 325 217 627 800 408 294 713 54 Cuts at: 4056 4511 5455 6910 618 Size: 455 944 1455 1842 Fragments arranged by size: 1842 1455 944 800 713 627 455 408 325 294 217 54 BanII G_rGCy'C Cuts at: 1391 2059 2357 2413 3494 3648 3703 3794 4105 Size: 668 298 56 1081 154 55 91 311 Cuts at: 4105 4165 5298 5453 1391 Size: 60 1133 155 4072 Fragments arranged by size: 4072 1133 1081 668 311 298 155 154 91 60 56 55 BbeI G_GCGC'C Cuts at: 4060 4515 4060 Size: 455 7679 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3145 3940 4528 5342 928 Size: 971 1246 795 588 814 3720 Fragments arranged by size: 3720 1246 971 814 795 588 BbvCI CC'TCA_GC Cuts at: 3158 4640 5307 3158 Size: 1482 667 5985 Fragments arranged by size: 5985 1482 667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6325 6359 1903 Size: 34 50 34 4304 34 3678 Fragments arranged by size: 4304 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4912 6026 7236 4912 Size: 1114 1210 5810 Fragments arranged by size: 5810 1210 1114 BclI T'GATC_A Cuts at: 1548 1548 Size: 8134 BfrBI ATG'CAT Cuts at: 3312 3312 Size: 8134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2908 3004 3480 Size: 107 122 71 631 605 1236 96 476 Cuts at: 3480 3489 4014 5085 6745 136 Size: 9 525 1071 1660 1525 Fragments arranged by size: 1660 1525 1236 1071 631 605 525 476 122 107 96 71 9 BglII A'GATC_T Cuts at: 8129 8129 Size: 8134 BlpI GC'TnA_GC Cuts at: 4366 5551 4366 Size: 1185 6949 Bme1580I G_kGCm'C Cuts at: 1790 2592 2633 3494 3703 4105 4165 4849 4864 Size: 802 41 861 209 402 60 684 15 Cuts at: 4864 5298 6195 7351 1790 Size: 434 897 1156 2573 Fragments arranged by size: 2573 1156 897 861 802 684 434 402 209 60 41 15 BmgBI CAC'GTC Cuts at: 1318 3057 1318 Size: 1739 6395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2375 2494 2587 Size: 416 53 83 399 24 958 119 93 Cuts at: 2587 2812 5865 6823 442 Size: 225 3053 958 1753 Fragments arranged by size: 3053 1753 958 958 416 399 225 119 93 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3746 4796 1056 Size: 2690 1050 4394 Fragments arranged by size: 4394 2690 1050 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2215 2873 2947 3122 3209 3318 3929 4234 Size: 976 658 74 175 87 109 611 305 Cuts at: 4234 4595 4679 4691 4802 4922 5267 5315 6794 Size: 361 84 12 111 120 345 48 1479 Cuts at: 6794 1239 Size: 2579 Fragments arranged by size: 2579 1479 976 658 611 361 345 305 175 120 111 109 87 84 74 48 12 Bpu10I CC'TnA_GC Cuts at: 3158 4379 4640 4952 5307 3158 Size: 1221 261 312 355 5985 Fragments arranged by size: 5985 1221 355 312 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 2903 6259 7124 7422 2207 Size: 696 3356 865 298 2919 Fragments arranged by size: 3356 2919 865 696 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2037 3329 4655 6797 882 Size: 617 538 1292 1326 2142 2219 Fragments arranged by size: 2219 2142 1326 1292 617 538 BsaAI yAC'GTr Cuts at: 493 1204 5431 5925 493 Size: 711 4227 494 2702 Fragments arranged by size: 4227 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3782 8128 3782 Size: 4346 3788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2455 2883 4057 4452 Size: 53 83 186 936 922 428 1174 395 Cuts at: 4452 4512 5941 6323 6958 275 Size: 60 1429 382 635 1451 Fragments arranged by size: 1451 1429 1174 936 922 635 428 395 382 186 83 60 53 BsaWI w'CCGG_w Cuts at: 3303 3377 5418 6567 6965 7239 7386 7797 3303 Size: 74 2041 1149 398 274 147 411 3640 Fragments arranged by size: 3640 2041 1149 411 398 274 147 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4232 4262 4620 4650 1178 Size: 30 165 30 2829 30 358 30 4662 Fragments arranged by size: 4662 2829 358 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 2255 3137 3146 4037 4205 4247 4628 Size: 815 254 882 9 891 168 42 381 Cuts at: 4628 4919 4922 4925 4928 1186 Size: 291 3 3 3 4392 Fragments arranged by size: 4392 891 882 815 381 291 254 168 42 9 3 3 3 BseYI C'CCAG_C Cuts at: 2060 2275 2634 2807 3219 3225 3747 4097 4273 Size: 215 359 173 412 6 522 350 176 Cuts at: 4273 4414 4851 4890 5056 7337 7833 2060 Size: 141 437 39 166 2281 496 2361 Fragments arranged by size: 2361 2281 522 496 437 412 359 350 215 176 173 166 141 39 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3227 3983 4151 4505 4544 1163 Size: 573 1491 756 168 354 39 4753 Fragments arranged by size: 4753 1491 756 573 354 168 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3905 5584 6345 7373 1540 Size: 275 186 1904 1679 761 1028 2301 Fragments arranged by size: 2301 1904 1679 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 2633 2738 3822 6195 6280 7351 2538 Size: 95 105 1084 2373 85 1071 3321 Fragments arranged by size: 3321 2373 1084 1071 105 95 85 BsmI GAATG_Cn' Cuts at: 5645 5738 5645 Size: 93 8041 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 4596 2099 Size: 2497 5637 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2357 2413 2538 2592 2633 2738 Size: 399 269 298 56 125 54 41 105 Cuts at: 2738 3494 3648 3703 3794 3822 4105 4165 4849 Size: 756 154 55 91 28 283 60 684 Cuts at: 4849 4864 5298 5453 6195 6280 7351 1391 Size: 15 434 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 756 742 684 434 399 298 283 269 155 154 125 105 91 85 60 56 55 54 41 28 15 BspEI T'CCGG_A Cuts at: 3303 3377 7797 3303 Size: 74 4420 3640 Fragments arranged by size: 4420 3640 74 BspHI T'CATG_A Cuts at: 6023 7753 6023 Size: 1730 6404 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 3955 4105 4538 5216 5279 5489 844 Size: 1763 1348 150 433 678 63 210 3489 Fragments arranged by size: 3489 1763 1348 678 433 210 150 63 BsrBI CCG'CTC Cuts at: 1394 1823 2479 2854 3136 6021 1394 Size: 429 656 375 282 2885 3507 Fragments arranged by size: 3507 2885 656 429 375 282 BsrDI GCAATG_nn' Cuts at: 65 1248 2983 3327 6629 6803 65 Size: 1183 1735 344 3302 174 1396 Fragments arranged by size: 3302 1735 1396 1183 344 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2555 2564 4728 4869 6778 6965 1700 Size: 110 745 9 2164 141 1909 187 2869 Fragments arranged by size: 2869 2164 1909 745 187 141 110 9 BsrGI T'GTAC_A Cuts at: 96 5178 96 Size: 5082 3052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8134 BssSI C'ACGA_G Cuts at: 4187 6194 7206 4187 Size: 2007 1012 5115 Fragments arranged by size: 5115 2007 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3427 4002 794 Size: 2633 575 4926 Fragments arranged by size: 4926 2633 575 BstBI TT'CG_AA Cuts at: 6938 6938 Size: 8134 BstEII G'GTnAC_C Cuts at: 1184 3140 3923 1184 Size: 1956 783 5395 Fragments arranged by size: 5395 1956 783 BstXI CCAn_nnnn'nTGG Cuts at: 2674 4942 5075 2674 Size: 2268 133 5733 Fragments arranged by size: 5733 2268 133 BstYI r'GATC_y Cuts at: 1972 2721 3357 5464 5831 6216 6233 7685 7771 Size: 749 636 2107 367 385 17 1452 86 Cuts at: 7771 7783 8129 1972 Size: 12 346 1977 Fragments arranged by size: 2107 1977 1452 749 636 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3092 3129 3092 Size: 37 8097 BtgI C'CryG_G Cuts at: 513 1065 2382 3080 4084 513 Size: 552 1317 698 1004 4563 Fragments arranged by size: 4563 1317 1004 698 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2479 3811 5239 487 Size: 683 56 1253 1332 1428 3382 Fragments arranged by size: 3382 1428 1332 1253 683 56 BtsI GCAGTG_nn' Cuts at: 3239 5362 5650 6444 6464 3239 Size: 2123 288 794 20 4909 Fragments arranged by size: 4909 2123 794 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2740 2775 3310 3345 529 Size: 35 2176 35 535 35 5318 Fragments arranged by size: 5318 2176 535 35 35 35 DraI TTT'AAA Cuts at: 5438 5438 Size: 8134 DraIII CAC_nnn'GTG Cuts at: 2542 2542 Size: 8134 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2921 3149 7141 809 Size: 507 1605 228 3992 1802 Fragments arranged by size: 3992 1802 1605 507 228 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2434 2460 Size: 54 1425 50 275 18 453 151 26 Cuts at: 2460 2553 2664 2748 2937 3261 3902 4044 4440 Size: 93 111 84 189 324 641 142 396 Cuts at: 4440 4767 4944 5581 6470 8099 8 Size: 327 177 637 889 1629 43 Fragments arranged by size: 1629 1425 889 641 637 453 396 327 324 275 189 177 151 142 111 93 84 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3902 5581 1537 Size: 275 2090 1679 4090 Fragments arranged by size: 4090 2090 1679 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2136 2963 3596 3944 6064 1353 Size: 711 72 827 633 348 2120 3423 Fragments arranged by size: 3423 2120 827 711 633 348 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2393 2659 3059 3185 3497 6721 Size: 1064 138 804 266 400 126 312 3224 Cuts at: 6721 7095 7241 387 Size: 374 146 1280 Fragments arranged by size: 3224 1280 1064 804 400 374 312 266 146 138 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2549 2599 2873 2947 3122 3209 Size: 393 583 334 50 274 74 175 87 Cuts at: 3209 3318 3929 4234 4424 4595 4679 4691 4802 Size: 109 611 305 190 171 84 12 111 Cuts at: 4802 4922 5267 5315 6197 6794 7581 7849 1239 Size: 120 345 48 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 611 597 583 393 345 334 305 274 268 190 175 171 120 111 109 87 84 74 50 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 4955 4955 Size: 8134 EcoO109I rG'GnC_Cy Cuts at: 2827 3491 3619 3699 3700 3718 3735 4101 4162 Size: 664 128 80 1 18 17 366 61 Cuts at: 4162 4375 4423 5042 5281 5295 5355 2827 Size: 213 48 619 239 14 60 5606 Fragments arranged by size: 5606 664 619 366 239 213 128 80 61 60 48 18 17 14 1 EcoRI G'AATT_C Cuts at: 5443 5821 5443 Size: 378 7756 FspI TGC'GCA Cuts at: 6640 6640 Size: 8134 HaeII r_GCGC'y Cuts at: 1678 2282 2896 3183 3226 3252 3870 4060 4150 Size: 604 614 287 43 26 618 190 90 Cuts at: 4150 4159 4515 7281 1678 Size: 9 356 2766 2531 Fragments arranged by size: 2766 2531 618 614 604 356 287 190 90 43 26 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2677 2709 3388 3420 4232 Size: 32 135 32 1105 32 679 32 812 Cuts at: 4232 4264 6775 6807 6849 6881 1373 Size: 32 2511 32 42 32 2626 Fragments arranged by size: 2626 2511 1105 812 679 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5478 5724 669 Size: 988 281 3540 246 3079 Fragments arranged by size: 3540 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4325 748 Size: 3577 4557 HpaI GTT'AAC Cuts at: 5724 5724 Size: 8134 KasI G'GCGC_C Cuts at: 4056 4511 4056 Size: 455 7679 KpnI G_GTAC'C Cuts at: 5459 5459 Size: 8134 MfeI C'AATT_G Cuts at: 5733 5733 Size: 8134 MluI A'CGCG_T Cuts at: 2108 8010 2108 Size: 5902 2232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4056 4840 7248 7432 1305 Size: 2751 784 2408 184 2007 Fragments arranged by size: 2751 2408 2007 784 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2436 2462 2666 4046 4946 Size: 54 1425 343 604 26 204 1380 900 Cuts at: 4946 10 Size: 3198 Fragments arranged by size: 3198 1425 1380 900 604 343 204 54 26 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2930 3023 3611 5036 5405 6092 Size: 648 1419 345 93 588 1425 369 687 Cuts at: 6092 6451 6610 518 Size: 359 159 2042 Fragments arranged by size: 2042 1425 1419 687 648 588 369 359 345 159 93 MspA1I CmG'CkG Cuts at: 1606 2384 2800 3082 3162 3466 3751 3907 4174 Size: 778 416 282 80 304 285 156 267 Cuts at: 4174 4348 4358 4393 4438 4851 4876 4900 5550 Size: 174 10 35 45 413 25 24 650 Cuts at: 5550 6227 7375 7620 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 778 677 650 416 413 304 285 282 267 245 174 156 80 45 35 25 24 10 NarI GG'CG_CC Cuts at: 4057 4512 4057 Size: 455 7679 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7582 NdeI CA'TA_TG Cuts at: 387 387 Size: 8134 NheI G'CTAG_C Cuts at: 1052 3742 4792 1052 Size: 2690 1050 4394 Fragments arranged by size: 4394 2690 1050 NotI GC'GGCC_GC Cuts at: 3902 5581 3902 Size: 1679 6455 NsiI A_TGCA'T Cuts at: 3314 3314 Size: 8134 NspI r_CATG'y Cuts at: 1132 1229 5035 5231 5492 7037 1132 Size: 97 3806 196 261 1545 2229 Fragments arranged by size: 3806 2229 1545 261 196 97 PasI CC'CwG_GG Cuts at: 1348 4283 5300 1348 Size: 2935 1017 4182 Fragments arranged by size: 4182 2935 1017 PciI A'CATG_T Cuts at: 5031 7033 5031 Size: 2002 6132 PflMI CCAn_nnn'nTGG Cuts at: 1108 2545 5291 1108 Size: 1437 2746 3951 Fragments arranged by size: 3951 2746 1437 PfoI T'CCnGG_A Cuts at: 2231 3555 3687 4308 2231 Size: 1324 132 621 6057 Fragments arranged by size: 6057 1324 621 132 PmeI GTTT'AAAC Cuts at: 5438 5438 Size: 8134 PmlI CAC'GTG Cuts at: 5431 5431 Size: 8134 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2725 2757 6203 6235 7716 7748 2725 Size: 32 3446 32 1481 32 3111 Fragments arranged by size: 3446 3111 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 3619 3735 4375 5042 2827 Size: 792 116 640 667 5919 Fragments arranged by size: 5919 792 667 640 116 PshAI GACnn'nnGTC Cuts at: 1613 3341 5342 1613 Size: 1728 2001 4405 Fragments arranged by size: 4405 2001 1728 PsiI TTA'TAA Cuts at: 5704 5704 Size: 8134 PspOMI G'GGCC_C Cuts at: 3490 3699 4101 4161 5294 3490 Size: 209 402 60 1133 6330 Fragments arranged by size: 6330 1133 402 209 60 PstI C_TGCA'G Cuts at: 830 2208 2472 2523 3432 3597 4230 4353 4791 Size: 1378 264 51 909 165 633 123 438 Cuts at: 