(Circular) (Six-base) MAPSORT of: pFN21AB9896.seq Check: 8111 from: 1 to: 8236 pFN21AB9896 8236 bp With 182 enzymes: SgfI * March 2, 2011 15:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4005 4812 4005 Size: 807 7429 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6046 278 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3657 3792 4110 4701 5579 1937 Size: 1720 135 318 591 878 4594 Fragments arranged by size: 4594 1720 878 591 318 135 Acc65I G'GTAC_C Cuts at: 4608 4944 5557 4608 Size: 336 613 7287 Fragments arranged by size: 7287 613 336 AclI AA'CG_TT Cuts at: 1563 6363 6736 1563 Size: 4800 373 3063 Fragments arranged by size: 4800 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2005 2177 3305 6299 7683 7951 1632 Size: 373 172 1128 2994 1384 268 1917 Fragments arranged by size: 2994 1917 1384 1128 373 268 172 AfeI AGC'GCT Cuts at: 3095 3095 Size: 8236 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8039 AflIII A'CryG_T Cuts at: 1203 7135 8112 1203 Size: 5932 977 1327 Fragments arranged by size: 5932 1327 977 AgeI A'CCGG_T Cuts at: 7067 7067 Size: 8236 AhdI GACnn_n'nnGTC Cuts at: 6965 6965 Size: 8236 AleI CACnn'nnGTG Cuts at: 1166 2576 2918 3326 1166 Size: 1410 342 408 6076 Fragments arranged by size: 6076 1410 408 342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2936 4077 4182 1184 Size: 1752 1141 105 5238 Fragments arranged by size: 5238 1752 1141 105 AlwNI CAG_nnn'CTG Cuts at: 2047 2276 2512 2647 2734 2765 3724 3907 4621 Size: 229 236 135 87 31 959 183 714 Cuts at: 4621 4904 5979 7551 2047 Size: 283 1075 1572 2732 Fragments arranged by size: 2732 1572 1075 959 714 283 236 229 183 135 87 31 ApaI G_GGCC'C Cuts at: 2524 3622 3718 4879 2524 Size: 1098 96 1161 5881 Fragments arranged by size: 5881 1161 1098 96 ApaLI G'TGCA_C Cuts at: 3565 6293 7449 3565 Size: 2728 1156 4352 Fragments arranged by size: 4352 2728 1156 ApoI r'AATT_y Cuts at: 1493 4589 5545 5771 5923 8032 1493 Size: 3096 956 226 152 2109 1697 Fragments arranged by size: 3096 2109 1697 956 226 152 AseI AT'TA_AT Cuts at: 160 1032 6790 160 Size: 872 5758 1606 Fragments arranged by size: 5758 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 AvaI C'yCGr_G Cuts at: 1870 1943 2627 3621 3876 4092 4604 4809 4872 Size: 73 684 994 255 216 512 205 63 Cuts at: 4872 5408 5450 5561 1870 Size: 536 42 111 4545 Fragments arranged by size: 4545 994 684 536 512 255 216 205 111 73 63 42 BamHI G'GATC_C Cuts at: 5566 5933 5566 Size: 367 7869 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2912 3686 4158 4608 4944 Size: 325 217 627 1125 774 472 450 336 Cuts at: 4944 5090 5404 5557 7012 618 Size: 146 314 153 1455 1842 Fragments arranged by size: 1842 1455 1125 774 627 472 450 336 325 314 217 153 146 BanII G_rGCy'C Cuts at: 1391 2336 2524 3132 3622 3718 4651 4879 5555 Size: 945 188 608 490 96 933 228 676 Cuts at: 5555 1391 Size: 4072 Fragments arranged by size: 4072 945 933 676 608 490 228 188 96 BbeI G_GCGC'C Cuts at: 5408 5408 Size: 8236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2190 2867 3353 3568 4280 928 Size: 971 291 677 486 215 712 4884 Fragments arranged by size: 4884 971 712 677 486 291 215 BbvCI CC'TCA_GC Cuts at: 5433 5433 Size: 8236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3175 3209 6427 6461 1903 Size: 34 1238 34 3218 34 3678 Fragments arranged by size: 3678 3218 1238 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6128 7338 6128 Size: 1210 7026 BclI T'GATC_A Cuts at: 1548 1548 Size: 8236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2756 3923 Size: 107 122 71 631 605 472 612 1167 Cuts at: 3923 4334 4343 5298 5341 6847 136 Size: 411 9 955 43 1506 1525 Fragments arranged by size: 1525 1506 1167 955 631 612 605 472 411 122 107 71 43 9 BglII A'GATC_T Cuts at: 4245 8231 4245 Size: 3986 4250 BlpI GC'TnA_GC Cuts at: 4776 5653 4776 Size: 877 7359 Bme1580I G_kGCm'C Cuts at: 1790 2270 2524 3152 3569 3622 3689 3718 3928 Size: 480 254 628 417 53 67 29 210 Cuts at: 3928 4161 4192 4330 4790 4879 5093 6297 7453 Size: 233 31 138 460 89 214 1204 1156 Cuts at: 7453 1790 Size: 2573 Fragments arranged by size: 2573 1204 1156 628 480 460 417 254 233 214 210 138 89 67 53 31 29 BmgBI CAC'GTC Cuts at: 1318 3328 1318 Size: 2010 6226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3473 3712 3728 Size: 416 53 83 399 24 2056 239 16 Cuts at: 3728 3938 4469 5967 6925 442 Size: 210 531 1498 958 1753 Fragments arranged by size: 2056 1753 1498 958 531 416 399 239 210 83 53 24 16 BmtI G_CTAG'C Cuts at: 1056 4083 1056 Size: 3027 5209 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2355 2355 Size: 8236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3862 5345 6896 1239 Size: 2623 1483 1551 2579 Fragments arranged by size: 2623 2579 1551 1483 Bpu10I CC'TnA_GC Cuts at: 2354 4319 5147 5433 2354 Size: 1965 828 286 5157 Fragments arranged by size: 5157 1965 828 286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3218 5141 6361 7226 7524 3116 Size: 102 1923 1220 865 298 3828 Fragments arranged by size: 3828 1923 1220 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2897 2911 4825 6899 882 Size: 617 1398 14 1914 2074 2219 Fragments arranged by size: 2219 2074 1914 1398 617 14 BsaAI yAC'GTr Cuts at: 493 1204 6027 493 Size: 711 4823 2702 Fragments arranged by size: 4823 2702 711 BsaBI GATnn'nnATC Cuts at: 8230 8230 Size: 8236 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 5405 6043 6425 Size: 53 83 186 936 603 3269 638 382 Cuts at: 6425 7060 275 Size: 635 1451 Fragments arranged by size: 3269 1451 936 638 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6669 7067 7341 7488 7899 6669 Size: 398 274 147 411 7006 Fragments arranged by size: 7006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2862 2892 3089 3119 4439 Size: 30 165 30 1459 30 197 30 1320 Cuts at: 4439 4469 4649 4679 5244 5274 5298 5328 1178 Size: 30 180 30 565 30 24 30 4086 Fragments arranged by size: 4086 1459 1320 565 197 180 165 30 30 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2326 3094 3965 4781 5252 1186 Size: 1140 768 871 816 471 4170 Fragments arranged by size: 4170 1140 871 816 768 471 BseYI C'CCAG_C Cuts at: 2047 2249 2394 2542 2548 2591 2637 2724 3613 Size: 202 145 148 6 43 46 87 889 Cuts at: 3613 3632 3682 3711 3900 4548 4559 4882 5077 Size: 19 50 29 189 648 11 323 195 Cuts at: 5077 7439 7935 2047 Size: 2362 496 2348 Fragments arranged by size: 2362 2348 889 648 496 323 202 