Restriction map for
pFN21AE2301
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# NO CUT ENZYMES ( 41 enzymes) #
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AatII AscI AseI AsnI AvrII
BamHI BbeI BglI BlnI BsiWI
BsmBI BssHII BstBI BstEII BstPI
DrdI EcoO65I FseI HindIII HpaI
KasI MfeI MluI MunI NarI
NdeI NheI NruI NspV PacI
PmeI PshBI PvuI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SwaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 5881 GGTACC G^GTACC
AccIII 5451 TCCGGA T^CCGGA
AclI 3881 AACGTT AA^CGTT
AgeI 2879 ACCGGT A^CCGGT
Asp718I 5881 GGTACC G^GTACC
AspI 4185 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3848 TGCGCA TGC^GCA
BalI 716 TGGCCA TGG^CCA
BbsI 4074 GTCTTC GAAGAC(2/6)
BcgI 4850 CGAAGAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4097 TGATCA T^GATCA
BpuAI 4074 GTCTTC GAAGAC(2/6)
BsaBI 4689 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5451 TCCGGA T^CCGGA
BspDI 4972 ATCGAT AT^CGAT
BspEI 5451 TCCGGA T^CCGGA
Bst1107I 1839 GTATAC GTA^TAC
BstZ17I 1839 GTATAC GTA^TAC
ClaI 4972 ATCGAT AT^CGAT
CpoI 2881 CGGTCCG CG^G[AT]CCG
DraI 2952 TTTAAA TTT^AAA
EagI 46 CGGCCG C^GGCCG
EclXI 46 CGGCCG C^GGCCG
Eco52I 46 CGGCCG C^GGCCG
EcoRI 1774 GAATTC G^AATTC
EcoT22I 4585 ATGCAT ATGCA^T
FbaI 4097 TGATCA T^GATCA
FspI 3848 TGCGCA TGC^GCA
KpnI 5881 GGTACC GGTAC^C
KspI 49 CCGCGG CCGC^GG
MamI 4689 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 716 TGGCCA TGG^CCA
MroI 5451 TCCGGA T^CCGGA
MscI 716 TGGCCA TGG^CCA
NaeI 884 GCCGGC GCC^GGC
NcoI 5885 CCATGG C^CATGG
NgoMIV 884 GCCGGC G^CCGGC
NotI 45 GCGGCCGC GC^GGCCGC
NsiI 4585 ATGCAT ATGCA^T
PaeR7I 5144 CTCGAG C^TCGAG
PflFI 4185 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2879 ACCGGT A^CCGGT
Psp1406I 3881 AACGTT AA^CGTT
RsrII 2881 CGGTCCG CG^G[AT]CCG
SacII 49 CCGCGG CCGC^GG
SalI 4708 GTCGAC G^TCGAC
SmaI 5415 CCCGGG CCC^GGG
SspI 2642 AATATT AAT^ATT
Tth111I 4185 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5144 CTCGAG C^TCGAG
XmaCI 5415 CCCGGG C^CCGGG
XmaI 5415 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AflII 3286 CTTAAG C^TTAAG
3889 CTTAAG
AhdI 3813 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4711 GACATGCAGTC
Alw44I 4519 GTGCAC G^TGCAC
5995 GTGCAC
AocI 76 CCTCAGG CC^T[ACGT]AGG
5187 CCTGAGG
Aor51HI 2903 AGCGCT AGC^GCT
4262 AGCGCT
ApaLI 4519 GTGCAC G^TGCAC
5995 GTGCAC
AspEI 3813 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4711 GACATGCAGTC
BbrPI 2191 CACGTG CAC^GTG
4947 CACGTG
BfrI 3286 CTTAAG C^TTAAG
3889 CTTAAG
BsaWI 2879 ACCGGT [AT]^CCGG[AT]
5451 TCCGGA
BsiEI 46 CGGCCG CG[GA][CT]^CG
3609 CGACCG
BsmI 740 GAATGC GAATGC(1/-1)
4037 GCATTC
BssSI 2568 CACGAG CACGAG(-5/-1)
5558 CTCGTG
Bsu36I 76 CCTCAGG CC^T[ACGT]AGG
5187 CCTGAGG
DraIII 3260 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4662 CACGGAGTG
Eam1105I 3813 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4711 GACATGCAGTC
Eco47III 2903 AGCGCT AGC^GCT
4262 AGCGCT
Eco81I 76 CCTCAGG CC^T[ACGT]AGG
5187 CCTGAGG
EcoNI 2814 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3583 CCTACAGTAGG
EcoRV 2329 GATATC GAT^ATC
2482 GATATC
PmaCI 2191 CACGTG CAC^GTG
4947 CACGTG
PmlI 2191 CACGTG CAC^GTG
4947 CACGTG
PshAI 262 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5646 GACTGGCGTC
SacI 2971 GAGCTC GAGCT^C
4729 GAGCTC
SexAI 2525 ACCAGGT A^CC[AT]GGT
4613 ACCTGGT
StuI 3290 AGGCCT AGG^CCT
5517 AGGCCT
XbaI 3075 TCTAGA T^CTAGA
4257 TCTAGA
XcmI 4673 CCATGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5466 CCACCCAAACCCTGG
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# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BciVI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I SbfI ScaI ScrFI
SfaNI SfcI SmlI Sse8387I SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI