(Circular) (Six-base) MAPSORT of: pFN21AE2302.seq Check: 8520 from: 1 to: 11227 pFN21AE2302 11227 bp With 182 enzymes: SgfI * November 26, 2010 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6922 6922 Size: 11227 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9037 278 Size: 53 83 186 936 7501 2468 Fragments arranged by size: 7501 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2562 6722 7522 8570 1937 Size: 625 4160 800 1048 4594 Fragments arranged by size: 4594 4160 1048 800 625 Acc65I G'GTAC_C Cuts at: 8548 8548 Size: 11227 AclI AA'CG_TT Cuts at: 1563 5488 9354 9727 1563 Size: 3925 3866 373 3063 Fragments arranged by size: 3925 3866 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 3036 4845 5223 5365 5843 5855 6540 Size: 1143 261 1809 378 142 478 12 685 Cuts at: 6540 6561 7905 8165 8304 8367 9290 10674 10942 Size: 21 1344 260 139 63 923 1384 268 Cuts at: 10942 1632 Size: 1917 Fragments arranged by size: 1917 1809 1384 1344 1143 923 685 478 378 268 261 260 142 139 63 21 12 AflII C'TTAA_G Cuts at: 820 1017 2186 8291 820 Size: 197 1169 6105 3756 Fragments arranged by size: 6105 3756 1169 197 AflIII A'CryG_T Cuts at: 1203 5521 5668 5739 7686 10126 11103 1203 Size: 4318 147 71 1947 2440 977 1327 Fragments arranged by size: 4318 2440 1947 1327 977 147 71 AgeI A'CCGG_T Cuts at: 10058 10058 Size: 11227 AhdI GACnn_n'nnGTC Cuts at: 3748 9956 3748 Size: 6208 5019 AleI CACnn'nnGTG Cuts at: 1166 3093 3438 3624 1166 Size: 1927 345 186 8769 Fragments arranged by size: 8769 1927 345 186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6385 1184 Size: 5201 6026 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4590 4622 7486 7518 4590 Size: 32 2864 32 8299 Fragments arranged by size: 8299 2864 32 32 AlwNI CAG_nnn'CTG Cuts at: 3059 7087 7790 8970 10542 3059 Size: 4028 703 1180 1572 3744 Fragments arranged by size: 4028 3744 1572 1180 703 ApaLI G'TGCA_C Cuts at: 4974 5590 9284 10440 4974 Size: 616 3694 1156 5761 Fragments arranged by size: 5761 3694 1156 616 ApoI r'AATT_y Cuts at: 1493 2407 3044 3589 3914 4121 4731 5001 5116 Size: 914 637 545 325 207 610 270 115 Cuts at: 5116 5251 5402 6436 7514 7942 8536 8762 8914 Size: 135 151 1034 1078 428 594 226 152 Cuts at: 8914 11023 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1078 1034 914 637 610 594 545 428 325 270 226 207 152 151 135 115 AseI AT'TA_AT Cuts at: 160 1032 2699 8040 9781 160 Size: 872 1667 5341 1741 1606 Fragments arranged by size: 5341 1741 1667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11227 AvaI C'yCGr_G Cuts at: 1870 1943 8552 1870 Size: 73 6609 4545 Fragments arranged by size: 6609 4545 73 AvrII C'CTAG_G Cuts at: 6382 6382 Size: 11227 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3798 3831 3798 Size: 33 11194 BamHI G'GATC_C Cuts at: 2894 6713 8557 8924 2894 Size: 3819 1844 367 5197 Fragments arranged by size: 5197 3819 1844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3331 3575 5315 8548 10003 Size: 325 217 627 1544 244 1740 3233 1455 Cuts at: 10003 618 Size: 1842 Fragments arranged by size: 3233 1842 1740 1544 1455 627 325 244 217 BanII G_rGCy'C Cuts at: 1391 7489 8546 1391 Size: 6098 1057 4072 Fragments arranged by size: 6098 4072 1057 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 2271 2958 928 Size: 971 261 111 687 9197 Fragments arranged by size: 9197 971 687 261 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 9418 9452 1903 Size: 34 7481 34 3678 Fragments arranged by size: 7481 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4515 4579 6918 7376 7858 9119 10329 4515 Size: 64 2339 458 482 1261 1210 5413 Fragments arranged by size: 5413 2339 1261 1210 482 458 64 BclI T'GATC_A Cuts at: 1548 7399 1548 Size: 5851 5376 BfrBI ATG'CAT Cuts at: 3571 4100 3571 