(Linear) (Six-base) MAPSORT of: KIBB9894.seq Check: 2858 from: 1 to: 10983 KIBB9894.orf With 182 enzymes: SgfI * December 22, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 8455 9033 9781 10983 Size: 8455 578 748 1202 Fragments arranged by size: 8455 1202 748 578 AccI GT'mk_AC Cuts at: 0 1746 1811 2346 4195 8551 9344 10150 10983 Size: 1746 65 535 1849 4356 793 806 833 Fragments arranged by size: 4356 1849 1746 833 806 793 535 65 Acc65I G'GTAC_C Cuts at: 0 1477 5587 9109 10983 Size: 1477 4110 3522 1874 Fragments arranged by size: 4110 3522 1874 1477 AclI AA'CG_TT Cuts at: 0 8238 10983 Size: 8238 2745 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1230 2737 3278 3362 3838 4116 4808 Size: 604 626 1507 541 84 476 278 692 Cuts at: 4808 4859 4973 5053 5198 5519 5603 6184 7586 Size: 51 114 80 145 321 84 581 1402 Cuts at: 7586 8485 8545 8704 9081 10983 Size: 899 60 159 377 1902 Fragments arranged by size: 1902 1507 1402 899 692 626 604 581 541 476 377 321 278 159 145 114 84 84 80 60 51 AflII C'TTAA_G Cuts at: 0 2152 8730 10983 Size: 2152 6578 2253 Fragments arranged by size: 6578 2253 2152 AflIII A'CryG_T Cuts at: 0 3827 5921 8633 10452 10983 Size: 3827 2094 2712 1819 531 Fragments arranged by size: 3827 2712 2094 1819 531 AleI CACnn'nnGTG Cuts at: 0 3386 10983 Size: 3386 7597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3591 6965 7946 10983 Size: 3591 3374 981 3037 Fragments arranged by size: 3591 3374 3037 981 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2641 2673 4226 4258 4910 4942 7034 7066 Size: 2641 32 1553 32 652 32 2092 32 Cuts at: 7066 9419 9451 10983 Size: 2353 32 1532 Fragments arranged by size: 2641 2353 2092 1553 1532 652 32 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1092 2676 2728 2958 5418 8463 9104 10042 Size: 1092 1584 52 230 2460 3045 641 938 Cuts at: 10042 10218 10741 10900 10983 Size: 176 523 159 83 Fragments arranged by size: 3045 2460 1584 1092 938 641 523 230 176 159 83 52 ApaI G_GGCC'C Cuts at: 0 4442 10983 Size: 4442 6541 ApaLI G'TGCA_C Cuts at: 0 1945 2419 10983 Size: 1945 474 8564 Fragments arranged by size: 8564 1945 474 ApoI r'AATT_y Cuts at: 0 183 520 715 1503 1822 2275 2510 2519 Size: 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270 52 BanII G_rGCy'C Cuts at: 0 15 2808 4442 4647 5894 6899 8012 8048 Size: 15 2793 1634 205 1247 1005 1113 36 Cuts at: 8048 10983 Size: 2935 Fragments arranged by size: 2935 2793 1634 1247 1113 1005 205 36 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 482 1103 2725 4814 5093 5768 6172 7999 Size: 482 621 1622 2089 279 675 404 1827 Cuts at: 7999 9230 10651 10983 Size: 1231 1421 332 Fragments arranged by size: 2089 1827 1622 1421 1231 675 621 482 404 332 279 BbvCI CC'TCA_GC Cuts at: 0 322 1115 1123 10983 Size: 322 793 8 9860 Fragments arranged by size: 9860 793 322 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 355 389 2983 3017 3583 3617 10983 Size: 355 34 2594 34 566 34 7366 Fragments arranged by size: 7366 2594 566 355 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 71 855 942 4751 5582 6976 7961 10983 Size: 71 784 87 3809 831 1394 985 3022 Fragments arranged by size: 3809 3022 1394 985 831 784 87 71 BclI T'GATC_A Cuts at: 0 2025 2871 10983 Size: 2025 846 8112 Fragments arranged by size: 8112 2025 846 BfrBI ATG'CAT Cuts at: 0 2989 3010 10983 Size: 2989 21 7973 Fragments arranged by size: 7973 2989 21 BglI GCCn_nnn'nGGC Cuts at: 0 3598 4873 10983 Size: 3598 1275 6110 Fragments arranged by size: 6110 3598 1275 BglII A'GATC_T Cuts at: 0 692 6955 9615 10983 Size: 692 6263 2660 1368 Fragments arranged by size: 6263 2660 1368 692 BlpI GC'TnA_GC Cuts at: 0 28 1657 4646 6796 10952 10983 Size: 28 1629 2989 2150 4156 31 Fragments arranged by size: 4156 2989 2150 1629 31 28 Bme1580I G_kGCm'C Cuts at: 0 1725 1851 1949 2423 4442 10983 Size: 1725 126 98 474 2019 6541 Fragments arranged by size: 6541 2019 1725 474 126 98 BmgBI CAC'GTC Cuts at: 0 594 4167 10983 Size: 594 3573 6816 Fragments arranged by size: 6816 3573 594 BmrI ACTGGGnnnn_n' Cuts at: 0 2806 7156 10802 10983 Size: 2806 4350 3646 181 Fragments arranged by size: 4350 3646 2806 181 BmtI G_CTAG'C Cuts at: 0 9751 10983 Size: 9751 1232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 10833 10983 Size: 10833 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1477 2989 3402 3803 5440 6490 7061 7178 Size: 1477 1512 413 401 1637 1050 571 117 Cuts at: 7178 7261 7558 7876 7933 8387 10450 10983 Size: 83 297 318 57 454 2063 533 Fragments arranged by size: 2063 1637 1512 1477 1050 571 533 454 413 401 318 297 117 83 57 Bpu10I CC'TnA_GC Cuts at: 0 322 1115 1123 2488 3444 7486 9706 10298 Size: 322 793 8 1365 956 4042 2220 592 Cuts at: 10298 10983 Size: 685 Fragments arranged by size: 4042 2220 1365 956 793 685 592 322 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2792 5531 8891 8993 9341 9766 10983 Size: 2792 2739 3360 102 348 425 1217 Fragments arranged by size: 3360 2792 2739 1217 425 348 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 26 479 7216 10775 10983 Size: 26 453 6737 3559 208 Fragments arranged by size: 6737 3559 453 208 26 BsaAI yAC'GTr Cuts at: 0 7791 10983 Size: 7791 3192 BsaBI GATnn'nnATC Cuts at: 0 6479 8983 9032 10874 10983 Size: 6479 2504 49 1842 109 Fragments arranged by size: 6479 2504 1842 109 49 BsaHI Gr'CG_yC Cuts at: 0 5124 10983 Size: 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3440 4647 5341 Size: 1658 291 45 429 385 632 1207 694 Cuts at: 5341 5426 5882 5894 6246 6899 7616 7875 7901 Size: 85 456 12 352 653 717 259 26 Cuts at: 7901 8012 8048 9336 10244 10983 Size: 111 36 1288 908 739 Fragments arranged by size: 1658 1288 1207 908 739 717 694 653 632 456 429 385 352 291 259 111 85 45 36 26 12 BsmI GAATG_Cn' Cuts at: 0 896 7708 7821 8887 9250 10677 10983 Size: 896 6812 113 1066 363 1427 306 Fragments arranged by size: 6812 1427 1066 896 363 306 113 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5928 10983 Size: 5928 5055 Bsp1286I G_dGCh'C Cuts at: 0 15 1658 1725 1851 1949 1994 2423 2808 Size: 15 1643 67 126 98 45 429 385 Cuts at: 2808 3440 4442 4647 5341 5426 5882 5894 6246 Size: 632 1002 205 694 85 456 12 352 Cuts at: 6246 6899 7616 7875 7901 8012 8048 9336 10244 Size: 653 717 259 26 111 36 1288 908 Cuts at: 10244 10983 Size: 739 Fragments arranged by size: 1643 1288 1002 908 739 717 694 653 632 456 429 385 352 259 205 126 111 98 85 67 45 36 26 15 12 BspEI T'CCGG_A Cuts at: 0 3837 10625 10983 Size: 3837 6788 358 Fragments arranged by size: 6788 3837 358 BspHI T'CATG_A Cuts at: 0 3101 6014 6105 8259 10983 Size: 3101 2913 91 2154 2724 Fragments arranged by size: 3101 2913 2724 2154 91 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1002 7182 8455 9033 9781 10983 Size: 1002 6180 1273 578 748 1202 Fragments arranged by size: 6180 1273 1202 1002 748 578 BsrBI CCG'CTC Cuts at: 0 8435 10983 Size: 8435 2548 BsrDI GCAATG_nn' Cuts at: 0 4505 5365 9350 10983 Size: 4505 860 3985 1633 Fragments arranged by size: 4505 3985 1633 860 BsrFI r'CCGG_y Cuts at: 0 5037 9038 10983 Size: 5037 4001 1945 Fragments arranged by size: 5037 4001 1945 BsrGI T'GTAC_A Cuts at: 0 585 1298 5228 9174 10983 Size: 585 713 3930 3946 1809 Fragments arranged by size: 3946 3930 1809 713 585 BssSI C'ACGA_G Cuts at: 0 2178 5386 5851 10983 Size: 2178 3208 465 5132 Fragments arranged by size: 5132 3208 2178 465 BstAPI GCAn_nnn'nTGC Cuts at: 0 4291 9796 10983 Size: 4291 5505 1187 Fragments arranged by size: 5505 4291 1187 BstBI TT'CG_AA Cuts at: 0 2841 7119 8575 10440 10983 Size: 2841 4278 1456 1865 543 Fragments arranged by size: 4278 2841 1865 1456 543 BstXI CCAn_nnnn'nTGG Cuts at: 0 1474 3222 10983 Size: 1474 1748 7761 Fragments arranged by size: 7761 1748 1474 BstYI r'GATC_y Cuts at: 0 277 692 1401 3840 4592 6955 8278 9615 Size: 277 415 709 2439 752 2363 1323 1337 Cuts at: 9615 10983 Size: 1368 Fragments arranged by size: 2439 2363 1368 1337 1323 752 709 415 277 BstZ17I GTA'TAC Cuts at: 0 4196 8552 10983 Size: 4196 4356 2431 Fragments arranged by size: 4356 4196 2431 Bsu36I CC'TnA_GG Cuts at: 0 1757 3539 10983 Size: 1757 1782 7444 Fragments arranged by size: 7444 1782 1757 BtgI C'CryG_G Cuts at: 0 64 2916 4174 5146 5452 5828 6289 7902 Size: 64 2852 1258 972 306 376 461 1613 Cuts at: 7902 7937 8510 9661 10983 Size: 35 573 1151 1322 Fragments arranged by size: 2852 1613 1322 1258 1151 972 573 461 376 306 64 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3972 10008 10983 Size: 3972 6036 975 Fragments arranged by size: 6036 3972 975 BtsI GCAGTG_nn' Cuts at: 0 1658 2746 3140 4350 4370 5250 5444 5636 Size: 1658 1088 394 1210 20 880 194 192 Cuts at: 5636 8108 8476 9500 10832 10983 Size: 2472 368 1024 1332 151 Fragments arranged by size: 2472 1658 1332 1210 1088 1024 880 394 368 194 192 151 20 ClaI AT'CG_AT Cuts at: 0 418 8780 10983 Size: 418 8362 2203 Fragments arranged by size: 8362 2203 418 DraI TTT'AAA Cuts at: 0 125 3911 4398 6213 10983 Size: 125 3786 487 1815 4770 Fragments arranged by size: 4770 3786 1815 487 125 DraIII CAC_nnn'GTG Cuts at: 0 5328 7150 8481 9317 10983 Size: 5328 1822 1331 836 1666 Fragments arranged by size: 5328 1822 1666 1331 836 EaeI y'GGCC_r Cuts at: 0 4865 5035 5360 7323 8868 9233 10638 10983 Size: 4865 170 325 1963 1545 365 1405 345 Fragments arranged by size: 4865 1963 1545 1405 365 345 325 170 EagI C'GGCC_G Cuts at: 0 5035 10983 Size: 5035 5948 EarI CTCTTCn'nnn_ Cuts at: 0 1082 2264 2367 3537 4135 4730 4985 5959 Size: 1082 1182 103 1170 598 595 255 974 Cuts at: 5959 5993 6329 6436 6833 6848 8000 8723 9047 Size: 34 336 107 397 15 1152 723 324 Cuts at: 9047 10834 10983 Size: 1787 149 Fragments arranged by size: 1787 1182 1170 1152 1082 974 723 598 595 397 336 324 255 149 107 103 34 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 6389 10983 Size: 6389 4594 EcoICRI GAG'CTC Cuts at: 0 2806 4645 5892 6897 8010 8046 10983 Size: 2806 1839 1247 1005 1113 36 2937 Fragments arranged by size: 2937 2806 1839 1247 1113 1005 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1230 1477 2737 2989 3278 3362 3402 Size: 604 626 247 1260 252 289 84 40 Cuts at: 3402 3803 3838 4116 4808 4859 4973 5053 5198 Size: 401 35 278 692 51 114 80 145 Cuts at: 5198 5440 5519 5603 6184 6490 7061 7178 7261 Size: 242 79 84 581 306 571 117 83 Cuts at: 7261 7558 7586 7876 7933 8387 8485 8545 8704 Size: 297 28 290 57 454 98 60 159 Cuts at: 8704 9081 10450 10983 Size: 377 1369 533 Fragments arranged by size: 1369 1260 692 626 604 581 571 533 454 401 377 306 297 290 289 278 252 247 242 159 145 117 114 98 84 84 83 80 79 60 57 51 40 35 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 2170 3190 10983 Size: 2170 1020 7793 Fragments arranged by size: 7793 2170 1020 EcoO109I rG'GnC_Cy Cuts at: 0 1119 1261 4438 10983 Size: 1119 142 3177 6545 Fragments arranged by size: 6545 3177 1119 142 EcoRI G'AATT_C Cuts at: 0 5939 8571 10983 Size: 5939 2632 2412 Fragments arranged by size: 5939 2632 2412 EcoRV GAT'ATC Cuts at: 0 5135 8499 10983 Size: 5135 3364 2484 Fragments arranged by size: 5135 3364 2484 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 819 2182 8272 9111 9537 9637 10983 Size: 819 1363 6090 839 426 100 1346 Fragments arranged by size: 6090 1363 1346 839 819 426 100 FspI TGC'GCA Cuts at: 0 5866 10983 Size: 5866 5117 HaeII r_GCGC'y Cuts at: 0 1900 7893 10983 Size: 1900 5993 3090 Fragments arranged by size: 5993 3090 1900 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 88 120 1917 1949 3083 3115 3752 3784 Size: 88 32 1797 32 1134 32 637 32 Cuts at: 3784 4744 4776 5794 5826 6338 6370 6886 6918 Size: 960 32 1018 32 512 32 516 32 Cuts at: 6918 10648 10680 10983 Size: 3730 32 303 Fragments arranged by size: 3730 1797 1134 1018 960 637 516 512 303 88 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 330 1358 2696 4490 4902 10136 10911 10983 Size: 330 1028 1338 1794 412 5234 775 72 Fragments arranged by size: 5234 1794 1338 1028 775 412 330 72 HindIII A'AGCT_T Cuts at: 0 4139 7488 7740 10983 Size: 4139 3349 252 3243 Fragments arranged by size: 4139 3349 3243 252 HpaI GTT'AAC Cuts at: 0 330 10983 Size: 330 10653 KpnI G_GTAC'C Cuts at: 0 1481 5591 9113 10983 Size: 1481 4110 3522 1870 Fragments arranged by size: 4110 3522 1870 1481 MfeI C'AATT_G Cuts at: 0 562 1859 2640 2825 3587 3874 10983 Size: 562 1297 781 185 762 287 7109 Fragments arranged by size: 7109 1297 781 762 562 287 185 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 280 948 1930 2627 3279 4199 4413 4761 Size: 280 668 982 697 652 920 214 348 Cuts at: 4761 5671 6960 7442 8847 10606 10948 10983 Size: 910 1289 482 1405 1759 342 35 Fragments arranged by size: 1759 1405 1289 982 920 910 697 668 652 482 348 342 280 214 35 MscI TGG'CCA Cuts at: 0 4867 5362 7325 8870 9235 10640 10983 Size: 4867 495 1963 1545 365 1405 343 Fragments arranged by size: 4867 1963 1545 1405 495 365 343 MslI CAynn'nnrTG Cuts at: 0 3220 3386 3573 5353 5399 5924 6485 6973 Size: 3220 166 187 1780 46 525 561 488 Cuts at: 6973 7765 7928 8119 8264 8610 10983 Size: 792 163 191 145 346 2373 Fragments arranged by size: 3220 2373 1780 792 561 525 488 346 191 187 166 163 145 46 MspA1I CmG'CkG Cuts at: 0 35 66 190 1363 2215 4059 4356 4626 Size: 35 31 124 1173 852 1844 297 270 Cuts at: 4626 4871 6085 6200 7854 8376 8866 9259 9459 Size: 245 1214 115 1654 522 490 393 200 Cuts at: 9459 9573 9582 10711 10956 10983 Size: 114 9 1129 245 27 Fragments arranged by size: 1844 1654 1214 1173 1129 852 522 490 393 297 270 245 245 200 124 115 114 35 31 27 9 NaeI GCC'GGC Cuts at: 0 5039 10983 Size: 5039 5944 NcoI C'CATG_G Cuts at: 0 2916 4174 5146 5452 5828 6289 8510 10983 Size: 2916 1258 972 306 376 461 2221 2473 Fragments arranged by size: 2916 2473 2221 1258 972 461 376 306 NdeI CA'TA_TG Cuts at: 0 1110 1281 1440 2749 10983 Size: 1110 171 159 1309 8234 Fragments arranged by size: 8234 1309 1110 171 159 NgoMIV G'CCGG_C Cuts at: 0 5037 10983 Size: 5037 5946 NheI G'CTAG_C Cuts at: 0 9747 10983 Size: 9747 1236 NsiI A_TGCA'T Cuts at: 0 2991 3012 10983 Size: 2991 21 7971 Fragments arranged by size: 7971 2991 21 NspI r_CATG'y Cuts at: 0 2339 2353 3572 3831 4498 5398 5925 6070 Size: 2339 14 1219 259 667 900 527 145 Cuts at: 6070 8770 8803 8901 10983 Size: 2700 33 98 2082 Fragments arranged by size: 2700 2339 2082 1219 900 667 527 259 145 98 33 14 PciI A'CATG_T Cuts at: 0 3827 5921 10983 Size: 3827 2094 5062 Fragments arranged by size: 5062 3827 2094 PflMI CCAn_nnn'nTGG Cuts at: 0 2676 4263 4853 6369 8969 10428 10741 10983 Size: 2676 1587 590 1516 2600 1459 313 242 Fragments arranged by size: 2676 2600 1587 1516 1459 590 313 242 PfoI T'CCnGG_A Cuts at: 0 6726 10466 10734 10983 Size: 6726 3740 268 249 Fragments arranged by size: 6726 3740 268 249 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4910 4942 7034 7066 8820 8852 9764 9796 Size: 4910 32 2092 32 1754 32 912 32 Cuts at: 9796 10983 Size: 1187 Fragments arranged by size: 4910 2092 1754 1187 912 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1119 1261 10983 Size: 1119 142 9722 Fragments arranged by size: 9722 1119 142 PshAI GACnn'nnGTC Cuts at: 0 7775 10079 10983 Size: 7775 2304 904 Fragments arranged by size: 7775 2304 904 PsiI TTA'TAA Cuts at: 0 3547 10983 Size: 3547 7436 PspOMI G'GGCC_C Cuts at: 0 4438 10983 Size: 4438 6545 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1277 1309 2034 2066 5825 5857 10983 Size: 1277 32 725 32 3759 32 5126 Fragments arranged by size: 5126 3759 1277 725 32 32 32 PstI C_TGCA'G Cuts at: 0 842 2333 2901 2954 3135 6096 6966 7526 Size: 842 1491 568 53 181 2961 870 560 Cuts at: 7526 7814 7883 7949 8132 8428 8702 9572 9910 Size: 288 69 66 183 296 274 870 338 Cuts at: 9910 9951 10620 10983 Size: 41 669 363 Fragments arranged by size: 2961 1491 870 870 842 669 568 560 363 338 296 288 274 183 181 69 66 53 41 PvuII CAG'CTG Cuts at: 0 190 1363 2215 4059 4356 4626 4871 6200 Size: 190 1173 852 1844 297 270 245 1329 Cuts at: 6200 7854 8376 8866 9259 9459 9582 10711 10956 Size: 1654 522 490 393 200 123 1129 245 Cuts at: 10956 10983 Size: 27 Fragments arranged by size: 1844 1654 1329 1173 1129 852 522 490 393 297 270 245 245 200 190 123 27 SacI G_AGCT'C Cuts at: 0 2808 4647 5894 6899 8012 8048 10983 Size: 2808 1839 1247 1005 1113 36 2935 Fragments arranged by size: 2935 2808 1839 1247 1113 1005 36 SacII CC_GC'GG Cuts at: 0 67 10983 Size: 67 10916 SapI GCTCTTCn'nnn_ Cuts at: 0 6329 6436 8000 10983 Size: 6329 107 1564 2983 Fragments arranged by size: 6329 2983 1564 107 ScaI AGT'ACT Cuts at: 0 1218 3967 9006 10983 Size: 1218 2749 5039 1977 Fragments arranged by size: 5039 2749 1977 1218 SexAI A'CCwGG_T Cuts at: 0 1331 8477 10983 Size: 1331 7146 2506 Fragments arranged by size: 7146 2506 1331 SfcI C'TryA_G Cuts at: 0 261 785 838 1628 2329 2661 2897 2950 Size: 261 524 53 790 701 332 236 53 Cuts at: 2950 3131 4763 6092 6962 7522 7624 7810 7879 Size: 181 1632 1329 870 560 102 186 69 Cuts at: 7879 7945 8049 8128 8424 8698 9076 9568 9906 Size: 66 104 79 296 274 378 492 338 Cuts at: 9906 9947 10616 10983 Size: 41 669 367 Fragments arranged by size: 1632 1329 870 790 701 669 560 524 492 378 367 338 332 296 274 261 236 186 181 104 102 79 69 66 53 53 41 SmaI CCC'GGG Cuts at: 0 17 472 10983 Size: 17 455 10511 Fragments arranged by size: 10511 455 17 SmlI C'TyrA_G Cuts at: 0 2152 2807 5546 8730 8906 9008 9356 9781 Size: 2152 655 2739 3184 176 102 348 425 Cuts at: 9781 10983 Size: 1202 Fragments arranged by size: 3184 2739 2152 1202 655 425 348 176 102 SpeI A'CTAG_T Cuts at: 0 552 2415 10983 Size: 552 1863 8568 Fragments arranged by size: 8568 1863 552 SphI G_CATG'C Cuts at: 0 2339 5398 10983 Size: 2339 3059 5585 Fragments arranged by size: 5585 3059 2339 SspI AAT'ATT Cuts at: 0 130 1671 1703 2147 5219 5664 10983 Size: 130 1541 32 444 3072 445 5319 Fragments arranged by size: 5319 3072 1541 445 444 130 32 StuI AGG'CCT Cuts at: 0 1762 2288 3361 7246 8725 10983 Size: 1762 526 1073 3885 1479 2258 Fragments arranged by size: 3885 2258 1762 1479 1073 526 StyI C'CwwG_G Cuts at: 0 2916 3026 4174 4413 5146 5305 5452 5828 Size: 2916 110 1148 239 733 159 147 376 Cuts at: 5828 6289 6996 7773 8510 10983 Size: 461 707 777 737 2473 Fragments arranged by size: 2916 2473 1148 777 737 733 707 461 376 239 159 147 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 503 1010 5457 7138 7633 8868 9328 10357 Size: 503 507 4447 1681 495 1235 460 1029 Cuts at: 10357 10968 10983 Size: 611 15 Fragments arranged by size: 4447 1681 1235 1029 611 507 503 495 460 15 TatI w'GTAC_w Cuts at: 0 585 1216 1298 3965 4827 5228 9004 9174 Size: 585 631 82 2667 862 401 3776 170 Cuts at: 9174 9819 10983 Size: 645 1164 Fragments arranged by size: 3776 2667 1164 862 645 631 585 401 170 82 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 45 77 2696 2728 3370 3402 4740 4772 Size: 45 32 2619 32 642 32 1338 32 Cuts at: 4772 5012 5044 5628 5660 6072 6104 6545 6577 Size: 240 32 584 32 412 32 441 32 Cuts at: 6577 10983 Size: 4406 Fragments arranged by size: 4406 2619 1338 642 584 441 412 240 45 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1429 6352 10915 10983 Size: 1429 4923 4563 68 Fragments arranged by size: 4923 4563 1429 68 XbaI T'CTAG_A Cuts at: 0 7393 7554 10938 10983 Size: 7393 161 3384 45 Fragments arranged by size: 7393 3384 161 45 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1135 3222 6622 8830 9807 10983 Size: 1135 2087 3400 2208 977 1176 Fragments arranged by size: 3400 2208 2087 1176 1135 977 XmaI C'CCGG_G Cuts at: 0 15 470 10983 Size: 15 455 10513 Fragments arranged by size: 10513 455 15 XmnI GAAnn'nnTTC Cuts at: 0 524 694 2116 4925 7049 8606 8717 8835 Size: 524 170 1422 2809 2124 1557 111 118 Cuts at: 8835 10208 10334 10983 Size: 1373 126 649 Fragments arranged by size: 2809 2124 1557 1422 1373 649 524 170 126 118 111 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AgeI AhdI AscI AsiSI BbeI BsiWI BssHII BstEII CspCI DrdI FseI FspAI KasI MluI NarI NotI NruI PacI PasI PmeI PmlI PspXI PvuI RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SrfI SwaI XhoI ZraI