################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AflII AflIII AhdI AscI Asp700I AspEI AspI BbeI BfrI BglI BglII BlpI Bpu1102I BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII BstAPI BstBI BstEII BstPI CelII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2254 GGTACC G^GTACC AccII 4396 CGCG CG^CG AclI 1295 AACGTT AA^CGTT AgeI 4755 ACCGGT A^CCGGT AocI 3628 CCTCAGG CC^T[ACGT]AGG AseI 6123 ATTAAT AT^TAAT AsnI 6123 ATTAAT AT^TAAT Asp718I 2254 GGTACC G^GTACC AvaI 2776 CCCGAG C^[CT]CG[GA]G AviII 4506 TGCGCA TGC^GCA AvrII 1005 CCTAGG C^CTAGG BalI 5712 TGGCCA TGG^CCA BbrPI 6537 CACGTG CAC^GTG BbvCI 1681 CCTCAGC CCTCAGC(-5/-2) BcgI 1899 GCATAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5738 GTATCC GTATCC(6/5) BclI 572 TGATCA T^GATCA BlnI 1005 CCTAGG C^CTAGG BsaBI 3357 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4755 ACCGGT [AT]^CCGG[AT] BsoBI 2776 CCCGAG C^[CT]CG[GA]G BssSI 1688 CACGAG CACGAG(-5/-1) Bst1107I 4330 GTATAC GTA^TAC BstUI 4396 CGCG CG^CG BstZ17I 4330 GTATAC GTA^TAC Bsu36I 3628 CCTCAGG CC^T[ACGT]AGG Eco81I 3628 CCTCAGG CC^T[ACGT]AGG FbaI 572 TGATCA T^GATCA FspI 4506 TGCGCA TGC^GCA HindIII 1285 AAGCTT A^AGCTT KpnI 2254 GGTACC GGTAC^C MamI 3357 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 5712 TGGCCA TGG^CCA MscI 5712 TGGCCA TGG^CCA MvnI 4396 CGCG CG^CG NheI 1216 GCTAGC G^CTAGC PinAI 4755 ACCGGT A^CCGGT PmaCI 6537 CACGTG CAC^GTG PmlI 6537 CACGTG CAC^GTG PshBI 6123 ATTAAT AT^TAAT Psp1406I 1295 AACGTT AA^CGTT ScaI 624 AGTACT AGT^ACT SgrAI 2288 CGCCGGTG C[GA]^CCGG[CT]G SphI 3947 GCATGC GCATG^C SspI 5569 AATATT AAT^ATT XbaI 6247 TCTAGA T^CTAGA XcmI 909 CCACATTGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1776 GTCTAC GT^[AC][GT]AC 4330 GTATAC AcyI 215 GGCGTC G[GA]^CG[CT]C 5705 GGCGTC Alw44I 2803 GTGCAC G^TGCAC 3910 GTGCAC Aor51HI 3076 AGCGCT AGC^GCT 5507 AGCGCT ApaI 1309 GGGCCC GGGCC^C 4636 GGGCCC ApaLI 2803 GTGCAC G^TGCAC 3910 GTGCAC BamHI 1245 GGATCC G^GATCC 2836 GGATCC BsaHI 215 GGCGTC G[GA]^CG[CT]C 5705 GGCGTC BsmBI 1909 CGTCTC CGTCTC(1/5) 5440 GAGACG Bsp1407I 5977 TGTACA T^GTACA 6605 TGTACA BspHI 297 TCATGA T^CATGA 6617 TCATGA BsrBI 4464 GAGCGG CCGCTC(-3/-3) 5362 GAGCGG BsrFI 2289 GCCGGT [GA]^CCGG[CT] 4755 ACCGGT BsrGI 5977 TGTACA T^GTACA 6605 TGTACA Cfr10I 2289 GCCGGT [GA]^CCGG[CT] 4755 ACCGGT DraIII 1916 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 6583 CACTTTGTG DrdI 1789 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5699 GACTTGGGCGTC Eco47III 3076 AGCGCT AGC^GCT 5507 AGCGCT EcoNI 2388 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3624 CCTTCCTCAGG EcoT22I 1149 ATGCAT ATGCA^T 1491 ATGCAT FauI 3289 CCCGC CCCGC(4/6) 6453 GCGGG Hin1I 215 GGCGTC G[GA]^CG[CT]C 5705 GGCGTC NsiI 1149 ATGCAT ATGCA^T 1491 ATGCAT RcaI 297 TCATGA T^CATGA 6617 TCATGA SacI 1948 GAGCTC GAGCT^C 4920 GAGCTC SexAI 1043 ACCTGGT A^CC[AT]GGT 2274 ACCAGGT SspBI 5977 TGTACA T^GTACA 6605 TGTACA StuI 1939 AGGCCT AGG^CCT 4283 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII