(Linear) (Six-base) MAPSORT of: KIEE2303.seq Check: 7459 from: 1 to: 6630 KIEE2303.orf With 182 enzymes: SgfI * November 26, 2010 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 618 2487 6630 Size: 618 1869 4143 Fragments arranged by size: 4143 1869 618 AccI GT'mk_AC Cuts at: 0 1777 4331 6630 Size: 1777 2554 2299 Fragments arranged by size: 2554 2299 1777 Acc65I G'GTAC_C Cuts at: 0 2254 6630 Size: 2254 4376 AclI AA'CG_TT Cuts at: 0 1296 6630 Size: 1296 5334 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 827 2878 2911 4296 4796 6630 Size: 827 2051 33 1385 500 1834 Fragments arranged by size: 2051 1834 1385 827 500 33 AfeI AGC'GCT Cuts at: 0 3078 5509 6630 Size: 3078 2431 1121 Fragments arranged by size: 3078 2431 1121 AgeI A'CCGG_T Cuts at: 0 4755 6630 Size: 4755 1875 AleI CACnn'nnGTG Cuts at: 0 538 2290 3061 6630 Size: 538 1752 771 3569 Fragments arranged by size: 3569 1752 771 538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1049 2020 2375 6630 Size: 1049 971 355 4255 Fragments arranged by size: 4255 1049 971 355 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 986 1018 6127 6159 6630 Size: 986 32 5109 32 471 Fragments arranged by size: 5109 986 471 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 4037 4422 5893 6282 6630 Size: 4037 385 1471 389 348 Fragments arranged by size: 4037 1471 389 385 348 ApaI G_GGCC'C Cuts at: 0 1313 4640 6630 Size: 1313 3327 1990 Fragments arranged by size: 3327 1990 1313 ApaLI G'TGCA_C Cuts at: 0 2803 3910 6630 Size: 2803 1107 2720 Fragments arranged by size: 2803 2720 1107 ApoI r'AATT_y Cuts at: 0 2734 3567 3720 4979 5157 6444 6630 Size: 2734 833 153 1259 178 1287 186 Fragments arranged by size: 2734 1287 1259 833 186 178 153 AseI AT'TA_AT Cuts at: 0 6124 6630 Size: 6124 506 AvaI C'yCGr_G Cuts at: 0 2776 6630 Size: 2776 3854 AvrII C'CTAG_G Cuts at: 0 1005 6630 Size: 1005 5625 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3462 3495 6630 Size: 3462 33 3135 Fragments arranged by size: 3462 3135 33 BamHI G'GATC_C Cuts at: 0 1245 2836 6630 Size: 1245 1591 3794 Fragments arranged by size: 3794 1591 1245 BanI G'GyrC_C Cuts at: 0 395 2254 2292 2409 6630 Size: 395 1859 38 117 4221 Fragments arranged by size: 4221 1859 395 117 38 BanII G_rGCy'C Cuts at: 0 1313 1952 2701 3290 4640 4924 5864 5908 Size: 1313 639 749 589 1350 284 940 44 Cuts at: 5908 6205 6630 Size: 297 425 Fragments arranged by size: 1350 1313 940 749 639 589 425 297 284 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 596 773 2525 6232 6630 Size: 596 177 1752 3707 398 Fragments arranged by size: 3707 1752 596 398 177 BbvCI CC'TCA_GC Cuts at: 0 1682 6630 Size: 1682 4948 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1888 1922 6630 Size: 1888 34 4708 Fragments arranged by size: 4708 1888 34 BciVI GTATCCnnnnn_n' Cuts at: 0 5749 6630 Size: 5749 881 BclI T'GATC_A Cuts at: 0 572 6630 Size: 572 6058 BfrBI ATG'CAT Cuts at: 0 1151 1493 6630 Size: 1151 342 5137 Fragments arranged by size: 5137 1151 342 Bme1580I G_kGCm'C Cuts at: 0 1313 2807 3193 3914 4640 6373 6630 Size: 1313 1494 386 721 726 1733 257 Fragments arranged by size: 1733 1494 1313 726 721 386 257 BmgBI CAC'GTC Cuts at: 0 6485 6630 Size: 6485 145 BmrI ACTGGGnnnn_n' Cuts at: 0 27 1161 1913 2058 4862 6140 6157 6324 Size: 27 1134 752 145 2804 1278 17 167 Cuts at: 6324 6630 Size: 306 Fragments arranged by size: 2804 1278 1134 752 306 167 145 27 17 BmtI G_CTAG'C Cuts at: 0 1220 6630 Size: 1220 5410 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2004 4943 5013 5091 6630 Size: 2004 2939 70 78 1539 Fragments arranged by size: 2939 2004 1539 78 70 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 923 974 1750 1928 1936 2135 2364 Size: 61 862 51 776 178 8 199 229 Cuts at: 2364 3104 3670 3795 5006 5155 5700 6008 6630 Size: 740 566 125 1211 149 545 308 622 Fragments arranged by size: 1211 862 776 740 622 566 545 308 229 199 178 149 125 61 51 8 Bpu10I CC'TnA_GC Cuts at: 0 1682 4130 4641 5144 5360 6630 Size: 1682 2448 511 503 216 1270 Fragments arranged by size: 2448 1682 1270 511 503 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4341 5335 5767 5948 6630 Size: 4341 994 432 181 682 Fragments arranged by size: 4341 994 682 432 181 BsaI GGTCTCn'nnnn_ Cuts at: 0 1437 4764 5548 6630 Size: 1437 3327 784 1082 Fragments arranged by size: 3327 1437 1082 784 BsaAI yAC'GTr Cuts at: 0 4381 5057 5614 6539 6630 Size: 4381 676 557 925 91 Fragments arranged by size: 4381 925 676 557 91 BsaBI GATnn'nnATC Cuts at: 0 3361 6630 Size: 3361 3269 BsaHI Gr'CG_yC Cuts at: 0 216 5706 6630 Size: 216 5490 924 Fragments arranged by size: 5490 924 216 BsaWI w'CCGG_w Cuts at: 0 4755 6630 Size: 4755 1875 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1748 1778 6216 6246 6630 Size: 1748 30 4438 30 384 Fragments arranged by size: 4438 1748 384 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 595 1191 1533 3667 3670 3673 3945 4486 Size: 595 596 342 2134 3 3 272 541 Cuts at: 4486 5083 5610 6277 6630 Size: 597 527 667 353 Fragments arranged by size: 2134 667 597 596 595 541 527 353 342 272 3 3 BseYI C'CCAG_C Cuts at: 0 147 551 687 3084 4633 4668 4846 5368 Size: 147 404 136 2397 1549 35 178 522 Cuts at: 5368 5433 6630 Size: 65 1197 Fragments arranged by size: 2397 1549 1197 522 404 178 147 136 65 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 596 1322 1370 4078 5292 5511 5594 6630 Size: 596 726 48 2708 1214 219 83 1036 Fragments arranged by size: 2708 1214 1036 726 596 219 83 48 BsiHKAI G_wGCw'C Cuts at: 0 1952 1999 2807 3914 4924 5477 6596 6630 Size: 1952 47 808 1107 1010 553 1119 34 Fragments arranged by size: 1952 1119 1107 1010 808 553 47 34 BsmI GAATG_Cn' Cuts at: 0 1153 3826 4635 6630 Size: 1153 2673 809 1995 Fragments arranged by size: 2673 1995 1153 809 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1915 5434 6630 Size: 1915 3519 1196 Fragments arranged by size: 3519 1915 1196 Bsp1286I G_dGCh'C Cuts at: 0 1313 1952 1999 2701 2807 3193 3290 3914 Size: 1313 639 47 702 106 386 97 624 Cuts at: 3914 4640 4924 5477 5864 5908 6205 6373 6596 Size: 726 284 553 387 44 297 168 223 Cuts at: 6596 6630 Size: 34 Fragments arranged by size: 1313 726 702 639 624 553 387 386 297 284 223 168 106 97 47 44 34 BspHI T'CATG_A Cuts at: 0 297 6617 6630 Size: 297 6320 13 Fragments arranged by size: 6320 297 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 209 297 618 2487 5566 6630 Size: 209 88 321 1869 3079 1064 Fragments arranged by size: 3079 1869 1064 321 209 88 BsrBI CCG'CTC Cuts at: 0 4466 5364 6630 Size: 4466 898 1266 Fragments arranged by size: 4466 1266 898 BsrDI GCAATG_nn' Cuts at: 0 911 2002 2092 3936 6630 Size: 911 1091 90 1844 2694 Fragments arranged by size: 2694 1844 1091 911 90 BsrFI r'CCGG_y Cuts at: 0 2289 4755 6630 Size: 2289 2466 1875 Fragments arranged by size: 2466 2289 1875 BsrGI T'GTAC_A Cuts at: 0 5977 6605 6630 Size: 5977 628 25 Fragments arranged by size: 5977 628 25 BssSI C'ACGA_G Cuts at: 0 1688 6630 Size: 1688 4942 BstXI CCAn_nnnn'nTGG Cuts at: 0 282 1044 4073 4155 6018 6630 Size: 282 762 3029 82 1863 612 Fragments arranged by size: 3029 1863 762 612 282 82 BstYI r'GATC_y Cuts at: 0 1245 2836 3024 5294 6630 Size: 1245 1591 188 2270 1336 Fragments arranged by size: 2270 1591 1336 1245 188 BstZ17I GTA'TAC Cuts at: 0 4332 6630 Size: 4332 2298 Bsu36I CC'TnA_GG Cuts at: 0 3629 6630 Size: 3629 3001 BtgI C'CryG_G Cuts at: 0 4985 5246 5538 5715 5855 6630 Size: 4985 261 292 177 140 775 Fragments arranged by size: 4985 775 292 261 177 140 BtsI GCAGTG_nn' Cuts at: 0 1997 3191 4683 5281 6630 Size: 1997 1194 1492 598 1349 Fragments arranged by size: 1997 1492 1349 1194 598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2591 2626 6630 Size: 2591 35 4004 Fragments arranged by size: 4004 2591 35 DraI TTT'AAA Cuts at: 0 1292 2040 3369 6630 Size: 1292 748 1329 3261 Fragments arranged by size: 3261 1329 1292 748 DraIII CAC_nnn'GTG Cuts at: 0 1921 6588 6630 Size: 1921 4667 42 Fragments arranged by size: 4667 1921 42 DrdI GACnn_nn'nnGTC Cuts at: 0 1795 5705 6630 Size: 1795 3910 925 Fragments arranged by size: 3910 1795 925 EaeI y'GGCC_r Cuts at: 0 1478 1586 5712 5916 6548 6630 Size: 1478 108 4126 204 632 82 Fragments arranged by size: 4126 1478 632 204 108 82 EarI CTCTTCn'nnn_ Cuts at: 0 370 1202 2538 3538 3764 4812 4929 5482 Size: 370 832 1336 1000 226 1048 117 553 Cuts at: 5482 6162 6382 6630 Size: 680 220 248 Fragments arranged by size: 1336 1048 1000 832 680 553 370 248 226 220 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3442 6630 Size: 3442 3188 EcoICRI GAG'CTC Cuts at: 0 1950 4922 6630 Size: 1950 2972 1708 Fragments arranged by size: 2972 1950 1708 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 827 923 974 1750 1928 1936 2135 Size: 61 766 96 51 776 178 8 199 Cuts at: 2135 2364 2878 2911 3104 3670 3795 4296 4796 Size: 229 514 33 193 566 125 501 500 Cuts at: 4796 5006 5155 5700 6008 6630 Size: 210 149 545 308 622 Fragments arranged by size: 776 766 622 566 545 514 501 500 308 229 210 199 193 178 149 125 96 61 51 33 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 2392 3628 6630 Size: 2392 1236 3002 Fragments arranged by size: 3002 2392 1236 EcoO109I rG'GnC_Cy Cuts at: 0 948 1469 1865 3164 4034 4637 5870 6630 Size: 948 521 396 1299 870 603 1233 760 Fragments arranged by size: 1299 1233 948 870 760 603 521 396 EcoRI G'AATT_C Cuts at: 0 2734 3567 4979 6444 6630 Size: 2734 833 1412 1465 186 Fragments arranged by size: 2734 1465 1412 833 186 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1464 6630 Size: 1464 5166 FspI TGC'GCA Cuts at: 0 4508 6630 Size: 4508 2122 HaeII r_GCGC'y Cuts at: 0 3080 3600 5511 6630 Size: 3080 520 1911 1119 Fragments arranged by size: 3080 1911 1119 520 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 37 69 366 398 472 504 1065 1097 Size: 37 32 297 32 74 32 561 32 Cuts at: 1097 1705 1737 1748 1780 1805 1837 3223 3255 Size: 608 32 11 32 25 32 1386 32 Cuts at: 3255 5675 5707 6630 Size: 2420 32 923 Fragments arranged by size: 2420 1386 923 608 561 297 74 37 32 32 32 32 32 32 32 32 32 25 11 HincII GTy'rAC Cuts at: 0 471 1190 6081 6630 Size: 471 719 4891 549 Fragments arranged by size: 4891 719 549 471 HindIII A'AGCT_T Cuts at: 0 1285 6630 Size: 1285 5345 KpnI G_GTAC'C Cuts at: 0 2258 6630 Size: 2258 4372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 80 465 1219 1273 3530 3815 4205 4812 Size: 80 385 754 54 2257 285 390 607 Cuts at: 4812 6202 6467 6630 Size: 1390 265 163 Fragments arranged by size: 2257 1390 754 607 390 385 285 265 163 80 54 MscI TGG'CCA Cuts at: 0 5714 6630 Size: 5714 916 MslI CAynn'nnrTG Cuts at: 0 538 2290 3061 3929 3946 4247 4396 5038 Size: 538 1752 771 868 17 301 149 642 Cuts at: 5038 5125 5341 5989 6016 6124 6630 Size: 87 216 648 27 108 506 Fragments arranged by size: 1752 868 771 648 642 538 506 301 216 149 108 87 27 17 MspA1I CmG'CkG Cuts at: 0 185 1265 3389 3476 4672 5026 6453 6630 Size: 185 1080 2124 87 1196 354 1427 177 Fragments arranged by size: 2124 1427 1196 1080 354 185 177 87 NcoI C'CATG_G Cuts at: 0 5246 5538 5715 5855 6630 Size: 5246 292 177 140 775 Fragments arranged by size: 5246 775 292 177 140 NdeI CA'TA_TG Cuts at: 0 1124 2799 4233 5150 6630 Size: 1124 1675 1434 917 1480 Fragments arranged by size: 1675 1480 1434 1124 917 NheI G'CTAG_C Cuts at: 0 1216 6630 Size: 1216 5414 NsiI A_TGCA'T Cuts at: 0 1153 1495 6630 Size: 1153 342 5135 Fragments arranged by size: 5135 1153 342 NspI r_CATG'y Cuts at: 0 3951 4057 5196 5940 6630 Size: 3951 106 1139 744 690 Fragments arranged by size: 3951 1139 744 690 106 PasI CC'CwG_GG Cuts at: 0 4912 5865 6630 Size: 4912 953 765 Fragments arranged by size: 4912 953 765 PflMI CCAn_nnn'nTGG Cuts at: 0 3966 4092 6588 6630 Size: 3966 126 2496 42 Fragments arranged by size: 3966 2496 126 42 PfoI T'CCnGG_A Cuts at: 0 3725 6630 Size: 3725 2905 PmlI CAC'GTG Cuts at: 0 6539 6630 Size: 6539 91 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2537 2569 6630 Size: 2537 32 4061 Fragments arranged by size: 4061 2537 32 PpuMI rG'GwC_Cy Cuts at: 0 948 3164 4034 5870 6630 Size: 948 2216 870 1836 760 Fragments arranged by size: 2216 1836 948 870 760 PsiI TTA'TAA Cuts at: 0 2648 6630 Size: 2648 3982 PspOMI G'GGCC_C Cuts at: 0 1309 4636 6630 Size: 1309 3327 1994 Fragments arranged by size: 3327 1994 1309 PstI C_TGCA'G Cuts at: 0 308 2508 2970 3591 3992 4553 4722 5004 Size: 308 2200 462 621 401 561 169 282 Cuts at: 5004 5067 5201 5331 6630 Size: 63 134 130 1299 Fragments arranged by size: 2200 1299 621 561 462 401 308 282 169 134 130 63 PvuII CAG'CTG Cuts at: 0 1265 3389 4672 5026 6630 Size: 1265 2124 1283 354 1604 Fragments arranged by size: 2124 1604 1283 1265 354 SacI G_AGCT'C Cuts at: 0 1952 4924 6630 Size: 1952 2972 1706 Fragments arranged by size: 2972 1952 1706 SapI GCTCTTCn'nnn_ Cuts at: 0 370 1202 2538 4929 5482 6630 Size: 370 832 1336 2391 553 1148 Fragments arranged by size: 2391 1336 1148 832 553 370 ScaI AGT'ACT Cuts at: 0 626 6630 Size: 626 6004 SexAI A'CCwGG_T Cuts at: 0 1043 2274 6630 Size: 1043 1231 4356 Fragments arranged by size: 4356 1231 1043 SfcI C'TryA_G Cuts at: 0 304 2504 2966 3587 3988 4549 4718 5000 Size: 304 2200 462 621 401 561 169 282 Cuts at: 5000 5063 5197 5327 5626 5761 6630 Size: 63 134 130 299 135 869 Fragments arranged by size: 2200 869 621 561 462 401 304 299 282 169 135 134 130 63 SgrAI Cr'CCGG_yG Cuts at: 0 2289 6630 Size: 2289 4341 SmlI C'TyrA_G Cuts at: 0 4320 5350 5782 5963 6630 Size: 4320 1030 432 181 667 Fragments arranged by size: 4320 1030 667 432 181 SphI G_CATG'C Cuts at: 0 3951 6630 Size: 3951 2679 SspI AAT'ATT Cuts at: 0 5571 6630 Size: 5571 1059 StuI AGG'CCT Cuts at: 0 1941 4285 6630 Size: 1941 2344 2345 Fragments arranged by size: 2345 2344 1941 StyI C'CwwG_G Cuts at: 0 1005 2258 4459 5246 5538 5715 5745 5855 Size: 1005 1253 2201 787 292 177 30 110 Cuts at: 5855 6288 6630 Size: 433 342 Fragments arranged by size: 2201 1253 1005 787 433 342 292 177 110 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 9 274 1713 1932 2537 3678 6568 6630 Size: 9 265 1439 219 605 1141 2890 62 Fragments arranged by size: 2890 1439 1141 605 265 219 62 9 TatI w'GTAC_w Cuts at: 0 624 5189 5261 5977 6515 6605 6630 Size: 624 4565 72 716 538 90 25 Fragments arranged by size: 4565 716 624 538 90 72 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1033 1065 1709 1741 2066 2098 3609 3641 Size: 1033 32 644 32 325 32 1511 32 Cuts at: 3641 3707 3739 4394 4426 5290 5322 6182 6214 Size: 66 32 655 32 864 32 860 32 Cuts at: 6214 6216 6248 6528 6560 6630 Size: 2 32 280 32 70 Fragments arranged by size: 1511 1033 864 860 655 644 325 280 70 66 32 32 32 32 32 32 32 32 32 32 2 XbaI T'CTAG_A Cuts at: 0 6247 6630 Size: 6247 383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 916 6630 Size: 916 5714 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI SgrAI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AatII AflII AflIII AhdI AscI AsiSI BbeI BglI BglII BlpI BsiEI BsiWI BspEI BssHII BstAPI BstBI BstEII BtgZI ClaI EagI EcoRV FseI FspAI HpaI KasI MfeI MluI NaeI NarI NgoMIV NotI NruI PacI PciI PmeI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI XmnI ZraI