(Circular) (Six-base) MAPSORT of: pFN21AB9894.seq Check: 9288 from: 1 to: 15688 pFN21AB9894 15688 bp With 182 enzymes: SgfI * December 22, 2010 15:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 10460 11038 11786 10460 Size: 578 748 14362 Fragments arranged by size: 14362 748 578 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 13498 278 Size: 53 83 186 936 11962 2468 Fragments arranged by size: 11962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3751 3816 4351 6200 10556 11349 12155 13031 Size: 1814 65 535 1849 4356 793 806 876 Cuts at: 13031 1937 Size: 4594 Fragments arranged by size: 4594 4356 1849 1814 876 806 793 535 65 Acc65I G'GTAC_C Cuts at: 3482 7592 11114 13009 3482 Size: 4110 3522 1895 6161 Fragments arranged by size: 6161 4110 3522 1895 AclI AA'CG_TT Cuts at: 1563 10243 13815 14188 1563 Size: 8680 3572 373 3063 Fragments arranged by size: 8680 3572 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3235 4742 5283 5367 5843 6121 6813 Size: 977 626 1507 541 84 476 278 692 Cuts at: 6813 6864 6978 7058 7203 7524 7608 8189 9591 Size: 51 114 80 145 321 84 581 1402 Cuts at: 9591 10490 10550 10709 11086 13751 15135 15403 1632 Size: 899 60 159 377 2665 1384 268 1917 Fragments arranged by size: 2665 1917 1507 1402 1384 977 899 692 626 581 541 476 377 321 278 268 159 145 114 84 84 80 60 51 AflII C'TTAA_G Cuts at: 820 1017 4157 10735 820 Size: 197 3140 6578 5773 Fragments arranged by size: 6578 5773 3140 197 AflIII A'CryG_T Cuts at: 1203 5832 7926 10638 12457 14587 15564 1203 Size: 4629 2094 2712 1819 2130 977 1327 Fragments arranged by size: 4629 2712 2130 2094 1819 1327 977 AgeI A'CCGG_T Cuts at: 14519 14519 Size: 15688 AhdI GACnn_n'nnGTC Cuts at: 14417 14417 Size: 15688 AleI CACnn'nnGTG Cuts at: 1166 5391 1166 Size: 4225 11463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5596 8970 9951 1184 Size: 4412 3374 981 6921 Fragments arranged by size: 6921 4412 3374 981 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4646 4678 6231 6263 6915 6947 9039 9071 11424 Size: 32 1553 32 652 32 2092 32 2353 Cuts at: 11424 11456 4646 Size: 32 8878 Fragments arranged by size: 8878 2353 2092 1553 652 32 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3097 4681 4733 4963 7423 10468 11109 12047 12223 Size: 1584 52 230 2460 3045 641 938 176 Cuts at: 12223 12746 12905 13431 15003 3097 Size: 523 159 526 1572 3782 Fragments arranged by size: 3782 3045 2460 1584 1572 938 641 526 523 230 176 159 52 ApaI G_GGCC'C Cuts at: 6447 6447 Size: 15688 ApaLI G'TGCA_C Cuts at: 3950 4424 13745 14901 3950 Size: 474 9321 1156 4737 Fragments arranged by size: 9321 4737 1156 474 ApoI r'AATT_y Cuts at: 1493 2188 2525 2720 3508 3827 4280 4515 4524 Size: 695 337 195 788 319 453 235 9 Cuts at: 4524 5726 6293 7287 7634 7944 9680 10576 10582 Size: 1202 567 994 347 310 1736 896 6 Cuts at: 10582 10962 12997 13223 13375 15484 1493 Size: 380 2035 226 152 2109 1697 Fragments arranged by size: 2109 2035 1736 1697 1202 994 896 788 695 567 453 380 347 337 319 310 235 226 195 152 9 6 AseI AT'TA_AT Cuts at: 160 1032 4982 8544 10823 14242 160 Size: 872 3950 3562 2279 3419 1606 Fragments arranged by size: 3950 3562 3419 2279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 15688 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2475 10398 13013 1870 Size: 73 77 455 7923 2615 4545 Fragments arranged by size: 7923 4545 2615 455 77 73 AvrII C'CTAG_G Cuts at: 7310 7310 Size: 15688 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2214 2247 6800 6833 2214 Size: 33 4553 33 11069 Fragments arranged by size: 11069 4553 33 33 BamHI G'GATC_C Cuts at: 3406 5845 6597 13018 13385 3406 Size: 2439 752 6421 367 5709 Fragments arranged by size: 6421 5709 2439 752 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 3482 6838 7592 10486 Size: 325 217 627 270 1425 3356 754 2894 Cuts at: 10486 11114 11384 11801 13009 14464 618 Size: 628 270 417 1208 1455 1842 Fragments arranged by size: 3356 2894 1842 1455 1425 1208 754 628 627 417 325 270 270 217 BanII G_rGCy'C Cuts at: 1391 2020 4813 6447 6652 7899 8904 10017 10053 Size: 629 2793 1634 205 1247 1005 1113 36 Cuts at: 10053 13007 1391 Size: 2954 4072 Fragments arranged by size: 4072 2954 2793 1634 1247 1113 1005 629 205 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 3108 4730 6819 7098 7773 8177 Size: 971 588 621 1622 2089 279 675 404 Cuts at: 8177 10004 11235 12656 928 Size: 1827 1231 1421 3960 Fragments arranged by size: 3960 2089 1827 1622 1421 1231 971 675 621 588 404 279 BbvCI CC'TCA_GC Cuts at: 2327 3120 3128 2327 Size: 793 8 14887 Fragments arranged by size: 14887 793 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2360 2394 4988 5022 5588 5622 13879 Size: 34 423 34 2594 34 566 34 8257 Cuts at: 13879 13913 1903 Size: 34 3678 Fragments arranged by size: 8257 3678 2594 566 423 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2076 2860 2947 6756 7587 8981 9966 13580 14790 Size: 784 87 3809 831 1394 985 3614 1210 Cuts at: 14790 2076 Size: 2974 Fragments arranged by size: 3809 3614 2974 1394 1210 985 831 784 87 BclI T'GATC_A Cuts at: 1548 4030 4876 1548 Size: 2482 846 12360 Fragments arranged by size: 12360 2482 846 BfrBI ATG'CAT Cuts at: 4994 5015 4994 Size: 21 15667 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5603 6878 14299 Size: 107 122 71 631 605 3931 1275 7421 Cuts at: 14299 136 Size: 1525 Fragments arranged by size: 7421 3931 1525 1275 631 605 122 107 71 BglII A'GATC_T Cuts at: 2697 8960 11620 15683 2697 Size: 6263 2660 4063 2702 Fragments arranged by size: 6263 4063 2702 2660 BlpI GC'TnA_GC Cuts at: 2033 3662 6651 8801 12957 13105 2033 Size: 1629 2989 2150 4156 148 4616 Fragments arranged by size: 4616 4156 2989 2150 1629 148 Bme1580I G_kGCm'C Cuts at: 1790 3730 3856 3954 4428 6447 13749 14905 1790 Size: 1940 126 98 474 2019 7302 1156 2573 Fragments arranged by size: 7302 2573 2019 1940 1156 474 126 98 BmgBI CAC'GTC Cuts at: 1318 2599 6172 1318 Size: 1281 3573 10834 Fragments arranged by size: 10834 3573 1281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4811 9161 12807 Size: 416 53 83 399 24 3394 4350 3646 Cuts at: 12807 13419 14377 442 Size: 612 958 1753 Fragments arranged by size: 4350 3646 3394 1753 958 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 11756 1056 Size: 10700 4988 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 12838 12838 Size: 15688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3482 4994 5407 5808 7445 8495 9066 9183 Size: 2243 1512 413 401 1637 1050 571 117 Cuts at: 9183 9266 9563 9881 9938 10392 12455 14348 1239 Size: 83 297 318 57 454 2063 1893 2579 Fragments arranged by size: 2579 2243 2063 1893 1637 1512 1050 571 454 413 401 318 297 117 83 57 Bpu10I CC'TnA_GC Cuts at: 2327 3120 3128 4493 5449 9491 11711 12303 2327 Size: 793 8 1365 956 4042 2220 592 5712 Fragments arranged by size: 5712 4042 2220 1365 956 793 592 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4797 7536 10896 10998 11346 11771 13813 14678 14976 Size: 2739 3360 102 348 425 2042 865 298 Cuts at: 14976 4797 Size: 5509 Fragments arranged by size: 5509 3360 2739 2042 865 425 348 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2031 2484 9221 12780 14351 882 Size: 617 532 453 6737 3559 1571 2219 Fragments arranged by size: 6737 3559 2219 1571 617 532 453 BsaAI yAC'GTr Cuts at: 493 1204 9796 13479 493 Size: 711 8592 3683 2702 Fragments arranged by size: 8592 3683 2702 711 BsaBI GATnn'nnATC Cuts at: 8484 10988 11037 12879 15682 8484 Size: 2504 49 1842 2803 8490 Fragments arranged by size: 8490 2803 2504 1842 49 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7129 13495 13877 14512 Size: 53 83 186 936 5596 6366 382 635 Cuts at: 14512 275 Size: 1451 Fragments arranged by size: 6366 5596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5842 12630 14121 14519 14793 14940 15351 5842 Size: 6788 1491 398 274 147 411 6179 Fragments arranged by size: 6788 6179 1491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4701 4731 5011 5041 6915 Size: 30 165 30 3298 30 280 30 1874 Cuts at: 6915 6945 8077 8107 9039 9069 1178 Size: 30 1132 30 932 30 7797 Fragments arranged by size: 7797 3298 1874 1132 932 280 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2744 3162 3247 8092 8712 1186 Size: 1558 418 85 4845 620 8162 Fragments arranged by size: 8162 4845 1558 620 418 85 BseYI C'CCAG_C Cuts at: 5459 6361 6876 7903 9296 10083 14891 15387 5459 Size: 902 515 1027 1393 787 4808 496 5760 Fragments arranged by size: 5760 4808 1393 1027 902 787 515 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3186 4749 5633 6363 6367 7437 10377 Size: 573 1450 1563 884 730 4 1070 2940 Cuts at: 10377 11344 11742 1163 Size: 967 398 5109 Fragments arranged by size: 5109 2940 1563 1450 1070 967 884 730 573 398 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7043 13138 13899 14927 1540 Size: 275 186 5042 6095 761 1028 2301 Fragments arranged by size: 6095 5042 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3663 3954 3999 4428 4813 5445 6652 7346 7431 Size: 291 45 429 385 632 1207 694 85 Cuts at: 7431 7887 7899 8251 8904 9621 9880 9906 10017 Size: 456 12 352 653 717 259 26 111 Cuts at: 10017 10053 11341 12249 13749 13834 14905 3663 Size: 36 1288 908 1500 85 1071 4446 Fragments arranged by size: 4446 1500 1288 1207 1071 908 717 694 653 632 456 429 385 352 291 259 111 85 85 45 36 26 12 BsmI GAATG_Cn' Cuts at: 2901 9713 9826 10892 11255 12682 13199 13292 2901 Size: 6812 113 1066 363 1427 517 93 5297 Fragments arranged by size: 6812 5297 1427 1066 517 363 113 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7933 7933 Size: 15688 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 3663 3730 3856 3954 3999 4428 Size: 399 230 1643 67 126 98 45 429 Cuts at: 4428 4813 5445 6447 6652 7346 7431 7887 7899 Size: 385 632 1002 205 694 85 456 12 Cuts at: 7899 8251 8904 9621 9880 9906 10017 10053 11341 Size: 352 653 717 259 26 111 36 1288 Cuts at: 11341 12249 13007 13749 13834 14905 1391 Size: 908 758 742 85 1071 2174 Fragments arranged by size: 2174 1643 1288 1071 1002 908 758 742 717 694 653 632 456 429 399 385 352 259 230 205 126 111 98 85 85 67 45 36 26 12 BspEI T'CCGG_A Cuts at: 5842 12630 15351 5842 Size: 6788 2721 6179 Fragments arranged by size: 6788 6179 2721 BspHI T'CATG_A Cuts at: 5106 8019 8110 10264 13577 15307 5106 Size: 2913 91 2154 3313 1730 5487 Fragments arranged by size: 5487 3313 2913 2154 1730 91 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3007 9187 10460 11038 11786 13043 844 Size: 2163 6180 1273 578 748 1257 3489 Fragments arranged by size: 6180 3489 2163 1273 1257 748 578 BsrBI CCG'CTC Cuts at: 1394 1823 10440 13575 1394 Size: 429 8617 3135 3507 Fragments arranged by size: 8617 3507 3135 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6510 7370 11355 14183 14357 65 Size: 1183 5262 860 3985 2828 174 1396 Fragments arranged by size: 5262 3985 2828 1396 1183 860 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7042 11043 14332 14519 1700 Size: 110 5232 4001 3289 187 2869 Fragments arranged by size: 5232 4001 3289 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2590 3303 7233 11179 96 Size: 2494 713 3930 3946 4605 Fragments arranged by size: 4605 3946 3930 2494 713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 15688 BssSI C'ACGA_G Cuts at: 4183 7391 7856 13748 14760 4183 Size: 3208 465 5892 1012 5111 Fragments arranged by size: 5892 5111 3208 1012 465 BstAPI GCAn_nnn'nTGC Cuts at: 794 6296 11801 794 Size: 5502 5505 4681 Fragments arranged by size: 5505 5502 4681 BstBI TT'CG_AA Cuts at: 4846 9124 10580 12445 14492 4846 Size: 4278 1456 1865 2047 6042 Fragments arranged by size: 6042 4278 2047 1865 1456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 15688 BstXI CCAn_nnnn'nTGG Cuts at: 3479 5227 3479 Size: 1748 13940 BstYI r'GATC_y Cuts at: 1972 2282 2697 3406 5845 6597 8960 10283 11620 Size: 310 415 709 2439 752 2363 1323 1337 Cuts at: 11620 13018 13385 13770 13787 15239 15325 15337 15683 Size: 1398 367 385 17 1452 86 12 346 Cuts at: 15683 1972 Size: 1977 Fragments arranged by size: 2439 2363 1977 1452 1398 1337 1323 752 709 415 385 367 346 310 86 17 12 BstZ17I GTA'TAC Cuts at: 6201 10557 6201 Size: 4356 11332 Bsu36I CC'TnA_GG Cuts at: 3762 5544 3762 Size: 1782 13906 BtgI C'CryG_G Cuts at: 513 1065 2069 4921 6179 7151 7457 7833 8294 Size: 552 1004 2852 1258 972 306 376 461 Cuts at: 8294 9907 9942 10515 11666 513 Size: 1613 35 573 1151 4535 Fragments arranged by size: 4535 2852 1613 1258 1151 1004 972 573 552 461 376 306 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5977 12013 487 Size: 683 56 4751 6036 4162 Fragments arranged by size: 6036 4751 4162 683 56 BtsI GCAGTG_nn' Cuts at: 3663 4751 5145 6355 6375 7255 7449 7641 10113 Size: 1088 394 1210 20 880 194 192 2472 Cuts at: 10113 10481 11505 12837 13204 13998 14018 3663 Size: 368 1024 1332 367 794 20 5333 Fragments arranged by size: 5333 2472 1332 1210 1088 1024 880 794 394 368 367 194 192 20 20 ClaI AT'CG_AT Cuts at: 1554 2423 10785 1554 Size: 869 8362 6457 Fragments arranged by size: 8362 6457 869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 15653 DraI TTT'AAA Cuts at: 2130 5916 6403 8218 12992 2130 Size: 3786 487 1815 4774 4826 Fragments arranged by size: 4826 4774 3786 1815 487 DraIII CAC_nnn'GTG Cuts at: 7333 9155 10486 11322 7333 Size: 1822 1331 836 11699 Fragments arranged by size: 11699 1822 1331 836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 14695 809 Size: 507 13379 1802 Fragments arranged by size: 13379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6870 7040 7365 Size: 54 1425 50 275 18 5040 170 325 Cuts at: 7365 9328 10873 11238 12643 13135 14024 15653 8 Size: 1963 1545 365 1405 492 889 1629 43 Fragments arranged by size: 5040 1963 1629 1545 1425 1405 889 492 365 325 275 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7040 13135 1537 Size: 275 5228 6095 4090 Fragments arranged by size: 6095 5228 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3087 4269 4372 5542 6140 6735 6990 7964 Size: 1734 1182 103 1170 598 595 255 974 Cuts at: 7964 7998 8334 8441 8838 8853 10005 10728 11052 Size: 34 336 107 397 15 1152 723 324 Cuts at: 11052 12839 13618 1353 Size: 1787 779 3423 Fragments arranged by size: 3423 1787 1734 1182 1170 1152 974 779 723 598 595 397 336 324 255 107 103 34 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8394 14275 14649 14795 387 Size: 1064 138 6805 5881 374 146 1280 Fragments arranged by size: 6805 5881 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4811 6650 7897 8902 10015 10051 4811 Size: 1839 1247 1005 1113 36 10448 Fragments arranged by size: 10448 1839 1247 1113 1005 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 3235 3482 4742 4994 5283 5367 Size: 393 977 626 247 1260 252 289 84 Cuts at: 5367 5407 5808 5843 6121 6813 6864 6978 7058 Size: 40 401 35 278 692 51 114 80 Cuts at: 7058 7203 7445 7524 7608 8189 8495 9066 9183 Size: 145 242 79 84 581 306 571 117 Cuts at: 9183 9266 9563 9591 9881 9938 10392 10490 10550 Size: 83 297 28 290 57 454 98 60 Cuts at: 10550 10709 11086 12455 13751 14348 15135 15403 1239 Size: 159 377 1369 1296 597 787 268 1524 Fragments arranged by size: 1524 1369 1296 1260 977 787 692 626 597 581 571 454 401 393 377 306 297 290 289 278 268 252 247 242 159 145 117 114 98 84 84 83 80 79 60 57 51 40 35 28 EcoNI CCTnn'n_nnAGG Cuts at: 4175 5195 4175 Size: 1020 14668 EcoO109I rG'GnC_Cy Cuts at: 3124 3266 6443 3124 Size: 142 3177 12369 Fragments arranged by size: 12369 3177 142 EcoRI G'AATT_C Cuts at: 7944 10576 12997 13375 7944 Size: 2632 2421 378 10257 Fragments arranged by size: 10257 2632 2421 378 EcoRV GAT'ATC Cuts at: 7140 10504 7140 Size: 3364 12324 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 4187 10277 11116 11542 11642 2824 Size: 1363 6090 839 426 100 6870 Fragments arranged by size: 6870 6090 1363 839 426 100 FspI TGC'GCA Cuts at: 7871 14194 7871 Size: 6323 9365 HaeII r_GCGC'y Cuts at: 1678 3905 9898 14835 1678 Size: 2227 5993 4937 2531 Fragments arranged by size: 5993 4937 2531 2227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 3922 3954 5088 Size: 32 135 32 521 32 1797 32 1134 Cuts at: 5088 5120 5757 5789 6749 6781 7799 7831 8343 Size: 32 637 32 960 32 1018 32 512 Cuts at: 8343 8375 8891 8923 12653 12685 14329 14361 14403 Size: 32 516 32 3730 32 1644 32 42 Cuts at: 14403 14435 1373 Size: 32 2626 Fragments arranged by size: 3730 2626 1797 1644 1134 1018 960 637 521 516 512 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3363 4701 6495 6907 12141 Size: 988 281 397 1028 1338 1794 412 5234 Cuts at: 12141 12916 13032 13278 669 Size: 775 116 246 3079 Fragments arranged by size: 5234 3079 1794 1338 1028 988 775 412 397 281 246 116 HindIII A'AGCT_T Cuts at: 748 6144 9493 9745 748 Size: 5396 3349 252 6691 Fragments arranged by size: 6691 5396 3349 252 HpaI GTT'AAC Cuts at: 2335 13278 2335 Size: 10943 4745 KpnI G_GTAC'C Cuts at: 3486 7596 11118 13013 3486 Size: 4110 3522 1895 6161 Fragments arranged by size: 6161 4110 3522 1895 MfeI C'AATT_G Cuts at: 2567 3864 4645 4830 5592 5879 13287 2567 Size: 1297 781 185 762 287 7408 4968 Fragments arranged by size: 7408 4968 1297 781 762 287 185 MluI A'CGCG_T Cuts at: 15564 15564 Size: 15688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2285 2953 3935 4632 5284 6204 6418 6766 Size: 980 668 982 697 652 920 214 348 Cuts at: 6766 7676 8965 9447 10852 12611 12953 14802 14986 Size: 910 1289 482 1405 1759 342 1849 184 Cuts at: 14986 1305 Size: 2007 Fragments arranged by size: 2007 1849 1759 1405 1289 982 980 920 910 697 668 652 482 348 342 214 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6872 7367 9330 10875 11240 Size: 54 1425 343 5040 495 1963 1545 365 Cuts at: 11240 12645 10 Size: 1405 3053 Fragments arranged by size: 5040 3053 1963 1545 1425 1405 495 365 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5225 5391 5578 7358 7404 7929 8490 Size: 648 4059 166 187 1780 46 525 561 Cuts at: 8490 8978 9770 9933 10124 10269 10615 13646 14005 Size: 488 792 163 191 145 346 3031 359 Cuts at: 14005 14164 518 Size: 159 2042 Fragments arranged by size: 4059 3031 2042 1780 792 648 561 525 488 359 346 191 187 166 163 159 145 46 MspA1I CmG'CkG Cuts at: 1606 2040 2071 2195 3368 4220 6064 6361 6631 Size: 434 31 124 1173 852 1844 297 270 Cuts at: 6631 6876 8090 8205 9859 10381 10871 11264 11464 Size: 245 1214 115 1654 522 490 393 200 Cuts at: 11464 11578 11587 12716 12961 13104 13781 14929 15174 Size: 114 9 1129 245 143 677 1148 245 Cuts at: 15174 1606 Size: 2120 Fragments arranged by size: 2120 1844 1654 1214 1173 1148 1129 852 677 522 490 434 393 297 270 245 245 245 200 143 124 115 114 31 9 NaeI GCC'GGC Cuts at: 7044 7044 Size: 15688 NcoI C'CATG_G Cuts at: 513 1065 4921 6179 7151 7457 7833 8294 10515 Size: 552 3856 1258 972 306 376 461 2221 Cuts at: 10515 513 Size: 5686 Fragments arranged by size: 5686 3856 2221 1258 972 552 461 376 306 NdeI CA'TA_TG Cuts at: 387 3115 3286 3445 4754 387 Size: 2728 171 159 1309 11321 Fragments arranged by size: 11321 2728 1309 171 159 NgoMIV G'CCGG_C Cuts at: 7042 7042 Size: 15688 NheI G'CTAG_C Cuts at: 1052 11752 1052 Size: 10700 4988 NotI GC'GGCC_GC Cuts at: 13135 13135 Size: 15688 NsiI A_TGCA'T Cuts at: 4996 5017 4996 Size: 21 15667 NspI r_CATG'y Cuts at: 1132 1229 4344 4358 5577 5836 6503 7403 7930 Size: 97 3115 14 1219 259 667 900 527 Cuts at: 7930 8075 10775 10808 10906 13046 14591 1132 Size: 145 2700 33 98 2140 1545 2229 Fragments arranged by size: 3115 2700 2229 2140 1545 1219 900 667 527 259 145 98 97 33 14 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 15688 PciI A'CATG_T Cuts at: 5832 7926 14587 5832 Size: 2094 6661 6933 Fragments arranged by size: 6933 6661 2094 PflMI CCAn_nnn'nTGG Cuts at: 1108 4681 6268 6858 8374 10974 12433 12746 1108 Size: 3573 1587 590 1516 2600 1459 313 4050 Fragments arranged by size: 4050 3573 2600 1587 1516 1459 590 313 PfoI T'CCnGG_A Cuts at: 8731 12471 12739 8731 Size: 3740 268 11680 Fragments arranged by size: 11680 3740 268 PmeI GTTT'AAAC Cuts at: 12992 12992 Size: 15688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6915 6947 9039 9071 10825 10857 11769 11801 13757 Size: 32 2092 32 1754 32 912 32 1956 Cuts at: 13757 13789 15270 15302 6915 Size: 32 1481 32 7301 Fragments arranged by size: 7301 2092 1956 1754 1481 912 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3124 3266 3124 Size: 142 15546 PshAI GACnn'nnGTC Cuts at: 1613 9780 12084 1613 Size: 8167 2304 5217 Fragments arranged by size: 8167 5217 2304 PsiI TTA'TAA Cuts at: 5552 13258 5552 Size: 7706 7982 PspOMI G'GGCC_C Cuts at: 6443 6443 Size: 15688 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3282 3314 4039 4071 7830 7862 3282 Size: 32 725 32 3759 32 11108 Fragments arranged by size: 11108 3759 725 32 32 32 PstI C_TGCA'G Cuts at: 830 2847 4338 4906 4959 5140 8101 8971 9531 Size: 2017 1491 568 53 181 2961 870 560 Cuts at: 9531 9819 9888 9954 10137 10433 10707 11577 11915 Size: 288 69 66 183 296 274 870 338 Cuts at: 11915 11956 12625 13040 830 Size: 41 669 415 3478 Fragments arranged by size: 3478 2961 2017 1491 870 870 669 568 560 415 338 296 288 274 183 181 69 66 53 41 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 15688 PvuII CAG'CTG Cuts at: 2195 3368 4220 6064 6361 6631 6876 8205 9859 Size: 1173 852 1844 297 270 245 1329 1654 Cuts at: 9859 10381 10871 11264 11464 11587 12716 12961 2195 Size: 522 490 393 200 123 1129 245 4922 Fragments arranged by size: 4922 1844 1654 1329 1173 1129 852 522 490 393 297 270 245 245 200 123 SacI G_AGCT'C Cuts at: 4813 6652 7899 8904 10017 10053 4813 Size: 1839 1247 1005 1113 36 10448 Fragments arranged by size: 10448 1839 1247 1113 1005 36 SacII CC_GC'GG Cuts at: 2072 2072 Size: 15688 SalI G'TCGA_C Cuts at: 1936 13030 1936 Size: 11094 4594 SapI GCTCTTCn'nnn_ Cuts at: 8334 8441 10005 8334 Size: 107 1564 14017 Fragments arranged by size: 14017 1564 107 SbfI CC_TGCA'GG Cuts at: 13040 13040 Size: 15688 ScaI AGT'ACT Cuts at: 3223 5972 11011 13936 15648 3223 Size: 2749 5039 2925 1712 3263 Fragments arranged by size: 5039 3263 2925 2749 1712 SexAI A'CCwGG_T Cuts at: 3336 10482 3336 Size: 7146 8542 SfcI C'TryA_G Cuts at: 826 1045 2266 2790 2843 3633 4334 4666 4902 Size: 219 1221 524 53 790 701 332 236 Cuts at: 4902 4955 5136 6768 8097 8967 9527 9629 9815 Size: 53 181 1632 1329 870 560 102 186 Cuts at: 9815 9884 9950 10054 10133 10429 10703 11081 11573 Size: 69 66 104 79 296 274 378 492 Cuts at: 11573 11911 11952 12621 13036 14171 14852 15043 826 Size: 338 41 669 415 1135 681 191 1471 Fragments arranged by size: 1632 1471 1329 1221 1135 870 790 701 681 669 560 524 492 415 378 338 332 296 274 236 219 191 186 181 104 102 79 69 66 53 53 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 15688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 15688 SmaI CCC'GGG Cuts at: 1872 2022 2477 13015 1872 Size: 150 455 10538 4545 Fragments arranged by size: 10538 4545 455 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 4157 4812 7551 10735 10911 11013 Size: 197 926 2214 655 2739 3184 176 102 Cuts at: 11013 11361 11786 13792 14693 14955 820 Size: 348 425 2006 901 262 1553 Fragments arranged by size: 3184 2739 2214 2006 1553 926 901 655 425 348 262 197 176 102 SnaBI TAC'GTA Cuts at: 493 13479 493 Size: 12986 2702 SpeI A'CTAG_T Cuts at: 152 2557 4420 152 Size: 2405 1863 11420 Fragments arranged by size: 11420 2405 1863 SphI G_CATG'C Cuts at: 1132 4344 7403 13046 1132 Size: 3212 3059 5643 3774 Fragments arranged by size: 5643 3774 3212 3059 SspI AAT'ATT Cuts at: 5 52 2135 3676 3708 4152 7224 7669 13612 Size: 47 2083 1541 32 444 3072 445 5943 Cuts at: 13612 5 Size: 2081 Fragments arranged by size: 5943 3072 2083 2081 1541 445 444 47 32 StuI AGG'CCT Cuts at: 1517 3767 4293 5366 9251 10730 1517 Size: 2250 526 1073 3885 1479 6475 Fragments arranged by size: 6475 3885 2250 1479 1073 526 StyI C'CwwG_G Cuts at: 513 1065 4921 5031 6179 6418 7151 7310 7457 Size: 552 3856 110 1148 239 733 159 147 Cuts at: 7457 7833 8294 9001 9778 10515 13127 513 Size: 376 461 707 777 737 2612 3074 Fragments arranged by size: 3856 3074 2612 1148 777 737 733 707 552 461 376 239 159 147 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2508 3015 7462 9143 9638 10873 11333 Size: 1255 928 507 4447 1681 495 1235 460 Cuts at: 11333 12362 12973 13715 13732 13885 14070 14515 325 Size: 1029 611 742 17 153 185 445 1498 Fragments arranged by size: 4447 1681 1498 1255 1235 1029 928 742 611 507 495 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2590 3221 3303 Size: 274 80 33 51 1443 613 631 82 Cuts at: 3303 5970 6832 7233 11009 11179 11824 13934 15646 Size: 2667 862 401 3776 170 645 2110 1712 Cuts at: 15646 96 Size: 138 Fragments arranged by size: 3776 2667 2110 1712 1443 862 645 631 613 401 274 170 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2050 2082 4701 4733 5375 5407 6745 6777 7017 Size: 32 2619 32 642 32 1338 32 240 Cuts at: 7017 7049 7633 7665 8077 8109 8550 8582 15648 Size: 32 584 32 412 32 441 32 7066 Cuts at: 15648 15680 2050 Size: 32 2058 Fragments arranged by size: 7066 2619 2058 1338 642 584 441 412 240 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3434 8357 12920 1316 Size: 2118 4923 4563 4084 Fragments arranged by size: 4923 4563 4084 2118 XbaI T'CTAG_A Cuts at: 9398 9559 12943 13024 9398 Size: 161 3384 81 12062 Fragments arranged by size: 12062 3384 161 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3140 5227 8627 10835 11812 3140 Size: 2087 3400 2208 977 7016 Fragments arranged by size: 7016 3400 2208 2087 977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 15688 XmaI C'CCGG_G Cuts at: 1870 2020 2475 13013 1870 Size: 150 455 10538 4545 Fragments arranged by size: 10538 4545 455 150 XmnI GAAnn'nnTTC Cuts at: 2529 2699 4121 6930 9054 10611 10722 10840 12213 Size: 170 1422 2809 2124 1557 111 118 1373 Cuts at: 12213 12339 13817 2529 Size: 126 1478 4400 Fragments arranged by size: 4400 2809 2124 1557 1478 1422 1373 170 126 118 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 13496 276 Size: 53 83 186 936 11962 2468 Fragments arranged by size: 11962 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BbeI BsiWI FseI FspAI KasI NarI NruI PacI PmlI PspXI RsrII SanDI SfiI SfoI SrfI SwaI