(Linear) (Six-base) MAPSORT of: KIEE2302.seq Check: 2032 from: 1 to: 6522 KIEE2302.orf With 182 enzymes: SgfI * November 26, 2010 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4917 6522 Size: 4917 1605 AccI GT'mk_AC Cuts at: 0 557 4717 5517 6522 Size: 557 4160 800 1005 Fragments arranged by size: 4160 1005 800 557 AclI AA'CG_TT Cuts at: 0 3483 6522 Size: 3483 3039 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 770 1031 2840 3218 3360 3838 3850 4535 Size: 770 261 1809 378 142 478 12 685 Cuts at: 4535 4556 5900 6160 6299 6362 6522 Size: 21 1344 260 139 63 160 Fragments arranged by size: 1809 1344 770 685 478 378 261 260 160 142 139 63 21 12 AflII C'TTAA_G Cuts at: 0 181 6286 6522 Size: 181 6105 236 Fragments arranged by size: 6105 236 181 AflIII A'CryG_T Cuts at: 0 3516 3663 3734 5681 6522 Size: 3516 147 71 1947 841 Fragments arranged by size: 3516 1947 841 147 71 AhdI GACnn_n'nnGTC Cuts at: 0 1743 6522 Size: 1743 4779 AleI CACnn'nnGTG Cuts at: 0 1088 1433 1619 6522 Size: 1088 345 186 4903 Fragments arranged by size: 4903 1088 345 186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4380 6522 Size: 4380 2142 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2585 2617 5481 5513 6522 Size: 2585 32 2864 32 1009 Fragments arranged by size: 2864 2585 1009 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1054 5082 5785 6522 Size: 1054 4028 703 737 Fragments arranged by size: 4028 1054 737 703 ApaLI G'TGCA_C Cuts at: 0 2969 3585 6522 Size: 2969 616 2937 Fragments arranged by size: 2969 2937 616 ApoI r'AATT_y Cuts at: 0 402 1039 1584 1909 2116 2726 2996 3111 Size: 402 637 545 325 207 610 270 115 Cuts at: 3111 3246 3397 4431 5509 5937 6522 Size: 135 151 1034 1078 428 585 Fragments arranged by size: 1078 1034 637 610 585 545 428 402 325 270 207 151 135 115 AseI AT'TA_AT Cuts at: 0 694 6035 6522 Size: 694 5341 487 Fragments arranged by size: 5341 694 487 AvrII C'CTAG_G Cuts at: 0 4377 6522 Size: 4377 2145 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1793 1826 6522 Size: 1793 33 4696 Fragments arranged by size: 4696 1793 33 BamHI G'GATC_C Cuts at: 0 889 4708 6522 Size: 889 3819 1814 Fragments arranged by size: 3819 1814 889 BanI G'GyrC_C Cuts at: 0 1326 1570 3310 6522 Size: 1326 244 1740 3212 Fragments arranged by size: 3212 1740 1326 244 BanII G_rGCy'C Cuts at: 0 5484 6522 Size: 5484 1038 BbsI GAAGACnn'nnnn_ Cuts at: 0 155 266 953 6522 Size: 155 111 687 5569 Fragments arranged by size: 5569 687 155 111 BciVI GTATCCnnnnn_n' Cuts at: 0 2510 2574 4913 5371 5853 6522 Size: 2510 64 2339 458 482 669 Fragments arranged by size: 2510 2339 669 482 458 64 BclI T'GATC_A Cuts at: 0 5394 6522 Size: 5394 1128 BfrBI ATG'CAT Cuts at: 0 1566 2095 6522 Size: 1566 529 4427 Fragments arranged by size: 4427 1566 529 BglII A'GATC_T Cuts at: 0 2484 4170 5424 6522 Size: 2484 1686 1254 1098 Fragments arranged by size: 2484 1686 1254 1098 Bme1580I G_kGCm'C Cuts at: 0 2973 3589 6522 Size: 2973 616 2933 Fragments arranged by size: 2973 2933 616 BmrI ACTGGGnnnn_n' Cuts at: 0 260 1743 5154 6522 Size: 260 1483 3411 1368 Fragments arranged by size: 3411 1483 1368 260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1487 2612 4814 5285 6062 6522 Size: 1487 1125 2202 471 777 460 Fragments arranged by size: 2202 1487 1125 777 471 460 Bpu10I CC'TnA_GC Cuts at: 0 1361 4515 5541 6522 Size: 1361 3154 1026 981 Fragments arranged by size: 3154 1361 1026 981 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1279 3074 4330 6242 6522 Size: 1279 1795 1256 1912 280 Fragments arranged by size: 1912 1795 1279 1256 280 BsaAI yAC'GTr Cuts at: 0 412 3664 4901 6309 6522 Size: 412 3252 1237 1408 213 Fragments arranged by size: 3252 1408 1237 412 213 BsaHI Gr'CG_yC Cuts at: 0 3717 6522 Size: 3717 2805 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2585 2615 3734 3764 5016 5046 6048 6078 Size: 2585 30 1119 30 1252 30 1002 30 Cuts at: 6078 6522 Size: 444 Fragments arranged by size: 2585 1252 1119 1002 444 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1547 1652 3041 3050 3835 5024 6522 Size: 1547 105 1389 9 785 1189 1498 Fragments arranged by size: 1547 1498 1389 1189 785 105 9 BseYI C'CCAG_C Cuts at: 0 834 4424 4783 5123 6522 Size: 834 3590 359 340 1399 Fragments arranged by size: 3590 1399 834 359 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2035 6522 Size: 2035 4487 BsiHKAI G_wGCw'C Cuts at: 0 1164 2973 3090 3589 3823 4966 6522 Size: 1164 1809 117 499 234 1143 1556 Fragments arranged by size: 1809 1556 1164 1143 499 234 117 BsmI GAATG_Cn' Cuts at: 0 346 661 1150 3845 5723 6522 Size: 346 315 489 2695 1878 799 Fragments arranged by size: 2695 1878 799 489 346 315 BsmBI CGTCTCn'nnnn_ Cuts at: 0 992 6522 Size: 992 5530 Bsp1286I G_dGCh'C Cuts at: 0 1164 2973 3090 3589 3823 4966 5484 6522 Size: 1164 1809 117 499 234 1143 518 1038 Fragments arranged by size: 1809 1164 1143 1038 518 499 234 117 BspHI T'CATG_A Cuts at: 0 3386 4244 6153 6522 Size: 3386 858 1909 369 Fragments arranged by size: 3386 1909 858 369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3087 3183 4917 6211 6522 Size: 3087 96 1734 1294 311 Fragments arranged by size: 3087 1734 1294 311 96 BsrDI GCAATG_nn' Cuts at: 0 522 1984 3693 5563 5753 6522 Size: 522 1462 1709 1870 190 769 Fragments arranged by size: 1870 1709 1462 769 522 190 BsrGI T'GTAC_A Cuts at: 0 5058 5550 6069 6522 Size: 5058 492 519 453 Fragments arranged by size: 5058 519 492 453 BssSI C'ACGA_G Cuts at: 0 3062 6522 Size: 3062 3460 BstAPI GCAn_nnn'nTGC Cuts at: 0 2062 3692 6522 Size: 2062 1630 2830 Fragments arranged by size: 2830 2062 1630 BstBI TT'CG_AA Cuts at: 0 3837 6522 Size: 3837 2685 BstXI CCAn_nnnn'nTGG Cuts at: 0 1090 6522 Size: 1090 5432 BstYI r'GATC_y Cuts at: 0 889 948 1698 2389 2484 3336 3624 4090 Size: 889 59 750 691 95 852 288 466 Cuts at: 4090 4170 4255 4708 5424 6082 6522 Size: 80 85 453 716 658 440 Fragments arranged by size: 889 852 750 716 691 658 466 453 440 288 95 85 80 59 BstZ17I GTA'TAC Cuts at: 0 558 6522 Size: 558 5964 Bsu36I CC'TnA_GG Cuts at: 0 992 6388 6522 Size: 992 5396 134 Fragments arranged by size: 5396 992 134 BtgI C'CryG_G Cuts at: 0 1086 5228 5569 6096 6522 Size: 1086 4142 341 527 426 Fragments arranged by size: 4142 1086 527 426 341 BtsI GCAGTG_nn' Cuts at: 0 2477 2918 2973 3823 3988 6522 Size: 2477 441 55 850 165 2534 Fragments arranged by size: 2534 2477 850 441 165 55 ClaI AT'CG_AT Cuts at: 0 5465 6522 Size: 5465 1057 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1103 1138 2498 2533 6522 Size: 1103 35 1360 35 3989 Fragments arranged by size: 3989 1360 1103 35 35 DraI TTT'AAA Cuts at: 0 1994 3255 5132 6522 Size: 1994 1261 1877 1390 Fragments arranged by size: 1994 1877 1390 1261 DraIII CAC_nnn'GTG Cuts at: 0 930 6522 Size: 930 5592 DrdI GACnn_nn'nnGTC Cuts at: 0 2329 6461 6522 Size: 2329 4132 61 Fragments arranged by size: 4132 2329 61 EaeI y'GGCC_r Cuts at: 0 3951 6522 Size: 3951 2571 EarI CTCTTCn'nnn_ Cuts at: 0 61 225 566 2362 2798 3665 3857 4058 Size: 61 164 341 1796 436 867 192 201 Cuts at: 4058 4977 5033 6522 Size: 919 56 1489 Fragments arranged by size: 1796 1489 919 867 436 341 201 192 164 61 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 770 1031 1487 2612 2840 3218 3360 3838 Size: 770 261 456 1125 228 378 142 478 Cuts at: 3838 3850 4535 4556 4814 5285 5900 6062 6160 Size: 12 685 21 258 471 615 162 98 Cuts at: 6160 6299 6362 6522 Size: 139 63 160 Fragments arranged by size: 1125 770 685 615 478 471 456 378 261 258 228 162 160 142 139 98 63 21 12 EcoO109I rG'GnC_Cy Cuts at: 0 2860 3916 4381 6522 Size: 2860 1056 465 2141 Fragments arranged by size: 2860 2141 1056 465 EcoRI G'AATT_C Cuts at: 0 2116 2996 6522 Size: 2116 880 3526 Fragments arranged by size: 3526 2116 880 EcoRV GAT'ATC Cuts at: 0 3441 6030 6522 Size: 3441 2589 492 Fragments arranged by size: 3441 2589 492 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4472 6522 Size: 4472 2050 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1003 1035 1478 1485 1510 1517 1729 1761 Size: 1003 32 443 7 25 7 212 32 Cuts at: 1761 2179 2211 2218 2250 2436 2449 2468 2481 Size: 418 32 7 32 186 13 19 13 Cuts at: 2481 3721 3753 4755 4787 5016 5048 5773 5805 Size: 1240 32 1002 32 229 32 725 32 Cuts at: 5805 6114 6146 6442 6474 6522 Size: 309 32 296 32 48 Fragments arranged by size: 1240 1003 1002 725 443 418 309 296 229 212 186 48 32 32 32 32 32 32 32 32 32 32 25 19 13 13 7 7 7 HincII GTy'rAC Cuts at: 0 1672 4207 4232 5792 6522 Size: 1672 2535 25 1560 730 Fragments arranged by size: 2535 1672 1560 730 25 HindIII A'AGCT_T Cuts at: 0 4158 4348 6522 Size: 4158 190 2174 Fragments arranged by size: 4158 2174 190 HpaI GTT'AAC Cuts at: 0 4232 6522 Size: 4232 2290 MfeI C'AATT_G Cuts at: 0 3654 4913 6522 Size: 3654 1259 1609 Fragments arranged by size: 3654 1609 1259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1908 2806 2814 3052 3300 3364 4233 4655 Size: 1908 898 8 238 248 64 869 422 Cuts at: 4655 5146 5203 5467 6079 6522 Size: 491 57 264 612 443 Fragments arranged by size: 1908 898 869 612 491 443 422 264 248 238 64 57 8 MslI CAynn'nnrTG Cuts at: 0 1088 1433 1576 1619 3147 3521 4829 5568 Size: 1088 345 143 43 1528 374 1308 739 Cuts at: 5568 6101 6522 Size: 533 421 Fragments arranged by size: 1528 1308 1088 739 533 421 374 345 143 43 MspA1I CmG'CkG Cuts at: 0 1219 2429 2987 4315 4783 6522 Size: 1219 1210 558 1328 468 1739 Fragments arranged by size: 1739 1328 1219 1210 558 468 NcoI C'CATG_G Cuts at: 0 1086 5228 5569 6096 6522 Size: 1086 4142 341 527 426 Fragments arranged by size: 4142 1086 527 426 341 NdeI CA'TA_TG Cuts at: 0 2091 6522 Size: 2091 4431 NsiI A_TGCA'T Cuts at: 0 1568 2097 6522 Size: 1568 529 4425 Fragments arranged by size: 4425 1568 529 NspI r_CATG'y Cuts at: 0 842 3152 3520 3738 4894 5685 6254 6522 Size: 842 2310 368 218 1156 791 569 268 Fragments arranged by size: 2310 1156 842 791 569 368 268 218 PciI A'CATG_T Cuts at: 0 3516 3734 5681 6522 Size: 3516 218 1947 841 Fragments arranged by size: 3516 1947 841 218 PflMI CCAn_nnn'nTGG Cuts at: 0 4681 6522 Size: 4681 1841 PfoI T'CCnGG_A Cuts at: 0 6078 6522 Size: 6078 444 PmlI CAC'GTG Cuts at: 0 6309 6522 Size: 6309 213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2585 2617 6522 Size: 2585 32 3905 Fragments arranged by size: 3905 2585 32 PpuMI rG'GwC_Cy Cuts at: 0 3916 4381 6522 Size: 3916 465 2141 Fragments arranged by size: 3916 2141 465 PshAI GACnn'nnGTC Cuts at: 0 4379 6522 Size: 4379 2143 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1840 1872 6522 Size: 1840 32 4650 Fragments arranged by size: 4650 1840 32 PstI C_TGCA'G Cuts at: 0 798 2484 4369 4527 4707 5712 6522 Size: 798 1686 1885 158 180 1005 810 Fragments arranged by size: 1885 1686 1005 810 798 180 158 PvuII CAG'CTG Cuts at: 0 1219 2429 4315 4783 6522 Size: 1219 1210 1886 468 1739 Fragments arranged by size: 1886 1739 1219 1210 468 SapI GCTCTTCn'nnn_ Cuts at: 0 566 3665 4058 6522 Size: 566 3099 393 2464 Fragments arranged by size: 3099 2464 566 393 ScaI AGT'ACT Cuts at: 0 1077 5049 6126 6522 Size: 1077 3972 1077 396 Fragments arranged by size: 3972 1077 1077 396 SfcI C'TryA_G Cuts at: 0 794 1778 1787 2480 2664 4365 4523 4703 Size: 794 984 9 693 184 1701 158 180 Cuts at: 4703 4774 5518 5708 6522 Size: 71 744 190 814 Fragments arranged by size: 1701 984 814 794 744 693 190 184 180 158 71 9 SmlI C'TyrA_G Cuts at: 0 181 1294 3089 4345 6257 6286 6522 Size: 181 1113 1795 1256 1912 29 236 Fragments arranged by size: 1912 1795 1256 1113 236 181 29 SnaBI TAC'GTA Cuts at: 0 412 6522 Size: 412 6110 SpeI A'CTAG_T Cuts at: 0 2278 6522 Size: 2278 4244 SphI G_CATG'C Cuts at: 0 842 6522 Size: 842 5680 SspI AAT'ATT Cuts at: 0 6171 6522 Size: 6171 351 StuI AGG'CCT Cuts at: 0 6292 6522 Size: 6292 230 StyI C'CwwG_G Cuts at: 0 1086 1703 3034 3043 3901 4377 5228 5569 Size: 1086 617 1331 9 858 476 851 341 Cuts at: 5569 6096 6192 6522 Size: 527 96 330 Fragments arranged by size: 1331 1086 858 851 617 527 476 341 330 96 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 907 6371 6522 Size: 907 5464 151 Fragments arranged by size: 5464 907 151 TatI w'GTAC_w Cuts at: 0 192 1075 4116 5047 5058 5550 5869 6069 Size: 192 883 3041 931 11 492 319 200 Cuts at: 6069 6124 6407 6522 Size: 55 283 115 Fragments arranged by size: 3041 931 883 492 319 283 200 192 115 55 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 933 965 2184 2216 3048 3080 3775 3807 Size: 933 32 1219 32 832 32 695 32 Cuts at: 3807 3980 4012 6522 Size: 173 32 2510 Fragments arranged by size: 2510 1219 933 832 695 173 32 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 2604 5010 6522 Size: 2604 2406 1512 Fragments arranged by size: 2604 2406 1512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 4008 5937 6233 6522 Size: 4008 1929 296 289 Fragments arranged by size: 4008 1929 296 289 XmnI GAAnn'nnTTC Cuts at: 0 712 2616 3408 6522 Size: 712 1904 792 3114 Fragments arranged by size: 3114 1904 792 712 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AvrII BaeI BamHI BanI BanII BbsI BciVI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsrI PstI PvuII SapI ScaI SfcI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI ApaI AscI AsiSI AvaI BbeI BbvCI BcgI BglI BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstEII BtgZI EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII KasI KpnI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SrfI SwaI Tth111I XhoI XmaI ZraI