195 189 148 145 87 50 46 43 29 19 11 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 2942 3509 4106 4115 4230 4838 Size: 573 649 557 567 597 9 115 608 Cuts at: 4838 5021 5462 1163 Size: 183 441 3937 Fragments arranged by size: 3937 649 608 597 573 567 557 441 183 115 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3518 5269 5467 5686 6447 7475 Size: 275 186 1517 1751 198 219 761 1028 Cuts at: 7475 1540 Size: 2301 Fragments arranged by size: 2301 1751 1517 1028 761 275 219 198 186 BsiHKAI G_wGCw'C Cuts at: 2336 3132 3333 3569 3768 4066 4434 4534 4803 Size: 796 201 236 199 298 368 100 269 Cuts at: 4803 5352 6297 6382 7453 2336 Size: 549 945 85 1071 3119 Fragments arranged by size: 3119 1071 945 796 549 368 298 269 236 201 199 100 85 BsmI GAATG_Cn' Cuts at: 5747 5840 5747 Size: 93 8143 BsmBI CGTCTCn'nnnn_ Cuts at: 2695 3525 3775 4013 4089 2695 Size: 830 250 238 76 6842 Fragments arranged by size: 6842 830 250 238 76 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2336 2524 3132 3152 3333 3569 Size: 399 480 66 188 608 20 181 236 Cuts at: 3569 3622 3689 3718 3768 3928 4066 4161 4192 Size: 53 67 29 50 160 138 95 31 Cuts at: 4192 4330 4434 4534 4651 4790 4803 4879 5093 Size: 138 104 100 117 139 13 76 214 Cuts at: 5093 5352 5555 6297 6382 7453 1391 Size: 259 203 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 608 480 399 259 236 214 203 188 181 160 139 138 138 117 104 100 95 85 76 67 66 53 50 31 29 20 13 BspEI T'CCGG_A Cuts at: 7899 7899 Size: 8236 BspHI T'CATG_A Cuts at: 6125 7855 6125 Size: 1730 6506 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2788 2879 4005 4620 4812 4956 5028 5391 Size: 1944 91 1126 615 192 144 72 363 Cuts at: 5391 5591 844 Size: 200 3489 Fragments arranged by size: 3489 1944 1126 615 363 200 192 144 91 72 BsrBI CCG'CTC Cuts at: 1394 1823 2358 3102 3549 4780 6123 1394 Size: 429 535 744 447 1231 1343 3507 Fragments arranged by size: 3507 1343 1231 744 535 447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6731 6905 65 Size: 1183 5483 174 1396 Fragments arranged by size: 5483 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3012 3933 4694 6880 7067 1700 Size: 110 1202 921 761 2186 187 2869 Fragments arranged by size: 2869 2186 1202 921 761 187 110 BsrGI T'GTAC_A Cuts at: 96 4455 96 Size: 4359 3877 BssHII G'CGCG_C Cuts at: 1925 5441 1925 Size: 3516 4720 BssSI C'ACGA_G Cuts at: 6296 7308 6296 Size: 1012 7224 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 4129 4208 4217 794 Size: 1853 1482 79 9 4813 Fragments arranged by size: 4813 1853 1482 79 9 BstBI TT'CG_AA Cuts at: 2181 7040 2181 Size: 4859 3377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8236 BstXI CCAn_nnnn'nTGG Cuts at: 2131 3056 3668 3741 4177 2131 Size: 925 612 73 436 6190 Fragments arranged by size: 6190 925 612 436 73 BstYI r'GATC_y Cuts at: 1972 2790 3136 3289 4245 5566 5933 6318 6335 Size: 818 346 153 956 1321 367 385 17 Cuts at: 6335 7787 7873 7885 8231 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1321 956 818 385 367 346 346 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5156 5156 Size: 8236 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 2754 3055 4137 4286 4392 Size: 552 939 162 588 301 1082 149 106 Cuts at: 4392 4442 5214 5330 513 Size: 50 772 116 3419 Fragments arranged by size: 3419 1082 939 772 588 552 301 162 149 116 106 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3833 487 Size: 683 56 2607 4890 Fragments arranged by size: 4890 2607 683 56 BtsI GCAGTG_nn' Cuts at: 3258 3316 3442 3457 3463 4164 4330 4382 5752 Size: 58 126 15 6 701 166 52 1370 Cuts at: 5752 6546 6566 3258 Size: 794 20 4928 Fragments arranged by size: 4928 1370 794 701 166 126 58 52 20 15 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4275 4310 529 Size: 35 3711 35 4455 Fragments arranged by size: 4455 3711 35 35 DraI TTT'AAA Cuts at: 5540 5540 Size: 8236 DraIII CAC_nnn'GTG Cuts at: 4352 4393 4352 Size: 41 8195 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3479 7243 809 Size: 507 2163 3764 1802 Fragments arranged by size: 3764 2163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3515 4239 4464 Size: 54 1425 50 275 18 1685 724 225 Cuts at: 4464 4683 4710 5683 6572 8201 8 Size: 219 27 973 889 1629 43 Fragments arranged by size: 1685 1629 1425 973 889 724 275 225 219 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3515 5683 1537 Size: 275 1703 2168 4090 Fragments arranged by size: 4090 2168 1703 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 6166 1353 Size: 1107 3706 3423 Fragments arranged by size: 3706 3423 1107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2970 5104 5333 6823 7197 7343 Size: 1064 138 1381 2134 229 1490 374 146 Cuts at: 7343 387 Size: 1280 Fragments arranged by size: 2134 1490 1381 1280 1064 374 229 146 138 EcoICRI GAG'CTC Cuts at: 2334 3130 2334 Size: 796 7440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2177 3305 3862 5345 6299 6896 Size: 393 373 172 1128 557 1483 954 597 Cuts at: 6896 7683 7951 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1483 1128 954 787 597 557 393 373 268 172 EcoNI CCTnn'n_nnAGG Cuts at: 2580 2601 4052 4490 5207 2580 Size: 21 1451 438 717 5609 Fragments arranged by size: 5609 1451 717 438 21 EcoO109I rG'GnC_Cy Cuts at: 2520 2521 2680 2800 2930 3034 3618 3824 4045 Size: 1 159 120 130 104 584 206 221 Cuts at: 4045 4876 5103 5143 5291 2520 Size: 831 227 40 148 5465 Fragments arranged by size: 5465 831 584 227 221 206 159 148 130 120 104 40 1 EcoRI G'AATT_C Cuts at: 4589 5545 5923 4589 Size: 956 378 6902 Fragments arranged by size: 6902 956 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2207 2207 Size: 8236 FseI GG_CCGG'CC Cuts at: 3937 3937 Size: 8236 FspI TGC'GCA Cuts at: 3185 3257 5036 6742 3185 Size: 72 1779 1706 4679 Fragments arranged by size: 4679 1779 1706 72 FspAI rTGC'GCAy Cuts at: 5036 5036 Size: 8236 HaeII r_GCGC'y Cuts at: 1678 2048 2078 2375 3097 4086 4092 4184 5408 Size: 370 30 297 722 989 6 92 1224 Cuts at: 5408 7383 1678 Size: 1975 2531 Fragments arranged by size: 2531 1975 1224 989 722 370 297 92 30 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 3281 3313 3377 Size: 32 135 32 707 32 970 32 64 Cuts at: 3377 3409 4208 4240 6877 6909 6951 6983 1373 Size: 32 799 32 2637 32 42 32 2626 Fragments arranged by size: 2637 2626 970 799 707 135 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5580 5826 669 Size: 988 281 3642 246 3079 