Restriction map for
pFN21AE2302
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccII AccIII AgeI
Aor51HI ApaI AscI Asp718I AspI
AvaI AviII BalI BbeI BbvCI
BcgI BcnI BglI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspEI BsrBI BsrFI
BssHII BstEII BstPI BstUI CelII
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
HaeII HapII HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 3482 AACGTT AA^CGTT
AcyI 3716 GGCGTC G[GA]^CG[CT]C
AhdI 1738 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1738 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4377 CCTAGG C^CTAGG
BanII 5480 GGGCTC G[GA]GC[CT]^C
BbrPI 6307 CACGTG CAC^GTG
BclI 5394 TGATCA T^GATCA
BlnI 4377 CCTAGG C^CTAGG
BsaHI 3716 GGCGTC G[GA]^CG[CT]C
BsgI 2014 GTGCAG GTGCAG(16/14)
BsmBI 986 CGTCTC CGTCTC(1/5)
BspDI 5464 ATCGAT AT^CGAT
BssSI 3062 CTCGTG CACGAG(-5/-1)
Bst1107I 556 GTATAC GTA^TAC
BstBI 3836 TTCGAA TT^CGAA
BstXI 1083 CCACCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 556 GTATAC GTA^TAC
CfoI 2632 GCGC GCG^C
ClaI 5464 ATCGAT AT^CGAT
DraIII 925 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3951 TGGCCG [CT]^GGCC[GA]
Eam1105I 1738 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 6109 GCGGG CCCGC(4/6)
FbaI 5394 TGATCA T^GATCA
HhaI 2632 GCGC GCG^C
Hin1I 3716 GGCGTC G[GA]^CG[CT]C
HinP1I 2632 GCGC G^CGC
HpaI 4230 GTTAAC GTT^AAC
NdeI 2090 CATATG CA^TATG
NspV 3836 TTCGAA TT^CGAA
PflMI 4675 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 6307 CACGTG CAC^GTG
PmlI 6307 CACGTG CAC^GTG
PshAI 4375 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3482 AACGTT AA^CGTT
SfuI 3836 TTCGAA TT^CGAA
SnaBI 410 TACGTA TAC^GTA
SpeI 2278 ACTAGT A^CTAGT
SphI 838 GCATGC GCATG^C
SspI 6169 AATATT AAT^ATT
StuI 6290 AGGCCT AGG^CCT
Van91I 4675 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflII 181 CTTAAG C^TTAAG
6286 CTTAAG
Alw44I 2969 GTGCAC G^TGCAC
3585 GTGCAC
AocI 991 CCTGAGG CC^T[ACGT]AGG
6387 CCTCAGG
ApaLI 2969 GTGCAC G^TGCAC
3585 GTGCAC
AseI 693 ATTAAT AT^TAAT
6034 ATTAAT
AsnI 693 ATTAAT AT^TAAT
6034 ATTAAT
BamHI 889 GGATCC G^GATCC
4708 GGATCC
BfrI 181 CTTAAG C^TTAAG
6286 CTTAAG
BstAPI 2056 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3686 GCAATGACTGC
Bsu36I 991 CCTGAGG CC^T[ACGT]AGG
6387 CCTCAGG
DrdI 2323 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6455 GACGCTCATGTC
Eco81I 991 CCTGAGG CC^T[ACGT]AGG
6387 CCTCAGG
EcoRI 2116 GAATTC G^AATTC
2996 GAATTC
EcoRV 3439 GATATC GAT^ATC
6028 GATATC
EcoT22I 1564 ATGCAT ATGCA^T
2093 ATGCAT
HindIII 4158 AAGCTT A^AGCTT
4348 AAGCTT
MfeI 3654 CAATTG C^AATTG
4913 CAATTG
MunI 3654 CAATTG C^AATTG
4913 CAATTG
NsiI 1564 ATGCAT ATGCA^T
2093 ATGCAT
PpuMI 3915 GGGTCCT [GA]G^G[AT]CC[CT]
4380 AGGTCCT
PshBI 693 ATTAAT AT^TAAT
6034 ATTAAT
XbaI 2604 TCTAGA T^CTAGA
5010 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BbsI BbvI
BciVI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI