Restriction map for
pFN21AE3330
(Linear) (Six-base) MAPSORT of: KIEE3330.seq Check: 8928 from: 1 to: 2829
KIEE3330.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2115 2538 2787 2829
Size: 2115 423 249 42
Fragments arranged by size:
2115 423 249 42
AccI GT'mk_AC
Cuts at: 0 646 1460 2829
Size: 646 814 1369
Fragments arranged by size:
1369 814 646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 571 826 1546 1641 2040 2829
Size: 77 494 255 720 95 399 789
Fragments arranged by size:
789 720 494 399 255 95 77
AgeI A'CCGG_T
Cuts at: 0 1445 2829
Size: 1445 1384
AhdI GACnn_n'nnGTC
Cuts at: 0 2025 2829
Size: 2025 804
AleI CACnn'nnGTG
Cuts at: 0 2588 2829
Size: 2588 241
AlwNI CAG_nnn'CTG
Cuts at: 0 372 388 1644 1664 2130 2767 2829
Size: 372 16 1256 20 466 637 62
Fragments arranged by size:
1256 637 466 372 62 20 16
ApaI G_GGCC'C
Cuts at: 0 1023 1123 1547 2366 2715 2829
Size: 1023 100 424 819 349 114
Fragments arranged by size:
1023 819 424 349 114 100
ApaLI G'TGCA_C
Cuts at: 0 545 1777 2829
Size: 545 1232 1052
Fragments arranged by size:
1232 1052 545
ApoI r'AATT_y
Cuts at: 0 186 1687 2829
Size: 186 1501 1142
Fragments arranged by size:
1501 1142 186
AvaI C'yCGr_G
Cuts at: 0 5 728 1187 1488 2283 2829
Size: 5 723 459 301 795 546
Fragments arranged by size:
795 723 546 459 301 5
BamHI G'GATC_C
Cuts at: 0 114 2829
Size: 114 2715
BanI G'GyrC_C
Cuts at: 0 732 2433 2478 2550 2829
Size: 732 1701 45 72 279
Fragments arranged by size:
1701 732 279 72 45
BanII G_rGCy'C
Cuts at: 0 13 883 1023 1123 1547 1766 2366 2715
Size: 13 870 140 100 424 219 600 349
Cuts at: 2715 2829
Size: 114
Fragments arranged by size:
870 600 424 349 219 140 114 100
13
BbsI GAAGACnn'nnnn_
Cuts at: 0 343 430 2046 2829
Size: 343 87 1616 783
Fragments arranged by size:
1616 783 343 87
BglI GCCn_nnn'nGGC
Cuts at: 0 55 1619 1984 2491 2829
Size: 55 1564 365 507 338
Fragments arranged by size:
1564 507 365 338 55
BglII A'GATC_T
Cuts at: 0 1889 2829
Size: 1889 940
Bme1580I G_kGCm'C
Cuts at: 0 549 735 1023 1085 1123 1547 1781 2366
Size: 549 186 288 62 38 424 234 585
Cuts at: 2366 2438 2715 2829
Size: 72 277 114
Fragments arranged by size:
585 549 424 288 277 234 186 114
72 62 38
BmgBI CAC'GTC
Cuts at: 0 644 1719 2829
Size: 644 1075 1110
Fragments arranged by size:
1110 1075 644
BmrI ACTGGGnnnn_n'
Cuts at: 0 2323 2391 2829
Size: 2323 68 438
Fragments arranged by size:
2323 438 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2769 2829
Size: 2769 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 796 1780 2829
Size: 214 582 984 1049
Fragments arranged by size:
1049 984 582 214
Bpu10I CC'TnA_GC
Cuts at: 0 957 1067 1645 2317 2829
Size: 957 110 578 672 512
Fragments arranged by size:
957 672 578 512 110
BsaAI yAC'GTr
Cuts at: 0 1830 2358 2829
Size: 1830 528 471
Fragments arranged by size:
1830 528 471
BsaBI GATnn'nnATC
Cuts at: 0 328 2829
Size: 328 2501
BsaHI Gr'CG_yC
Cuts at: 0 443 2829
Size: 443 2386
BsaWI w'CCGG_w
Cuts at: 0 332 563 1445 2114 2829
Size: 332 231 882 669 715
Fragments arranged by size:
882 715 669 332 231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 2829
Size: 709 30 2090
Fragments arranged by size:
2090 709 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1444 1570 2546 2752 2755 2758 2829
Size: 1444 126 976 206 3 3 71
Fragments arranged by size:
1444 976 206 126 71 3 3
BseYI C'CCAG_C
Cuts at: 0 459 915 927 1228 1342 1434 1617 1944
Size: 459 456 12 301 114 92 183 327
Cuts at: 1944 2197 2575 2775 2829
Size: 253 378 200 54
Fragments arranged by size:
459 456 378 327 301 253 200 183
114 92 54 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 328 2539 2746 2829
Size: 226 102 2211 207 83
Fragments arranged by size:
2211 226 207 102 83
BsiEI CG_ry'CG
Cuts at: 0 31 50 62 2829
Size: 31 19 12 2767
Fragments arranged by size:
2767 31 19 12
BsiHKAI G_wGCw'C
Cuts at: 0 549 670 1587 1781 2323 2722 2829
Size: 549 121 917 194 542 399 107
Fragments arranged by size:
917 549 542 399 194 121 107
BsiWI C'GTAC_G
Cuts at: 0 1149 2829
Size: 1149 1680
Bsp1286I G_dGCh'C
Cuts at: 0 13 549 670 735 883 1023 1085 1123
Size: 13 536 121 65 148 140 62 38
Cuts at: 1123 1547 1587 1766 1781 2323 2366 2438 2715
Size: 424 40 179 15 542 43 72 277
Cuts at: 2715 2722 2829
Size: 7 107
Fragments arranged by size:
542 536 424 277 179 148 140 121
107 72 65 62 43 40 38 15
13 7
BspEI T'CCGG_A
Cuts at: 0 332 2114 2829
Size: 332 1782 715
Fragments arranged by size:
1782 715 332
BspHI T'CATG_A
Cuts at: 0 2171 2829
Size: 2171 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2115 2507 2538 2787 2829
Size: 2115 392 31 249 42
Fragments arranged by size:
2115 392 249 42 31
BsrBI CCG'CTC
Cuts at: 0 815 825 2829
Size: 815 10 2004
Fragments arranged by size:
2004 815 10
BsrDI GCAATG_nn'
Cuts at: 0 1863 2343 2829
Size: 1863 480 486
Fragments arranged by size:
1863 486 480
BsrFI r'CCGG_y
Cuts at: 0 1445 2120 2201 2231 2475 2481 2829
Size: 1445 675 81 30 244 6 348
Fragments arranged by size:
1445 675 348 244 81 30 6
BsrGI T'GTAC_A
Cuts at: 0 1214 2829
Size: 1214 1615
BssHII G'CGCG_C
Cuts at: 0 721 2829
Size: 721 2108
BssSI C'ACGA_G
Cuts at: 0 505 1176 2829
Size: 505 671 1653
Fragments arranged by size:
1653 671 505
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2130 2550 2829
Size: 2130 420 279
Fragments arranged by size:
2130 420 279
BstEII G'GTnAC_C
Cuts at: 0 1468 1626 2829
Size: 1468 158 1203
Fragments arranged by size:
1468 1203 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 576 2829
Size: 511 65 2253
Fragments arranged by size:
2253 511 65
BstYI r'GATC_y
Cuts at: 0 114 1889 2522 2829
Size: 114 1775 633 307
Fragments arranged by size:
1775 633 307 114
BtgI C'CryG_G
Cuts at: 0 22 316 1015 2429 2624 2645 2829
Size: 22 294 699 1414 195 21 184
Fragments arranged by size:
1414 699 294 195 184 22 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 792 1218 1236 2829
Size: 792 426 18 1593
Fragments arranged by size:
1593 792 426 18
BtsI GCAGTG_nn'
Cuts at: 0 243 2601 2829
Size: 243 2358 228
Fragments arranged by size:
2358 243 228
DraIII CAC_nnn'GTG
Cuts at: 0 702 1785 1830 2829
Size: 702 1083 45 999
Fragments arranged by size:
1083 999 702 45
EaeI y'GGCC_r
Cuts at: 0 28 47 59 407 1511 1847 2829
Size: 28 19 12 348 1104 336 982
Fragments arranged by size:
1104 982 348 336 28 19 12
EagI C'GGCC_G
Cuts at: 0 28 47 59 2829
Size: 28 19 12 2770
Fragments arranged by size:
2770 28 19 12
EarI CTCTTCn'nnn_
Cuts at: 0 1912 2829
Size: 1912 917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 336 1743 2829
Size: 336 1407 1086
Fragments arranged by size:
1407 1086 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 214 571 796 826 1546 1641 1780
Size: 77 137 357 225 30 720 95 139
Cuts at: 1780 2040 2829
Size: 260 789
Fragments arranged by size:
789 720 357 260 225 139 137 95
77 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1552 2047 2829
Size: 1552 495 782
Fragments arranged by size:
1552 782 495
EcoO109I rG'GnC_Cy
Cuts at: 0 133 369 1020 1119 1120 1543 1544 1842
Size: 133 236 651 99 1 423 1 298
Cuts at: 1842 2362 2376 2492 2711 2829
Size: 520 14 116 219 118
Fragments arranged by size:
651 520 423 298 236 219 133 118
116 99 14 1 1
EcoRI G'AATT_C
Cuts at: 0 1687 2829
Size: 1687 1142
FspI TGC'GCA
Cuts at: 0 904 2829
Size: 904 1925
FspAI rTGC'GCAy
Cuts at: 0 904 2829
Size: 904 1925
HaeII r_GCGC'y
Cuts at: 0 19 865 2681 2829
Size: 19 846 1816 148
Fragments arranged by size:
1816 846 148 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 347 379 652 684 709 741
Size: 150 32 165 32 273 32 25 32
Cuts at: 741 1159 1191 1801 1833 2011 2043 2829
Size: 418 32 610 32 178 32 786
Fragments arranged by size:
786 610 418 273 178 165 150 32
32 32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 0 2463 2829
Size: 2463 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 490 638 842 2114 2282 2660 2829
Size: 187 303 148 204 1272 168 378 169
Fragments arranged by size:
1272 378 303 204 187 169 168 148
MscI TGG'CCA
Cuts at: 0 409 1513 1849 2829
Size: 409 1104 336 980
Fragments arranged by size:
1104 980 409 336
MslI CAynn'nnrTG
Cuts at: 0 509 574 832 1357 2407 2588 2644 2829
Size: 509 65 258 525 1050 181 56 185
Fragments arranged by size:
1050 525 509 258 185 181 65 56
MspA1I CmG'CkG
Cuts at: 0 24 33 318 622 846 1320 1329 1970
Size: 24 9 285 304 224 474 9 641
Cuts at: 1970 2820 2829
Size: 850 9
Fragments arranged by size:
850 641 474 304 285 224 24 9
9 9
NaeI GCC'GGC
Cuts at: 0 2203 2233 2483 2829
Size: 2203 30 250 346
Fragments arranged by size:
2203 346 250 30
NcoI C'CATG_G
Cuts at: 0 2429 2645 2829
Size: 2429 216 184
Fragments arranged by size:
2429 216 184
NgoMIV G'CCGG_C
Cuts at: 0 2201 2231 2481 2829
Size: 2201 30 250 348
Fragments arranged by size:
2201 348 250 30
NotI GC'GGCC_GC
Cuts at: 0 28 59 2829
Size: 28 31 2770
Fragments arranged by size:
2770 31 28
NspI r_CATG'y
Cuts at: 0 903 1281 2829
Size: 903 378 1548
Fragments arranged by size:
1548 903 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 405 1353 1509 1830 2100 2707 2829
Size: 372 33 948 156 321 270 607 122
Fragments arranged by size:
948 607 372 321 270 156 122 33
PfoI T'CCnGG_A
Cuts at: 0 85 365 2829
Size: 85 280 2464
Fragments arranged by size:
2464 280 85
PmlI CAC'GTG
Cuts at: 0 1830 2829
Size: 1830 999
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 2829
Size: 499 32 2298
Fragments arranged by size:
2298 499 32
PpuMI rG'GwC_Cy
Cuts at: 0 133 369 1842 2829
Size: 133 236 1473 987
Fragments arranged by size:
1473 987 236 133
PspOMI G'GGCC_C
Cuts at: 0 1019 1119 1543 2362 2711 2829
Size: 1019 100 424 819 349 118
Fragments arranged by size:
1019 819 424 349 118 100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1987 2019 2829
Size: 1987 32 810
Fragments arranged by size:
1987 810 32
PstI C_TGCA'G
Cuts at: 0 1028 2072 2798 2829
Size: 1028 1044 726 31
Fragments arranged by size:
1044 1028 726 31
PvuII CAG'CTG
Cuts at: 0 846 1329 2820 2829
Size: 846 483 1491 9
Fragments arranged by size:
1491 846 483 9
SacII CC_GC'GG
Cuts at: 0 25 319 2829
Size: 25 294 2510
Fragments arranged by size:
2510 294 25
SfcI C'TryA_G
Cuts at: 0 1024 2068 2794 2829
Size: 1024 1044 726 35
Fragments arranged by size:
1044 1024 726 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1984 2491 2829
Size: 1984 507 338
Fragments arranged by size:
1984 507 338
SmaI CCC'GGG
Cuts at: 0 7 730 1490 2829
Size: 7 723 760 1339
Fragments arranged by size:
1339 760 723 7
SphI G_CATG'C
Cuts at: 0 903 2829
Size: 903 1926
StyI C'CwwG_G
Cuts at: 0 222 1631 2429 2645 2829
Size: 222 1409 798 216 184
Fragments arranged by size:
1409 798 222 216 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 750 2148 2829
Size: 750 1398 681
Fragments arranged by size:
1398 750 681
TatI w'GTAC_w
Cuts at: 0 800 1214 2829
Size: 800 414 1615
Fragments arranged by size:
1615 800 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 81 113 1033 1065 2829
Size: 81 32 920 32 1764
Fragments arranged by size:
1764 920 81 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1350 1827 2097 2593 2643 2829
Size: 1350 477 270 496 50 186
Fragments arranged by size:
1350 496 477 270 186 50
XmaI C'CCGG_G
Cuts at: 0 5 728 1488 2829
Size: 5 723 760 1341
Fragments arranged by size:
1341 760 723 5
XmnI GAAnn'nnTTC
Cuts at: 0 2191 2829
Size: 2191 638
Enzymes that do cut:
AarI AccI AcuI AgeI AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NotI NspI PflMI PfoI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuII SacII SfcI SfiI SmaI SphI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AlfI AloI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI BmtI BpuEI BsaI BsmI
BsmBI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EcoICRI EcoRV FalI FseI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NruI NsiI PacI
PasI PciI PmeI PshAI PsiI PspXI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI ZraI