Restriction map for
pFN21AE2305
(Linear) (Six-base) MAPSORT of: KIEE2305.seq Check: 7338 from: 1 to: 9936
KIEE2305.orf
With 182 enzymes: SgfI *
December 13, 2010 18:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1647 4474 5920 6002 6716 9936
Size: 1647 2827 1446 82 714 3220
Fragments arranged by size:
3220 2827 1647 1446 714 82
AatII G_ACGT'C
Cuts at: 0 3929 9936
Size: 3929 6007
AccI GT'mk_AC
Cuts at: 0 43 1082 2342 9936
Size: 43 1039 1260 7594
Fragments arranged by size:
7594 1260 1039 43
Acc65I G'GTAC_C
Cuts at: 0 5360 9936
Size: 5360 4576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 926 5214 5689 7145 8714 9052 9936
Size: 500 426 4288 475 1456 1569 338 884
Fragments arranged by size:
4288 1569 1456 884 500 475 426 338
AfeI AGC'GCT
Cuts at: 0 1645 3856 5218 9936
Size: 1645 2211 1362 4718
Fragments arranged by size:
4718 2211 1645 1362
AflIII A'CryG_T
Cuts at: 0 1035 3842 4130 4370 9936
Size: 1035 2807 288 240 5566
Fragments arranged by size:
5566 2807 1035 288 240
AgeI A'CCGG_T
Cuts at: 0 9038 9936
Size: 9038 898
AhdI GACnn_n'nnGTC
Cuts at: 0 1224 2148 8844 9579 9936
Size: 1224 924 6696 735 357
Fragments arranged by size:
6696 1224 924 735 357
AleI CACnn'nnGTG
Cuts at: 0 1027 1252 1687 2320 2629 3271 5174 6053
Size: 1027 225 435 633 309 642 1903 879
Cuts at: 6053 7084 7630 9936
Size: 1031 546 2306
Fragments arranged by size:
2306 1903 1031 1027 879 642 633 546
435 309 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1045 1382 3661 4946 7512 9376 9936
Size: 1045 337 2279 1285 2566 1864 560
Fragments arranged by size:
2566 2279 1864 1285 1045 560 337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 326 358 9936
Size: 326 32 9578
Fragments arranged by size:
9578 326 32
AlwNI CAG_nnn'CTG
Cuts at: 0 391 766 996 2956 3654 5667 5733 6362
Size: 391 375 230 1960 698 2013 66 629
Cuts at: 6362 7207 7872 8292 8377 8398 8587 9841 9936
Size: 845 665 420 85 21 189 1254 95
Fragments arranged by size:
2013 1960 1254 845 698 665 629 420
391 375 230 189 95 85 66 21
ApaI G_GGCC'C
Cuts at: 0 302 4034 4531 4864 5008 5861 6468 6852
Size: 302 3732 497 333 144 853 607 384
Cuts at: 6852 7020 9009 9808 9854 9936
Size: 168 1989 799 46 82
Fragments arranged by size:
3732 1989 853 799 607 497 384 333
302 168 144 82 46
ApaLI G'TGCA_C
Cuts at: 0 1738 2530 3463 4853 6266 7507 7803 9936
Size: 1738 792 933 1390 1413 1241 296 2133
Fragments arranged by size:
2133 1738 1413 1390 1241 933 792 296
AvaI C'yCGr_G
Cuts at: 0 31 486 492 714 727 805 833 871
Size: 31 455 6 222 13 78 28 38
Cuts at: 871 885 1564 2183 2798 3886 5038 5487 6458
Size: 14 679 619 615 1088 1152 449 971
Cuts at: 6458 7010 8333 9068 9380 9446 9485 9599 9936
Size: 552 1323 735 312 66 39 114 337
Fragments arranged by size:
1323 1152 1088 971 735 679 619 615
552 455 449 337 312 222 114 78
66 39 38 31 28 14 13 6
AvrII C'CTAG_G
Cuts at: 0 4845 9936
Size: 4845 5091
BamHI G'GATC_C
Cuts at: 0 7071 7718 9936
Size: 7071 647 2218
Fragments arranged by size:
7071 2218 647
BanI G'GyrC_C
Cuts at: 0 113 404 533 598 813 999 1021 1073
Size: 113 291 129 65 215 186 22 52
Cuts at: 1073 1815 2079 2314 3967 4462 4516 4667 5140
Size: 742 264 235 1653 495 54 151 473
Cuts at: 5140 5360 5990 6047 6887 7288 7918 8470 8915
Size: 220 630 57 840 401 630 552 445
Cuts at: 8915 9057 9073 9092 9394 9441 9618 9629 9936
Size: 142 16 19 302 47 177 11 307
Fragments arranged by size:
1653 840 742 630 630 552 495 473
445 401 307 302 291 264 235 220
215 186 177 151 142 129 113 65
57 54 52 47 22 19 16 11
BanII G_rGCy'C
Cuts at: 0 263 302 471 493 3041 3250 4034 4148
Size: 263 39 169 22 2548 209 784 114
Cuts at: 4148 4307 4531 4864 5008 5488 5861 6006 6103
Size: 159 224 333 144 480 373 145 97
Cuts at: 6103 6250 6306 6468 6518 6557 6852 6862 7020
Size: 147 56 162 50 39 295 10 158
Cuts at: 7020 7760 8042 8225 8908 9009 9341 9592 9808
Size: 740 282 183 683 101 332 251 216
Cuts at: 9808 9854 9936
Size: 46 82
Fragments arranged by size:
2548 784 740 683 480 373 333 332
295 282 263 251 224 216 209 183
169 162 159 158 147 145 144 114
101 97 82 56 50 46 39 39
22 10
BbeI G_GCGC'C
Cuts at: 0 408 817 1077 4520 4671 7922 8474 8919
Size: 408 409 260 3443 151 3251 552 445
Cuts at: 8919 9398 9936
Size: 479 538
Fragments arranged by size:
3443 3251 552 538 479 445 409 408
260 151
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 694 3189 3921 8158 9936
Size: 191 503 2495 732 4237 1778
Fragments arranged by size:
4237 2495 1778 732 503 191
BbvCI CC'TCA_GC
Cuts at: 0 85 2214 3206 3920 7840 9936
Size: 85 2129 992 714 3920 2096
Fragments arranged by size:
3920 2129 2096 992 714 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4528 4562 7422 7456 7794 7828 9361 9395
Size: 4528 34 2860 34 338 34 1533 34
Cuts at: 9395 9936
Size: 541
Fragments arranged by size:
4528 2860 1533 541 338 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3166 3549 5064 6594 9936
Size: 3166 383 1515 1530 3342
Fragments arranged by size:
3342 3166 1530 1515 383
BclI T'GATC_A
Cuts at: 0 2517 9936
Size: 2517 7419
BfrBI ATG'CAT
Cuts at: 0 9311 9936
Size: 9311 625
BglI GCCn_nnn'nGGC
Cuts at: 0 21 1066 1796 4511 5249 5588 6086 7732
Size: 21 1045 730 2715 738 339 498 1646
Cuts at: 7732 8288 8537 9369 9564 9627 9936
Size: 556 249 832 195 63 309
Fragments arranged by size:
2715 1646 1045 832 738 730 556 498
339 309 249 195 63 21
BglII A'GATC_T
Cuts at: 0 2945 6400 9936
Size: 2945 3455 3536
Fragments arranged by size:
3536 3455 2945
BlpI GC'TnA_GC
Cuts at: 0 135 4888 6626 6976 7684 8580 9936
Size: 135 4753 1738 350 708 896 1356
Fragments arranged by size:
4753 1738 1356 896 708 350 135
Bme1580I G_kGCm'C
Cuts at: 0 116 302 601 939 1742 2317 2511 2534
Size: 116 186 299 338 803 575 194 23
Cuts at: 2534 2583 3134 3467 3970 4034 4465 4507 4531
Size: 49 551 333 503 64 431 42 24
Cuts at: 4531 4857 4864 5008 5861 6050 6082 6270 6289
Size: 326 7 144 853 189 32 188 19
Cuts at: 6289 6452 6468 6852 7020 7511 7807 8502 9009
Size: 163 16 384 168 491 296 695 507
Cuts at: 9009 9078 9166 9444 9808 9854 9936
Size: 69 88 278 364 46 82
Fragments arranged by size:
853 803 695 575 551 507 503 491
431 384 364 338 333 326 299 296
278 194 189 188 186 168 163 144
116 88 82 69 64 49 46 42
32 24 23 19 16 7
BmgBI CAC'GTC
Cuts at: 0 2658 2859 3468 8640 9875 9936
Size: 2658 201 609 5172 1235 61
Fragments arranged by size:
5172 2658 1235 609 201 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 2700 4559 6995 7095 7260 9936
Size: 2700 1859 2436 100 165 2676
Fragments arranged by size:
2700 2676 2436 1859 165 100
BmtI G_CTAG'C
Cuts at: 0 4000 9936
Size: 4000 5936
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 435 779 3060 9936
Size: 435 344 2281 6876
Fragments arranged by size:
6876 2281 435 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 2710 3131 3383 5578 6532 6913 7396
Size: 1138 1572 421 252 2195 954 381 483
Cuts at: 7396 7615 7627 8070 8269 8593 9355 9936
Size: 219 12 443 199 324 762 581
Fragments arranged by size:
2195 1572 1138 954 762 581 483 443
421 381 324 252 219 199 12
Bpu10I CC'TnA_GC
Cuts at: 0 85 180 305 1046 2214 3206 3335 3920
Size: 85 95 125 741 1168 992 129 585
Cuts at: 3920 7840 9080 9286 9936
Size: 3920 1240 206 650
Fragments arranged by size:
3920 1240 1168 992 741 650 585 206
129 125 95 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3856 4803 7152 7684 9525 9936
Size: 3856 947 2349 532 1841 411
Fragments arranged by size:
3856 2349 1841 947 532 411
BsaI GGTCTCn'nnnn_
Cuts at: 0 420 844 1312 2127 2180 2913 4291 5613
Size: 420 424 468 815 53 733 1378 1322
Cuts at: 5613 9936
Size: 4323
Fragments arranged by size:
4323 1378 1322 815 733 468 424 420
53
BsaAI yAC'GTr
Cuts at: 0 1650 4006 4071 4373 5688 7632 9936
Size: 1650 2356 65 302 1315 1944 2304
Fragments arranged by size:
2356 2304 1944 1650 1315 302 65
BsaHI Gr'CG_yC
Cuts at: 0 341 405 814 1058 1074 1724 3926 4445
Size: 341 64 409 244 16 650 2202 519
Cuts at: 4445 4517 4668 6434 6961 7919 7952 8471 8916
Size: 72 151 1766 527 958 33 519 445
Cuts at: 8916 9395 9936
Size: 479 541
Fragments arranged by size:
2202 1766 958 650 541 527 519 519
479 445 409 341 244 151 72 64
33 16
BsaWI w'CCGG_w
Cuts at: 0 192 667 3140 4396 9038 9936
Size: 192 475 2473 1256 4642 898
Fragments arranged by size:
4642 2473 1256 898 475 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 44 74 627 657 3423 3453 4051 4081
Size: 44 30 553 30 2766 30 598 30
Cuts at: 4081 6520 6550 9453 9483 9936
Size: 2439 30 2903 30 453
Fragments arranged by size:
2903 2766 2439 598 553 453 44 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 109 334 408 1354 1616 3223 3817
Size: 58 51 225 74 946 262 1607 594
Cuts at: 3817 4063 5714 5731 6910 7219 7750 8095 8395
Size: 246 1651 17 1179 309 531 345 300
Cuts at: 8395 8455 8475 8716 8719 8724 8797 9214 9753
Size: 60 20 241 3 5 73 417 539
Cuts at: 9753 9765 9936
Size: 12 171
Fragments arranged by size:
1651 1607 1179 946 594 539 531 417
345 309 300 262 246 241 225 171
74 73 60 58 51 20 17 12
5 3
BseYI C'CCAG_C
Cuts at: 0 99 122 324 560 571 2117 3201 3585
Size: 99 23 202 236 11 1546 1084 384
Cuts at: 3585 3873 5219 5419 5800 6837 7770 7946 7968
Size: 288 1346 200 381 1037 933 176 22
Cuts at: 7968 8403 9008 9527 9700 9936
Size: 435 605 519 173 236
Fragments arranged by size:
1546 1346 1084 1037 933 605 519 435
384 381 288 236 236 202 200 176
173 99 23 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3190 3445 3739 4452 4657 5074 5434 5732
Size: 3190 255 294 713 205 417 360 298
Cuts at: 5732 6782 7462 7810 7858 7885 8144 9119 9305
Size: 1050 680 348 48 27 259 975 186
Cuts at: 9305 9936
Size: 631
Fragments arranged by size:
3190 1050 975 713 680 631 417 360
348 298 294 259 255 205 186 48
27
BsiEI CG_ry'CG
Cuts at: 0 16 43 1275 1497 2566 4431 6202 8511
Size: 16 27 1232 222 1069 1865 1771 2309
Cuts at: 8511 9144 9568 9936
Size: 633 424 368
Fragments arranged by size:
2309 1865 1771 1232 1069 633 424 368
222 27 16
BsiHKAI G_wGCw'C
Cuts at: 0 1514 1742 2534 3467 3506 3908 3977 4181
Size: 1514 228 792 933 39 402 69 204
Cuts at: 4181 4307 4857 6062 6270 6374 6557 6998 7511
Size: 126 550 1205 208 104 183 441 513
Cuts at: 7511 7760 7807 8892 9463 9592 9936
Size: 249 47 1085 571 129 344
Fragments arranged by size:
1514 1205 1085 933 792 571 550 513
441 402 344 249 228 208 204 183
129 126 104 69 47 39
BsiWI C'GTAC_G
Cuts at: 0 1156 9936
Size: 1156 8780
BsmI GAATG_Cn'
Cuts at: 0 2052 2673 5820 9313 9936
Size: 2052 621 3147 3493 623
Fragments arranged by size:
3493 3147 2052 623 621
BsmBI CGTCTCn'nnnn_
Cuts at: 0 8487 9881 9936
Size: 8487 1394 55
Fragments arranged by size:
8487 1394 55
Bsp1286I G_dGCh'C
Cuts at: 0 116 263 302 471 493 601 939 1514
Size: 116 147 39 169 22 108 338 575
Cuts at: 1514 1742 2317 2511 2534 2583 3041 3134 3250
Size: 228 575 194 23 49 458 93 116
Cuts at: 3250 3467 3506 3908 3970 3977 4034 4148 4181
Size: 217 39 402 62 7 57 114 33
Cuts at: 4181 4307 4465 4507 4531 4857 4864 5008 5488
Size: 126 158 42 24 326 7 144 480
Cuts at: 5488 5861 6006 6050 6062 6082 6103 6250 6270
Size: 373 145 44 12 20 21 147 20
Cuts at: 6270 6289 6306 6374 6452 6468 6518 6557 6852
Size: 19 17 68 78 16 50 39 295
Cuts at: 6852 6862 6998 7020 7511 7760 7807 8042 8225
Size: 10 136 22 491 249 47 235 183
Cuts at: 8225 8502 8892 8908 9009 9078 9166 9341 9444
Size: 277 390 16 101 69 88 175 103
Cuts at: 9444 9463 9592 9808 9854 9936
Size: 19 129 216 46 82
Fragments arranged by size:
575 575 491 480 458 402 390 373
338 326 295 277 249 235 228 217
216 194 183 175 169 158 147 147
145 144 136 129 126 116 116 114
108 103 101 93 88 82 78 69
68 62 57 50 49 47 46 44
42 39 39 39 33 24 23 22
22 21 20 20 19 19 17 16
16 12 10 7 7
BspEI T'CCGG_A
Cuts at: 0 192 667 3140 9936
Size: 192 475 2473 6796
Fragments arranged by size:
6796 2473 475 192
BspHI T'CATG_A
Cuts at: 0 1100 2514 7313 9936
Size: 1100 1414 4799 2623
Fragments arranged by size:
4799 2623 1414 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1208 1409 1647 2708 4474 5096 5920 6002
Size: 1208 201 238 1061 1766 622 824 82
Cuts at: 6002 6716 7881 9227 9936
Size: 714 1165 1346 709
Fragments arranged by size:
1766 1346 1208 1165 1061 824 714 709
622 238 201 82
BsrBI CCG'CTC
Cuts at: 0 1112 1145 4646 8504 9936
Size: 1112 33 3501 3858 1432
Fragments arranged by size:
3858 3501 1432 1112 33
BsrDI GCAATG_nn'
Cuts at: 0 3645 4801 8841 9936
Size: 3645 1156 4040 1095
Fragments arranged by size:
4040 3645 1156 1095
BsrFI r'CCGG_y
Cuts at: 0 549 810 1060 1290 1877 2565 4426 5537
Size: 549 261 250 230 587 688 1861 1111
Cuts at: 5537 5582 5987 6790 9038 9290 9626 9936
Size: 45 405 803 2248 252 336 310
Fragments arranged by size:
2248 1861 1111 803 688 587 549 405
336 310 261 252 250 230 45
BssHII G'CGCG_C
Cuts at: 0 604 1432 1434 4349 4549 9936
Size: 604 828 2 2915 200 5387
Fragments arranged by size:
5387 2915 828 604 200 2
BssSI C'ACGA_G
Cuts at: 0 4705 8035 9638 9936
Size: 4705 3330 1603 298
Fragments arranged by size:
4705 3330 1603 298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2029 3777 4806 5550 6805 7872 8123 9936
Size: 2029 1748 1029 744 1255 1067 251 1813
Fragments arranged by size:
2029 1813 1748 1255 1067 1029 744 251
BstEII G'GTnAC_C
Cuts at: 0 2772 9936
Size: 2772 7164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1251 1254 1734 3673 4755 5302 5646 6089
Size: 1251 3 480 1939 1082 547 344 443
Cuts at: 6089 6621 6954 8634 8826 9936
Size: 532 333 1680 192 1110
Fragments arranged by size:
1939 1680 1251 1110 1082 547 532 480
443 344 333 192 3
BstYI r'GATC_y
Cuts at: 0 718 1802 2945 3518 6400 7071 7211 7718
Size: 718 1084 1143 573 2882 671 140 507
Cuts at: 7718 8024 9936
Size: 306 1912
Fragments arranged by size:
2882 1912 1143 1084 718 671 573 507
306 140
Bsu36I CC'TnA_GG
Cuts at: 0 477 5726 7245 7363 7994 8378 8551 9936
Size: 477 5249 1519 118 631 384 173 1385
Fragments arranged by size:
5249 1519 1385 631 477 384 173 118
BtgI C'CryG_G
Cuts at: 0 19 537 1025 1221 1767 2037 2190 4736
Size: 19 518 488 196 546 270 153 2546
Cuts at: 4736 4754 4913 5144 5301 5356 5639 6009 6082
Size: 18 159 231 157 55 283 370 73
Cuts at: 6082 6202 6605 6947 7108 7347 7914 9364 9398
Size: 120 403 342 161 239 567 1450 34
Cuts at: 9398 9705 9936
Size: 307 231
Fragments arranged by size:
2546 1450 567 546 518 488 403 370
342 307 283 270 239 231 231 196
161 159 157 153 120 73 55 34
19 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4230 9936
Size: 4230 5706
BtsI GCAGTG_nn'
Cuts at: 0 2520 3122 3266 3278 3778 5715 6082 6177
Size: 2520 602 144 12 500 1937 367 95
Cuts at: 6177 6178 6216 6289 6686 6799 7450 7805 8502
Size: 1 38 73 397 113 651 355 697
Cuts at: 8502 9815 9936
Size: 1313 121
Fragments arranged by size:
2520 1937 1313 697 651 602 500 397
367 355 144 121 113 95 73 38
12 1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1749 1784 5181 5216 7695 7730 9936
Size: 1749 35 3397 35 2479 35 2206
Fragments arranged by size:
3397 2479 2206 1749 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3762 5811 9936
Size: 3762 2049 4125
Fragments arranged by size:
4125 3762 2049
DrdI GACnn_nn'nnGTC
Cuts at: 0 3601 9936
Size: 3601 6335
EaeI y'GGCC_r
Cuts at: 0 13 1241 1256 1494 1577 1703 1875 1879
Size: 13 1228 15 238 83 126 172 4
Cuts at: 1879 1907 2563 2611 2979 3812 4428 4713 4910
Size: 28 656 48 368 833 616 285 197
Cuts at: 4910 5100 5535 5580 6309 6440 6648 7111 8508
Size: 190 435 45 729 131 208 463 1397
Cuts at: 8508 8642 8748 8773 8854 9292 9405 9565 9936
Size: 134 106 25 81 438 113 160 371
Fragments arranged by size:
1397 1228 833 729 656 616 463 438
435 371 368 285 238 208 197 190
172 160 134 131 126 113 106 83
81 48 45 28 25 15 13 4
EagI C'GGCC_G
Cuts at: 0 13 1494 2563 4428 8508 9565 9936
Size: 13 1481 1069 1865 4080 1057 371
Fragments arranged by size:
4080 1865 1481 1069 1057 371 13
EarI CTCTTCn'nnn_
Cuts at: 0 847 3529 3622 4660 5015 5632 6529 7768
Size: 847 2682 93 1038 355 617 897 1239
Cuts at: 7768 7864 8314 9204 9936
Size: 96 450 890 732
Fragments arranged by size:
2682 1239 1038 897 890 847 732 617
450 355 96 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 188 444 708 3460 4355 7480 9461 9936
Size: 188 256 264 2752 895 3125 1981 475
Fragments arranged by size:
3125 2752 1981 895 475 264 256 188
EcoICRI GAG'CTC
Cuts at: 0 4305 6555 7758 9590 9936
Size: 4305 2250 1203 1832 346
Fragments arranged by size:
4305 2250 1832 1203 346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 926 1138 2710 3131 3383 5214 5578
Size: 500 426 212 1572 421 252 1831 364
Cuts at: 5578 5689 6532 6913 7145 7396 7615 7627 8070
Size: 111 843 381 232 251 219 12 443
Cuts at: 8070 8269 8593 8714 9052 9355 9936
Size: 199 324 121 338 303 581
Fragments arranged by size:
1831 1572 843 581 500 443 426 421
381 364 338 324 303 252 251 232
219 212 199 121 111 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 770 3284 5629 9936
Size: 88 682 2514 2345 4307
Fragments arranged by size:
4307 2514 2345 682 88
EcoO109I rG'GnC_Cy
Cuts at: 0 27 126 223 298 299 436 483 710
Size: 27 99 97 75 1 137 47 227
Cuts at: 710 1050 2748 3289 4030 4031 4527 4860 4861
Size: 340 1698 541 741 1 496 333 1
Cuts at: 4861 5005 5085 5185 5400 5664 5858 5886 6464
Size: 144 80 100 215 264 194 28 578
Cuts at: 6464 6465 6849 7016 7449 7520 7553 7563 7784
Size: 1 384 167 433 71 33 10 221
Cuts at: 7784 8289 8407 8414 9299 9489 9546 9805 9851
Size: 505 118 7 885 190 57 259 46
Cuts at: 9851 9936
Size: 85
Fragments arranged by size:
1698 885 741 578 541 505 496 433
384 340 333 264 259 227 221 215
194 190 167 144 137 118 100 99
97 85 80 75 71 57 47 46
33 28 27 10 7 1 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4704 5187 9936
Size: 4704 483 4749
Fragments arranged by size:
4749 4704 483
FseI GG_CCGG'CC
Cuts at: 0 1881 4430 9936
Size: 1881 2549 5506
Fragments arranged by size:
5506 2549 1881
FspI TGC'GCA
Cuts at: 0 1855 7693 9936
Size: 1855 5838 2243
Fragments arranged by size:
5838 2243 1855
HaeII r_GCGC'y
Cuts at: 0 284 408 817 870 1077 1647 2111 3858
Size: 284 124 409 53 207 570 464 1747
Cuts at: 3858 3997 4025 4087 4520 4671 5220 6838 7922
Size: 139 28 62 433 151 549 1618 1084
Cuts at: 7922 8377 8474 8766 8919 9015 9398 9936
Size: 455 97 292 153 96 383 538
Fragments arranged by size:
1747 1618 1084 570 549 538 464 455
433 409 383 292 284 207 153 151
139 124 97 96 62 53 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 1210 1242 1456 1488 2134 2166
Size: 627 32 551 32 214 32 646 32
Cuts at: 2166 2179 2211 2430 2462 2710 2742 3073 3105
Size: 13 32 219 32 248 32 331 32
Cuts at: 3105 3343 3375 4992 5024 7404 7436 7776 7808
Size: 238 32 1617 32 2380 32 340 32
Cuts at: 7808 8662 8694 8830 8862 9565 9597 9936
Size: 854 32 136 32 703 32 339
Fragments arranged by size:
2380 1617 854 703 646 627 551 340
339 331 248 238 219 214 136 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 44 3104 3480 3663 8235 9936
Size: 44 3060 376 183 4572 1701
Fragments arranged by size:
4572 3060 1701 376 183 44
KasI G'GCGC_C
Cuts at: 0 404 813 1073 4516 4667 7918 8470 8915
Size: 404 409 260 3443 151 3251 552 445
Cuts at: 8915 9394 9936
Size: 479 542
Fragments arranged by size:
3443 3251 552 542 479 445 409 404
260 151
KpnI G_GTAC'C
Cuts at: 0 5364 9936
Size: 5364 4572
MluI A'CGCG_T
Cuts at: 0 1035 9936
Size: 1035 8901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1916 3566 3632 4254 4346 4772 8699 8793
Size: 1916 1650 66 622 92 426 3927 94
Cuts at: 8793 9707 9936
Size: 914 229
Fragments arranged by size:
3927 1916 1650 914 622 426 229 94
92 66
MscI TGG'CCA
Cuts at: 0 1909 3814 4715 6442 6650 8644 8856 9936
Size: 1909 1905 901 1727 208 1994 212 1080
Fragments arranged by size:
1994 1909 1905 1727 1080 901 212 208
MslI CAynn'nnrTG
Cuts at: 0 167 1027 1249 1252 1600 1687 1918 2320
Size: 167 860 222 3 348 87 231 402
Cuts at: 2320 2525 2629 2975 3271 3733 5096 5174 5300
Size: 205 104 346 296 462 1363 78 126
Cuts at: 5300 5344 5644 6053 6770 7082 7084 7514 7630
Size: 44 300 409 717 312 2 430 116
Cuts at: 7630 7727 8083 8657 9936
Size: 97 356 574 1279
Fragments arranged by size:
1363 1279 860 717 574 462 430 409
402 356 348 346 312 300 296 231
222 205 167 126 116 104 97 87
78 44 3 2
MspA1I CmG'CkG
Cuts at: 0 564 848 1027 1362 1429 1444 1493 1887
Size: 564 284 179 335 67 15 49 394
Cuts at: 1887 2308 3205 3651 3870 3919 4265 4433 5234
Size: 421 897 446 219 49 346 168 801
Cuts at: 5234 6204 6278 6326 6359 6630 6846 7797 7916
Size: 970 74 48 33 271 216 951 119
Cuts at: 7916 8535 8587 9130 9213 9531 9570 9609 9666
Size: 619 52 543 83 318 39 39 57
Cuts at: 9666 9936
Size: 270
Fragments arranged by size:
970 951 897 801 619 564 543 446
421 394 346 335 318 284 271 270
219 216 179 168 119 83 74 67
57 52 49 49 48 39 39 33
15
NaeI GCC'GGC
Cuts at: 0 812 1062 1879 4428 5584 5989 6792 9292
Size: 812 250 817 2549 1156 405 803 2500
Cuts at: 9292 9628 9936
Size: 336 308
Fragments arranged by size:
2549 2500 1156 817 812 803 405 336
308 250
NarI GG'CG_CC
Cuts at: 0 405 814 1074 4517 4668 7919 8471 8916
Size: 405 409 260 3443 151 3251 552 445
Cuts at: 8916 9395 9936
Size: 479 541
Fragments arranged by size:
3443 3251 552 541 479 445 409 405
260 151
NcoI C'CATG_G