Size: 529 10698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9838 136 Size: 107 122 71 631 605 8166 1525 Fragments arranged by size: 8166 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4489 6175 7429 11222 4489 Size: 1686 1254 3793 4494 Fragments arranged by size: 4494 3793 1686 1254 BlpI GC'TnA_GC Cuts at: 8644 8644 Size: 11227 Bme1580I G_kGCm'C Cuts at: 1790 4978 5594 9288 10444 1790 Size: 3188 616 3694 1156 2573 Fragments arranged by size: 3694 3188 2573 1156 616 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2265 3748 7159 Size: 416 53 83 399 24 848 1483 3411 Cuts at: 7159 8958 9916 442 Size: 1799 958 1753 Fragments arranged by size: 3411 1799 1753 1483 958 848 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11227 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3492 4617 6819 7290 8067 9887 1239 Size: 2253 1125 2202 471 777 1820 2579 Fragments arranged by size: 2579 2253 2202 1820 1125 777 471 Bpu10I CC'TnA_GC Cuts at: 3366 6520 7546 3366 Size: 3154 1026 7047 Fragments arranged by size: 7047 3154 1026 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3284 5079 6335 8247 9352 10217 10515 3284 Size: 1795 1256 1912 1105 865 298 3996 Fragments arranged by size: 3996 1912 1795 1256 1105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9890 882 Size: 617 8391 2219 Fragments arranged by size: 8391 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2417 5669 6906 8314 9018 493 Size: 711 1213 3252 1237 1408 704 2702 Fragments arranged by size: 3252 2702 1408 1237 1213 711 704 BsaBI GATnn'nnATC Cuts at: 11221 11221 Size: 11227 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5722 9034 9416 10051 Size: 53 83 186 936 4189 3312 382 635 Cuts at: 10051 275 Size: 1451 Fragments arranged by size: 4189 3312 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9660 10058 10332 10479 10890 9660 Size: 398 274 147 411 9997 Fragments arranged by size: 9997 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4590 4620 5739 5769 7021 Size: 30 165 30 3187 30 1119 30 1252 Cuts at: 7021 7051 8053 8083 1178 Size: 30 1002 30 4322 Fragments arranged by size: 4322 3187 1252 1119 1002 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3552 3657 5046 5055 5840 7029 1186 Size: 2366 105 1389 9 785 1189 5384 Fragments arranged by size: 5384 2366 1389 1189 785 105 9 BseYI C'CCAG_C Cuts at: 2839 6429 6788 7128 10430 10926 2839 Size: 3590 359 340 3302 496 3140 Fragments arranged by size: 3590 3302 3140 496 359 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4040 1163 Size: 573 2304 8350 Fragments arranged by size: 8350 2304 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8677 9438 10466 1540 Size: 275 186 6676 761 1028 2301 Fragments arranged by size: 6676 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3169 4978 5095 5594 5828 6971 9288 9373 10444 Size: 1809 117 499 234 1143 2317 85 1071 Cuts at: 10444 3169 Size: 3952 Fragments arranged by size: 3952 2317 1809 1143 1071 499 234 117 85 BsmI GAATG_Cn' Cuts at: 2351 2666 3155 5850 7728 8738 8831 2351 Size: 315 489 2695 1878 1010 93 4747 Fragments arranged by size: 4747 2695 1878 1010 489 315 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2997 2997 Size: 11227 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3169 4978 5095 5594 5828 6971 7489 Size: 399 1379 1809 117 499 234 1143 518 Cuts at: 7489 8546 9288 9373 10444 1391 Size: 1057 742 85 1071 2174 Fragments arranged by size: 2174 1809 1379 1143 1071 1057 742 518 499 399 234 117 85 BspEI T'CCGG_A Cuts at: 10890 10890 Size: 11227 BspHI T'CATG_A Cuts at: 5391 6249 8158 9116 10846 5391 Size: 858 1909 958 1730 5772 Fragments arranged by size: 5772 1909 1730 958 858 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5092 5188 6922 8216 8582 844 Size: 4248 96 1734 1294 366 3489 Fragments arranged by size: 4248 3489 1734 1294 366 96 BsrBI CCG'CTC Cuts at: 1394 1823 9114 1394 Size: 429 7291 3507 Fragments arranged by size: 7291 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2527 3989 5698 7568 7758 9722 9896 Size: 1183 1279 1462 1709 1870 190 1964 174 Cuts at: 9896 65 Size: 1396 Fragments arranged by size: 1964 1870 1709 1462 1396 1279 1183 190 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9871 10058 1700 Size: 110 8061 187 2869 Fragments arranged by size: 8061 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 7063 7555 8074 96 Size: 6967 492 519 3249 Fragments arranged by size: 6967 3249 519 492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11227 BssSI C'ACGA_G Cuts at: 5067 9287 10299 5067 Size: 4220 1012 5995 Fragments arranged by size: 5995 4220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4067 5697 794 Size: 3273 1630 6324 Fragments arranged by size: 6324 3273 1630 BstBI TT'CG_AA Cuts at: 5842 10031 5842 Size: 4189 7038 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11227 BstXI CCAn_nnnn'nTGG Cuts at: 3095 3095 Size: 11227 BstYI r'GATC_y Cuts at: 1972 2894 2953 3703 4394 4489 5341 5629 6095 Size: 922 59 750 691 95 852 288 466 Cuts at: 6095 6175 6260 6713 7429 8087 8557 8924 9309 Size: 80 85 453 716 658 470 367 385 Cuts at: 9309 9326 10778 10864 10876 11222 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 922 852 750 716 691 658 470 466 453 385 367 346 288 95 86 85 80 59 17 12 BstZ17I GTA'TAC Cuts at: 2563 2563 Size: 11227 Bsu36I CC'TnA_GG Cuts at: 2997 8393 2997 Size: 5396 5831 BtgI C'CryG_G Cuts at: 513 1065 3091 7233 7574 8101 513 Size: 552 2026 4142 341 527 3639 Fragments arranged by size: 4142 3639 2026 552 527 341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10488 Fragments arranged by size: 10488 683 56 BtsI GCAGTG_nn' Cuts at: 4482 4923 4978 5828 5993 8743 9537 9557 4482 Size: 441 55 850 165 2750 794 20 6152 Fragments arranged by size: 6152 2750 850 794 441 165 55 20 ClaI AT'CG_AT Cuts at: 1554 7470 1554 Size: 5916 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3108 3143 4503 4538 529 Size: 35 2544 35 1360 35 7218 Fragments arranged by size: 7218 2544 1360 35 35 35 DraI TTT'AAA Cuts at: 3999 5260 7137 8531 3999 Size: 1261 1877 1394 6695 Fragments arranged by size: 6695 1877 1394 1261 DraIII CAC_nnn'GTG Cuts at: 2935 2935 Size: 11227 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4334 8466 10234 809 Size: 507 3018 4132 1768 1802 Fragments arranged by size: 4132 3018 1802 1768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5956 8674 9563 Size: 54 1425 50 275 18 4126 2718 889 Cuts at: 9563 11192 8 Size: 1629 43 Fragments arranged by size: 4126 2718 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8674 1537 Size: 275 6862 4090 Fragments arranged by size: 6862 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2230 2571 4367 4803 5670 5862 6063 Size: 713 164 341 1796 436 867 192 201 Cuts at: 6063 6982 7038 9157 1353 Size: 919 56 2119 3423 Fragments arranged by size: 3423 2119 1796 919 867 713 436 341 201 192 164 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9814 10188 10334 387 Size: 1064 138 8225 374 146 1280 Fragments arranged by size: 8225 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2775 3036 3492 4617 4845 5223 5365 Size: 393 1143 261 456 1125 228 378 142 Cuts at: 5365 5843 5855 6540 6561 6819 7290 7905 8067 Size: 478 12 685 21 258 471 615 162 Cuts at: 8067 8165 8304 8367 9290 9887 10674 10942 1239 Size: 98 139 63 923 597 787 268 1524 Fragments arranged by size: 1524 1143 1125 923 787 685 615 597 478 471 456 393 378 268 261 258 228 162 142 139 98 63 21 12 EcoO109I rG'GnC_Cy Cuts at: 4865 5921 6386 4865 Size: 1056 465 9706 Fragments arranged by size: 9706 1056 465 EcoRI G'AATT_C Cuts at: 4121 5001 8536 8914 4121 Size: 880 3535 378 6434 Fragments arranged by size: 6434 3535 880 378 EcoRV GAT'ATC Cuts at: 5446 8035 5446 Size: 2589 8638 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6477 6477 Size: 11227 FspI TGC'GCA Cuts at: 9733 9733 Size: 11227 HaeII r_GCGC'y Cuts at: 1678 10374 1678 Size: 8696 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3008 3040 3483 3490 3515 Size: 32 135 32 1436 32 443 7 25 Cuts at: 3515 3522 3734 3766 4184 4216 4223 4255 4441 Size: 7 212 32 418 32 7 32 186 Cuts at: 4441 4454 4473 4486 5726 5758 6760 6792 7021 Size: 13 19 13 1240 32 1002 32 229 Cuts at: 7021 7053 7778 7810 8119 8151 8447 8479 9868 Size: 32 725 32 309 32 296 32 1389 Cuts at: 9868 9900 9942 9974 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1436 1389 1240 1002 725 443 418 309 296 229 212 186 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 19 13 13 7 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3677 6212 6237 7797 8571 8817 Size: 988 281 1739 2535 25 1560 774 246 Cuts at: 8817 669 Size: 3079 Fragments arranged by size: 3079 2535 1739 1560 988 774 281 246 25 HindIII A'AGCT_T Cuts at: 748 6163 6353 748 Size: 5415 190 5622 Fragments arranged by size: 5622 5415 190 HpaI GTT'AAC Cuts at: 6237 8817 6237 Size: 2580 8647 KpnI G_GTAC'C Cuts at: 8552 8552 Size: 11227 MfeI C'AATT_G Cuts at: 5659 6918 8826 5659 Size: 1259 1908 8060 Fragments arranged by size: 8060 1908 1259 MluI A'CGCG_T Cuts at: 11103 11103 Size: 11227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3913 4811 4819 5057 5305 5369 6238 6660 Size: 2608 898 8 238 248 64 869 422 Cuts at: 6660 7151 7208 7472 8084 10341 10525 1305 Size: 491 57 264 612 2257 184 2007 Fragments arranged by size: 2608 2257 2007 898 869 612 491 422 264 248 238 184 64 57 8 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 9405 Fragments arranged by size: 9405 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3093 3438 3581 3624 5152 5526 6834 Size: 648 1927 345 143 43 1528 374 1308 Cuts at: 6834 7573 8106 9185 9544 9703 518 Size: 739 533 1079 359 159 2042 Fragments arranged by size: 2042 1927 1528 1308 1079 739 648 533 374 359 345 159 143 43 MspA1I CmG'CkG Cuts at: 1606 3224 4434 4992 6320 6788 8643 9320 10468 Size: 1618 1210 558 1328 468 1855 677 1148 Cuts at: 10468 10713 1606 Size: 245 2120 Fragments arranged by size: 2120 1855 1618 1328 1210 1148 677 558 468 245 NcoI C'CATG_G Cuts at: 513 1065 3091 7233 7574 8101 513 Size: 552 2026 4142 341 527 3639 Fragments arranged by size: 4142 3639 2026 552 527 341 NdeI CA'TA_TG Cuts at: 387 4096 387 Size: 3709 7518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11227 NotI GC'GGCC_GC Cuts at: 8674 8674 Size: 11227 NsiI A_TGCA'T Cuts at: 3573 4102 3573 Size: 529 10698 NspI r_CATG'y Cuts at: 1132 1229 2847 5157 5525 5743 6899 7690 8259 Size: 97 1618 2310 368 218 1156 791 569 Cuts at: 8259 8585 10130 1132 Size: 326 1545 2229 Fragments arranged by size: 2310 2229 1618 1545 1156 791 569 368 326 218 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 11227 PciI A'CATG_T Cuts at: 5521 5739 7686 10126 5521 Size: 218 1947 2440 6622 Fragments arranged by size: 6622 2440 1947 218 PflMI CCAn_nnn'nTGG Cuts at: 1108 6686 1108 Size: 5578 5649 PfoI T'CCnGG_A Cuts at: 8083 8083 Size: 11227 PmeI GTTT'AAAC Cuts at: 8531 8531 Size: 11227 PmlI CAC'GTG Cuts at: 8314 8314 Size: 11227 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4590 4622 9296 9328 10809 10841 4590 Size: 32 4674 32 1481 32 4976 Fragments arranged by size: 4976 4674 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5921 6386 5921 Size: 465 10762 PshAI GACnn'nnGTC Cuts at: 1613 6384 1613 Size: 4771 6456 PsiI TTA'TAA Cuts at: 8797 8797 Size: 11227 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3845 3877 3845 Size: 32 11195 PstI C_TGCA'G Cuts at: 830 2803 4489 6374 6532 6712 7717 8579 830 Size: 1973 1686 1885 158 180 1005 862 3478 Fragments arranged by size: 3478 1973 1885 1686 1005 862 180 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11227 PvuII CAG'CTG Cuts at: 3224 4434 6320 6788 3224 Size: 1210 1886 468 7663 Fragments arranged by size: 7663 1886 1210 468 SalI G'TCGA_C Cuts at: 1936 8569 1936 Size: 6633 4594 SapI GCTCTTCn'nnn_ Cuts at: 2571 5670 6063 2571 Size: 3099 393 7735 Fragments arranged by size: 7735 3099 393 SbfI CC_TGCA'GG Cuts at: 8579 8579 Size: 11227 ScaI AGT'ACT Cuts at: 3082 7054 8131 9475 11187 3082 Size: 3972 1077 1344 1712 3122 Fragments arranged by size: 3972 3122 1712 1344 1077 SfcI C'TryA_G Cuts at: 826 1045 2799 3783 3792 4485 4669 6370 6528 Size: 219 1754 984 9 693 184 1701 158 Cuts at: 6528 6708 6779 7523 7713 8575 9710 10391 10582 Size: 180 71 744 190 862 1135 681 191 Cuts at: 10582 826 Size: 1471 Fragments arranged by size: 1754 1701 1471 1135 984 862 744 693 681 219 191 190 184 180 158 71 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11227 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11227 SmaI CCC'GGG Cuts at: 1872 8554 1872 Size: 6682 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 3299 5094 6350 8262 8291 Size: 197 926 243 1113 1795 1256 1912 29 Cuts at: 8291 9331 10232 10494 820 Size: 1040 901 262 1553 Fragments arranged by size: 1912 1795 1553 1256 1113 1040 926 901 262 243 197 29 SnaBI TAC'GTA Cuts at: 493 2417 9018 493 Size: 1924 6601 2702 Fragments arranged by size: 6601 2702 1924 SpeI A'CTAG_T Cuts at: 152 4283 152 Size: 4131 7096 SphI G_CATG'C Cuts at: 1132 2847 8585 1132 Size: 1715 5738 3774 Fragments arranged by size: 5738 3774 1715 SspI AAT'ATT Cuts at: 5 52 8176 9151 5 Size: 47 8124 975 2081 Fragments arranged by size: 8124 2081 975 47 StuI AGG'CCT Cuts at: 1517 8297 1517 Size: 6780 4447 StyI C'CwwG_G Cuts at: 513 1065 3091 3708 5039 5048 5906 6382 7233 Size: 552 2026 617 1331 9 858 476 851 Cuts at: 7233 7574 8101 8197 8666 513 Size: 341 527 96 469 3074 Fragments arranged by size: 3074 2026 1331 858 851 617 552 527 476 469 341 96 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 8376 9254 9271 9424 9609 10054 Size: 1255 1332 5464 878 17 153 185 445 Cuts at: 10054 325 Size: 1498 Fragments arranged by size: 5464 1498 1332 1255 878 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2197 3080 6121 Size: 274 80 33 51 1443 220 883 3041 Cuts at: 6121 7052 7063 7555 7874 8074 8129 8412 9473 Size: 931 11 492 319 200 55 283 1061 Cuts at: 9473 11185 96 Size: 1712 138 Fragments arranged by size: 3041 1712 1443 1061 931 883 492 319 283 274 220 200 138 80 55 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2938 2970 4189 4221 5053 5085 5780 5812 5985 Size: 32 1219 32 832 32 695 32 173 Cuts at: 5985 6017 11187 11219 2938 Size: 32 5170 32 2946 Fragments arranged by size: 5170 2946 1219 832 695 173 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11227 XbaI T'CTAG_A Cuts at: 4609 7015 8563 4609 Size: 2406 1548 7273 Fragments arranged by size: 7273 2406 1548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6013 7942 8238 6013 Size: 1929 296 9002 Fragments arranged by size: 9002 1929 296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11227 XmaI C'CCGG_G Cuts at: 1870 8552 1870 Size: 6682 4545 XmnI GAAnn'nnTTC Cuts at: 2717 4621 5413 9356 2717 Size: 1904 792 3943 4588 Fragments arranged by size: 4588 3943 1904 792 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9035 276 Size: 53 83 186 936 7501 2468 Fragments arranged by size: 7501 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BplI BsiWI EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI