(Linear) (Six-base) MAPSORT of: KIBB9896.seq Check: 7275 from: 1 to: 3531 KIBB9896.orf With 182 enzymes: SgfI * March 2, 2011 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2000 2807 3531 Size: 2000 807 724 Fragments arranged by size: 2000 807 724 AccI GT'mk_AC Cuts at: 0 1652 1787 2105 2696 3531 Size: 1652 135 318 591 835 Fragments arranged by size: 1652 835 591 318 135 Acc65I G'GTAC_C Cuts at: 0 2603 2939 3531 Size: 2603 336 592 Fragments arranged by size: 2603 592 336 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1300 3531 Size: 172 1128 2231 Fragments arranged by size: 2231 1128 172 AfeI AGC'GCT Cuts at: 0 1090 3531 Size: 1090 2441 AleI CACnn'nnGTG Cuts at: 0 571 913 1321 3531 Size: 571 342 408 2210 Fragments arranged by size: 2210 571 408 342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 931 2072 2177 3531 Size: 931 1141 105 1354 Fragments arranged by size: 1354 1141 931 105 AlwNI CAG_nnn'CTG Cuts at: 0 42 271 507 642 729 760 1719 1902 Size: 42 229 236 135 87 31 959 183 Cuts at: 1902 2616 2899 3531 Size: 714 283 632 Fragments arranged by size: 959 714 632 283 236 229 183 135 87 42 31 ApaI G_GGCC'C Cuts at: 0 519 1617 1713 2874 3531 Size: 519 1098 96 1161 657 Fragments arranged by size: 1161 1098 657 519 96 ApaLI G'TGCA_C Cuts at: 0 1560 3531 Size: 1560 1971 ApoI r'AATT_y Cuts at: 0 2584 3531 Size: 2584 947 AvaI C'yCGr_G Cuts at: 0 622 1616 1871 2087 2599 2804 2867 3403 Size: 622 994 255 216 512 205 63 536 Cuts at: 3403 3445 3531 Size: 42 86 Fragments arranged by size: 994 622 536 512 255 216 205 86 63 42 BanI G'GyrC_C Cuts at: 0 907 1681 2153 2603 2939 3085 3399 3531 Size: 907 774 472 450 336 146 314 132 Fragments arranged by size: 907 774 472 450 336 314 146 132 BanII G_rGCy'C Cuts at: 0 331 519 1127 1617 1713 2646 2874 3531 Size: 331 188 608 490 96 933 228 657 Fragments arranged by size: 933 657 608 490 331 228 188 96 BbeI G_GCGC'C Cuts at: 0 3403 3531 Size: 3403 128 BbsI GAAGACnn'nnnn_ Cuts at: 0 185 862 1348 1563 2275 3531 Size: 185 677 486 215 712 1256 Fragments arranged by size: 1256 712 677 486 215 185 BbvCI CC'TCA_GC Cuts at: 0 3428 3531 Size: 3428 103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1170 1204 3531 Size: 1170 34 2327 Fragments arranged by size: 2327 1170 34 BglI GCCn_nnn'nGGC Cuts at: 0 139 751 1918 2329 2338 3293 3336 3531 Size: 139 612 1167 411 9 955 43 195 Fragments arranged by size: 1167 955 612 411 195 139 43 9 BglII A'GATC_T Cuts at: 0 2240 3531 Size: 2240 1291 BlpI GC'TnA_GC Cuts at: 0 2771 3531 Size: 2771 760 Bme1580I G_kGCm'C Cuts at: 0 265 519 1147 1564 1617 1684 1713 1923 Size: 265 254 628 417 53 67 29 210 Cuts at: 1923 2156 2187 2325 2785 2874 3088 3531 Size: 233 31 138 460 89 214 443 Fragments arranged by size: 628 460 443 417 265 254 233 214 210 138 89 67 53 31 29 BmgBI CAC'GTC Cuts at: 0 1323 3531 Size: 1323 2208 BmrI ACTGGGnnnn_n' Cuts at: 0 1468 1707 1723 1933 2464 3531 Size: 1468 239 16 210 531 1067 Fragments arranged by size: 1468 1067 531 239 210 16 BmtI G_CTAG'C Cuts at: 0 2078 3531 Size: 2078 1453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 350 3531 Size: 350 3181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1857 3340 3531 Size: 1857 1483 191 Fragments arranged by size: 1857 1483 191 Bpu10I CC'TnA_GC Cuts at: 0 349 2314 3142 3428 3531 Size: 349 1965 828 286 103 Fragments arranged by size: 1965 828 349 286 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1213 3136 3531 Size: 1111 102 1923 395 Fragments arranged by size: 1923 1111 395 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 892 906 2820 3531 Size: 892 14 1914 711 Fragments arranged by size: 1914 892 711 14 BsaHI Gr'CG_yC Cuts at: 0 131 3400 3531 Size: 131 3269 131 Fragments arranged by size: 3269 131 131 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 857 887 1084 1114 2434 2464 2644 2674 Size: 857 30 197 30 1320 30 180 30 Cuts at: 2674 3239 3269 3293 3323 3531 Size: 565 30 24 30 208 Fragments arranged by size: 1320 857 565 208 197 180 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 321 1089 1960 2776 3247 3531 Size: 321 768 871 816 471 284 Fragments arranged by size: 871 816 768 471 321 284 BseYI C'CCAG_C Cuts at: 0 42 244 389 537 543 586 632 719 Size: 42 202 145 148 6 43 46 87 Cuts at: 719 1608 1627 1677 1706 1895 2543 2554 2877 Size: 889 19 50 29 189 648 11 323 Cuts at: 2877 3072 3531 Size: 195 459 Fragments arranged by size: 889 648 459 323 202 195 189 148 145 87 50 46 43 42 29 19 11 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 937 1504 2101 2110 2225 2833 3016 Size: 380 557 567 597 9 115 608 183 Cuts at: 3016 3457 3531 Size: 441 74 Fragments arranged by size: 608 597 567 557 441 380 183 115 74 9 BsiEI CG_ry'CG Cuts at: 0 1513 3264 3462 3531 Size: 1513 1751 198 69 Fragments arranged by size: 1751 1513 198 69 BsiHKAI G_wGCw'C Cuts at: 0 331 1127 1328 1564 1763 2061 2429 2529 Size: 331 796 201 236 199 298 368 100 Cuts at: 2529 2798 3347 3531 Size: 269 549 184 Fragments arranged by size: 796 549 368 331 298 269 236 201 199 184 100 BsmBI CGTCTCn'nnnn_ Cuts at: 0 690 1520 1770 2008 2084 3531 Size: 690 830 250 238 76 1447 Fragments arranged by size: 1447 830 690 250 238 76 Bsp1286I G_dGCh'C Cuts at: 0 265 331 519 1127 1147 1328 1564 1617 Size: 265 66 188 608 20 181 236 53 Cuts at: 1617 1684 1713 1763 1923 2061 2156 2187 2325 Size: 67 29 50 160 138 95 31 138 Cuts at: 2325 2429 2529 2646 2785 2798 2874 3088 3347 Size: 104 100 117 139 13 76 214 259 Cuts at: 3347 3531 Size: 184 Fragments arranged by size: 608 265 259 236 214 188 184 181 160 139 138 138 117 104 100 95 76 67 66 53 50 31 29 20 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 783 874 2000 2615 2807 2951 3023 3386 Size: 783 91 1126 615 192 144 72 363 Cuts at: 3386 3531 Size: 145 Fragments arranged by size: 1126 783 615 363 192 145 144 91 72 BsrBI CCG'CTC Cuts at: 0 353 1097 1544 2775 3531 Size: 353 744 447 1231 756 Fragments arranged by size: 1231 756 744 447 353 BsrFI r'CCGG_y Cuts at: 0 1007 1928 2689 3531 Size: 1007 921 761 842 Fragments arranged by size: 1007 921 842 761 BsrGI T'GTAC_A Cuts at: 0 2450 3531 Size: 2450 1081 BssHII G'CGCG_C Cuts at: 0 3436 3531 Size: 3436 95 BstAPI GCAn_nnn'nTGC Cuts at: 0 642 2124 2203 2212 3531 Size: 642 1482 79 9 1319 Fragments arranged by size: 1482 1319 642 79 9 BstBI TT'CG_AA Cuts at: 0 176 3531 Size: 176 3355 BstXI CCAn_nnnn'nTGG Cuts at: 0 126 1051 1663 1736 2172 3531 Size: 126 925 612 73 436 1359 Fragments arranged by size: 1359 925 612 436 126 73 BstYI r'GATC_y Cuts at: 0 785 1131 1284 2240 3531 Size: 785 346 153 956 1291 Fragments arranged by size: 1291 956 785 346 153 Bsu36I CC'TnA_GG Cuts at: 0 3151 3531 Size: 3151 380 BtgI C'CryG_G Cuts at: 0 161 749 1050 2132 2281 2387 2437 3209 Size: 161 588 301 1082 149 106 50 772 Cuts at: 3209 3325 3531 Size: 116 206 Fragments arranged by size: 1082 772 588 301 206 161 149 116 106 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1828 3531 Size: 1828 1703 BtsI GCAGTG_nn' Cuts at: 0 1253 1311 1437 1452 1458 2159 2325 2377 Size: 1253 58 126 15 6 701 166 52 Cuts at: 2377 3531 Size: 1154 Fragments arranged by size: 1253 1154 701 166 126 58 52 15 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2270 2305 3531 Size: 2270 35 1226 Fragments arranged by size: 2270 1226 35 DraIII CAC_nnn'GTG Cuts at: 0 2347 2388 3531 Size: 2347 41 1143 Fragments arranged by size: 2347 1143 41 DrdI GACnn_nn'nnGTC Cuts at: 0 1474 3531 Size: 1474 2057 EaeI y'GGCC_r Cuts at: 0 1510 2234 2459 2678 2705 3531 Size: 1510 724 225 219 27 826 Fragments arranged by size: 1510 826 724 225 219 27 EagI C'GGCC_G Cuts at: 0 1510 3531 Size: 1510 2021 EarI CTCTTCn'nnn_ Cuts at: 0 455 3531 Size: 455 3076 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 965 3099 3328 3531 Size: 965 2134 229 203 Fragments arranged by size: 2134 965 229 203 EcoICRI GAG'CTC Cuts at: 0 329 1125 3531 Size: 329 796 2406 Fragments arranged by size: 2406 796 329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1300 1857 3340 3531 Size: 172 1128 557 1483 191 Fragments arranged by size: 1483 1128 557 191 172 EcoNI CCTnn'n_nnAGG Cuts at: 0 575 596 2047 2485 3202 3531 Size: 575 21 1451 438 717 329 Fragments arranged by size: 1451 717 575 438 329 21 EcoO109I rG'GnC_Cy Cuts at: 0 515 516 675 795 925 1029 1613 1819 Size: 515 1 159 120 130 104 584 206 Cuts at: 1819 2040 2871 3098 3138 3286 3531 Size: 221 831 227 40 148 245 Fragments arranged by size: 831 584 515 245 227 221 206 159 148 130 120 104 40 1 EcoRI G'AATT_C Cuts at: 0 2584 3531 Size: 2584 947 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 202 3531 Size: 202 3329 FseI GG_CCGG'CC Cuts at: 0 1932 3531 Size: 1932 1599 FspI TGC'GCA Cuts at: 0 1180 1252 3031 3531 Size: 1180 72 1779 500 Fragments arranged by size: 1779 1180 500 72 FspAI rTGC'GCAy Cuts at: 0 3031 3531 Size: 3031 500 HaeII r_GCGC'y Cuts at: 0 43 73 370 1092 2081 2087 2179 3403 Size: 43 30 297 722 989 6 92 1224 Cuts at: 3403 3531 Size: 128 Fragments arranged by size: 1224 989 722 297 128 92 43 30 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 274 306 1276 1308 1372 1404 2203 2235 Size: 274 32 970 32 64 32 799 32 Cuts at: 2235 3531 Size: 1296 Fragments arranged by size: 1296 970 799 274 64 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 3451 3531 Size: 3451 80 KasI G'GCGC_C Cuts at: 0 3399 3531 Size: 3399 132 KpnI G_GTAC'C Cuts at: 0 2607 2943 3531 Size: 2607 336 588 Fragments arranged by size: 2607 588 336 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3156 3531 Size: 3156 375 MscI TGG'CCA Cuts at: 0 2680 2707 3531 Size: 2680 27 824 Fragments arranged by size: 2680 824 27 MslI CAynn'nnrTG Cuts at: 0 571 913 1321 3531 Size: 571 342 408 2210 Fragments arranged by size: 2210 571 408 342 MspA1I CmG'CkG Cuts at: 0 642 742 1018 1439 1509 1902 2018 2124 Size: 642 100 276 421 70 393 116 106 Cuts at: 2124 2569 2613 2624 2731 2787 3000 3327 3531 Size: 445 44 11 107 56 213 327 204 Fragments arranged by size: 642 445 421 393 327 276 213 204 116 107 106 100 70 56 44 11 NaeI GCC'GGC Cuts at: 0 1930 3531 Size: 1930 1601 NarI GG'CG_CC Cuts at: 0 3400 3531 Size: 3400 131 NcoI C'CATG_G Cuts at: 0 161 2132 2437 3531 Size: 161 1971 305 1094 Fragments arranged by size: 1971 1094 305 161 NgoMIV G'CCGG_C Cuts at: 0 1928 3531 Size: 1928 1603 NheI G'CTAG_C Cuts at: 0 2074 3531 Size: 2074 1457 NspI r_CATG'y Cuts at: 0 186 1536 3423 3531 Size: 186 1350 1887 108 Fragments arranged by size: 1887 1350 186 108 PasI CC'CwG_GG Cuts at: 0 442 1663 2560 2837 3081 3531 Size: 442 1221 897 277 244 450 Fragments arranged by size: 1221 897 450 442 277 244 PflMI CCAn_nnn'nTGG Cuts at: 0 711 2372 2388 2714 3041 3531 Size: 711 1661 16 326 327 490 Fragments arranged by size: 1661 711 490 327 326 16 PfoI T'CCnGG_A Cuts at: 0 594 2504 3531 Size: 594 1910 1027 Fragments arranged by size: 1910 1027 594 PpuMI rG'GwC_Cy Cuts at: 0 675 795 3138 3531 Size: 675 120 2343 393 Fragments arranged by size: 2343 675 393 120 PshAI GACnn'nnGTC Cuts at: 0 3266 3517 3531 Size: 3266 251 14 Fragments arranged by size: 3266 251 14 PspOMI G'GGCC_C Cuts at: 0 515 1613 1709 2870 3531 Size: 515 1098 96 1161 661 Fragments arranged by size: 1161 1098 661 515 96 PstI C_TGCA'G Cuts at: 0 488 812 2000 2048 2612 2816 2948 3531 Size: 488 324 1188 48 564 204 132 583 Fragments arranged by size: 1188 583 564 488 324 204 132 48 PvuI CG_AT'CG Cuts at: 0 3462 3531 Size: 3462 69 PvuII CAG'CTG Cuts at: 0 742 1018 1902 2124 2569 2613 2624 2731 Size: 742 276 884 222 445 44 11 107 Cuts at: 2731 3000 3531 Size: 269 531 Fragments arranged by size: 884 742 531 445 276 269 222 107 44 11 SacI G_AGCT'C Cuts at: 0 331 1127 3531 Size: 331 796 2404 Fragments arranged by size: 2404 796 331 SacII CC_GC'GG Cuts at: 0 3328 3531 Size: 3328 203 SanDI GG'GwC_CC Cuts at: 0 675 3531 Size: 675 2856 SbfI CC_TGCA'GG Cuts at: 0 2948 3531 Size: 2948 583 SfcI C'TryA_G Cuts at: 0 484 608 808 1996 2044 2608 2652 2812 Size: 484 124 200 1188 48 564 44 160 Cuts at: 2812 2944 3531 Size: 132 587 Fragments arranged by size: 1188 587 564 484 200 160 132 124 48 44 SfoI GGC'GCC Cuts at: 0 3401 3531 Size: 3401 130 SgrAI Cr'CCGG_yG Cuts at: 0 2689 3531 Size: 2689 842 SmaI CCC'GGG Cuts at: 0 624 1618 1873 2806 2869 3405 3531 Size: 624 994 255 933 63 536 126 Fragments arranged by size: 994 933 624 536 255 126 63 SmlI C'TyrA_G Cuts at: 0 1126 1228 3115 3445 3531 Size: 1126 102 1887 330 86 Fragments arranged by size: 1887 1126 330 102 86 SphI G_CATG'C Cuts at: 0 1536 3423 3531 Size: 1536 1887 108 Fragments arranged by size: 1887 1536 108 SrfI GCCC'GGGC Cuts at: 0 2869 3405 3531 Size: 2869 536 126 Fragments arranged by size: 2869 536 126 StuI AGG'CCT Cuts at: 0 2332 3355 3531 Size: 2332 1023 176 Fragments arranged by size: 2332 1023 176 StyI C'CwwG_G Cuts at: 0 136 161 1416 1595 1769 2132 2437 2888 Size: 136 25 1255 179 174 363 305 451 Cuts at: 2888 3531 Size: 643 Fragments arranged by size: 1255 643 451 363 305 179 174 136 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 94 414 646 712 1998 3531 Size: 94 320 232 66 1286 1533 Fragments arranged by size: 1533 1286 320 232 94 66 TatI w'GTAC_w Cuts at: 0 1829 2450 3531 Size: 1829 621 1081 Fragments arranged by size: 1829 1081 621 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 464 496 518 550 857 889 2294 2326 Size: 464 32 22 32 307 32 1405 32 Cuts at: 2326 3155 3187 3239 3271 3293 3325 3531 Size: 829 32 52 32 22 32 206 Fragments arranged by size: 1405 829 464 307 206 52 32 32 32 32 32 32 32 22 22 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1947 3531 Size: 1947 1584 XhoI C'TCGA_G Cuts at: 0 3445 3531 Size: 3445 86 XmaI C'CCGG_G Cuts at: 0 622 1616 1871 2804 2867 3403 3531 Size: 622 994 255 933 63 536 128 Fragments arranged by size: 994 933 622 536 255 128 63 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuI PvuII SacI SacII SanDI SbfI SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BssSI BstEII BstZ17I ClaI DraI EcoRV HincII HpaI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI RsrII SalI SapI ScaI SexAI SfiI SgfI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI ZraI