Cuts at: 0 2037 2190 5144 5356 5639 6009 6605 6947
Size: 2037 153 2954 212 283 370 596 342
Cuts at: 6947 9398 9705 9936
Size: 2451 307 231
Fragments arranged by size:
2954 2451 2037 596 370 342 307 283
231 212 153
NgoMIV G'CCGG_C
Cuts at: 0 810 1060 1877 4426 5582 5987 6790 9290
Size: 810 250 817 2549 1156 405 803 2500
Cuts at: 9290 9626 9936
Size: 336 310
Fragments arranged by size:
2549 2500 1156 817 810 803 405 336
310 250
NheI G'CTAG_C
Cuts at: 0 3996 9936
Size: 3996 5940
NotI GC'GGCC_GC
Cuts at: 0 13 9936
Size: 13 9923
NruI TCG'CGA
Cuts at: 0 1328 4630 9936
Size: 1328 3302 5306
Fragments arranged by size:
5306 3302 1328
NsiI A_TGCA'T
Cuts at: 0 9313 9936
Size: 9313 623
NspI r_CATG'y
Cuts at: 0 828 3846 7890 9936
Size: 828 3018 4044 2046
Fragments arranged by size:
4044 3018 2046 828
PasI CC'CwG_GG
Cuts at: 0 2418 6468 6501 6853 7743 7941 8862 9936
Size: 2418 4050 33 352 890 198 921 1074
Fragments arranged by size:
4050 2418 1074 921 890 352 198 33
PciI A'CATG_T
Cuts at: 0 3842 9936
Size: 3842 6094
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 996 1812 1923 2117 3165 3318 6109
Size: 351 645 816 111 194 1048 153 2791
Cuts at: 6109 6711 7207 7941 8292 8398 9936
Size: 602 496 734 351 106 1538
Fragments arranged by size:
2791 1538 1048 816 734 645 602 496
351 351 194 153 111 106
PfoI T'CCnGG_A
Cuts at: 0 209 2687 5630 7373 9936
Size: 209 2478 2943 1743 2563
Fragments arranged by size:
2943 2563 2478 1743 209
PmlI CAC'GTG
Cuts at: 0 4006 4373 5688 7632 9936
Size: 4006 367 1315 1944 2304
Fragments arranged by size:
4006 2304 1944 1315 367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 9936
Size: 1096 32 8808
Fragments arranged by size:
8808 1096 32
PpuMI rG'GwC_Cy
Cuts at: 0 126 436 483 1050 5085 5400 5886 7449
Size: 126 310 47 567 4035 315 486 1563
Cuts at: 7449 7520 7553 7563 7784 8407 9299 9489 9546
Size: 71 33 10 221 623 892 190 57
Cuts at: 9546 9936
Size: 390
Fragments arranged by size:
4035 1563 892 623 567 486 390 315
310 221 190 126 71 57 47 33
10
PshAI GACnn'nnGTC
Cuts at: 0 387 3148 9936
Size: 387 2761 6788
Fragments arranged by size:
6788 2761 387
PspOMI G'GGCC_C
Cuts at: 0 298 4030 4527 4860 5004 5857 6464 6848
Size: 298 3732 497 333 144 853 607 384
Cuts at: 6848 7016 9005 9804 9850 9936
Size: 168 1989 799 46 86
Fragments arranged by size:
3732 1989 853 799 607 497 384 333
298 168 144 86 46
PspXI vC'TCGA_Gb
Cuts at: 0 3886 9936
Size: 3886 6050
PstI C_TGCA'G
Cuts at: 0 1361 1443 2945 3273 3650 4029 4058 4925
Size: 1361 82 1502 328 377 379 29 867
Cuts at: 4925 5663 7543 7674 7812 8897 9936
Size: 738 1880 131 138 1085 1039
Fragments arranged by size:
1880 1502 1361 1085 1039 867 738 379
377 328 138 131 82 29
PvuII CAG'CTG
Cuts at: 0 1362 1429 1444 2308 3205 3651 5234 6326
Size: 1362 67 15 864 897 446 1583 1092
Cuts at: 6326 7797 9130 9213 9609 9666 9936
Size: 1471 1333 83 396 57 270
Fragments arranged by size:
1583 1471 1362 1333 1092 897 864 446
396 270 83 67 57 15
SacI G_AGCT'C
Cuts at: 0 4307 6557 7760 9592 9936
Size: 4307 2250 1203 1832 344
Fragments arranged by size:
4307 2250 1832 1203 344
SacII CC_GC'GG
Cuts at: 0 1028 6205 7917 9936
Size: 1028 5177 1712 2019
Fragments arranged by size:
5177 2019 1712 1028
SalI G'TCGA_C
Cuts at: 0 42 9936
Size: 42 9894
SanDI GG'GwC_CC
Cuts at: 0 126 483 7449 7520 9299 9546 9936
Size: 126 357 6966 71 1779 247 390
Fragments arranged by size:
6966 1779 390 357 247 126 71
SapI GCTCTTCn'nnn_
Cuts at: 0 4660 7864 8314 9936
Size: 4660 3204 450 1622
Fragments arranged by size:
4660 3204 1622 450
ScaI AGT'ACT
Cuts at: 0 2062 9936
Size: 2062 7874
SexAI A'CCwGG_T
Cuts at: 0 2768 5516 5574 9614 9936
Size: 2768 2748 58 4040 322
Fragments arranged by size:
4040 2768 2748 322 58
SfcI C'TryA_G
Cuts at: 0 1357 1439 2941 3269 3646 4025 4054 4921
Size: 1357 82 1502 328 377 379 29 867
Cuts at: 4921 5659 7408 7539 7670 7808 8893 9936
Size: 738 1749 131 131 138 1085 1043
Fragments arranged by size:
1749 1502 1357 1085 1043 867 738 379
377 328 138 131 131 82 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5588 8288 9936
Size: 5588 2700 1648
Fragments arranged by size:
5588 2700 1648
SfoI GGC'GCC
Cuts at: 0 406 815 1075 4518 4669 7920 8472 8917
Size: 406 409 260 3443 151 3251 552 445
Cuts at: 8917 9396 9936
Size: 479 540
Fragments arranged by size:
3443 3251 552 540 479 445 409 406
260 151
SgrAI Cr'CCGG_yG
Cuts at: 0 1060 9936
Size: 1060 8876
SmaI CCC'GGG
Cuts at: 0 488 835 873 887 1566 6460 7012 9487
Size: 488 347 38 14 679 4894 552 2475
Cuts at: 9487 9936
Size: 449
Fragments arranged by size:
4894 2475 679 552 488 449 347 38
14
SmlI C'TyrA_G
Cuts at: 0 2183 3835 3886 4782 7167 7699 9446 9540
Size: 2183 1652 51 896 2385 532 1747 94
Cuts at: 9540 9936
Size: 396
Fragments arranged by size:
2385 2183 1747 1652 896 532 396 94
51
SphI G_CATG'C
Cuts at: 0 828 7890 9936
Size: 828 7062 2046
Fragments arranged by size:
7062 2046 828
StuI AGG'CCT
Cuts at: 0 5696 9936
Size: 5696 4240
StyI C'CwwG_G
Cuts at: 0 154 1994 2037 2190 4845 4868 5144 5356
Size: 154 1840 43 153 2655 23 276 212
Cuts at: 5356 5639 6009 6306 6605 6947 8696 9107 9398
Size: 283 370 297 299 342 1749 411 291
Cuts at: 9398 9648 9705 9800 9936
Size: 250 57 95 136
Fragments arranged by size:
2655 1840 1749 411 370 342 299 297
291 283 276 250 212 154 153 136
95 57 43 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2431 2650 4315 6022 6460 7096 7642 8827
Size: 2431 219 1665 1707 438 636 546 1185
Cuts at: 8827 9678 9936
Size: 851 258
Fragments arranged by size:
2431 1707 1665 1185 851 636 546 438
258 219
TatI w'GTAC_w
Cuts at: 0 980 2060 2990 9936
Size: 980 1080 930 6946
Fragments arranged by size:
6946 1080 980 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3182 3214 3423 3455 6061 6093 9453 9485
Size: 3182 32 209 32 2606 32 3360 32
Cuts at: 9485 9936
Size: 451
Fragments arranged by size:
3360 3182 2606 451 209 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 8560 9688 9936
Size: 8560 1128 248
Fragments arranged by size:
8560 1128 248
XbaI T'CTAG_A
Cuts at: 0 9202 9936
Size: 9202 734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 1251 1497 1917 4246 4875 4989 5299
Size: 568 683 246 420 2329 629 114 310
Cuts at: 5299 6089 6427 7170 8617 8659 9723 9936
Size: 790 338 743 1447 42 1064 213
Fragments arranged by size:
2329 1447 1064 790 743 683 629 568
420 338 310 246 213 114 42
XhoI C'TCGA_G
Cuts at: 0 2183 3886 9446 9936
Size: 2183 1703 5560 490
Fragments arranged by size:
5560 2183 1703 490
XmaI C'CCGG_G
Cuts at: 0 486 833 871 885 1564 6458 7010 9485
Size: 486 347 38 14 679 4894 552 2475
Cuts at: 9485 9936
Size: 451
Fragments arranged by size:
4894 2475 679 552 486 451 347 38
14
ZraI GAC'GTC
Cuts at: 0 3927 9936
Size: 3927 6009
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SalI SanDI SapI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AclI AflII ApoI AscI AseI AsiSI BaeI BsaBI
BsrGI BstBI BstZ17I ClaI DraI EcoRI EcoRV FspAI
HindIII HpaI MfeI NdeI PacI PmeI PsiI PsrI
PvuI RsrII SbfI SgfI SnaBI SpeI SrfI SspI
SwaI XmnI