Fragments arranged by size: 3642 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5456 748 Size: 4708 3528 HpaI GTT'AAC Cuts at: 5826 5826 Size: 8236 KasI G'GCGC_C Cuts at: 5404 5404 Size: 8236 KpnI G_GTAC'C Cuts at: 4612 4948 5561 4612 Size: 336 613 7287 Fragments arranged by size: 7287 613 336 MfeI C'AATT_G Cuts at: 5835 5835 Size: 8236 MluI A'CGCG_T Cuts at: 8112 8112 Size: 8236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5161 7350 7534 1305 Size: 3856 2189 184 2007 Fragments arranged by size: 3856 2189 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4685 4712 10 Size: 54 1425 343 2853 27 3534 Fragments arranged by size: 3534 2853 1425 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2576 2918 3326 6194 6553 6712 518 Size: 648 1410 342 408 2868 359 159 2042 Fragments arranged by size: 2868 2042 1410 648 408 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2647 2747 3023 3444 3514 3907 4023 4129 Size: 1041 100 276 421 70 393 116 106 Cuts at: 4129 4574 4618 4629 4736 4792 5005 5332 5652 Size: 445 44 11 107 56 213 327 320 Cuts at: 5652 6329 7477 7722 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1041 677 445 421 393 327 320 276 245 213 116 107 106 100 70 56 44 11 NaeI GCC'GGC Cuts at: 3935 3935 Size: 8236 NarI GG'CG_CC Cuts at: 5405 5405 Size: 8236 NcoI C'CATG_G Cuts at: 513 1065 2004 2166 4137 4442 513 Size: 552 939 162 1971 305 4307 Fragments arranged by size: 4307 1971 939 552 305 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 8236 NgoMIV G'CCGG_C Cuts at: 3933 3933 Size: 8236 NheI G'CTAG_C Cuts at: 1052 4079 1052 Size: 3027 5209 NotI GC'GGCC_GC Cuts at: 5683 5683 Size: 8236 NspI r_CATG'y Cuts at: 1132 1229 2191 3541 5428 5594 7139 1132 Size: 97 962 1350 1887 166 1545 2229 Fragments arranged by size: 2229 1887 1545 1350 962 166 97 PasI CC'CwG_GG Cuts at: 1348 2447 3668 4565 4842 5086 1348 Size: 1099 1221 897 277 244 4498 Fragments arranged by size: 4498 1221 1099 897 277 244 PciI A'CATG_T Cuts at: 7135 7135 Size: 8236 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 4377 4393 4719 5046 1108 Size: 1608 1661 16 326 327 4298 Fragments arranged by size: 4298 1661 1608 327 326 16 PfoI T'CCnGG_A Cuts at: 2599 4509 2599 Size: 1910 6326 PmeI GTTT'AAAC Cuts at: 5540 5540 Size: 8236 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6305 6337 7818 7850 6305 Size: 32 1481 32 6691 Fragments arranged by size: 6691 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2680 2800 5143 2680 Size: 120 2343 5773 Fragments arranged by size: 5773 2343 120 PshAI GACnn'nnGTC Cuts at: 1613 5271 5522 1613 Size: 3658 251 4327 Fragments arranged by size: 4327 3658 251 PsiI TTA'TAA Cuts at: 5806 5806 Size: 8236 PspOMI G'GGCC_C Cuts at: 2520 3618 3714 4875 2520 Size: 1098 96 1161 5881 Fragments arranged by size: 5881 1161 1098 96 PstI C_TGCA'G Cuts at: 830 2493 2817 4005 4053 4617 4821 4953 5588 Size: 1663 324 1188 48 564 204 132 635 Cuts at: 5588 830 Size: 3478 Fragments arranged by size: 3478 1663 1188 635 564 324 204 132 48 PvuI CG_AT'CG Cuts at: 2001 5467 2001 Size: 3466 4770 PvuII CAG'CTG Cuts at: 2747 3023 3907 4129 4574 4618 4629 4736 5005 Size: 276 884 222 445 44 11 107 269 Cuts at: 5005 2747 Size: 5978 