Restriction map for
pFN21AE3330
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AclI AflII AflIII
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AvrII BbeI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaI BsmBI BsmI
BspDI BspLU11I Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eco47III Eco81I EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 442 GGCGTC G[GA]^CG[CT]C
AgeI 1445 ACCGGT A^CCGGT
AhdI 2020 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2187 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2020 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 902 TGCGCA TGC^GCA
BamHI 114 GGATCC G^GATCC
BbrPI 1828 CACGTG CAC^GTG
BfaI 1864 CTAG C^TAG
BglII 1889 AGATCT A^GATCT
BsaBI 324 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 442 GGCGTC G[GA]^CG[CT]C
BsiWI 1149 CGTACG C^GTACG
Bsp1407I 1214 TGTACA T^GTACA
BspHI 2171 TCATGA T^CATGA
BsrGI 1214 TGTACA T^GTACA
BssHII 721 GCGCGC G^CGCGC
Eam1105I 2020 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1906 CTCTTC CTCTTC(1/4)
EcoRI 1687 GAATTC G^AATTC
FspI 902 TGCGCA TGC^GCA
Hin1I 442 GGCGTC G[GA]^CG[CT]C
HincII 2461 GTCAAC GT[CT]^[GA]AC
HindII 2461 GTCAAC GT[CT]^[GA]AC
Ksp632I 1906 CTCTTC CTCTTC(1/4)
MaeI 1864 CTAG C^TAG
MamI 324 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1370 TTAA T^TAA
PinAI 1445 ACCGGT A^CCGGT
PmaCI 1828 CACGTG CAC^GTG
PmlI 1828 CACGTG CAC^GTG
RcaI 2171 TCATGA T^CATGA
SphI 899 GCATGC GCATG^C
SspBI 1214 TGTACA T^GTACA
Tru9I 1370 TTAA T^TAA
XmnI 2187 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 645 GTCTAC GT^[AC][GT]AC
1459 GTCTAC
AccIII 332 TCCGGA T^CCGGA
2114 TCCGGA
AcsI 186 GAATTT [GA]^AATT[CT]
1687 GAATTC
Alw44I 545 GTGCAC G^TGCAC
1777 GTGCAC
ApaLI 545 GTGCAC G^TGCAC
1777 GTGCAC
ApoI 186 GAATTT [GA]^AATT[CT]
1687 GAATTC
BsaAI 1828 CACGTG [CT]AC^GT[GA]
2356 TACGTG
BseAI 332 TCCGGA T^CCGGA
2114 TCCGGA
BsmAI 1190 GAGAC GTCTC(1/5)
2764 GAGAC
BspEI 332 TCCGGA T^CCGGA
2114 TCCGGA
BsrBI 813 CCGCTC CCGCTC(-3/-3)
823 CCGCTC
BsrDI 1856 GCAATG GCAATG(2/0)
2336 GCAATG
BssSI 505 CACGAG CACGAG(-5/-1)
1176 CTCGTG
BstAPI 2124 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2544 GCAGCAGGTGC
BstEII 1468 GGTCACC G^GT[ACGT]ACC
1626 GGTGACC
BstPI 1468 GGTCACC G^GT[ACGT]ACC
1626 GGTGACC
BstXI 504 CCACGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
569 CCATTTATGTGG
EcoNI 1548 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2043 CCTGTGGGAGG
EcoO65I 1468 GGTCACC G^GT[ACGT]ACC
1626 GGTGACC
KspI 22 CCGCGG CCGC^GG
316 CCGCGG
MroI 332 TCCGGA T^CCGGA
2114 TCCGGA
NcoI 2429 CCATGG C^CATGG
2645 CCATGG
NotI 27 GCGGCCGC GC^GGCCGC
58 GCGGCCGC
NspI 899 GCATGC [GA]CATG^[CT]
1277 GCATGT
PleI 580 GACTC GAGTC(4/5)
2014 GACTC
SacII 22 CCGCGG CCGC^GG
316 CCGCGG
SfiI 1977 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2484 GGCCTATGGGGCC
TaqI 2012 TCGA T^CGA
2177 TCGA
TthHB8I 2012 TCGA T^CGA
2177 TCGA
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmaCI XmaI