Restriction map for
pFN21AE2305
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# NO CUT ENZYMES ( 43 enzymes) #
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AclI AcsI AflII ApoI AscI
AseI AsnI Asp700I BfrI BsaBI
Bsp1407I BspDI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV HindIII HpaI MamI MfeI
MunI NdeI NspV PacI PmeI
PshBI Psp1406I PvuI RsrII SbfI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AatII 3925 GACGTC GACGT^C
Acc65I 5360 GGTACC G^GTACC
AgeI 9038 ACCGGT A^CCGGT
Asp718I 5360 GGTACC G^GTACC
AvrII 4845 CCTAGG C^CTAGG
BclI 2517 TGATCA T^GATCA
BlnI 4845 CCTAGG C^CTAGG
BsiWI 1156 CGTACG C^GTACG
BspLU11I 3842 ACATGT A^CATGT
BstEII 2772 GGTGACC G^GT[ACGT]ACC
BstPI 2772 GGTGACC G^GT[ACGT]ACC
DrdI 3595 GACCCCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2772 GGTGACC G^GT[ACGT]ACC
EcoT22I 9309 ATGCAT ATGCA^T
FbaI 2517 TGATCA T^GATCA
KpnI 5360 GGTACC GGTAC^C
MluI 1035 ACGCGT A^CGCGT
MseI 2246 TTAA T^TAA
NheI 3996 GCTAGC G^CTAGC
NotI 12 GCGGCCGC GC^GGCCGC
NsiI 9309 ATGCAT ATGCA^T
PinAI 9038 ACCGGT A^CCGGT
SalI 42 GTCGAC G^TCGAC
ScaI 2060 AGTACT AGT^ACT
SgrAI 1059 CGCCGGCG C[GA]^CCGG[CT]G
StuI 5694 AGGCCT AGG^CCT
Tru9I 2246 TTAA T^TAA
XbaI 9202 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AspI 8557 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
9685 GACTCGGTC
AviII 1853 TGCGCA TGC^GCA
7691 TGCGCA
BamHI 7071 GGATCC G^GATCC
7718 GGATCC
BglII 2945 AGATCT A^GATCT
6400 AGATCT
BsmBI 8481 CGTCTC CGTCTC(1/5)
9875 CGTCTC
DraIII 3757 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
5806 CACCGAGTG
FseI 1876 GGCCGGCC GGCCGG^CC
4425 GGCCGGCC
FspI 1853 TGCGCA TGC^GCA
7691 TGCGCA
NruI 1326 TCGCGA TCG^CGA
4628 TCGCGA
PflFI 8557 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
9685 GACTCGGTC
PshAI 383 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3144 GACTCCAGTC
SfiI 5581 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
8281 GGCCTCCAGGGCC
SphI 824 GCATGC GCATG^C
7886 GCATGC
Tth111I 8557 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
9685 GACTCGGTC
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# 3 OR MORE CUT ENZYMES ( 173 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AspEI AspHI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI