(Circular) (Six-base) MAPSORT of: pFN21AE2322.seq Check: 422 from: 1 to: 8710 pFN21AE2322 8710 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3307 3307 Size: 8710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6520 278 Size: 53 83 186 936 4984 2468 Fragments arranged by size: 4984 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 5580 6053 1937 Size: 646 2997 473 4594 Fragments arranged by size: 4594 2997 646 473 Acc65I G'GTAC_C Cuts at: 6031 6031 Size: 8710 AclI AA'CG_TT Cuts at: 1563 6837 7210 1563 Size: 5274 373 3063 Fragments arranged by size: 5274 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2998 3455 3650 5738 6773 8157 8425 1632 Size: 1366 457 195 2088 1035 1384 268 1917 Fragments arranged by size: 2088 1917 1384 1366 1035 457 268 195 AfeI AGC'GCT Cuts at: 3802 3802 Size: 8710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8513 AflIII A'CryG_T Cuts at: 1203 7609 8586 1203 Size: 6406 977 1327 Fragments arranged by size: 6406 1327 977 AgeI A'CCGG_T Cuts at: 7541 7541 Size: 8710 AhdI GACnn_n'nnGTC Cuts at: 7439 7439 Size: 8710 AleI CACnn'nnGTG Cuts at: 1166 3881 1166 Size: 2715 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4476 1184 Size: 3292 5418 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4961 4993 4961 Size: 32 8678 AlwNI CAG_nnn'CTG Cuts at: 2653 3322 3756 4228 5142 5289 6453 8025 2653 Size: 669 434 472 914 147 1164 1572 3338 Fragments arranged by size: 3338 1572 1164 914 669 472 434 147 ApaI G_GGCC'C Cuts at: 2356 4805 2356 Size: 2449 6261 ApaLI G'TGCA_C Cuts at: 2247 3335 4141 4610 6767 7923 2247 Size: 1088 806 469 2157 1156 3034 Fragments arranged by size: 3034 2157 1156 1088 806 469 ApoI r'AATT_y Cuts at: 1493 2317 3049 5093 5296 6019 6245 6397 8506 Size: 824 732 2044 203 723 226 152 2109 Cuts at: 8506 1493 Size: 1697 Fragments arranged by size: 2109 2044 1697 824 732 723 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 7264 160 Size: 872 6232 1606 Fragments arranged by size: 6232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8710 AvaI C'yCGr_G Cuts at: 1870 1943 2991 3456 3842 4319 6035 1870 Size: 73 1048 465 386 477 1716 4545 Fragments arranged by size: 4545 1716 1048 477 465 386 73 BamHI G'GATC_C Cuts at: 6040 6407 6040 Size: 367 8343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 2863 4724 5276 5909 Size: 325 217 627 680 396 1861 552 633 Cuts at: 5909 6031 7486 618 Size: 122 1455 1842 Fragments arranged by size: 1861 1842 1455 680 633 627 552 396 325 217 122 BanII G_rGCy'C Cuts at: 1391 2356 3452 3974 4169 4634 4805 5349 5426 Size: 965 1096 522 195 465 171 544 77 Cuts at: 5426 5862 6029 1391 Size: 436 167 4072 Fragments arranged by size: 4072 1096 965 544 522 465 436 195 171 167 77 BbeI G_GCGC'C Cuts at: 5913 5913 Size: 8710 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2177 2240 2494 3856 928 Size: 971 278 63 254 1362 5782 Fragments arranged by size: 5782 1362 971 278 254 63 BbvCI CC'TCA_GC Cuts at: 4280 4280 Size: 8710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6901 6935 1903 Size: 34 4964 34 3678 Fragments arranged by size: 4964 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6602 7812 6602 Size: 1210 7500 BclI T'GATC_A Cuts at: 1548 1548 Size: 8710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2744 4523 4722 Size: 107 122 71 631 605 1072 1779 199 Cuts at: 4722 7321 136 Size: 2599 1525 Fragments arranged by size: 2599 1779 1525 1072 631 605 199 122 107 71 BglII A'GATC_T Cuts at: 8705 8705 Size: 8710 BlpI GC'TnA_GC Cuts at: 6127 6127 Size: 8710 Bme1580I G_kGCm'C Cuts at: 1790 2251 2356 2472 3339 4145 4614 4727 4805 Size: 461 105 116 867 806 469 113 78 Cuts at: 4805 6771 7927 1790 Size: 1966 1156 2573 Fragments arranged by size: 2573 1966 1156 867 806 469 461 116 113 105 78 BmgBI CAC'GTC Cuts at: 1318 2409 2556 4489 5434 1318 Size: 1091 147 1933 945 4594 Fragments arranged by size: 4594 1933 1091 945 147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4118 5164 5404 Size: 416 53 83 399 24 2701 1046 240 Cuts at: 5404 6441 7399 442 Size: 1037 958 1753 Fragments arranged by size: 2701 1753 1046 1037 958 416 399 240 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2170 2675 3428 3713 4077 4645 4909 4964 Size: 931 505 753 285 364 568 264 55 Cuts at: 4964 7370 1239 Size: 2406 2579 Fragments arranged by size: 2579 2406 931 753 568 505 364 285 264 55 Bpu10I CC'TnA_GC Cuts at: 2137 4280 5425 2137 Size: 2143 1145 5422 Fragments arranged by size: 5422 2143 1145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6835 7700 7998 6835 Size: 865 298 7547 Fragments arranged by size: 7547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 4486 5399 7373 882 Size: 617 2844 143 913 1974 2219 Fragments arranged by size: 2844 2219 1974 913 617 143 BsaAI yAC'GTr Cuts at: 493 1204 3123 3430 6501 493 Size: 711 1919 307 3071 2702 Fragments arranged by size: 3071 2702 1919 711 307 BsaBI GATnn'nnATC Cuts at: 8704 8704 Size: 8710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2871 2924 4643 5076 Size: 53 83 186 936 1338 53 1719 433 Cuts at: 5076 5910 6517 6899 7534 275 Size: 834 607 382 635 1451 Fragments arranged by size: 1719 1451 1338 936 834 635 607 433 382 186 83 53 53 BsaWI w'CCGG_w Cuts at: 3597 3981 5445 7143 7541 7815 7962 8373 3597 Size: 384 1464 1698 398 274 147 411 3934 Fragments arranged by size: 3934 1698 1464 411 398 384 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 4550 4580 4955 Size: 30 165 30 1173 30 1944 30 375 Cuts at: 4955 4961 4985 4991 5963 5993 1178 Size: 6 24 6 972 30 3895 Fragments arranged by size: 3895 1944 1173 972 375 165 30 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 3815 4343 4375 4555 5388 5405 1186 Size: 1261 1368 528 32 180 833 17 4491 Fragments arranged by size: 4491 1368 1261 833 528 180 32 17 BseYI C'CCAG_C Cuts at: 3951 4739 4908 5010 5378 7913 8409 3951 Size: 788 169 102 368 2535 496 4252 Fragments arranged by size: 4252 2535 788 496 368 169 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 3073 4024 4436 4889 5498 1163 Size: 573 653 684 951 412 453 609 4375 Fragments arranged by size: 4375 951 684 653 609 573 453 412 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4544 4721 6160 6921 7949 1540 Size: 275 186 2543 177 1439 761 1028 2301 Fragments arranged by size: 2543 2301 1439 1028 761 275 186 177 BsiHKAI G_wGCw'C Cuts at: 2251 2988 3339 3452 4145 4169 4614 4938 5426 Size: 737 351 113 693 24 445 324 488 Cuts at: 5426 5634 5862 6771 6856 7927 2251 Size: 208 228 909 85 1071 3034 Fragments arranged by size: 3034 1071 909 737 693 488 445 351 324 228 208 113 85 24 BsmI GAATG_Cn' Cuts at: 2264 3248 5612 6221 6314 2264 Size: 984 2364 609 93 4660 Fragments arranged by size: 4660 2364 984 609 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5424 5424 Size: 8710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2251 2356 2472 2988 3339 3452 3974 Size: 399 461 105 116 516 351 113 522 Cuts at: 3974 4145 4169 4614 4634 4727 4805 4938 5349 Size: 171 24 445 20 93 78 133 411 Cuts at: 5349 5426 5634 5862 6029 6771 6856 7927 1391 Size: 77 208 228 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 516 461 445 411 399 351 228 208 171 167 133 116 113 105 93 85 78 77 24 20 BspEI T'CCGG_A Cuts at: 3981 5445 8373 3981 Size: 1464 2928 4318 Fragments arranged by size: 4318 2928 1464 BspHI T'CATG_A Cuts at: 2094 3805 4917 5418 5616 6599 8329 2094 Size: 1711 1112 501 198 983 1730 2475 Fragments arranged by size: 2475 1730 1711 1112 983 501 198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2556 3307 6065 844 Size: 1712 751 2758 3489 Fragments arranged by size: 3489 2758 1712 751 BsrBI CCG'CTC Cuts at: 1394 1823 4966 5538 6597 1394 Size: 429 3143 572 1059 3507 Fragments arranged by size: 3507 3143 1059 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3678 7205 7379 65 Size: 1183 2430 3527 174 1396 Fragments arranged by size: 3527 2430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 4690 4716 5408 7354 7541 1700 Size: 110 1529 1351 26 692 1946 187 2869 Fragments arranged by size: 2869 1946 1529 1351 692 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8710 BssSI C'ACGA_G Cuts at: 3514 3530 5206 6770 7782 3514 Size: 16 1676 1564 1012 4442 Fragments arranged by size: 4442 1676 1564 1012 16 BstAPI GCAn_nnn'nTGC Cuts at: 794 3718 4038 794 Size: 2924 320 5466 Fragments arranged by size: 5466 2924 320 BstBI TT'CG_AA Cuts at: 3046 7514 3046 Size: 4468 4242 BstEII G'GTnAC_C Cuts at: 1184 5329 1184 Size: 4145 4565 BstXI CCAn_nnnn'nTGG Cuts at: 3274 3691 3946 4678 3274 Size: 417 255 732 7306 Fragments arranged by size: 7306 732 417 255 BstYI r'GATC_y Cuts at: 1972 2895 2946 2995 3495 3753 4923 4953 5448 Size: 923 51 49 500 258 1170 30 495 Cuts at: 5448 6040 6407 6792 6809 8261 8347 8359 8705 Size: 592 367 385 17 1452 86 12 346 Cuts at: 8705 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 923 592 500 495 385 367 346 258 86 51 49 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2461 3267 3975 4044 4863 5220 Size: 552 939 457 806 708 69 819 357 Cuts at: 5220 5955 513 Size: 735 3268 Fragments arranged by size: 3268 939 819 806 735 708 552 457 357 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2956 4498 5101 487 Size: 683 56 1730 1542 603 4096 Fragments arranged by size: 4096 1730 1542 683 603 56 BtsI GCAGTG_nn' Cuts at: 3204 3399 3510 5214 6226 7020 7040 3204 Size: 195 111 1704 1012 794 20 4874 Fragments arranged by size: 4874 1704 1012 794 195 111 20 ClaI AT'CG_AT Cuts at: 1554 2268 1554 Size: 714 7996 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8675 DraI TTT'AAA Cuts at: 6014 6014 Size: 8710 DraIII CAC_nnn'GTG Cuts at: 2467 3934 5112 5686 2467 Size: 1467 1178 574 5491 Fragments arranged by size: 5491 1467 1178 574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 7717 809 Size: 507 4202 2199 1802 Fragments arranged by size: 4202 2199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2458 2902 3467 Size: 54 1425 50 275 18 628 444 565 Cuts at: 3467 3544 3735 3936 4491 4541 4680 4714 4718 Size: 77 191 201 555 50 139 34 4 Cuts at: 4718 5236 5262 5677 5952 6157 7046 8675 8 Size: 518 26 415 275 205 889 1629 43 Fragments arranged by size: 1629 1425 889 628 565 555 518 444 415 275 275 205 201 191 139 77 54 50 50 43 34 26 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4541 4718 6157 1537 Size: 275 2729 177 1439 4090 Fragments arranged by size: 4090 2729 1439 275 177 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 3005 3017 4059 4926 6640 1353 Size: 684 968 12 1042 867 1714 3423 Fragments arranged by size: 3423 1714 1042 968 867 684 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3077 3539 3587 4849 5813 7297 Size: 1064 138 1488 462 48 1262 964 1484 Cuts at: 7297 7671 7817 387 Size: 374 146 1280 Fragments arranged by size: 1488 1484 1280 1262 1064 964 462 374 146 138 48 EcoICRI GAG'CTC Cuts at: 3450 4167 5424 5860 3450 Size: 717 1257 436 6300 Fragments arranged by size: 6300 1257 717 436 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2170 2675 2998 3428 3455 3650 3713 Size: 393 538 505 323 430 27 195 63 Cuts at: 3713 4077 4645 4909 4964 5738 6773 7370 8157 Size: 364 568 264 55 774 1035 597 787 Cuts at: 8157 8425 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 787 774 597 568 538 505 430 393 364 323 268 264 195 63 55 27 EcoNI CCTnn'n_nnAGG Cuts at: 2045 3533 2045 Size: 1488 7222 EcoO109I rG'GnC_Cy Cuts at: 2285 2352 3151 3383 4733 4801 2285 Size: 67 799 232 1350 68 6194 Fragments arranged by size: 6194 1350 799 232 68 67 EcoRI G'AATT_C Cuts at: 6019 6397 6019 Size: 378 8332 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3680 3680 Size: 8710 FseI GG_CCGG'CC Cuts at: 4720 4720 Size: 8710 FspI TGC'GCA Cuts at: 3856 5714 7216 3856 Size: 1858 1502 5350 Fragments arranged by size: 5350 1858 1502 FspAI rTGC'GCAy Cuts at: 5714 5714 Size: 8710 HaeII r_GCGC'y Cuts at: 1678 3804 4299 4896 5913 7857 1678 Size: 2126 495 597 1017 1944 2531 Fragments arranged by size: 2531 2126 1944 1017 597 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2257 2261 2272 Size: 32 135 32 574 32 79 4 11 Cuts at: 2272 2289 2293 2304 4550 4582 5847 5879 7351 Size: 17 4 11 2246 32 1265 32 1472 Cuts at: 7351 7383 7425 7457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2246 1472 1265 574 135 79 42 32 32 32 32 32 32 32 17 11 11 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2485 3163 3634 6054 6300 669 Size: 988 281 547 678 471 2420 246 3079 Fragments arranged by size: 3079 2420 988 678 547 471 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8710 HpaI GTT'AAC Cuts at: 6300 6300 Size: 8710 KasI G'GCGC_C Cuts at: 5909 5909 Size: 8710 KpnI G_GTAC'C Cuts at: 6035 6035 Size: 8710 MfeI C'AATT_G Cuts at: 6309 6309 Size: 8710 MluI A'CGCG_T Cuts at: 8586 8586 Size: 8710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3855 7824 8008 1305 Size: 2550 3969 184 2007 Fragments arranged by size: 3969 2550 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2460 3737 3938 4493 4682 Size: 54 1425 343 628 1277 201 555 189 Cuts at: 4682 5238 10 Size: 556 3482 Fragments arranged by size: 3482 1425 1277 628 556 555 343 201 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2454 3125 3272 3881 5219 5981 6668 Size: 648 1288 671 147 609 1338 762 687 Cuts at: 6668 7027 7186 518 Size: 359 159 2042 Fragments arranged by size: 2042 1338 1288 762 687 671 648 609 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2641 2731 3181 3241 3301 3590 3852 3860 Size: 1035 90 450 60 60 289 262 8 Cuts at: 3860 3955 4739 5505 5853 5902 5919 6126 6803 Size: 95 784 766 348 49 17 207 677 Cuts at: 6803 7951 8196 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1035 784 766 677 450 348 289 262 245 207 95 90 60 60 49 17 8 NaeI GCC'GGC Cuts at: 4718 4718 Size: 8710 NarI GG'CG_CC Cuts at: 5910 5910 Size: 8710 NcoI C'CATG_G Cuts at: 513 1065 2004 3267 3975 5220 513 Size: 552 939 1263 708 1245 4003 Fragments arranged by size: 4003 1263 1245 939 708 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8710 NgoMIV G'CCGG_C Cuts at: 4716 4716 Size: 8710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8710 NotI GC'GGCC_GC Cuts at: 6157 6157 Size: 8710 NspI r_CATG'y Cuts at: 1132 1229 2845 3130 3586 5359 5814 6068 7613 Size: 97 1616 285 456 1773 455 254 1545 Cuts at: 7613 1132 Size: 2229 Fragments arranged by size: 2229 1773 1616 1545 456 455 285 254 97 PasI CC'CwG_GG Cuts at: 1348 2929 3387 4309 1348 Size: 1581 458 922 5749 Fragments arranged by size: 5749 1581 922 458 PciI A'CATG_T Cuts at: 7609 7609 Size: 8710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2976 3733 3934 1108 Size: 1868 757 201 5884 Fragments arranged by size: 5884 1868 757 201 PfoI T'CCnGG_A Cuts at: 4554 4554 Size: 8710 PmeI GTTT'AAAC Cuts at: 6014 6014 Size: 8710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 4961 4993 6779 6811 8292 8324 3755 Size: 32 1174 32 1786 32 1481 32 4141 Fragments arranged by size: 4141 1786 1481 1174 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2285 3151 3383 2285 Size: 866 232 7612 Fragments arranged by size: 7612 866 232 PshAI GACnn'nnGTC Cuts at: 1613 3168 3381 1613 Size: 1555 213 6942 Fragments arranged by size: 6942 1555 213 PsiI TTA'TAA Cuts at: 6280 6280 Size: 8710 PspOMI G'GGCC_C Cuts at: 2352 4801 2352 Size: 2449 6261 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5186 5218 5186 Size: 32 8678 PstI C_TGCA'G Cuts at: 830 2553 3318 3898 4056 5664 6062 830 Size: 1723 765 580 158 1608 398 3478 Fragments arranged by size: 3478 1723 1608 765 580 398 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8710 PvuII CAG'CTG Cuts at: 2731 3181 3241 3301 3590 3955 5505 5919 2731 Size: 450 60 60 289 365 1550 414 5522 Fragments arranged by size: 5522 1550 450 414 365 289 60 60 SacI G_AGCT'C Cuts at: 3452 4169 5426 5862 3452 Size: 717 1257 436 6300 Fragments arranged by size: 6300 1257 717 436 SalI G'TCGA_C Cuts at: 1936 6052 1936 Size: 4116 4594 SanDI GG'GwC_CC Cuts at: 3383 3383 Size: 8710 SapI GCTCTTCn'nnn_ Cuts at: 3017 4926 3017 Size: 1909 6801 SbfI CC_TGCA'GG Cuts at: 3318 6062 3318 Size: 2744 5966 ScaI AGT'ACT Cuts at: 3554 4503 5879 6958 8670 3554 Size: 949 1376 1079 1712 3594 Fragments arranged by size: 3594 1712 1376 1079 949 SfcI C'TryA_G Cuts at: 826 1045 2549 2636 3314 3817 3894 4052 5121 Size: 219 1504 87 678 503 77 158 1069 Cuts at: 5121 5660 6058 7193 7874 8065 826 Size: 539 398 1135 681 191 1471 Fragments arranged by size: 1504 1471 1135 1069 681 678 539 503 398 219 191 158 87 77 SfoI GGC'GCC Cuts at: 5911 5911 Size: 8710 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8710 SmaI CCC'GGG Cuts at: 1872 2993 6037 1872 Size: 1121 3044 4545 Fragments arranged by size: 4545 3044 1121 SmlI C'TyrA_G Cuts at: 820 1017 1943 6814 7715 7977 820 Size: 197 926 4871 901 262 1553 Fragments arranged by size: 4871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6501 493 Size: 6008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8710 SphI G_CATG'C Cuts at: 1132 2845 6068 1132 Size: 1713 3223 3774 Fragments arranged by size: 3774 3223 1713 SspI AAT'ATT Cuts at: 5 52 5055 6634 5 Size: 47 5003 1579 2081 Fragments arranged by size: 5003 2081 1579 47 StuI AGG'CCT Cuts at: 1517 5654 1517 Size: 4137 4573 StyI C'CwwG_G Cuts at: 513 1065 2004 2348 2427 3267 3871 3975 5220 Size: 552 939 344 79 840 604 104 1245 Cuts at: 5220 6149 513 Size: 929 3074 Fragments arranged by size: 3074 1245 939 929 840 604 552 344 104 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2921 3553 3721 5632 6737 6754 6907 Size: 1255 1341 632 168 1911 1105 17 153 Cuts at: 6907 7092 7537 325 Size: 185 445 1498 Fragments arranged by size: 1911 1498 1341 1255 1105 632 445 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 3552 4501 Size: 274 80 33 51 1443 546 1029 949 Cuts at: 4501 4749 5877 5985 6956 8668 96 Size: 248 1128 108 971 1712 138 Fragments arranged by size: 1712 1443 1128 1029 971 949 546 274 248 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3500 3532 4955 4987 8670 8702 3500 Size: 32 1423 32 3683 32 3508 Fragments arranged by size: 3683 3508 1423 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8710 XbaI T'CTAG_A Cuts at: 6046 6046 Size: 8710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4306 5920 4306 Size: 1614 7096 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8710 XmaI C'CCGG_G Cuts at: 1870 2991 6035 1870 Size: 1121 3044 4545 Fragments arranged by size: 4545 3044 1121 XmnI GAAnn'nnTTC Cuts at: 4790 6839 4790 Size: 2049 6661 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6518 276 Size: 53 83 186 936 4984 2468 Fragments arranged by size: 4984 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV NruI NsiI PacI PmlI PspXI RsrII SacII SexAI SfiI SrfI SwaI