4791 5352 5486 830 Size: 561 134 3478 Fragments arranged by size: 3478 1378 909 633 561 438 264 165 134 123 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8134 PvuII CAG'CTG Cuts at: 3751 4174 4348 4393 4438 4876 4900 3751 Size: 423 174 45 45 438 24 6985 Fragments arranged by size: 6985 438 423 174 45 45 24 SacII CC_GC'GG Cuts at: 2385 3083 2385 Size: 698 7436 SalI G'TCGA_C Cuts at: 1936 5476 1936 Size: 3540 4594 SanDI GG'GwC_CC Cuts at: 3619 3735 5042 3619 Size: 116 1307 6711 Fragments arranged by size: 6711 1307 116 SapI GCTCTTCn'nnn_ Cuts at: 2963 3596 3944 2963 Size: 633 348 7153 Fragments arranged by size: 7153 633 348 SbfI CC_TGCA'GG Cuts at: 5486 5486 Size: 8134 ScaI AGT'ACT Cuts at: 6382 8094 6382 Size: 1712 6422 SexAI A'CCwGG_T Cuts at: 2538 4677 2538 Size: 2139 5995 SfcI C'TryA_G Cuts at: 826 1045 2204 2468 2519 3428 3593 4129 4226 Size: 219 1159 264 51 909 165 536 97 Cuts at: 4226 4349 4787 5348 5482 6617 7298 7489 826 Size: 123 438 561 134 1135 681 191 1471 Fragments arranged by size: 1471 1159 1135 909 681 561 536 438 264 219 191 165 134 123 97 51 SfiI GGCCn_nnn'nGGCC Cuts at: 3480 3489 3480 Size: 9 8125 SfoI GGC'GCC Cuts at: 4058 4513 4058 Size: 455 7679 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8134 SmaI CCC'GGG Cuts at: 1872 5461 1872 Size: 3589 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2918 6238 7139 7401 820 Size: 197 926 279 696 3320 901 262 1553 Fragments arranged by size: 3320 1553 926 901 696 279 262 197 SnaBI TAC'GTA Cuts at: 493 5925 493 Size: 5432 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8134 SphI G_CATG'C Cuts at: 1132 5231 5492 1132 Size: 4099 261 3774 Fragments arranged by size: 4099 3774 261 SspI AAT'ATT Cuts at: 5 52 6058 5 Size: 47 6006 2081 Fragments arranged by size: 6006 2081 47 StuI AGG'CCT Cuts at: 1517 2075 5372 1517 Size: 558 3297 4279 Fragments arranged by size: 4279 3297 558 StyI C'CwwG_G Cuts at: 513 1065 2076 4935 5573 513 Size: 552 1011 2859 638 3074 Fragments arranged by size: 3074 2859 1011 638 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2648 3217 3233 3742 3949 5413 6161 Size: 1255 1068 569 16 509 207 1464 748 Cuts at: 6161 6178 6331 6516 6961 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1464 1255 1068 748 569 509 445 207 185 153 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5178 6380 8092 Size: 274 80 33 51 1443 3201 1202 1712 Cuts at: 8092 96 Size: 138 Fragments arranged by size: 3201 1712 1443 1202 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3828 3860 8094 8126 3828 Size: 32 4234 32 3836 Fragments arranged by size: 4234 3836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3055 1316 Size: 1739 6395 XbaI T'CTAG_A Cuts at: 5470 5470 Size: 8134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3802 4297 4819 3802 Size: 495 522 7117 Fragments arranged by size: 7117 522 495 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8134 XmaI C'CCGG_G Cuts at: 1870 5459 1870 Size: 3589 4545 XmnI GAAnn'nnTTC Cuts at: 5384 6263 5384 Size: 879 7255 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5942 276 Size: 53 83 186 936 4408 2468 Fragments arranged by size: 4408 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BplI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacI SrfI SwaI