Fragments arranged by size: 5978 884 445 276 269 222 107 44 11 SacI G_AGCT'C Cuts at: 2336 3132 2336 Size: 796 7440 SacII CC_GC'GG Cuts at: 5333 5333 Size: 8236 SalI G'TCGA_C Cuts at: 1936 5578 1936 Size: 3642 4594 SanDI GG'GwC_CC Cuts at: 2680 2680 Size: 8236 SbfI CC_TGCA'GG Cuts at: 4953 5588 4953 Size: 635 7601 ScaI AGT'ACT Cuts at: 6484 8196 6484 Size: 1712 6524 SfcI C'TryA_G Cuts at: 826 1045 2489 2613 2813 4001 4049 4613 4657 Size: 219 1444 124 200 1188 48 564 44 Cuts at: 4657 4817 4949 5584 6719 7400 7591 826 Size: 160 132 635 1135 681 191 1471 Fragments arranged by size: 1471 1444 1188 1135 681 635 564 219 200 191 160 132 124 48 44 SfoI GGC'GCC Cuts at: 5406 5406 Size: 8236 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 SgrAI Cr'CCGG_yG Cuts at: 1700 4694 1700 Size: 2994 5242 SmaI CCC'GGG Cuts at: 1872 2629 3623 3878 4811 4874 5410 5563 1872 Size: 757 994 255 933 63 536 153 4545 Fragments arranged by size: 4545 994 933 757 536 255 153 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 3233 5120 5450 6340 7241 Size: 197 926 1188 102 1887 330 890 901 Cuts at: 7241 7503 820 Size: 262 1553 Fragments arranged by size: 1887 1553 1188 926 901 890 330 262 197 102 SnaBI TAC'GTA Cuts at: 493 6027 493 Size: 5534 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8236 SphI G_CATG'C Cuts at: 1132 3541 5428 5594 1132 Size: 2409 1887 166 3774 Fragments arranged by size: 3774 2409 1887 166 SrfI GCCC'GGGC Cuts at: 4874 5410 4874 Size: 536 7700 SspI AAT'ATT Cuts at: 5 52 6160 5 Size: 47 6108 2081 Fragments arranged by size: 6108 2081 47 StuI AGG'CCT Cuts at: 1517 4337 5360 1517 Size: 2820 1023 4393 Fragments arranged by size: 4393 2820 1023 StyI C'CwwG_G Cuts at: 513 1065 2004 2141 2166 3421 3600 3774 4137 Size: 552 939 137 25 1255 179 174 363 Cuts at: 4137 4442 4893 5675 513 Size: 305 451 782 3074 Fragments arranged by size: 3074 1255 939 782 552 451 363 305 179 174 137 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2099 2419 2651 2717 4003 6263 6280 Size: 1255 519 320 232 66 1286 2260 17 Cuts at: 6280 6433 6618 7063 325 Size: 153 185 445 1498 Fragments arranged by size: 2260 1498 1286 1255 519 445 320 232 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3834 4455 6482 Size: 274 80 33 51 1443 1857 621 2027 Cuts at: 6482 8194 96 Size: 1712 138 Fragments arranged by size: 2027 1857 1712 1443 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 2523 2555 2862 2894 4299 4331 5160 Size: 32 22 32 307 32 1405 32 829 Cuts at: 5160 5192 5244 5276 5298 5330 8196 8228 2469 Size: 32 52 32 22 32 2866 32 2477 Fragments arranged by size: 2866 2477 1405 829 307 52 32 32 32 32 32 32 32 32 22 22 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8236 XbaI T'CTAG_A Cuts at: 5572 5572 Size: 8236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3952 3952 Size: 8236 XhoI C'TCGA_G Cuts at: 1943 5450 1943 Size: 3507 4729 XmaI C'CCGG_G Cuts at: 1870 2627 3621 3876 4809 4872 5408 5561 1870 Size: 757 994 255 933 63 536 153 4545 Fragments arranged by size: 4545 994 933 757 536 255 153 63 XmnI GAAnn'nnTTC Cuts at: 6365 6365 Size: 8236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6044 276 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SwaI