(Circular) (Six-base) MAPSORT of: pFN21AE2303.seq Check: 8395 from: 1 to: 11335 pFN21AE2303 11335 bp With 182 enzymes: SgfI * November 26, 2010 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2623 4492 2623 Size: 1869 9466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9145 278 Size: 53 83 186 936 7609 2468 Fragments arranged by size: 7609 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3782 6336 8678 1937 Size: 1845 2554 2342 4594 Fragments arranged by size: 4594 2554 2342 1845 Acc65I G'GTAC_C Cuts at: 4259 8656 4259 Size: 4397 6938 AclI AA'CG_TT Cuts at: 1563 3301 9462 9835 1563 Size: 1738 6161 373 3063 Fragments arranged by size: 6161 3063 1738 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 4883 4916 6301 6801 9398 10782 11050 Size: 1200 2051 33 1385 500 2597 1384 268 Cuts at: 11050 1632 Size: 1917 Fragments arranged by size: 2597 2051 1917 1385 1384 1200 500 268 33 AfeI AGC'GCT Cuts at: 5083 7514 5083 Size: 2431 8904 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11138 AflIII A'CryG_T Cuts at: 1203 10234 11211 1203 Size: 9031 977 1327 Fragments arranged by size: 9031 1327 977 AgeI A'CCGG_T Cuts at: 6760 10166 6760 Size: 3406 7929 AhdI GACnn_n'nnGTC Cuts at: 10064 10064 Size: 11335 AleI CACnn'nnGTG Cuts at: 1166 2543 4295 5066 1166 Size: 1377 1752 771 7435 Fragments arranged by size: 7435 1752 1377 771 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3054 4025 4380 1184 Size: 1870 971 355 8139 Fragments arranged by size: 8139 1870 971 355 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2991 3023 8132 8164 2991 Size: 32 5109 32 6162 Fragments arranged by size: 6162 5109 32 32 AlwNI CAG_nnn'CTG Cuts at: 6042 6427 7898 8287 9078 10650 6042 Size: 385 1471 389 791 1572 6727 Fragments arranged by size: 6727 1572 1471 791 389 385 ApaI G_GGCC'C Cuts at: 3318 6645 3318 Size: 3327 8008 ApaLI G'TGCA_C Cuts at: 4808 5915 9392 10548 4808 Size: 1107 3477 1156 5595 Fragments arranged by size: 5595 3477 1156 1107 ApoI r'AATT_y Cuts at: 1493 4739 5572 5725 6984 7162 8449 8644 8870 Size: 3246 833 153 1259 178 1287 195 226 Cuts at: 8870 9022 11131 1493 Size: 152 2109 1697 Fragments arranged by size: 3246 2109 1697 1287 1259 833 226 195 178 153 152 AseI AT'TA_AT Cuts at: 160 1032 8129 9889 160 Size: 872 7097 1760 1606 Fragments arranged by size: 7097 1760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11335 AvaI C'yCGr_G Cuts at: 1870 1943 4781 8660 1870 Size: 73 2838 3879 4545 Fragments arranged by size: 4545 3879 2838 73 AvrII C'CTAG_G Cuts at: 3010 3010 Size: 11335 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5467 5500 5467 Size: 33 11302 BamHI G'GATC_C Cuts at: 3250 4841 8665 9032 3250 Size: 1591 3824 367 5553 Fragments arranged by size: 5553 3824 1591 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2400 4259 4297 4414 8656 Size: 325 217 627 613 1859 38 117 4242 Cuts at: 8656 10111 618 Size: 1455 1842 Fragments arranged by size: 4242 1859 1842 1455 627 613 325 217 117 38 BanII G_rGCy'C Cuts at: 1391 3318 3957 4706 5295 6645 6929 7869 7913 Size: 1927 639 749 589 1350 284 940 44 Cuts at: 7913 8210 8654 1391 Size: 297 444 4072 Fragments arranged by size: 4072 1927 1350 940 749 639 589 444 297 284 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 2778 4530 8237 928 Size: 971 702 177 1752 3707 4026 Fragments arranged by size: 4026 3707 1752 971 702 177 BbvCI CC'TCA_GC Cuts at: 3687 3687 Size: 11335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3893 3927 9526 9560 1903 Size: 34 1956 34 5599 34 3678 Fragments arranged by size: 5599 3678 1956 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7754 9227 10437 7754 Size: 1473 1210 8652 Fragments arranged by size: 8652 1473 1210 BclI T'GATC_A Cuts at: 1548 2577 1548 Size: 1029 10306 BfrBI ATG'CAT Cuts at: 3156 3498 3156 Size: 342 10993 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9946 136 Size: 107 122 71 631 605 8274 1525 Fragments arranged by size: 8274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 11330 11330 Size: 11335 BlpI GC'TnA_GC Cuts at: 8752 8752 Size: 11335 Bme1580I G_kGCm'C Cuts at: 1790 3318 4812 5198 5919 6645 8378 9396 10552 Size: 1528 1494 386 721 726 1733 1018 1156 Cuts at: 10552 1790 Size: 2573 Fragments arranged by size: 2573 1733 1528 1494 1156 1018 726 721 386 BmgBI CAC'GTC Cuts at: 1318 8490 1318 Size: 7172 4163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 3166 3918 Size: 416 53 83 399 24 615 1134 752 Cuts at: 3918 4063 6867 8145 8162 8329 9066 10024 442 Size: 145 2804 1278 17 167 737 958 1753 Fragments arranged by size: 2804 1753 1278 1134 958 752 737 615 416 399 167 145 83 53 24 17 BmtI G_CTAG'C Cuts at: 1056 3225 1056 Size: 2169 9166 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4009 6948 7018 7096 4009 Size: 2939 70 78 8248 Fragments arranged by size: 8248 2939 78 70 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2928 2979 3755 3933 3941 4140 4369 Size: 827 862 51 776 178 8 199 229 Cuts at: 4369 5109 5675 5800 7011 7160 7705 8013 9995 Size: 740 566 125 1211 149 545 308 1982 Cuts at: 9995 1239 Size: 2579 Fragments arranged by size: 2579 1982 1211 862 827 776 740 566 545 308 229 199 178 149 125 51 8 Bpu10I CC'TnA_GC Cuts at: 3687 6135 6646 7149 7365 3687 Size: 2448 511 503 216 7657 Fragments arranged by size: 7657 2448 511 503 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6346 7340 7772 7953 9460 10325 10623 6346 Size: 994 432 181 1507 865 298 7058 Fragments arranged by size: 7058 1507 994 865 432 298 181 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3442 6769 7553 9998 882 Size: 617 1943 3327 784 2445 2219 Fragments arranged by size: 3327 2445 2219 1943 784 617 BsaAI yAC'GTr Cuts at: 493 1204 6386 7062 7619 8544 9126 493 Size: 711 5182 676 557 925 582 2702 Fragments arranged by size: 5182 2702 925 711 676 582 557 BsaBI GATnn'nnATC Cuts at: 5366 11329 5366 Size: 5963 5372 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2221 7711 9142 9524 Size: 53 83 186 936 688 5490 1431 382 Cuts at: 9524 10159 275 Size: 635 1451 Fragments arranged by size: 5490 1451 1431 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6760 9768 10166 10440 10587 10998 6760 Size: 3008 398 274 147 411 7097 Fragments arranged by size: 7097 3008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3753 3783 8221 8251 1178 Size: 30 165 30 2350 30 4438 30 4262 Fragments arranged by size: 4438 4262 2350 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 3196 3538 5672 5675 5678 5950 6491 Size: 1414 596 342 2134 3 3 272 541 Cuts at: 6491 7088 7615 8282 1186 Size: 597 527 667 4239 Fragments arranged by size: 4239 2134 1414 667 597 596 541 527 342 272 3 3 BseYI C'CCAG_C Cuts at: 2152 2556 2692 5089 6638 6673 6851 7373 7438 Size: 404 136 2397 1549 35 178 522 65 Cuts at: 7438 10538 11034 2152 Size: 3100 496 2453 Fragments arranged by size: 3100 2453 2397 1549 522 496 404 178 136 65 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 3327 3375 6083 7297 7516 7599 Size: 573 865 726 48 2708 1214 219 83 Cuts at: 7599 1163 Size: 4899 Fragments arranged by size: 4899 2708 1214 865 726 573 219 83 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8785 9546 10574 1540 Size: 275 186 6784 761 1028 2301 Fragments arranged by size: 6784 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3957 4004 4812 5919 6929 7482 8601 9396 9481 Size: 47 808 1107 1010 553 1119 795 85 Cuts at: 9481 10552 3957 Size: 1071 4740 Fragments arranged by size: 4740 1119 1107 1071 1010 808 795 553 85 47 BsmI GAATG_Cn' Cuts at: 3158 5831 6640 8846 8939 3158 Size: 2673 809 2206 93 5554 Fragments arranged by size: 5554 2673 2206 809 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3920 7439 3920 Size: 3519 7816 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3318 3957 4004 4706 4812 5198 5295 Size: 399 1528 639 47 702 106 386 97 Cuts at: 5295 5919 6645 6929 7482 7869 7913 8210 8378 Size: 624 726 284 553 387 44 297 168 Cuts at: 8378 8601 8654 9396 9481 10552 1391 Size: 223 53 742 85 1071 2174 Fragments arranged by size: 2174 1528 1071 742 726 702 639 624 553 399 387 386 297 284 223 168 106 97 85 53 47 44 BspEI T'CCGG_A Cuts at: 10998 10998 Size: 11335 BspHI T'CATG_A Cuts at: 2302 8622 9224 10954 2302 Size: 6320 602 1730 2683 Fragments arranged by size: 6320 2683 1730 602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2302 2623 4492 7571 8690 844 Size: 1370 88 321 1869 3079 1119 3489 Fragments arranged by size: 3489 3079 1869 1370 1119 321 88 BsrBI CCG'CTC Cuts at: 1394 1823 6471 7369 9222 1394 Size: 429 4648 898 1853 3507 Fragments arranged by size: 4648 3507 1853 898 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2916 4007 4097 5941 9830 10004 65 Size: 1183 1668 1091 90 1844 3889 174 1396 Fragments arranged by size: 3889 1844 1668 1396 1183 1091 174 90 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 6760 9979 10166 1700 Size: 110 2484 2466 3219 187 2869 Fragments arranged by size: 3219 2869 2484 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 7982 8610 96 Size: 7886 628 2821 Fragments arranged by size: 7886 2821 628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11335 BssSI C'ACGA_G Cuts at: 3693 9395 10407 3693 Size: 5702 1012 4621 Fragments arranged by size: 5702 4621 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 11335 BstBI TT'CG_AA Cuts at: 10139 10139 Size: 11335 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11335 BstXI CCAn_nnnn'nTGG Cuts at: 2287 3049 6078 6160 8023 2287 Size: 762 3029 82 1863 5599 Fragments arranged by size: 5599 3029 1863 762 82 BstYI r'GATC_y Cuts at: 1972 3250 4841 5029 7299 8665 9032 9417 9434 Size: 1278 1591 188 2270 1366 367 385 17 Cuts at: 9434 10886 10972 10984 11330 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2270 1977 1591 1452 1366 1278 385 367 346 188 86 17 12 BstZ17I GTA'TAC Cuts at: 6337 6337 Size: 11335 Bsu36I CC'TnA_GG Cuts at: 5634 5634 Size: 11335 BtgI C'CryG_G Cuts at: 513 1065 6990 7251 7543 7720 7860 513 Size: 552 5925 261 292 177 140 3988 Fragments arranged by size: 5925 3988 552 292 261 177 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10596 Fragments arranged by size: 10596 683 56 BtsI GCAGTG_nn' Cuts at: 4002 5196 6688 7286 8851 9645 9665 4002 Size: 1194 1492 598 1565 794 20 5672 Fragments arranged by size: 5672 1565 1492 1194 794 598 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4596 4631 529 Size: 35 4032 35 7233 Fragments arranged by size: 7233 4032 35 35 DraI TTT'AAA Cuts at: 3297 4045 5374 8639 3297 Size: 748 1329 3265 5993 Fragments arranged by size: 5993 3265 1329 748 DraIII CAC_nnn'GTG Cuts at: 3926 8593 3926 Size: 4667 6668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3800 7710 10342 809 Size: 507 2484 3910 2632 1802 Fragments arranged by size: 3910 2632 2484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3483 3591 7717 Size: 54 1425 50 275 18 1653 108 4126 Cuts at: 7717 7921 8553 8782 9671 11300 8 Size: 204 632 229 889 1629 43 Fragments arranged by size: 4126 1653 1629 1425 889 632 275 229 204 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8782 1537 Size: 275 6970 4090 Fragments arranged by size: 6970 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 3207 4543 5543 5769 6817 6934 7487 Size: 1022 832 1336 1000 226 1048 117 553 Cuts at: 7487 8167 8387 9265 1353 Size: 680 220 878 3423 Fragments arranged by size: 3423 1336 1048 1022 1000 878 832 680 553 226 220 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5447 9922 10296 10442 387 Size: 1064 138 3858 4475 374 146 1280 Fragments arranged by size: 4475 3858 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3955 6927 3955 Size: 2972 8363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2832 2928 2979 3755 3933 3941 Size: 393 434 766 96 51 776 178 8 Cuts at: 3941 4140 4369 4883 4916 5109 5675 5800 6301 Size: 199 229 514 33 193 566 125 501 Cuts at: 6301 6801 7011 7160 7705 8013 9398 9995 10782 Size: 500 210 149 545 308 1385 597 787 Cuts at: 10782 11050 1239 Size: 268 1524 Fragments arranged by size: 1524 1385 787 776 766 597 566 545 514 501 500 434 393 308 268 229 210 199 193 178 149 125 96 51 33 8 EcoNI CCTnn'n_nnAGG Cuts at: 4397 5633 4397 Size: 1236 10099 EcoO109I rG'GnC_Cy Cuts at: 2953 3474 3870 5169 6039 6642 7875 2953 Size: 521 396 1299 870 603 1233 6413 Fragments arranged by size: 6413 1299 1233 870 603 521 396 EcoRI G'AATT_C Cuts at: 4739 5572 6984 8449 8644 9022 4739 Size: 833 1412 1465 195 378 7052 Fragments arranged by size: 7052 1465 1412 833 378 195 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3469 3469 Size: 11335 FspI TGC'GCA Cuts at: 6513 9841 6513 Size: 3328 8007 HaeII r_GCGC'y Cuts at: 1678 5085 5605 7516 10482 1678 Size: 3407 520 1911 2966 2531 Fragments arranged by size: 3407 2966 2531 1911 520 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2371 2403 2477 Size: 32 135 32 470 32 297 32 74 Cuts at: 2477 2509 3070 3102 3710 3742 3753 3785 3810 Size: 32 561 32 608 32 11 32 25 Cuts at: 3810 3842 5228 5260 7680 7712 9976 10008 10050 Size: 32 1386 32 2420 32 2264 32 42 Cuts at: 10050 10082 1373 Size: 32 2626 Fragments arranged by size: 2626 2420 2264 1386 608 561 470 297 135 74 42 32 32 32 32 32 32 32 32 32 32 32 32 32 25 11 HincII GTy'rAC Cuts at: 669 1657 1938 2476 3195 8086 8679 8925 669 Size: 988 281 538 719 4891 593 246 3079 Fragments arranged by size: 4891 3079 988 719 593 538 281 246 HindIII A'AGCT_T Cuts at: 748 3290 748 Size: 2542 8793 HpaI GTT'AAC Cuts at: 8925 8925 Size: 11335 KpnI G_GTAC'C Cuts at: 4263 8660 4263 Size: 4397 6938 MfeI C'AATT_G Cuts at: 8934 8934 Size: 11335 MluI A'CGCG_T Cuts at: 11211 11211 Size: 11335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 2470 3224 3278 5535 5820 6210 6817 Size: 780 385 754 54 2257 285 390 607 Cuts at: 6817 8207 8472 10449 10633 1305 Size: 1390 265 1977 184 2007 Fragments arranged by size: 2257 2007 1977 1390 780 754 607 390 385 285 265 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 7719 10 Size: 54 1425 343 5887 3626 Fragments arranged by size: 5887 3626 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 4295 5066 5934 5951 6252 6401 Size: 648 1377 1752 771 868 17 301 149 Cuts at: 6401 7043 7130 7346 7994 8021 8129 9293 9652 Size: 642 87 216 648 27 108 1164 359 Cuts at: 9652 9811 518 Size: 159 2042 Fragments arranged by size: 2042 1752 1377 1164 868 771 648 648 642 359 301 216 159 149 108 87 27 17 MspA1I CmG'CkG Cuts at: 1606 2190 3270 5394 5481 6677 7031 8458 8751 Size: 584 1080 2124 87 1196 354 1427 293 Cuts at: 8751 9428 10576 10821 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2124 2120 1427 1196 1148 1080 677 584 354 293 245 87 NcoI C'CATG_G Cuts at: 513 1065 7251 7543 7720 7860 513 Size: 552 6186 292 177 140 3988 Fragments arranged by size: 6186 3988 552 292 177 140 NdeI CA'TA_TG Cuts at: 387 3129 4804 6238 7155 387 Size: 2742 1675 1434 917 4567 Fragments arranged by size: 4567 2742 1675 1434 917 NheI G'CTAG_C Cuts at: 1052 3221 1052 Size: 2169 9166 NotI GC'GGCC_GC Cuts at: 8782 8782 Size: 11335 NsiI A_TGCA'T Cuts at: 3158 3500 3158 Size: 342 10993 NspI r_CATG'y Cuts at: 1132 1229 5956 6062 7201 7945 8693 10238 1132 Size: 97 4727 106 1139 744 748 1545 2229 Fragments arranged by size: 4727 2229 1545 1139 748 744 106 97 PasI CC'CwG_GG Cuts at: 1348 6917 7870 1348 Size: 5569 953 4813 Fragments arranged by size: 5569 4813 953 PciI A'CATG_T Cuts at: 10234 10234 Size: 11335 PflMI CCAn_nnn'nTGG Cuts at: 1108 5971 6097 8593 1108 Size: 4863 126 2496 3850 Fragments arranged by size: 4863 3850 2496 126 PfoI T'CCnGG_A Cuts at: 5730 5730 Size: 11335 PmeI GTTT'AAAC Cuts at: 8639 8639 Size: 11335 PmlI CAC'GTG Cuts at: 8544 8544 Size: 11335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4542 4574 9404 9436 10917 10949 4542 Size: 32 4830 32 1481 32 4928 Fragments arranged by size: 4928 4830 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2953 5169 6039 7875 2953 Size: 2216 870 1836 6413 Fragments arranged by size: 6413 2216 1836 870 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11335 PsiI TTA'TAA Cuts at: 4653 8905 4653 Size: 4252 7083 PspOMI G'GGCC_C Cuts at: 3314 6641 3314 Size: 3327 8008 PstI C_TGCA'G Cuts at: 830 2313 4513 4975 5596 5997 6558 6727 7009 Size: 1483 2200 462 621 401 561 169 282 Cuts at: 7009 7072 7206 7336 8687 830 Size: 63 134 130 1351 3478 Fragments arranged by size: 3478 2200 1483 1351 621 561 462 401 282 169 134 130 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11335 PvuII CAG'CTG Cuts at: 3270 5394 6677 7031 3270 Size: 2124 1283 354 7574 Fragments arranged by size: 7574 2124 1283 354 SacI G_AGCT'C