################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AcyI AgeI AscI AspI AviII BalI BbeI BsaHI BsiWI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI BstXI ClaI CpoI FseI FspI Hin1I HpaI KasI KspI MluI MluNI MscI NarI NdeI NheI NotI NruI PacI PflFI PinAI PmeI PshAI PvuI RsrII SacII SapI SbfI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 2749 GGTACC G^GTACC AccIII 1181 TCCGGA T^CCGGA AclI 3135 AACGTT AA^CGTT AflII 823 CTTAAG C^TTAAG Aor51HI 1079 AGCGCT AGC^GCT ApaI 3599 GGGCCC GGGCC^C AseI 3331 ATTAAT AT^TAAT AsnI 3331 ATTAAT AT^TAAT Asp718I 2749 GGTACC G^GTACC AvrII 2730 CCTAGG C^CTAGG BamHI 1938 GGATCC G^GATCC BbvCI 2883 GCTGAGG CCTCAGC(-5/-2) BcgI 393 CGAATACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 823 CTTAAG C^TTAAG BlnI 2730 CCTAGG C^CTAGG BsaWI 1181 TCCGGA [AT]^CCGG[AT] BseAI 1181 TCCGGA T^CCGGA BsiEI 118 CGGCCG CG[GA][CT]^CG BsmBI 3610 CGTCTC CGTCTC(1/5) BspEI 1181 TCCGGA T^CCGGA BspHI 1044 TCATGA T^CATGA Bst1107I 4428 GTATAC GTA^TAC BstBI 2996 TTCGAA TT^CGAA BstEII 1200 GGTGACC G^GT[ACGT]ACC BstPI 1200 GGTGACC G^GT[ACGT]ACC BstZ17I 4428 GTATAC GTA^TAC DrdI 4541 GACTTGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 118 CGGCCG C^GGCCG EclXI 118 CGGCCG C^GGCCG Eco47III 1079 AGCGCT AGC^GCT Eco52I 118 CGGCCG C^GGCCG EcoO65I 1200 GGTGACC G^GT[ACGT]ACC EcoT22I 5374 ATGCAT ATGCA^T HaeII 1079 AGCGCT [GA]GCGC^[CT] KpnI 2749 GGTACC GGTAC^C MroI 1181 TCCGGA T^CCGGA NcoI 5648 CCATGG C^CATGG NsiI 5374 ATGCAT ATGCA^T NspV 2996 TTCGAA TT^CGAA PaeR7I 2809 CTCGAG C^TCGAG PshBI 3331 ATTAAT AT^TAAT Psp1406I 3135 AACGTT AA^CGTT PstI 4552 CTGCAG CTGCA^G RcaI 1044 TCATGA T^CATGA SacI 2806 GAGCTC GAGCT^C SalI 4443 GTCGAC G^TCGAC SexAI 4790 ACCAGGT A^CC[AT]GGT SfiI 37 GGCCACGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2996 TTCGAA TT^CGAA SspI 175 AATATT AAT^ATT StuI 4737 AGGCCT AGG^CCT XbaI 3765 TCTAGA T^CTAGA XhoI 2809 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 4428 GTATAC GT^[AC][GT]AC 4443 GTCGAC AccII 56 CGCG CG^CG 1436 CGCG AflIII 2569 ACGTGT A^C[GA][CT]GT 5411 ACACGT AhdI 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3153 GACAATTGGTC Alw44I 2210 GTGCAC G^TGCAC 4951 GTGCAC AlwNI 1777 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 4931 CAGGTGCTG ApaLI 2210 GTGCAC G^TGCAC 4951 GTGCAC AspEI 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3153 GACAATTGGTC BbrPI 2568 CACGTG CAC^GTG 4687 CACGTG BglI 38 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4739 GCCTGGGTGGC BglII 4122 AGATCT A^GATCT 5684 AGATCT BlpI 625 GCTGAGC GC^T[ACGT]AGC 1605 GCTAAGC Bpu1102I 625 GCTGAGC GC^T[ACGT]AGC 1605 GCTAAGC BsaAI 2568 CACGTG [CT]AC^GT[GA] 4687 CACGTG BspMI 2387 ACCTGC ACCTGC(4/8) 81 GCAGGT BstUI 56 CGCG CG^CG 1436 CGCG CelII 625 GCTGAGC GC^T[ACGT]AGC 1605 GCTAAGC DraIII 1549 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4133 CACCCAGTG DsaI 39 CCACGG C^C[GA][CT]GG 5648 CCATGG Eam1105I 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3153 GACAATTGGTC EcoRI 1792 GAATTC G^AATTC 4073 GAATTC FauI 3403 CCCGC CCCGC(4/6) 1561 GCGGG HindIII 1166 AAGCTT A^AGCTT 2860 AAGCTT MvnI 56 CGCG CG^CG 1436 CGCG NaeI 142 GCCGGC GCC^GGC 5198 GCCGGC NgoMIV 142 GCCGGC G^CCGGC 5198 GCCGGC PflMI 1776 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5107 CCACAACTTGG PmaCI 2568 CACGTG CAC^GTG 4687 CACGTG PmlI 2568 CACGTG CAC^GTG 4687 CACGTG ScaI 365 AGTACT AGT^ACT 5468 AGTACT SphI 62 GCATGC GCATG^C 5376 GCATGC Van91I 1776 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5107 CCACAACTTGG XcmI 659 CCAAAAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3095 CCAGTGTGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoRV EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI