################################## # NO CUT ENZYMES ( 40 enzymes) # ################################## AatII AgeI AhdI Aor51HI AscI AspEI BbeI BbrPI BsiWI BssHII BstEII BstPI CpoI DrdI Eam1105I Eco47III EcoO65I FseI KasI MluI NarI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AclI 8237 AACGTT AA^CGTT AcyI 5123 GGCGTC G[GA]^CG[CT]C ApaI 4438 GGGCCC GGGCC^C AviII 5864 TGCGCA TGC^GCA AvrII 5305 CCTAGG C^CTAGG BlnI 5305 CCTAGG C^CTAGG BsaAI 7789 TACGTG [CT]AC^GT[GA] BsaHI 5123 GGCGTC G[GA]^CG[CT]C BsiEI 5035 CGGCCG CG[GA][CT]^CG BsmBI 5934 GAGACG CGTCTC(1/5) BsrBI 8433 GAGCGG CCGCTC(-3/-3) EagI 5035 CGGCCG C^GGCCG EclXI 5035 CGGCCG C^GGCCG Eco52I 5035 CGGCCG C^GGCCG FspI 5864 TGCGCA TGC^GCA Hin1I 5123 GGCGTC G[GA]^CG[CT]C HpaI 328 GTTAAC GTT^AAC KspI 64 CCGCGG CCGC^GG NaeI 5037 GCCGGC GCC^GGC NgoMIV 5037 GCCGGC G^CCGGC NheI 9747 GCTAGC G^CTAGC Psp1406I 8237 AACGTT AA^CGTT SacII 64 CCGCGG CCGC^GG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccII 65 CGCG CG^CG 3083 CGCG AccIII 3837 TCCGGA T^CCGGA 10625 TCCGGA AflII 2152 CTTAAG C^TTAAG 8730 CTTAAG Alw44I 1945 GTGCAC G^TGCAC 2419 GTGCAC AocI 1756 CCTGAGG CC^T[ACGT]AGG 3538 CCTGAGG ApaLI 1945 GTGCAC G^TGCAC 2419 GTGCAC BclI 2025 TGATCA T^GATCA 2871 TGATCA BfrI 2152 CTTAAG C^TTAAG 8730 CTTAAG BglI 3592 GCCGATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4867 GCCAGCTGGGC BsaWI 3837 TCCGGA [AT]^CCGG[AT] 10625 TCCGGA BseAI 3837 TCCGGA T^CCGGA 10625 TCCGGA BspDI 417 ATCGAT AT^CGAT 8779 ATCGAT BspEI 3837 TCCGGA T^CCGGA 10625 TCCGGA BspLU11I 3827 ACATGT A^CATGT 5921 ACATGT BsrFI 5037 GCCGGC [GA]^CCGG[CT] 9038 ACCGGC Bst1107I 4194 GTATAC GTA^TAC 8550 GTATAC BstAPI 4285 GCAAAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 9790 GCAGGTGGTGC BstUI 65 CGCG CG^CG 3083 CGCG BstXI 1467 CCAGACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3215 CCATTATGATGG BstZ17I 4194 GTATAC GTA^TAC 8550 GTATAC Bsu36I 1756 CCTGAGG CC^T[ACGT]AGG 3538 CCTGAGG Cfr10I 5037 GCCGGC [GA]^CCGG[CT] 9038 ACCGGC ClaI 417 ATCGAT AT^CGAT 8779 ATCGAT Eco81I 1756 CCTGAGG CC^T[ACGT]AGG 3538 CCTGAGG EcoNI 2166 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3186 CCTATCTCAGG EcoRI 5939 GAATTC G^AATTC 8571 GAATTC EcoRV 5133 GATATC GAT^ATC 8497 GATATC EcoT22I 2987 ATGCAT ATGCA^T 3008 ATGCAT FbaI 2025 TGATCA T^GATCA 2871 TGATCA HaeII 1896 GGCGCT [GA]GCGC^[CT] 7889 GGCGCT MroI 3837 TCCGGA T^CCGGA 10625 TCCGGA MvnI 65 CGCG CG^CG 3083 CGCG NsiI 2987 ATGCAT ATGCA^T 3008 ATGCAT PpuMI 1118 AGGACCT [GA]G^G[AT]CC[CT] 1260 GGGTCCT PshAI 7771 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 10075 GACACTGGTC SexAI 1331 ACCAGGT A^CC[AT]GGT 8477 ACCTGGT SmaI 15 CCCGGG CCC^GGG 470 CCCGGG SpeI 552 ACTAGT A^CTAGT 2415 ACTAGT SphI 2335 GCATGC GCATG^C 5394 GCATGC XmaCI 15 CCCGGG C^CCGGG 470 CCCGGG XmaI 15 CCCGGG C^CCGGG 470 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 154 enzymes) # ######################################### Acc65I AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BssSI BstBI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI NspV PflFI PflMI PleI PshBI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfuI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoII XmnI