Cuts at: 3957 6929 3957 Size: 2972 8363 SalI G'TCGA_C Cuts at: 1936 8677 1936 Size: 6741 4594 SapI GCTCTTCn'nnn_ Cuts at: 2375 3207 4543 6934 7487 2375 Size: 832 1336 2391 553 6223 Fragments arranged by size: 6223 2391 1336 832 553 SbfI CC_TGCA'GG Cuts at: 8687 8687 Size: 11335 ScaI AGT'ACT Cuts at: 2631 9583 11295 2631 Size: 6952 1712 2671 Fragments arranged by size: 6952 2671 1712 SexAI A'CCwGG_T Cuts at: 3048 4279 3048 Size: 1231 10104 SfcI C'TryA_G Cuts at: 826 1045 2309 4509 4971 5592 5993 6554 6723 Size: 219 1264 2200 462 621 401 561 169 Cuts at: 6723 7005 7068 7202 7332 7631 7766 8683 9818 Size: 282 63 134 130 299 135 917 1135 Cuts at: 9818 10499 10690 826 Size: 681 191 1471 Fragments arranged by size: 2200 1471 1264 1135 917 681 621 561 462 401 299 282 219 191 169 135 134 130 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11335 SgrAI Cr'CCGG_yG Cuts at: 1700 4294 1700 Size: 2594 8741 SmaI CCC'GGG Cuts at: 1872 8662 1872 Size: 6790 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6325 7355 7787 7968 9439 10340 Size: 197 926 4382 1030 432 181 1471 901 Cuts at: 10340 10602 820 Size: 262 1553 Fragments arranged by size: 4382 1553 1471 1030 926 901 432 262 197 181 SnaBI TAC'GTA Cuts at: 493 9126 493 Size: 8633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11335 SphI G_CATG'C Cuts at: 1132 5956 8693 1132 Size: 4824 2737 3774 Fragments arranged by size: 4824 3774 2737 SspI AAT'ATT Cuts at: 5 52 7576 9259 5 Size: 47 7524 1683 2081 Fragments arranged by size: 7524 2081 1683 47 StuI AGG'CCT Cuts at: 1517 3946 6290 1517 Size: 2429 2344 6562 Fragments arranged by size: 6562 2429 2344 StyI C'CwwG_G Cuts at: 513 1065 3010 4263 6464 7251 7543 7720 7750 Size: 552 1945 1253 2201 787 292 177 30 Cuts at: 7750 7860 8293 8774 513 Size: 110 433 481 3074 Fragments arranged by size: 3074 2201 1945 1253 787 552 481 433 292 177 110 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 2279 3718 3937 4542 5683 8573 Size: 1255 434 265 1439 219 605 1141 2890 Cuts at: 8573 9362 9379 9532 9717 10162 325 Size: 789 17 153 185 445 1498 Fragments arranged by size: 2890 1498 1439 1255 1141 789 605 445 434 265 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2629 7194 7266 Size: 274 80 33 51 1443 652 4565 72 Cuts at: 7266 7982 8520 8610 9581 11293 96 Size: 716 538 90 971 1712 138 Fragments arranged by size: 4565 1712 1443 971 716 652 538 274 138 90 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3038 3070 3714 3746 4071 4103 5614 5646 5712 Size: 32 644 32 325 32 1511 32 66 Cuts at: 5712 5744 6399 6431 7295 7327 8187 8219 8221 Size: 32 655 32 864 32 860 32 2 Cuts at: 8221 8253 8533 8565 11295 11327 3038 Size: 32 280 32 2730 32 3046 Fragments arranged by size: 3046 2730 1511 864 860 655 644 325 280 66 32 32 32 32 32 32 32 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11335 XbaI T'CTAG_A Cuts at: 8252 8671 8252 Size: 419 10916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2921 2921 Size: 11335 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11335 XmaI C'CCGG_G Cuts at: 1870 8660 1870 Size: 6790 4545 XmnI GAAnn'nnTTC Cuts at: 9464 9464 Size: 11335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9143 276 Size: 53 83 186 936 7609 2468 Fragments arranged by size: 7609 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI